LOCUS pMA2867=knockdow 7857 bp DNA circular 26-NOV-2008
DEFINITION pLenti-Tet-mCherry-mir155-SV40-puro.
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/
COMMENT VNTDATE|499617744|
COMMENT VNTDBDATE|499968278|
COMMENT LSOWNER|
COMMENT VNTNAME|pMA2867=knockdown|
COMMENT VNTAUTHORNAME|Demo User|
FEATURES Location/Qualifiers
misc_binding 2867..2885
/vntifkey="20"
/label=TetO
misc_binding 2831..2849
/vntifkey="20"
/label=TetO
misc_binding 2796..2814
/vntifkey="20"
/label=TetO
misc_binding 2760..2778
/vntifkey="20"
/label=TetO
misc_binding 2724..2742
/vntifkey="20"
/label=TetO
misc_binding 2689..2707
/vntifkey="20"
/label=TetO
misc_binding 2653..2671
/vntifkey="20"
/label=TetO
promoter 2899..2958
/vntifkey="29"
/label=pCMVmin
LTR 5395..6084
/vntifkey="19"
/label=LTR
/note="3'LTR"
CDS complement(6867..7724)
/vntifkey="4"
/label=Ap-res
LTR 5543..5997
/vntifkey="19"
/label=LTR\proviral
LTR 847..1521
/vntifkey="19"
/label=LTR
/note="5'LTR"
misc_feature 1551..2310
/vntifkey="21"
/label=HIV\RRE
promoter 4293..4633
/vntifkey="29"
/label=SV40\promoter-enhancer
CDS 4646..5245
/vntifkey="4"
/label=PAC\(puro-res)
misc_feature 3746..3772
/vntifkey="21"
/label=5'\miR-155\flank
CDS 2976..3683
/vntifkey="4"
/label=mCherry\hum
misc_feature 4199..4243
/vntifkey="21"
/label=3'\miR-155\flank
misc_feature 4199..4202
/vntifkey="21"
/label=3'\overhang
CDS 4009..4188
/vntifkey="4"
/label=lacZalpha
BASE COUNT 2053 a 1993 c 2108 g 1703 t
ORIGIN
1 gtcgatcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
61 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
121 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
181 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgc
241 gcgttgacat tgattattga ctagctagtt attaatagta atcaattacg gggtcattag
301 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
361 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
421 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
481 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
541 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
601 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
661 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
721 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
781 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tagaccagat ctgagcctgg
841 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
901 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
961 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
1021 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
1081 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
1141 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
1201 ttcggttaag gccaggggga aagaaaaaat ataaataaac atatagtatg ggcaagcagg
1261 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
1321 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
1381 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
1441 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
1501 ggccgcgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
1561 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
1621 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
1681 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
1741 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
1801 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
1861 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
1921 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
1981 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
2041 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
2101 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
2161 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
2221 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
2281 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
2341 cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattcgac aaatggcagt
2401 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
2461 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
2521 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag taccgggccg
2581 gcccgctcta gctagacgta ttaccgccag atcccccatc aagcttatcg ataccgtcga
2641 cctcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
2701 atagagaacg atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact
2761 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat
2821 gtcgagttta tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
2881 agagaacgta tgtcgaggta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt
2941 agtgaaccgt cagatcgcct ggagaattcg ccaccatggt gagcaagggc gaggaggaca
3001 acatggccat catcaaggag ttcatgcggt tcaaggtgca catggagggc agcgtgaacg
3061 gccacgagtt cgagatcgag ggcgagggcg agggccggcc ctacgagggc acccagaccg
3121 ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgagccccc
3181 agttcatgta cggcagcaag gcctacgtga agcaccccgc cgacatcccc gactacctga
3241 agctgagctt ccccgagggc ttcaagtggg agcgggtgat gaacttcgag gacggcggcg
3301 tggtgaccgt gacccaggac agcagcctcc aggacggcga gttcatctac aaggtgaagc
3361 tgcggggcac caacttcccc agcgacggcc ccgtgatgca gaagaagaca atgggctggg
3421 aggccagcag cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcagc
3481 ggctgaagct gaaggacggc ggccactacg acgccgaggt gaagaccacc tacaaggcca
3541 agaagcccgt gcagctcccc ggcgcctaca acgtgaacat caagctggac atcaccagcc
3601 acaacgagga ctacaccatc gtggagcagt acgagcgggc cgagggccgg cacagcaccg
3661 gcggcatgga cgagctgtac aagtaagcta agcacttcgt ggccgtcgat cgtttaaagg
3721 gaggtagtga gtcgaccagt ggatcctgga ggcttgctga aggctgtatg ctgatcgcag
3781 gtcgcgggac gtcgcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
3841 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
3901 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
3961 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
4021 acggattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
4081 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
4141 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcac ctgcgatcca
4201 ggacacaagg cctgttacta gcactcacat ggaacaaatg gcccagatcc cccgggctgc
4261 aggaattgat ggccgccacc gcggtggagg accctgtgga atgtgtgtca gttagggtgt
4321 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
4381 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
4441 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
4501 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
4561 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
4621 ggcttttgca aaaagctagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
4681 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
4741 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
4801 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
4861 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
4921 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
4981 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
5041 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
5101 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
5161 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
5221 catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag
5281 gagcgcacga ccccatgcat cgatcaattc gatgggggat caattcgatt aagtcgaggg
5341 tcgatcgacc tcgatcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca
5401 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
5461 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
5521 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc
5581 cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca
5641 gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
5701 caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc
5761 ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta
5821 gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac
5881 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
5941 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
6001 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatgtg agcaaaaggc
6061 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
6121 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
6181 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
6241 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
6301 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
6361 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
6421 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
6481 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
6541 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
6601 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
6661 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
6721 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
6781 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
6841 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
6901 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
6961 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
7021 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
7081 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
7141 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
7201 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
7261 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
7321 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
7381 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
7441 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
7501 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
7561 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
7621 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
7681 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
7741 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
7801 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgac
//