Anova Supplementarydata

Anova supplementarydata:

Table for heavy metal parameters :

Anova table for Lead (Pb) :

Source / DF / Sum of squares / Mean squares / F / Pr > F
PLACE / 1 / 481.586 / 481.586 / 3083.505 / < 0.0001
SAMPLE / 2 / 52.975 / 26.488 / 169.596 / < 0.0001
WEIGHT / 1 / 75.768 / 75.768 / 485.129 / < 0.0001
TISSUE / 1 / 123.167 / 123.167 / 788.613 / < 0.0001
PLACE*SAMPLE / 2 / 38.717 / 19.358 / 123.948 / < 0.0001
PLACE*WEIGHT / 1 / 33.374 / 33.374 / 213.691 / < 0.0001
PLACE*TISSUE / 1 / 63.563 / 63.563 / 406.981 / < 0.0001
SAMPLE*WEIGHT / 2 / 10.214 / 5.107 / 32.700 / < 0.0001
SAMPLE*TISSUE / 2 / 3.090 / 1.545 / 9.892 / 0.000
WEIGHT*TISSUE / 1 / 3.781 / 3.781 / 24.211 / < 0.0001
PLACE*SAMPLE*WEIGHT / 2 / 6.934 / 3.467 / 22.198 / < 0.0001
PLACE*SAMPLE*TISSUE / 2 / 4.778 / 2.389 / 15.297 / < 0.0001
PLACE*WEIGHT*TISSUE / 1 / 0.072 / 0.072 / 0.462 / 0.500
SAMPLE*WEIGHT*TISSUE / 2 / 2.791 / 1.395 / 8.934 / 0.001
PLACE*SAMPLE*WEIGHT*TISSUE / 2 / 1.160 / 0.580 / 3.713 / 0.032

Anova table for Copper (Cu) :

Source / DF / Sum of squares / Mean squares / F / Pr > F
PLACE / 1 / 391.230 / 391.230 / 787.021 / < 0.0001
SAMPLE / 2 / 81.916 / 40.958 / 82.394 / < 0.0001
WEIGHT / 1 / 116.319 / 116.319 / 233.994 / < 0.0001
TISSUE / 1 / 47.849 / 47.849 / 96.255 / < 0.0001
PLACE*SAMPLE / 2 / 82.914 / 41.457 / 83.397 / < 0.0001
PLACE*WEIGHT / 1 / 61.466 / 61.466 / 123.649 / < 0.0001
PLACE*TISSUE / 1 / 6.579 / 6.579 / 13.235 / 0.001
SAMPLE*WEIGHT / 2 / 21.768 / 10.884 / 21.895 / < 0.0001
SAMPLE*TISSUE / 2 / 18.998 / 9.499 / 19.109 / < 0.0001
WEIGHT*TISSUE / 1 / 11.944 / 11.944 / 24.027 / < 0.0001
PLACE*SAMPLE*WEIGHT / 2 / 29.804 / 14.902 / 29.977 / < 0.0001
PLACE*SAMPLE*TISSUE / 2 / 20.073 / 10.036 / 20.190 / < 0.0001
PLACE*WEIGHT*TISSUE / 1 / 0.261 / 0.261 / 0.525 / 0.472
SAMPLE*WEIGHT*TISSUE / 2 / 3.695 / 1.847 / 3.716 / 0.032
PLACE*SAMPLE*WEIGHT*TISSUE / 2 / 9.186 / 4.593 / 9.239 / 0.000

Anova table for Cadmium (Cd) :

