Supplementary Table 3. Primer sequence, melting temperature (Tm), repeat motif, and cross-amplification result of each microsatellite primer pair used in the cross-amplification tests between pearl millet (Pennisetum glaucum) and napiergrass (P. purpureum Schum.).

Marker name / Forward primer (5' to 3') / Reverse primer (5' to 3') / Tm (oC) / Repeat motif / Reference / Cross amplification / M/P
ICMP3001′ / GCCGTCGACATTAACCAACT / AAACATAGGGCTTGGCACTG / 60.4 / (AAAC)4 / Senthilvel et al., 2008 / Yes / M
ICMP3002′ / CGAGCCGCCATAGTTGAC / TACACACACATTGCCACACG / 61.3 / (AAG)7 / Senthilvel et al., 2008 / Yes* / P
ICMP3004′ / TGTTACGCAGTGCTCGGTAG / ATATAGGGGCGCGCAATAGT / 61.4 / (AC)14 / Senthilvel et al., 2008 / Yes / P
ICMP3014′ / TGCTTCACAGCCTCTCCATA / CCACCATGCAACAGCAATAA / 59.4 / (ACC)8 / Senthilvel et al., 2008 / Yes* / P
ICMP3016′ / GTCAACCATTTGGGCTCACT / GGGAGAAATGTGGGGAGAGA / 61.4 / (CA)17 / Senthilvel et al., 2008 / No / -
ICMP3017′ / CACCAAACAGCATCAAGCAG / AGGTAGCCGAGGAAGGTGAG / 62.5 / (CAG)7 / Senthilvel et al., 2008 / Yes / P
ICMP3018′ / ACGAGGACAAGCTCTTGGAA / ACGGCGCATACTCGATCATA / 60.4 / (CATG)4 / Senthilvel et al., 2008 / Yes / P
ICMP3019′ / GCGCACCACCTGTGTCTAT / CATGCAGAGAAAAATCAAGCA / 59.5 / (CGTA)4 / Senthilvel et al., 2008 / Yes / P
ICMP3020′ / GTTCCATGGAGCTGGAAGTC / GCTAGAACAGGGCCGTTACA / 62.4 / (CGTG)5 / Senthilvel et al., 2008 / Yes / P
ICMP3021′ / GCCGACAGGAAGATTACGAT / AGCAAAACGCAGAACAACAG / 59.4 / (CGTG)5 / Senthilvel et al., 2008 / Yes / P
ICMP3023′ / GTTCCATGGAGCTGGAAGTC / GCTAGAACAGGGCCGTTACA / 62.4 / (CGTG)5 / Senthilvel et al., 2008 / Yes / M
ICMP3024′ / ATCGAGGCCAAGTACGTGAT / CGAGCTTCTAGCTCCAATCC / 61.4 / (CT)11(CT)5 / Senthilvel et al., 2008 / No / -
ICMP3026′ / GTGAGGCCTCGAACAAACAC / GCCGACCAAGAACTTCATACA / 61.5 / (CTC)6 / Senthilvel et al., 2008 / Yes / P
ICMP3027′ / ACACCATCACCGACAACAAA / AGTGACCTGGGGTACAGACG / 61.5 / (GAT)6 / Senthilvel et al., 2008 / Yes / P
ICMP3028′ / ACGATTCTTCGTCGTTCCAG / GATACGCGCGAGCTACATTT / 60.4 / (GATC)4 / Senthilvel et al., 2008 / Yes* / P
ICMP3032′ / AGGTAGCCGAGGAAGGTGAG / CAACAGCATCAAGCAGGAGA / 62.5 / (GCT)8(ACAT)3 / Senthilvel et al., 2008 / Yes / P
ICMP3033′ / GAGGGCCAGCTCTCCTAGAT / CCCTAACCACAGAGGGACAC / 64.5 / TGCC(4) / Senthilvel et al., 2008 / Yes / P
ICMP3037’ / CGCTGCGTTTATTGAAGGAG / GGCGAAACAAAGAGAGTTGG / 60.4 / (TGGA)4(GT)5 / Senthilvel et al., 2008 / No / -
