Additional file 2 Table S1. Immunopositivity for stem cell reprogramming factors in malignant and benign vascular tumors.

Location / Diagnosis / Type / Oct4 / Nanog / Klf4 / Sox2 / Myc
Heart / Angiosarcoma / Malignant / ++ >75% / + 50-75% / + <25% / +++ 50-75% / + <25%
Fibrous tissue / Angiosarcoma / Malignant / +++ 50-75% / ++ <25% / + <25% / + 25-50% / + <25%
Liver / Angiosarcoma / Malignant / ++ 50-75% / ++ <25% / ++ <25% / ++ 50-75% / ++ <25%
Blood vessel / Angiosarcoma / Malignant / +++ 25-50% / + 25-50% / + <25% / ++ <25% / + <25%
Fallopian tube / Angiosarcoma / Malignant / + 50-75% / ++ 25-50% / + <25% / + <25% / 0
Ankle / Angiosarcoma / Malignant / +++ 50-75% / + <25% / 0 / ++ 50-75% / + <25%
Spleen / Angiosarcoma / Malignant / ++ >75% / ++ <25% / 0 / ++ <25% / 0
Lung / Hemangiopericytoma / Malignant / ++ >75% / + 25-50% / 0 / ++ >75% / 0
Skin / Hemangiopericytoma / Malignant / ++ 50-75% / + 25-50% / 0 / + 50-75% / 0
Heart / Hemangioendothelioma / Borderline / +++ 50-75% / ++ <25% / + <25% / ++ <25% / ++ 25-50%
Skin / Hemangioendothelioma / Borderline / ++ 50-75% / ++ 25-50% / 0 / + <25% / + <25%
Skin / Hemangioendothelioma / Borderline / ++ 50-75% / +++ <25% / + <25% / + 50-75% / + <25%
Ligament / Hemangioendothelioma / Borderline / +++ >75% / +++ <25% / + <25% / ++ <25% / 0
Blood vessel / Hemangioendothelioma / Borderline / +++ >75% / +++ 25-50% / ++ <25% / ++ 50-75% / 0
Blood vessel / Hemangioendothelioma / Borderline / +++ >75% / + <25% / 0 / + 25-50% / 0
Skin / Capillary hemangioma / Benign / +++ 50-75% / +++ 50-75% / ++ <25% / ++ 25-50% / 0
Skin / Capillary hemangioma / Benign / +++ >75% / ++ 50-75% / + <25% / ++ <25% / + <25%
Skin / Capillary hemangioma / Benign / +++ 50-75% / + <25% / + <25% / ++ 25-50% / + <25%
Skin / Capillary hemangioma / Benign / +++ 50-75% / 0 / ++ <25% / + 25-50% / + <25%
Skin / Capillary hemangioma / Benign / ++ 50-75% / +++ 25-50% / ++ <25% / +++ 25-50% / ++ 25-50%
Skin / Capillary hemangioma / Benign / +++ 50-75% / + <25% / + <25% / ++ <25% / + <25%
Liver / Cavernous hemangioma / Benign / +++ 50-75% / +++ 50-75% / ++ <25% / +++ 25-50% / 0
Liver / Cavernous hemangioma / Benign / +++ >75% / + <25% / + <25% / + <75% / + <25%
Liver / Cavernous hemangioma / Benign / + 25-50% / ++ <25% / 0 / + <25% / + <25%
Liver / Cavernous hemangioma / Benign / ++ 50-75% / + <25% / + <25% / + 25-50% / + <25%
Liver / Cavernous hemangioma / Benign / ++ 50-75% / + <25% / + <25% / + 25-50% / ++ <25%
Liver / Cavernous hemangioma / Benign / ++ 50-75% / + <25% / + <25% / + <25% / 0
Liver / Cavernous hemangioma / Benign / ++ 50-75% / ++ <25% / + <25% / + <25% / 0
Liver / Cavernous hemangioma / Benign / ++ 50-75% / +++ <25% / 0 / ++ 25-50% / + <25%
Cerebrum / Cavernous hemangioma / Benign / ++ 50-75% / ++ 25-50% / ++ <25% / ++ 25-50% / + <25%
Liver / Cavernous hemangioma / Benign / ++ 25-50% / +++ 50-75% / ++ <25% / 0 / 0
Liver / Cavernous hemangioma / Benign / ++ 50-75% / ++ 25-50% / ++ <25% / + 25-50% / + <25%
Liver / Cavernous hemangioma / Benign / + 25-50% / + 25-50% / + <25% / + <25% / 0
Liver / Cavernous hemangioma / Benign / ++ 25-50% / + <25% / 0 / + <25% / 0
Liver / Cavernous hemangioma / Benign / ++ 25-50% / ++ <25% / 0 / + <25% / 0
Liver / Cavernous hemangioma / Benign / ++ 50-75% / ++ 25-50% / + <25% / ++ <25% / + <25%
Liver / Cavernous hemangioma / Benign / +++ 50-75% / ++ 25-50% / + <25% / ++ 25-50% / 0
Liver / Cavernous hemangioma / Benign / ++ <25% / ++ 25-50% / + <25% / + 25-50% / 0
Liver / Cavernous hemangioma / Benign / ++ 25-50% / + <25% / 0 / + 25-50% / 0