Source / DF / Sum of squares / Mean squares / F / Pr > F
PLACE / 1 / 36.466 / 36.466 / 311.712 / < 0.0001
SAMPLE / 2 / 0.725 / 0.362 / 3.097 / 0.054
WEIGHT / 1 / 2.832 / 2.832 / 24.210 / < 0.0001
TISSUE / 1 / 1.575 / 1.575 / 13.466 / 0.001
PLACE*SAMPLE / 2 / 1.255 / 0.628 / 5.365 / 0.008
PLACE*WEIGHT / 1 / 1.015 / 1.015 / 8.679 / 0.005
PLACE*TISSUE / 1 / 0.336 / 0.336 / 2.874 / 0.097
SAMPLE*WEIGHT / 2 / 0.959 / 0.479 / 4.098 / 0.023
SAMPLE*TISSUE / 2 / 0.819 / 0.410 / 3.501 / 0.038
WEIGHT*TISSUE / 1 / 0.006 / 0.006 / 0.052 / 0.821
PLACE*SAMPLE*WEIGHT / 2 / 0.614 / 0.307 / 2.626 / 0.083
PLACE*SAMPLE*TISSUE / 2 / 0.556 / 0.278 / 2.376 / 0.104
PLACE*WEIGHT*TISSUE / 1 / 0.567 / 0.567 / 4.848 / 0.033
SAMPLE*WEIGHT*TISSUE / 2 / 0.971 / 0.485 / 4.149 / 0.022
PLACE*SAMPLE*WEIGHT*TISSUE / 2 / 0.243 / 0.122 / 1.039 / 0.362

Anova table for Nickel (Ni):

Source / DF / Sum of squares / Mean squares / F / Pr > F
PLACE / 1 / 56.818 / 56.818 / 1043.526 / < 0.0001
SAMPLE / 2 / 2.033 / 1.016 / 18.665 / < 0.0001
WEIGHT / 1 / 7.488 / 7.488 / 137.534 / < 0.0001
TISSUE / 1 / 0.080 / 0.080 / 1.469 / 0.231
PLACE*SAMPLE / 2 / 2.024 / 1.012 / 18.588 / < 0.0001
PLACE*WEIGHT / 1 / 7.220 / 7.220 / 132.604 / < 0.0001
PLACE*TISSUE / 1 / 0.186 / 0.186 / 3.417 / 0.071
SAMPLE*WEIGHT / 2 / 1.733 / 0.866 / 15.914 / < 0.0001
SAMPLE*TISSUE / 2 / 0.903 / 0.452 / 8.293 / 0.001
WEIGHT*TISSUE / 1 / 0.353 / 0.353 / 6.480 / 0.014
PLACE*SAMPLE*WEIGHT / 2 / 1.563 / 0.781 / 14.349 / < 0.0001
PLACE*SAMPLE*TISSUE / 2 / 0.698 / 0.349 / 6.413 / 0.003
PLACE*WEIGHT*TISSUE / 1 / 0.414 / 0.414 / 7.605 / 0.008
SAMPLE*WEIGHT*TISSUE / 2 / 0.421 / 0.211 / 3.867 / 0.028
PLACE*SAMPLE*WEIGHT*TISSUE / 2 / 0.416 / 0.208 / 3.816 / 0.029

Anova table for Zinc (Zn) :

Source / DF / Sum of squares / Mean squares / F / Pr > F
PLACE / 1 / 1620.086 / 1620.086 / 912.717 / < 0.0001
SAMPLE / 2 / 475.804 / 237.902 / 134.028 / < 0.0001
WEIGHT / 1 / 75.184 / 75.184 / 42.357 / < 0.0001
TISSUE / 1 / 18.773 / 18.773 / 10.576 / 0.002
PLACE*SAMPLE / 2 / 485.137 / 242.568 / 136.657 / < 0.0001
PLACE*WEIGHT / 1 / 76.354 / 76.354 / 43.016 / < 0.0001
PLACE*TISSUE / 1 / 27.547 / 27.547 / 15.519 / 0.000
SAMPLE*WEIGHT / 2 / 125.032 / 62.516 / 35.220 / < 0.0001
SAMPLE*TISSUE / 2 / 37.689 / 18.844 / 10.616 / 0.000
WEIGHT*TISSUE / 1 / 4.953 / 4.953 / 2.791 / 0.101
PLACE*SAMPLE*WEIGHT / 2 / 94.124 / 47.062 / 26.514 / < 0.0001
PLACE*SAMPLE*TISSUE / 2 / 39.510 / 19.755 / 11.129 / 0.000
PLACE*WEIGHT*TISSUE / 1 / 7.116 / 7.116 / 4.009 / 0.051
SAMPLE*WEIGHT*TISSUE / 2 / 6.361 / 3.180 / 1.792 / 0.178
PLACE*SAMPLE*WEIGHT*TISSUE / 2 / 8.980 / 4.490 / 2.530 / 0.090

