BIOLOGY CH13 TEST REVIEW

VOCABULARYCHAPTER 13: DNA, RNA, and Proteins

The Central Dogma of Biology DNA is transcribed into RNA->RNA is translated into Proteins

Griffith - Discovered the transformation of harmless R strain Bacteria by heat killed S bacteria

Avery – Identified DNA as the transformative agent

Hershey & Chase –Studied bacteriophages and proved DNA was the source of hereditary information

James Watson & Francis Crick deduced the structure of DNA

Rosalind Franklin used x ray crystallography to image the structure of DNA

DNA- Deoxyribonucleic Acid

DNA- is a polymer of Nucleotides- remember a polymer is like a chain of beads

DNA- is made of 2 chains, twisted together and coiled into an alpha double helix

DNA- Double Helix is composed of 2 interconnected nucleotide chains

Nucleotide Chains are composed of a Sugar-Phosphate backbone, with bases in the middle

Phosphodiester bonds join nucleotides

Nucleotide contains 1 Nitrogenous base + 1 phosphate group + 1 sugar

Deoxyribosethe sugar in DNA

4 different Nucleotides abbreviated as A, T G, or C

The pyrimidines-Adenine Guanine

The purines -Cytosine Thymine

Chargraff’s Rules - A binds T, G binds C according to

Hydrogen bonds.- join the bases across the helix

MITOSIS-Cell division

MEIOSISgamete formation

DNA Replication- is semi conservative!

Helicase breaks hydrogen bonds in the middle of the strand, creating a replication fork

DNA Polymerase, creates 2 identical DNA molecules

Transcription- DNA copied to RNA, occurs in nucleus

RNA- A nucleic acid that is similar to DNA, Ribose is the sugar, instead of thymine, URACIL, single stranded

Steps of Transcription

RNAPolymerase(RNApol)– Unwinds DNA strand and copies it into RNA

Uracil RNA base replacing Thymine

Messenger RNA (mRNA) DNA transcript or copy moves to the ribosome in the cytoplasm (or on the Rough ER)

Translation (Protein Synthesis)- The Conversion of mRNA to Amino Acids which make up proteins

The Genetic code (ATCG) is translated into a amino acids

CODON-a 3 letter sequence of mRNA that codes for a particular Amino Acid

Amino Acid-the components that make Proteins

tRNA (transferRNA)- the RNA that carries amino acids to the ribosome

Anti-Codon – the 3 letter sequence of tRNA that corresponds to the mRNA CODON

AUG- the start codon

Translation – conversion of mRNA into a Protein

Initiation- Ribosome attaches to mRNA (each 3 bases is called a codon), tRNA brings anticodon and AA to ribosome

Elongation- More tRNA’s bring more AA’s to ribosome, AA’s connected together to make polypeptides (by peptide bonds)

Termination- Ribosome reaches a STOP signal on mRNA

Enzymes- the most common type of protein