ICMP3039′ / GGCACGAGGGGCTAAGTAA / GGAACGCCGAGTACACAGAT / 62.4 / (TGT)6 / Senthilvel et al., 2008 / No / -
ICMP3041′ / GCATGCTTGCCAACTTTGTA / TACCCCAGTGAACACCGTTT / 59.4 / (TTCT)4 / Senthilvel et al., 2008 / No / -
ICMP3045′ / ACAAGGACGACAAGGACCAC / CCTCTCCAAGCACATGTTTC / 61.4 / (AAG)5 / Senthilvel et al., 2008 / Yes* / P
ICMP3046′ / CGTGACCCTGTGGTACTGTG / CGCGCAAATTTGTGGGTA / 61.1 / (CA)9 / Senthilvel et al., 2008 / No / -
ICMP3049′ / GAGCTGAACACGCTCAAGG / CAGATGACATCCATCCGTTG / 61.4 / (CCTG)4(TGCCC)3 / Senthilvel et al., 2008 / Yes / M
ICMP3050′ / ATGTCCAGTGTTGACGGTGA / CGGGGAAGAGACAGGCTACT / 62.4 / (TA)8 / Senthilvel et al., 2008 / Yes / P
ICMP3051′ / TCTTCTTCCGCATCCTCTGT / GTACCGCCCTTTGTGTTGAT / 60.4 / (CTAGA)3 / Senthilvel et al., 2008 / No / -
ICMP3052′ / GCCAGATGGTTAAGCTTCCA / GGCGAGAATATGATGGGTGA / 60.4 / (AC)8(AC)5 / Senthilvel et al., 2008 / No / -
ICMP3054′ / AGGATCGGCACGAGGATAG / GCTCTTGAAGGAGACGCTGT / 62.4 / (CCA)5 / Senthilvel et al., 2008 / No / -
ICMP3055′ / CCCAAACGCAAGTAGGGTTA / CCTTCTCCTGCCCCAGAC / 62.5 / (CCG)5 / Senthilvel et al., 2008 / Yes / P
ICMP3056′ / ACGGAGCTACGGTTGGAATA / CACAAGGGACCCCACGATA / 61.4 / (TGG)5 / Senthilvel et al., 2008 / Yes* / P
ICMP3063′ / TCCGGTAGAGACCGTAATGG / GGCACTCCCTAGCAAAATGA / 61.4 / (GTG)5 / Senthilvel et al., 2008 / Yes / M
ICMP3064′ / AGGCTCATCCCAAGTTCTCA / CTGTTGGCTCACATTGGATG / 60.4 / (TCTCT)3 / Senthilvel et al., 2008 / No / -
ICMP3066′ / GGCCCCAAGTAACTTCCCTA / TGTCAGACACAGATGCCACA / 61.4 / (AG)7 / Senthilvel et al., 2008 / Yes* / P
ICMP3068′ / CTGGCAAAGTTGTAGCGTGA / ATGTCGCTCTCTGCCAAGAT / 60.4 / (GCT)5 / Senthilvel et al., 2008 / Yes* / P
ICMP3069′ / TAGGAGGGGACTGCTCCTTT / AGGAAGAGGATGGTGGTGTG / 62.4 / (ATCC)4 / Senthilvel et al., 2008 / Yes* / P
ICMP3070′ / GGTGTGTTGTGCGGGACT / GTGTGTGTGTGTGTGCCAAG / 62.3 / (CCGTT)3(AC)5(CA)19 / Senthilvel et al., 2008 / No / -
ICMP3072′ / CGCAGCTCTACTTCCAGACC / CAGTAACCAAGAGCACCTCGAT / 63.6 / (CGC)5 / Senthilvel et al., 2008 / Yes / M
ICMP3073′ / GCACGAGGGCCAGATTCTA / TACACGGTGATGACACGACA / 61.4 / (CTG)9 / Senthilvel et al., 2008 / Yes / M
ICMP3074′ / AGGGATTCCAGGCGGTTC / CTCAAGCCACCAGCACAGTA / 62.3 / - / Senthilvel et al., 2008 / No / -
ICMP3076′ / CACGAGGCAGAAGCACATT / CTCTTCTCGGCGATGAGC / 61.2 / (CGCAT)3 / Senthilvel et al., 2008 / No / -
ICMP3079′ / ATGGTAGAGCGGTGAGGTTG / GCAAGGCAATGTAGGTGGAT / 61.4 / (CTTTT)3 / Senthilvel et al., 2008 / Yes / M