Liver / Cavernous hemangioma / Benign / ++ 50-75% / +++ <25% / 0 / + <25% / 0
Liver / Cavernous hemangioma / Benign / ++ 50-75% / ++ <25% / 0 / ++ <25% / + <25%
Liver / Cavernous hemangioma / Benign / ++ <25% / +++ 50-75% / 0 / ++ <25% / 0
Liver / Cavernous hemangioma / Benign / +++ 50-75% / + 25-50% / + <25% / + <25% / 0
Liver / Cavernous hemangioma / Benign / +++ 50-75% / +++ <25% / ++ <25% / + <25% / 0
Liver / Cavernous hemangioma / Benign / + 25-50% / + 25-50% / 0 / + <25% / 0
Liver / Cavernous hemangioma / Benign / +++ 50-75% / ++ 25-50% / 0 / + <25% / 0
Liver / Cavernous hemangioma / Benign / +++ 50-75% / + 25-50% / 0 / + 25-50% / 0
Liver / Cavernous hemangioma / Benign / +++ 50-75% / ++ <25% / 0 / + 25-50% / + <25%
Liver / Cavernous hemangioma / Benign / + 25-50% / ++ <25% / 0 / + <25% / 0
Liver / Cavernous hemangioma / Benign / ++ 25-50% / + 50-75% / 0 / + <25% / + <25%
Liver / Cavernous hemangioma / Benign / ++ 50-75% / ++ <25% / 0 / + <25% / 0
Liver / Cavernous hemangioma / Benign / ++ 50-75% / ++ 25-50% / 0 / ++ 25-50% / 0
Liver / Cavernous hemangioma / Benign / ++ 25-50% / +++ <25% / 0 / + <25% / 0
Liver / Cavernous hemangioma / Benign / +++ 25-50% / ++ <25% / 0 / ++ <25% / + <25%
Liver / Cavernous hemangioma / Benign / +++ 25-50% / +++ 50-75% / + <25% / + <25% / 0
Liver / Cavernous hemangioma / Benign / +++ 25-50% / ++ <25% / + <25% / + <25% / 0
Liver / Cavernous hemangioma / Benign / +++ 50-75% / + <25% / + <25% / + 50-75% / 0
Cerebrum / Cavernous hemangioma / Benign / +++ 50-75% / +++ 25-50% / +++ <25% / +++ 50-75% / +++ <25%
Spleen / Cavernous hemangioma / Benign / ++ 50-75% / +++ 50-75% / 0 / ++ 25-50% / + <25%
Tongue / Cavernous hemangioma / Benign / +++ 50-75% / +++ 25-50% / + <25% / +++ 50-75% / + I
Thyroid / Cavernous hemangioma / Benign / ++ <25% / ++ 25-50% / 0 / + <25% / 0
Skin / Cavernous hemangioma / Benign / +++ 50-75% / ++ 25-50% / + <25% / ++ 25-50% / ++ 25-50%
Vulva / Cavernous hemangioma / Benign / ++ 25-50% / ++ 25-50% / + <25% / ++ <25% / 0
Blood vessel / Cavernous hemangioma / Benign / ++ 50-75% / ++ 50-75% / ++ <25% / ++ <25% / + <25%
Spleen / Cavernous hemangioma / Benign / +++ 25-50% / ++ 50-75% / 0 / ++ <25% / 0
Spleen / Cavernous hemangioma / Benign / +++ 25-50% / +++ 25-50% / + <25% / ++ 25-50% / 0
Tongue / Granulomatous hemangioma / Benign / ++ 50-75% / +++ 50-75% / 0 / ++ 50-75% / + <25%
Skin / Granulomatous hemangioma / Benign / +++ 25-50% / + <25% / ++ 25-50% / ++ 25-50% / ++ 25-50%
Skin / Granulomatous hemangioma / Benign / +++ 50-75% / +++ 50-75% / 0 / + 50-75% / ++ <25%
Blood vessel / Phlebitis with thrombus / Benign / ++ 25-50% / ++ 50-75% / + <25% / ++ 25-50% / 0
Mesentery / Venous hemangioma / Benign / +++ >75% / ++ <25% / + <25% / + <25% / + <25%
Carotid artery / Non-diseased / Normal / 0 / 0 / 0 / 0 / 0
Carotid artery / Non-diseased / Normal / ++ 25-50% / 0 / 0 / 0 / 0
Carotid artery / Non-diseased / Normal / + <25% / 0 / 0 / 0 / 0
Carotid artery / Non-diseased / Normal / + 25-50% / + <25% / 0 / + <25% / 0
Carotid artery / Non-diseased / Normal / ++ 25-50% / ++ <25% / ++ <25% / + <25% / 0
Aorta / Non-diseased / Normal / ++ 25-50% / ++ <25% / + 25-50% / ++ <25% / 0
Aorta / Non-diseased / Normal / + <25% / 0 / 0 / + <25% / 0
Aorta / Non-diseased / Normal / ++ 25-50% / + <25% / + 50-75% / + <25% / 0
Aorta / Non-diseased / Normal / + 25-50% / ++ <25% / + 25-50% / + 25-50% / 0
Aorta / Non-diseased / Normal / ++ <25% / 0 / + <25% / 0 / 0

0 = no expression; + = low expression; ++ = moderate expression; +++ = high expression; % = amount of positivity in tissue.