Table for antioxidant parameters :

Anova table for Superoxide Dismutase (SOD) :

Source / DF / Sum of squares / Mean squares / F / Pr > F
PLACE / 1 / 199.001 / 199.001 / 88.108 / < 0.0001
FISH / 2 / 0.889 / 0.444 / 0.197 / 0.822
WEIGHT / 1 / 845.290 / 845.290 / 374.252 / < 0.0001
TISSUE / 1 / 1291.167 / 1291.167 / 571.664 / < 0.0001
PLACE*FISH / 2 / 7.776 / 3.888 / 1.721 / 0.190
PLACE*WEIGHT / 1 / 0.190 / 0.190 / 0.084 / 0.773
PLACE*TISSUE / 1 / 112.250 / 112.250 / 49.699 / < 0.0001
FISH*WEIGHT / 2 / 8.172 / 4.086 / 1.809 / 0.175
FISH*TISSUE / 2 / 38.975 / 19.488 / 8.628 / 0.001
WEIGHT*TISSUE / 1 / 0.661 / 0.661 / 0.293 / 0.591
PLACE*FISH*WEIGHT / 2 / 0.580 / 0.290 / 0.128 / 0.880
PLACE*FISH*TISSUE / 2 / 1.230 / 0.615 / 0.272 / 0.763
PLACE*WEIGHT*TISSUE / 1 / 0.281 / 0.281 / 0.125 / 0.726
FISH*WEIGHT*TISSUE / 2 / 0.107 / 0.054 / 0.024 / 0.976
PLACE*FISH*WEIGHT*TISSUE / 2 / 0.426 / 0.213 / 0.094 / 0.910

Anova table for Catalase (CAT) :

Source / DF / Sum of squares / Mean squares / F / Pr > F
PLACE / 1 / 168.056 / 168.056 / 665.201 / < 0.0001
FISH / 2 / 0.724 / 0.362 / 1.434 / 0.248
WEIGHT / 1 / 97.534 / 97.534 / 386.060 / < 0.0001
TISSUE / 1 / 27.380 / 27.380 / 108.376 / < 0.0001
PLACE*FISH / 2 / 1.391 / 0.696 / 2.753 / 0.074
PLACE*WEIGHT / 1 / 0.245 / 0.245 / 0.970 / 0.330
PLACE*TISSUE / 1 / 0.020 / 0.020 / 0.079 / 0.780
FISH*WEIGHT / 2 / 1.501 / 0.751 / 2.971 / 0.061
FISH*TISSUE / 2 / 7.093 / 3.547 / 14.038 / < 0.0001
WEIGHT*TISSUE / 1 / 0.467 / 0.467 / 1.849 / 0.180
PLACE*FISH*WEIGHT / 2 / 0.023 / 0.012 / 0.046 / 0.955
PLACE*FISH*TISSUE / 2 / 1.613 / 0.807 / 3.193 / 0.050
PLACE*WEIGHT*TISSUE / 1 / 0.005 / 0.005 / 0.020 / 0.889
FISH*WEIGHT*TISSUE / 2 / 1.668 / 0.834 / 3.301 / 0.045
PLACE*FISH*WEIGHT*TISSUE / 2 / 0.310 / 0.155 / 0.614 / 0.546

Anova table for Glutahione Peroxidase (GPx) :