ICMP3080′ / CAAACAGCATCAAGCAGGAG / GCGTAGACGGCGTAGATGAT / 61.4 / (AGC)8 / Senthilvel et al., 2008 / Yes / P
ICMP3081′ / ACGCCGTTTTCGTGTAGTCT / TCCACAAGGTGACCTCACTG / 61.4 / (CTG)5 / Senthilvel et al., 2008 / Yes* / P
ICMP3085′ / CTGAAGCTGAAGAGGCCTTG / GGCGGAGATCAGAGTTCG / 62.3 / (TCA)5(TCCG)3 / Senthilvel et al., 2008 / No / -
ICMP3088′ / TCAGGTGGAGATCGATGTTG / TTACGGGAGGATGAGGATG / 60.3 / (TCC)8 / Senthilvel et al., 2008 / Yes / M
ICMP3090′ / AGGTGGCTGTGCGGAGTTAC / GGATGGAGGGGGTCAGTATT / 63.5 / (CACAA)3 / Senthilvel et al., 2008 / No / -
ICMP3094′ / GACCTCGACCTCATCTCCAA / CGACAGCGAACTGGGATTAC / 62.4 / (AAC)5(GAC)4 / Senthilvel et al., 2008 / Yes / P
ICMP3095′ / GGGAGGCCACGATTTAAAGA / ACAATGTGCACGCAAGGA / 59.0 / (TAGAT)3 / Senthilvel et al., 2008 / Yes / M
ICMP3096′ / CTGCATTGCAACATCCTCAC / AACCTGCAGTGGAAGCAATC / 60.4 / (CTT)4(CTT)5 / Senthilvel et al., 2008 / Yes* / P
ICMP4006′ / TGAGGACCGAGAAGAAGCAT / CAACACCCAACAGAAACTGAA / 59.6 / (TG)5(TG)7 / Senthilvel et al., 2008 / No / -
ICMP4009′ / CGAGTGAATGTTGCTGAGGA / ACAATAAGACGGGACGGATG / 60.4 / (TG)6 / Senthilvel et al., 2008 / Yes / P
ICMP4010′ / ATCCCCTACAGCATCAGCAC / CGGCGGAGAGATCTTATTCA / 61.4 / (CCGG)4 / Senthilvel et al., 2008 / Yes* / P
PSMP428″ / CCCGGTTGCAGTAGGTCTAG / AATACTCCATAATCGCCACTCC / 62.7 / - / M.D. Gale, personal communication / Yes / P
PSMP708″ / GCAAGTAACCAACAATCCTTGA / ACCCCTAATACCCAAGCAATT / 65.0 / - / M.D. Gale, personal communication / Yes / M
PSMP737″ / ATGCTTTTCCCCCGCTATCA / TCCTAGCAAGCTCGCATAAGA / 48.8 / - / M.D. Gale, personal communication / Yes* / P
PSMP2008″ / GATCATGTTGTCATGAATCACC / ACACTACACCTACATACGCTCC / 60.8 / (GT)37 / Qi et al., 2004 / No / -
PSMP2019″ / TGTGCCACAGCTTGTTCCTC / CAAGCAGCCAGTTCCTCATC / 62.4 / (CA)38 / Qi et al., 2004 / Yes* / P
PSMP2024″ / ACACCTTAGCAAAGGCTGCTAC / ATGCTTAGTTTGCTATAACTTGGG / 61.1 / (CA)6(TC)9(TG)30 / Qi et al., 2004 / Yes* / P
PSMP2074″ / AGGACTGTAGGAGTGTGGACAA / CCAGACCTACCAGTGAATGAGA / 62.7 / (AC)11 / Qi et al., 2004 / No / -
PSMP2090″ / AGCAGCCCAGTAATACCTCAGCTC / AGCCCTAGCGCACAACACAAACTC / 66.3 / (CT)12) / Qi et al., 2004 / Yes / P
PSMP2202″ / CTGCCTGTTGAGAATAAATGAG / GTTCCGAATATAGAGCCCAAG / 59.8 / (GT)8 / Qi et al., 2001 / No / -
PSMP2203″ / GAACTTGATGAGTGCCACTAGC / TTGTGTAGGGAGCAACCTTGAT / 61.8 / (GT)18 imperfect / Qi et al., 2001 / No / -
