Confidential Do Not Circulate

Supplementary Table S1.Sequencing primers for ADH1B and ADH1C

Name / Region / Primer Sequence (5'-3')
ADH1B-P1 / Promotor1 / Forward / AATGAAATTCAAGAACATCTCTGACCAG
Reverse / TGGAAAGGTATGACGGAAAGAAGA
ADH1B-P2 / Promotor2 / Forward / AGCATGATGAGCAAGAGCTTGAAT
Reverse / GGCTTTCCAAGCATAGGAGTTTGA
ADH1B-P3 / Promotor3 / Forward / TACTGAAATGTTCCCTTGGCAATT
Reverse / GCGTCATTTTAAAGGACTGTTTGTGA
ADH1B-P4 / Promotor4 / Forward / AGGGTGCTCCTATCTGCAAAA
Reverse / CACCCACTGGATTATTCCCAG
ADH1B-P5 / Exon1 / Forward / TGGGAATAATCCAGTGGGTGTGGC
Reverse / AAGAATAGCTTATTTCCACTGCAGT
ADH1B-P6 / Exon2 / Forward / AGACACAACTTATGACTTCAAG
Reverse / CTTTCCTTGACAACAAATGTAA
ADH1B-P7 / Exon3 / Forward / TTTTAAAGCGTGCATTCTTACATAA
Reverse / CGGTACATAATGGTTGAAGGG
ADH1B-P8 / Exon4 / Forward / CTGTGGCAGGCATTGAAAGATGACAT
Reverse / CAAAGTCTGTGCAAAGAGAGCATCAG
ADH1B-P9 / Exon5 / Forward / TAAAGTCATCCCGCTCTTTACTCCTCAGTG
Reverse / TTGCTTCCCTTTTGGTTTCCGACAGT
ADH1B-P10 / Exon4-5 / Forward / TGTGGCAGGCATTGAAAGATGACATA
Reverse / TGAGACAGGAGCATAAGTAATGTGCA
ADH1B-P11 / Exon5 / Forward / TAGGTTTCTGATGCTCTCTTTGCACA
Reverse / CCTTTTGGTTTCCGACAGTCTGCA
ADH1B-P12 / Exon7 / Forward / GAATCCACAGCACTATCCCGT
Reverse / GGTCCTGGGTTATGGTGACAA
ADH1B-P13 / Exon8 / Forward / TATAGCAGTCTGGAATGGAGCACC
Reverse / GGTAATGGAAAACCAAGGGACTCTAT
ADH1B-P14 / Exon9.1 / Forward / CAGATTTTCACTAAGACACCTCAGAT
Reverse / ACTCTTGGACCTTCACTACTTGTAAA
ADH1B-P15 / Exon9.2 / Forward / CTGGGCCATTGTGATTGAAGTCTT
Reverse / CAATGAAGCACTTTCATCAGCAAA
ADH1B-P16 / Exon9.3 / Forward / AGCCTCAATTACTAATTGTAAA
Reverse / GCATTAACAAAGCATTAACACA
ADH1B-P17 / Exon9.4 / Forward / GTAATATGGTGGCTTTAAGTGTAG
Reverse / TGGTTAAGCTGTAATGGATTGG
ADH1C-P1 / Promoter1 / Forward / CGTCCAGAGTTGTTTGTTCCT
Reverse / GCAGTCATCGCCCAATTC
ADH1C-P2 / Promoter2 / Forward / TTCTCCAAGTCCCCACCTG
Reverse / GGCCTAGACCTCATCACTGCT
ADH1C-P3 / Promoter3 / Forward / TTCCTGTAAATGCTGGGAGGCA
Reverse / CGAACGTGGGAGTTGTTTTGCA
ADH1C-P4 / Promoter4 / Forward / TTAACTTAGAGTTGGTATAGATGGCTCCTT
Reverse / GAAGGTCCTCTCTGTAACCCTATAACACT
ADH1C-P5 / Promoter5 / Forward / GATAGGAAGGCTACAGGTTGTCAGTG
Reverse / CCAAGTCCCTACCTGACCCAGA
ADH1C-P6 / Promoter6 / Forward / GTCTCTCCCTCTCTGTCTTTGACTTTG
Reverse / TGGGCAGCAGCAAGATTTATTG
ADH1C-P7 / Promoter7 / Forward / CCACCCAAGACAGCAGCAGCAA
Reverse / GCAGTATAGGGAAGCGGTTATG
ADH1C-P8 / Promoter8 / Forward / TCATTCTCACCTTAAAGACGAGGAAA
Reverse / TTCCCTTTGGGGTCATTCAGAA
ADH1C-P9 / Promoter9 / Forward / TTCCACTTTGTTTATCCAATTTCCC
Reverse / ACTTGTGGATTTCTTCTCTGCTTGA
ADH1C-P10 / Exon1 / Forward / ATAAATACCTGTGCAACATACC
Reverse / GCATATCATATTCCAGTGTATC
ADH1C-P11 / Exon2 / Forward / GGACTCAATGTTGCATGTACAAA
Reverse / CCTTCCTCTTTTTCCCTTGAA
ADH1C-P12 / Exon3 / Forward / TGGGTTTGTTAAGTCCATCTGACAGTC
Reverse / ACCTTGTGCAAGTCCTTTCGTC
ADH1C-P13 / Exon4 / Forward / GACACATAATTCTAATCTATACATGTGAGCG
Reverse / TTCTAGCCTGTGCTCTCAGTTCTTT
ADH1C-P14 / Exon6.1 / Forward / GACTACTGAACAAAAATGAAGAAAGG
Reverse / ACAGCACAGGTAGACCCTGG
ADH1C-P15 / Exon6.2 / Forward / AGGTTTCGATAACAGATTGGACAG
Reverse / CAGGTAACAAAAAGATGACGGG
ADH1C-P16 / Exon71 / Forward / GACATGATCTCTTCCCTCTACA
Reverse / CCACTGCACTCTACCTTGTGTG
ADH1C-P17 / Exon8 / Forward / TAGCAGTCTGGAATGCAGCAC
Reverse / GCAATGGAAAACCAAGGCA
ADH1C-P18 / Exon9 / Forward / TTCACTAAGTCACCTCAAATACTCCTTGG
Reverse / CCCTCCACTGGATTCAATGACTGA