Source / DF / Sum of squares / Mean squares / F / Pr > F
PLACE / 1 / 126.140 / 126.140 / 678.274 / < 0.0001
FISH / 2 / 4.995 / 2.498 / 13.430 / < 0.0001
WEIGHT / 1 / 88.667 / 88.667 / 476.774 / < 0.0001
TISSUE / 1 / 48.840 / 48.840 / 262.621 / < 0.0001
PLACE*FISH / 2 / 0.035 / 0.018 / 0.095 / 0.910
PLACE*WEIGHT / 1 / 0.420 / 0.420 / 2.259 / 0.139
PLACE*TISSUE / 1 / 0.170 / 0.170 / 0.915 / 0.344
FISH*WEIGHT / 2 / 3.300 / 1.650 / 8.873 / 0.001
FISH*TISSUE / 2 / 0.112 / 0.056 / 0.301 / 0.741
WEIGHT*TISSUE / 1 / 0.420 / 0.420 / 2.259 / 0.139
PLACE*FISH*WEIGHT / 2 / 0.174 / 0.087 / 0.467 / 0.630
PLACE*FISH*TISSUE / 2 / 0.092 / 0.046 / 0.247 / 0.782
PLACE*WEIGHT*TISSUE / 1 / 0.233 / 0.233 / 1.255 / 0.268
FISH*WEIGHT*TISSUE / 2 / 0.174 / 0.087 / 0.467 / 0.630
PLACE*FISH*WEIGHT*TISSUE / 2 / 0.034 / 0.017 / 0.090 / 0.914

Anova table for Glutathione S-Transferase (GST) :

Source / DF / Sum of squares / Mean squares / F / Pr > F
PLACE / 1 / 92.934 / 92.934 / 145.906 / < 0.0001
FISH / 2 / 3.541 / 1.771 / 2.780 / 0.072
WEIGHT / 1 / 98.000 / 98.000 / 153.860 / < 0.0001
TISSUE / 1 / 37.556 / 37.556 / 58.962 / < 0.0001
PLACE*FISH / 2 / 3.321 / 1.661 / 2.607 / 0.084
PLACE*WEIGHT / 1 / 0.009 / 0.009 / 0.014 / 0.906
PLACE*TISSUE / 1 / 0.056 / 0.056 / 0.087 / 0.769
FISH*WEIGHT / 2 / 4.000 / 2.000 / 3.140 / 0.052
FISH*TISSUE / 2 / 3.498 / 1.749 / 2.746 / 0.074
WEIGHT*TISSUE / 1 / 0.027 / 0.027 / 0.043 / 0.837
PLACE*FISH*WEIGHT / 2 / 0.018 / 0.009 / 0.014 / 0.986
PLACE*FISH*TISSUE / 2 / 1.778 / 0.889 / 1.396 / 0.258
PLACE*WEIGHT*TISSUE / 1 / 0.005 / 0.005 / 0.008 / 0.930
FISH*WEIGHT*TISSUE / 2 / 0.054 / 0.027 / 0.043 / 0.958
PLACE*FISH*WEIGHT*TISSUE / 2 / 0.010 / 0.005 / 0.008 / 0.992

Anova table for Glutathione Reductase (GRd) :

Source / DF / Sum of squares / Mean squares / F / Pr > F
PLACE / 1 / 115.014 / 115.014 / 481.174 / < 0.0001
FISH / 2 / 4.090 / 2.045 / 8.555 / 0.001
WEIGHT / 1 / 98.936 / 98.936 / 413.908 / < 0.0001
TISSUE / 1 / 31.205 / 31.205 / 130.550 / < 0.0001
PLACE*FISH / 2 / 0.514 / 0.257 / 1.076 / 0.349
PLACE*WEIGHT / 1 / 0.000 / 0.000 / 0.000 / 1.000
PLACE*TISSUE / 1 / 0.934 / 0.934 / 3.907 / 0.054
FISH*WEIGHT / 2 / 4.271 / 2.136 / 8.934 / 0.001
FISH*TISSUE / 2 / 0.443 / 0.222 / 0.927 / 0.403
WEIGHT*TISSUE / 1 / 0.002 / 0.002 / 0.009 / 0.924
PLACE*FISH*WEIGHT / 2 / 0.000 / 0.000 / 0.000 / 1.000
PLACE*FISH*TISSUE / 2 / 0.988 / 0.494 / 2.066 / 0.138
PLACE*WEIGHT*TISSUE / 1 / 0.000 / 0.000 / 0.000 / 1.000
FISH*WEIGHT*TISSUE / 2 / 0.004 / 0.002 / 0.009 / 0.991
PLACE*FISH*WEIGHT*TISSUE / 2 / 0.000 / 0.000 / 0.000 / 1.000