PSMP2209″ / TTGGACGATTTGGAAGCATAG / GAGGAAAAGAGCCATACAGAGAC / 60.8 / (GT)6(CT)7 / Qi et al., 2001 / Yes / P
PSMP2210″ / CAATGATGACCGTAATCTGGGTG / GGGCAAGATATGTGAAATCAAG / 60.9 / (GT)12 / Qi et al., 2001 / Yes / P
PSMP2212″ / GATTGGATGGCAGTGCTTGG / CAAACCAGCCATCAACAACCAG / 62.6 / (GT)6 / Qi et al., 2001 / No / -
PSMP2214″ / CGCACAGTACGTGTGAGTGAAG / GATTGAGCAGCAAAAACCAGC / 62.6 / (GT)9 / Qi et al., 2001 / No / -
PSMP2215″ / CCACGTCATTAGAGTAATCCGAG / ACTCAAATCCCAATCTTGAATC / 60.0 / (GT)6 / Qi et al., 2001 / No / -
PSMP2219″ / ACTGATGGAATCTGCTGTGGAA / GCCCGAAGAAAAGAGAACATAGAA / 61.0 / (GT)7 / Qi et al., 2001 / No / -
PSMP2222″ / TGGCTTCCAGACTAATCATCAC / TTATTTTAGCGGCGAGATTGAC / 59.9 / (GT)6 / Qi et al., 2001 / Yes* / P
PSMP2227″ / ACACCAAACACCAACCATAAAG / TCGTCAGCAATCACTAATGACC / 59.9 / (GT)7 / Allouis et al., 2001 / No / -
PSMP2229″ / CCACTACCTTCGTCTTCCTCCATTC / GTCCGTTCCGTTAGTTGTTGCC / 65.4 / (GT)5 / Allouis et al., 2001 / No / -
PSMP2237″ / TGGCCTTGGCCTTTCCACGCTT / CAATCAGTCCGTAGTCCACACCCCA / 67.2 / (GT)8 / Allouis et al., 2001 / Yes / P
PSMP2249″ / CAGTCTCTAACAAACAAACACGGC / GACAGCAACCAACTCCAAACTCCA / 63.8 / (GT)7 imperfect / Allouis et al., 2001 / No / -
PSMP2265″ / GAGATCTATTGGAATTACCGAGAC / CCATACTCAAATCTTCCAACCA / 60.1 / - / S. Allouis, personal communication / Yes / M
Xpsms05‴ / TGCAACTGGCTCACGAAGCTTG / TCATGGCTCCTGGCCTTGAGA / 64.5 / - / Bertin et al., 2005 / No / -
Xpsms08‴ / TAACCGCAGACCACTGCAAC / GCAAGATGAAGGACAGGCTC / 62.4 / - / Bertin et al., 2005 / Yes / P
Xpsms16‴ / CTGACGAAGCTGCCTTACAC / AAGAGTTGCAGAAGAGGCTG / 61.4 / - / Bertin et al., 2005 / Yes / P
Xpsms17‴ / CCCTTCATGGTGAGGATGAG / GACAGAGAAGCTTATCCTGC / 61.4 / - / Bertin et al., 2005 / Yes / M
Xpsms18‴ / ‴TGTGCCATCATCATTCTTGG / CGAGATAGCATCTATGGTGC / 59.4 / - / Bertin et al., 2005 / Yes / M
Xpsms21‴ / CATCAGGTAGTCACCCTTGAC / CTGTATTGGTGCCTGGCATC / 62.5 / - / Bertin et al., 2005 / Yes* / P
Xpsms23‴ / AGAGGACACTGTTGTATGGC / GCTCTGGACTTGGTTCACTG / 61.4 / - / Bertin et al., 2005 / No / -
Xpsms27‴ / TCACGCACGTCATCTTCGAC / CCCATCATCTTGGCCTTGAG / 62.4 / - / Bertin et al., 2005 / Yes* / P
Xpsms28‴ / GATGGAGGAGGAACCAAGCC / TTGCACGCTCGTGCAGTGCT / 64.5 / - / Bertin et al., 2005 / No / -
Xpsms29‴ / CCCTGCGTCAGCATCTCCTG / GGTGGAGGACATCCTCAAAG / 64.5 / - / Bertin et al., 2005 / No / -
Xpsms34‴ / CACTCTCAAGAACTGTTGCAG / TTGAGATCAAGAGTAGAAGGC / 59.7 / - / Bertin et al., 2005 / Yes* / P