Supplementary Table S2.Sequences of amplification, extension primers and TaqMan probes for ADH1B and ADH1C SNP genotyping

Gene / Locus / Sequence (5'-3')
ADH1B / c.-992C>G / Forward / GCATTAACTGACCCCTGTGATAGG
Reverse / ACACGCAGGAAACCAAGTCTAC
Probe-1 (VIC) / CAGCCAGGCTGATAT
Probe-2 (FAM) / CAGCCAGGGTGATAT
c.-957C>A / Forward / GGCTGATATGTAGACTTGGTTTCCT
Reverse / GCACCCTCCTAAATGTTAGCTACAATT
Probe-1 (VIC) / CAGTTTATAGGGAAAAGA
Probe-2 (FAM) / ACAGTTTATAGTGAAAAGA
c.143A>G (p.His47Arg) / Forward / TCTTTTCTGAATCTGAACAGCTTCTCTTT
Reverse / GGTCACCAGGTTGCCACTAAC
Probe-1 (VIC) / CTGTAGGAATCTGTCGCACA
Probe-2 (FAM) / CTGTAGGAATCTGTCACACA
c.259+91G>T / Forward / CACCATCCAGGATTTCCCAG
Reverse / TGGGTCAGGCAGGCAGA
Extension / GCTTGCACAAGGTCACCGCGCAG
c.259+205G>A / Forward / GGTCACCGCGCAGCC
Reverse / GCCACACTGGTAGGCACTGT
Extension / ATTCAGGCACTCATGTATTGTACCCTTCAACC
c.259+790T>C / Forward / AGTAAGGCTGTCATTTTGTTGTTAAT
Reverse / TTAATAACTAGGACATTGCCATACCATC
Extension / ATCACCTATGCTATACATTCCACATGATCAAGAAAAATTGTTGGT
c.1180A>G / Forward / CCTTCCCCTGTAGCAGTCTTC
Reverse / GCTGAATTAATGATATTTCCTAGCTGTTGCT
Probe-1 (VIC) / CTACCCTACAAGATCTG
Probe-2 (FAM) / CCCTACGAGATCTG
ADH1C / c.-1064C>T / Forward / CTCTGTAACACTCATGGCAAAGGT
Reverse / GTTCCTCCTGGTGGGTTCATG
Probe-1 (VIC) / CTGGCTTCAGGAGTGA
Probe-2 (FAM) / CTGGCTTCAAGAGTGA
c.-325G>C / Forward / ACCATGTGGATTCAAAATTGCA
Reverse / GAAATGGGCACCGAACCAG
Extension / CTCTCTTCCTCCTCTCTCAA
c.120+46T>G / Forward / CATCTCTCTGTCTGATGTTCTAGGTAAT
Reverse / CCTTCATATACTGTTTGCCCACTT
Extension / TTAAGGTGAAATACTTTTTCCATTTGTATTTAATTTTAAGTTTAAAGAAT
c.121-252G>A / Forward / AGTTCACAATCAATTTGCATTAGATATT
Reverse / TCTCAAGGGTTTTGCTGATAATC
Extension / TGCAATTTCTACTTCTTTTTCTTTTAACTATGCATTCTTCTATATTAT
c.259+32A>G / Forward / ACCAGGTACAGGATTCACACTCA
Reverse / ATCCAGGCTGACTAATCTTGTGATG
Probe-1 (VIC) / AACATGCCTTAGTTCAC
Probe-2 (FAM) / CATGCCTTGGTTCAC
c.312T>C (p.Cys104) / Forward / GCAGGTGATAAAGTCATCCCG
Reverse / GGATTGCCTAGACTGGGCAG
Extension / AATGATGATACTCCTCAGTGTGGAAAATGCAGAATTTG
c.453A>G (p.Thr151) / Forward / GGAAGCCCATCCACCACTT
Reverse / CGAAAATCCACAGCCAATGAG
Extension / GATGGCCACTGCATTCTCATCCACCAC
c.474G>A (p.Val158) / Forward / CCCAGTCTAGGCAATCCTCG
Reverse / AAGAAATTGCTTCCCTTTTGG
Extension / GTGGTGGATGAGAATGCAGT
c.815G>A (p.Arg272Gln) / Forward / CCCTCAAGACTACAAGAAACCCA
Reverse / CCATTCATCATTAAAAATATCCTTTATACA
Extension / TGTGGATTTTTCGTTTGAAGTCATCGGTC
c.1148A>G (p.Ile349Val) / Forward / TTAAGAGTAAAGAATCTGTCCCCAAAC
Reverse / TAATTTTTTCTCATCCTTCCACCTT
Extension / GGCTAAGAAGTTTTCACTGGATGCATTAATAACAAAT

Supplementary Figure S1. Sequence chromatograms of 14 polymorphisms in ADH1B and ADH1C genes. Red boxes indicate polymorphic sites.