Supplementary Material

Identification of magnetic nanoparticles for combined positioning and lentiviral transduction of endothelial cells

Daniela Wenzel, Sarah Rieck, Sarah Vosen, *Olga Mykhaylyk, #Christina Trueck, +Dietmar Eberbeck, +Lutz Trahms, #Katrin Zimmermann, #Alexander Pfeifer, Bernd K. Fleischmann

Institute of Physiology I, University of Bonn, *Institute of Experimental Oncology, TU München, #Institute of Pharmacology and Toxicology, University of Bonn, +Physikalisch-Technische Bundesanstalt, Berlin

MNP / Iron content [g Fe/g dry weight] / Coating content [g coating material/g dry weight] / Hydrated particle diameter [nm] / Zeta potential [mV] / Coating composition / Reference
1 / Chi-Mag1 / 0.52 / 0.28 / 1200* / + 21,5 / chitosan oligosaccaride lactate / -
2 / DHDP-Mag1 / 0.44 / 0.39 / n.d. / - 39,0 / dihexadecyl phosphate / -
3 / NDT-Mag1 / 0.679 / 0.06 / 92 ± 49* / + 34,3 / 61 mass%1,9-nonanedithiol/39 mass%FSA† / (1)
4 / NDT-Mag2 / 0.5 / 0.31 / 68 ± 30* / - 14,6 / NDT /FSE / -
5 / PEI-Mag2 / 0.56 / 0.23 / 31,1 ± 14,3 / 55.0 ± 0.7 / 32% (w/w) PEI /68% (w/w) FSA† / (2, 3)
6 / PL-Mag1 / 0.41 / 0.43 / 106 ± 40* / -13.3 ± 1.6 / Pluronic-127/FSE / -
7 / PL-Mag2 / 0.21 / 0.71 / 101 ± 20* / - 18,3 ± 0.2 / 100 mass% FSA† / (2, 3)
8 / SO-Mag2 / n.d. / n.d. / 427 ± 90* / 37.4 ± 1.6 / SiOx /Phosphonate-PEI / (4, 5)
9 / Tween-60-Mag1 / 0.43 / 0.41 / n.d. / - 1,4 / Tween60/FSA / -
10 / Tween80-Mag1 / 0.42 / 0.42 / 53 ± 27* / + 27,8 / Tween80 / -

*assembly of particles

†according to the X-ray photoelectron spectroscopy data.

SiOx, Silicon Oxide; Phosphonate, phosphonate groups of the product of hydrolization and condensation of 3-(Trihydroxysilyl)propylmethyl-phosphonate; fluorinated surfactant ZONYL FSA (lithium 3-[2-(perfluoroalkyl)ethylthio]propionate); PEI, 25-kDa branched polyethylenimine; (ZONYL)-FSA, Lithium 3-[2-(perfluoroalkyl)ethylthio]propionate; SiOx, Silicon Oxide; Phosphonate, phosphonate groups of the product of hydrolization and condensation of 3-(Trihydroxysilyl)propylmethyl-phosphonate; (ZONYL) FSE, anionic phosphate fluorosurfactant; NDT, 1,9-nonanedithiol;

Tween60, Polyethylene glycol sorbitan monostearate; Tween80, Polyoxyethylene Sorbitan Monooleate;

Suppl. Figure 1: Table of the magnetic nanoparticles (MNPs) tested in magnetic rack experiments. The table shows iron content, coating content, hydrated particle diameter, zeta potential and the composition of coating of 10 types of MNPs.

Suppl. Figure 2: LDH assay of MNP-assisted lentiviral transduction. LDH assay of bPAECs transduced with LV/NDT (0.04), LV/PEI (0.04) or pre-loaded with NDT (25) and transduced with PEI (0.04), cell lysis served as positive control.

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