Xpsms35‴ / TGACACTCATGTTCACCACTC / CCTGTTAAATCCAAGCTCTC / 59.5 / - / Bertin et al., 2005 / Yes* / P
Xpsms39‴ / CCTGAACGATGTCTCAATACC / ATCAATGAGCCAGAGCTTGC / 60.5 / - / Bertin et al., 2005 / Yes* / P
Xpsms41‴ / TGAGGAGCATTTGTACAGGC / CCATCGATGAGCTTCAGTTC / 60.4 / - / Bertin et al., 2005 / Yes* / P
Xpsms44‴ / GCACCTGCAAGTTTGACAAG / ATGGTCAAGATGCCTTCCAC / 60.4 / - / Bertin et al., 2005 / Yes* / P
Xpsms46‴ / AAGTGGTGGTACTCTGCATTC / ACGATGAGCTGCTGCGGAAC / 62.6 / - / Bertin et al., 2005 / Yes* / P
Xpsms51‴ / AGGAGAAGGCCGACAAGAAG / GAGGAGGTGAACTTTGTGAC / 61.4 / - / Bertin et al., 2005 / Yes / M
Xpsms52‴ / CAGCTTTGCAAGATGGTGAC / GCAGAGGTGTACTGGTTAGC / 61.4 / - / Bertin et al., 2005 / No / -
Xpsms54‴ / TCTGAACCATCTCATCTCTGC / GTCGCACACGCTGACTTCAC / 62.6 / - / Bertin et al., 2005 / Yes* / P
Xpsms55‴ / CTACACCTGGAGCTACACTC / CACAGTACTCCCTTTCAGCA / 61.4 / - / Bertin et al., 2005 / Yes* / P
Xpsms56‴ / GCGGCAGTATCTGTACCGTC / TCTAGAGCCAGTATCTTCCC / 62.5 / - / Bertin et al., 2005 / Yes* / P
Xpsms57‴ / TCTGCGGAGGATCTGACAAC / CGCATGGGACTGAAGCAAAC / 62.4 / - / Bertin et al., 2005 / No / -
Xpsms58‴ / GTTTCATGTCTGATCTCGACG / AGACTCTTTCTGCCGTTGCG / 61.5 / - / Bertin et al., 2005 / Yes / P
Xpsms67‴ / CTACTACCATGACAACCACG / GTACACCATCGTCTTCACAG / 60.4 / - / Bertin et al., 2005 / Yes / P
Xpsms69‴ / TAAACCTCGCCATCCGCGAC / GTTAGTTCCTCGTTCCATTCCG / 63.6 / - / Bertin et al., 2005 / No / -
Xpsms78‴ / GCGCGATCTTGAACCACTCG / GCCATCTTCCTTGACCGCATC / 64.5 / - / Bertin et al., 2005 / Yes / P
Xpsms83‴ / ACCAAGCTTCCAAGGATGAC / CACGCTCTTCAGCAGCCTTC / 62.5 / - / Bertin et al., 2005 / No / -
Xpsms88‴ / AATGCACTAGTCCACCGTCC / CCTACACCACACGCTTCCTC / 63.5 / - / Bertin et al., 2005 / No / -
Xpsms92‴ / TGGTGATGCTGCTGCTTTAG / CGACCGAGTACATCTTCTGG / 61.4 / - / Bertin et al., 2005 / Yes* / P
Xpsms93‴ / TCTCACTTGAAATGGTACAGAG / TCTGGATGACTACTACAGGAC / 59.8 / - / Bertin et al., 2005 / Yes / M
Xpsms101‴ / ACACCTTCCGCATCCTGCTC / GTCTCCATTCACGTTGTCGC / 57.5 / - / Bertin et al., 2005 / Yes / P
Xpsms102‴ / CATGATCTCTATGAGCTCCAG / ATACCCATCAGCTCCGTCTG / 51.3 / - / Bertin et al., 2005 / Yes / M
Xpsms103‴ / GGGAAATTGCACAGGACTTC / GGCATGATGGTCACACGCTT / 52.5 / - / Bertin et al., 2005 / Yes* / P
Xpsms104‴ / CGACTCCGTCAGCCTTCTTG / TTGAAATGCAGCTCCCTAGC / 55.0 / - / Bertin et al., 2005 / Yes* / P

*Used in genetic diversity study.

′EST-SSR

″Genomic SSR

‴SSCP-SNP

M-monomorphic, P-polymorphic

1