Anova table for GSH :

Source / DF / Sum of squares / Mean squares / F / Pr > F
PLACE / 1 / 79.590 / 79.590 / 116.853 / < 0.0001
FISH / 2 / 0.085 / 0.043 / 0.063 / 0.939
WEIGHT / 1 / 144.783 / 144.783 / 212.570 / < 0.0001
TISSUE / 1 / 0.133 / 0.133 / 0.196 / 0.660
PLACE*FISH / 2 / 0.512 / 0.256 / 0.376 / 0.689
PLACE*WEIGHT / 1 / 0.003 / 0.003 / 0.005 / 0.943
PLACE*TISSUE / 1 / 30.031 / 30.031 / 44.092 / < 0.0001
FISH*WEIGHT / 2 / 1.022 / 0.511 / 0.750 / 0.478
FISH*TISSUE / 2 / 0.145 / 0.073 / 0.107 / 0.899
WEIGHT*TISSUE / 1 / 0.483 / 0.483 / 0.710 / 0.404
PLACE*FISH*WEIGHT / 2 / 0.002 / 0.001 / 0.001 / 0.999
PLACE*FISH*TISSUE / 2 / 3.161 / 1.580 / 2.320 / 0.109
PLACE*WEIGHT*TISSUE / 1 / 0.003 / 0.003 / 0.005 / 0.943
FISH*WEIGHT*TISSUE / 2 / 1.022 / 0.511 / 0.750 / 0.478
PLACE*FISH*WEIGHT*TISSUE / 2 / 0.002 / 0.001 / 0.001 / 0.999

Anova table for Malonaldealdehyde (MDA) :

Source / DF / Sum of squares / Mean squares / F / Pr > F
PLACE / 1 / 160.503 / 160.503 / 643.802 / < 0.0001
FISH / 2 / 2.230 / 1.115 / 4.473 / 0.017
WEIGHT / 1 / 117.811 / 117.811 / 472.558 / < 0.0001
TISSUE / 1 / 20.161 / 20.161 / 80.870 / < 0.0001
PLACE*FISH / 2 / 2.400 / 1.200 / 4.814 / 0.012
PLACE*WEIGHT / 1 / 0.517 / 0.517 / 2.073 / 0.156
PLACE*TISSUE / 1 / 4.550 / 4.550 / 18.251 / < 0.0001
FISH*WEIGHT / 2 / 1.823 / 0.911 / 3.655 / 0.033
FISH*TISSUE / 2 / 1.803 / 0.901 / 3.615 / 0.034
WEIGHT*TISSUE / 1 / 0.700 / 0.700 / 2.808 / 0.100
PLACE*FISH*WEIGHT / 2 / 0.317 / 0.158 / 0.636 / 0.534
PLACE*FISH*TISSUE / 2 / 0.050 / 0.025 / 0.101 / 0.904
PLACE*WEIGHT*TISSUE / 1 / 0.361 / 0.361 / 1.449 / 0.235
FISH*WEIGHT*TISSUE / 2 / 0.184 / 0.092 / 0.368 / 0.694
PLACE*FISH*WEIGHT*TISSUE / 2 / 0.023 / 0.011 / 0.045 / 0.956