Table S2. Genes selected for cloning to investigate their abilities to confer isopentenol tolerance.

Gene / Notes / References / Possible roles1
yhcN / orf, hypothetical protein, cellular response to hydrogen peroxide stress / (1) / O
pphA / protein phosphatase 1 modulates phosphoproteins, signals protein misfolding / (2) / H
ygbA / hypothetical protein, up-regulated by nitrosative stress / (3) / O
yhbW / predicted enzyme, Luciferase family oxidoreductase, suppresses lethality pertinent to σE activity inhibition / (4) / M
nfsA / nitroreductase A, NADPH-dependent, FMN-dependent, upregulated by the redox cycling compound paraquat / (5, 6) / O
ybjC / predicted inner membrane protein, may form an operon with nfsA / (5, 7) / O
metR / DNA-binding transcriptional activator, homocysteine-binding, regulator for metE, which is inactivated by oxidative stress / (8, 9) / O
yjaA / hypothetical protein, deletion strain sensitive to H2O2 and acid / (1) / O
trxC / thioredoxin 2, resistance to nitrosative stress, prevent DNA damage / (10) / O
ibpB / heat shock protein, molecular chaperone / (11, 12) / H
ibpA / heat shock protein, molecular chaperone / (11, 12) / H
bhsA / hypothetical protein, biofilm formation through surface hydrophobicity and stress response / (13) / M
iraD / orf, hypothetical protein, RpoS stabilizer after DNA damage / (14) / D
marA / multiple antibiotic resistance; transcriptional activator of defense systems, marR repressor / (15) / D
nrdI / hypothetical protein, ribonucleotide reductase, has a stimulatory effect on the ribonucleotide reductase activity of NrdH with NrdEF / (16) / D
nrdH / glutaredoxin-like protein, hydrogen donor for NrdEF electron transport system, thioredoxin-like activity / (16) / D
grxA / glutaredoxin 1, redox coenzyme for ribonucleotide reductase (RNR1a) / (17) / D
soxS / DNA-binding transcriptional dual regulator, multiple antibiotic resistance related / (18) / T
dkgA / orf, hypothetical protein,2,5-didehydrogluconate reductase, furfural tolerance / (19) / M
racC / Racprophage; predicted protein, DNA repair / (20) / D
nrdE / ribonucleotide-diphosphate reductase alpha subunit / (21) / D
mdlA / fused predicted multidrug transporter subunits of ABC superfamily: ATP-binding components / (22) / T
sbp / sulfate transporter subunit / (23) / M
mdlB / fused predicted multidrug transporter subunits of ABC superfamily: ATP-binding components / (22) / T
inaA / hypothetical protein, Lipopolysaccharide kinase, pH-inducible protein / (24) / A
ycjF / hypothetical protein, conserved inner membrane protein, heat shock protein / (25) / H
fpr / ferredoxin-NADP reductase, DNA damage repair during soxRS response / (26) / D
aaeX / orf, hypothetical protein, efflux pump / (27) / T
ygbE / conserved inner membrane protein, putative cytochrome oxidase / O
ycjX / conserved protein with nucleoside triphosphate hydrolase domain, heat shock protein / (25) / H
mdtG / predicted drug efflux system, MFS transporter / (28) / T
nrdF / ribonucleotide-diphosphate reductase beta subunit / (21) / D
htpG / heat shock protein 90 / (29) / H
gidB / glucose-inhibited division protein B, Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division / (30-32) / M
rimK / ribosomal protein S6 modification protein, / (33, 34) / M
clpB / protein disaggregation chaperone, heat shock protein / (35) / H
erpA / hypothetical protein,iron-sulfur cluster assembly accessory protein, / (36) / M
poxB / pyruvate dehydrogenase, acetate producing, contributes to aerobic growth efficiency / (37) / M
lon / DNA-binding ATP-dependent protease La, heat shock K-protein / (38, 39) / H
yqhD / alcohol dehydrogenase, NAD(P)-dependent, Uncharacterized oxidoreductases, Fe-dependent ADH family, involved in resistance to reactive oxygen species-generating compounds / (40, 41) / M