California State Polytechnic University, Pomona

Environmental Health & Safety

Select Agents and Toxins

(Revised 10/30/2012)

If you plan on using any of these agents and/or toxins, please contact the Environmental Health & Safety Department at (909) 869-4697 or via eMail at . These agents and/or toxins are heavily regulated by the Center for Disease Control (CDC) and Animal & Plant Health Inspection Service (APHIS). Failure to comply with the laws governing the use of these agents and/or toxins can result in incarceration, fines and even the death penalty.

Viruses

  1. Crimean-Congo haemorrhagic fever virus
  2. Eastern Equine Encephalitis virus (Except South African type) 1
  3. Nipah virus
  4. Hendra virus 1
  5. Ebola viruses5
  6. Lassa fever virus
  7. Lujo Virus
  8. Marburg virus5
  9. Monkeypox virus (Except West African clade)
  10. Rift Valley fever virus 1
  11. Severe Accute Respiratory Syndrome (SARS)-associated coronavirus
  12. South American Haemorrhagic fever viruses (Chapare, Junin, Machupo, Sabia, Guanarito)
  13. Far Eastern Tick-borne encephalitis complex (flavi) viruses [Far Eastern subtype, Siberian subtype, Kyasanur Forest disease virus and Omsk hemorrhagic fever virus)
  14. Variola major virus (Smallpox virus)5
  15. Variola minor virus (Alastrim)5
  16. Venezuelan Equine Encephalitis virus (Excluding any subtypes of Venezuelan equine encephalitis virus except for Subtypes IAB or IC) 1
  17. Reconstructed replication competent forms of the 1918 pandemic influenza virus containing any portion of the coding regions of all eight gene segments

Bacteria

  1. Rickettsia prowazekii
  2. Yersinia pestis5
  3. Bacillus anthracis (Excluding Pasteur strain) 1, 5
  4. Bacillus anthracis1
  5. Brucella abortus 1
  6. Brucella melitensis 1
  7. Brucella suis 1
  8. Burkholderia mallei (formerly Pseudomonas mallei) 1, 5
  9. Burkholderia pseudomallei (formerly Pseudomonas psuedomallei) 1, 5
  10. Botulinum neurotixin producing species of Clostridium 1, 5
  11. Coxiella burnetii 1
  12. Francisella tularensis 1, 5

California State Polytechnic University, Pomona

Environmental Health & Safety

Select Agents and Toxins

Page 2 of 3

Toxins

  1. Abrin (< 100 mg Exemption 4)
  2. Botulinum neurotoxins 1(< 0.5 mg Exemption 4)5
  3. Conotoxins (Designated as short, paralytic alpha conotoxins containing the following amino acid sequence X1CCX2PACGX3X4X5X6CX7()(< 100 mg Exemption 4)
  4. Diacetoxyscirpenol (< 1,000 mg Exemption 4)
  5. Ricin (< 100 mg Exemption 4)
  6. Saxitoxin (< 100 mg Exemption 4)
  7. Staphylococcal enterotoxinsA,B,C,D,E subtypes 1(< 5 mg Exemption 4)
  8. Tetrodotoxin(< 100 mg Exemption 4)
  9. T-2 toxin 1(< 1,000 mg Exemption 4)

Other Agents

  1. Select agent viral nucleic acids (synthetic or naturally derived, contiguous or fragmented, in host chromosomes or in expression vectors) that can encode infectious and/or replication competent forms of any of the select agent viruses.
  2. Nucleic acids (synthetic or naturally derived) that encode for the functional form(s) of any of the toxins if the nucleic acids: (i) Are in a vector or host chromosome; (ii) Can be expressed in vivo or in vitro; or (iii) Are in a vector or host chromosome and can be expressed in vivo or in vitro.
  3. Viruses, bacteria, fungi, and toxins listed above that have been genetically modified. This does not include USDA High Consequence Livestock Pathogens and Toxins.

USDA High Consequence Livestock Pathogens and Toxins

  1. African Horse Sickness Virus 3
  2. African Swine Fever Virus 3
  3. Avain Influenza Virus (Highly Pathogenic) 3
  4. Classical Swine Fever 3
  5. Foot & Mouth Disease Virus 3, 5
  6. Goat Pox Virus 3
  7. Lumpy Skin Disease Virus 3
  8. Mycoplasma Capricolum/m.f 38/m Mycoides Capri (Contagious Caprine Pleuropeumonia Agent) 3
  9. Mycoplasma Mycoides Mycoides (Contagious Bovine Pleuropeumonia Agent)
  10. Newcastle Disease Virus (Velogenic) 3
  11. Peste Des Petits Ruminants 3
  12. Rinderpest Virus 3, 5
  13. Sheep Fox Virus 3
  14. Swine Vesicular Disease Virus 3

California State Polytechnic University, Pomona

Environmental Health & Safety

Select Agents and Toxins

Page 3 of 3

Listed Plant Pathogens

  1. Candidatus Liberobacter africanus 3
  2. Candidatus Liberobacter asiaticus 3
  3. Peronosclerospora philippinensis 3
  4. Phoma glycinicola (formerly Pyrenochaeta glycines)
  5. Ralstonia solanacearum 3
  6. Rathayibacter toxicus
  7. Schlerophthora rayssiae 3
  8. Synchytrium endobioticum 3
  9. Xanthomonas oryzae 3

Footnotes:

  1. Overlap Select Agent
  2. Listed as a Select Agent in 42 CFR 72 Appendix A but not listed as a Select Agent in 42 CFR 73.4
  3. Included on CDC Lists. However, Not listed as a Select Agent in 42 CFR 72 Appendix A or 42 CFR 73.4
  4. Exempt: If under the control of a principal investigator, treating physician or veterinarian, or commercial manufacturer or distributor, if the aggregate amount does not, at any time, exceed the following amount: 100 mg of Abrin; 100 mg of Conotoxins (Short, paralytic alpha conotoxins containing the followingamino acid sequence X1CCX2PACGX3X4X5X6CX7); 1,000 mg of Diacetoxyscirpenol; 100 mg of Ricin; 100 mg of Saxitoxin; or 100 mg of Tetrodotoxin; 0.5 mg of Botulinum neurotoxins or 1,000 mg of T–2 toxin. (Reference: Section 73.3(d)(3) and 73.4(d)(3), 42 CFR)
  5. “Tier 1 “ Select Agent and/or Toxin. Enhanced physical security standards, suitability assessments of lab workers before they can work with Tier 1 agents, and ongoing monitoring of personnel with access to agents or toxins on the Tier 1 list apply. Tier 1 Select Agent and/or Toxin are;
  • Botulinum neurotoxin
  • Toxin-producing strains of Clostridium botulinum
  • Bacillus antracis (excluding Pasteur strain)
  • Burkholderia mallei
  • Burkholderia psudomallei
  • Ebola virus
  • Foot-and-mouth disease virus
  • Francisella tularensis
  • Marburg virus
  • Rinderpest virus
  • Variola major virus
  • Variola minor virus
  • Yersinia pestis