Additional file 2 Table S2. Immunopositivity for stem cell reprogramming factors in a panel of diverse sarcomas.

Location / Diagnosis / Oct4 / Nanog / Klf4 / Sox2 / Myc
Coccyx / Chordoma / +++ 50-75% / ++ <25% / ++ <25% / ++ 50-75% / ++ 50-75%
Coccyx / Chordoma / +++ 50-75% / ++ <25% / ++ <25% / ++ >75% / + <25%
Sacrum / Chordoma / ++ 25-50% / ++ 50-75% / ++ 25-50% / ++ 25-50% / + <25%
Soft tissue, face / Dermatofibrosarcoma protuberans / +++ 50-75% / ++ 25-50% / +++ <25% / + 25-50% / + <25%
Small bowel / Desmoplastic small round cell tumor / +++ 50-75% / + <25% / + <25% / +++ 50-75% / + 25-50%
Soft tissue, thigh / Epithelioid sarcoma / +++ 50-75% / + <25% / ++ 50-75% / ++ 50-75% / + <25%
Soft tissue, thigh / Fibrosarcoma / +++ >75% / + <25% / + <25% / ++ >75% / 0
Kidney / Leiomyosarcoma / ++ >75% / 0 / + <25% / + 50-75% / 0
Uterus / Leiomyosarcoma / +++ >75% / ++ 25-50% / ++ 50-75% / ++ >75% / ++ 50-75%
Abdominal cavity / Leiomyosarcoma / +++ >75% / + 25-50% / ++ >75% / + 25-50% / + 25-50%
Pelvic cavity / Leiomyosarcoma / ++ >75% / ++ <25% / ++ 50-75% / + 50-75% / 0
Soft tissue, thigh / Leiomyosarcoma / +++ >75% / ++ <25% / + <25% / +++ >75% / + 50-75%
Soft tissue, thigh / Leiomyosarcoma / ++ 50-75% / + 25-50% / + <25% / ++ 50-75% / 0
Spermatic cord / Leiomyosarcoma / +++ 50-75% / +++ 50-75% / + 25-50% / +++ 50-75% / ++ 25-50%
Soft tissue, chest / Leiomyosarcoma / +++ 50-75% / + <25% / ++ 50-75% / + >75% / 0
Retroperioneum / Leiomyosarcoma / ++ 50-75% / + <25% / + <25% / +++ >75% / 0
Retroperioneum / Liposarcoma / +++ 50-75% / +++ 25-50% / + 50-75% / + 50-75% / + <25%
Retroperioneum / Liposarcoma / ++ >75% / + <25% / + <25% / + >75% / 0
Abdominal cavity / Liposarcoma / +++ 50-75% / + <25% / ++ <25% / ++ <25% / + <25%
Mesentery / Liposarcoma / +++ 50-75% / ++ 25-50% / ++ <25% / +++ <25% / ++ <25%
Soft tissue, axilla / Liposarcoma / ++ 25-50% / ++ <25% / 0 / ++ <25% / + <25%
Soft tissue, lower leg / Liposarcoma / ++ 50-75% / ++ 25-50% / + <25% / ++ <25% / ++ 25-50%
Soft tissue, thigh / Lipsarcoma / ++ 50-75% / ++ 25-50% / + <25% / + 50-75% / + 25-50%
Femur / Malignant giant cell tumor / +++ 50-75% / +++ >75% / + 25-50% / +++ 50-75% / + 25-50%
Soft tissue, hip / Mesenchymoma, malignant / +++ >75% / ++ <25% / ++ <25% / ++ 50-75% / ++ <25%
Descending colon / Neurofibrosarcoma / +++ >75% / ++ 25-50% / 0 / +++ 50-75% / 0
Soft tissue, forearm / Neurofibrosarcoma / +++ >75% / + <25% / ++ 25-50% / + 50-75% / + <25%
Soft tissue, back / Osteosarcoma / +++ >75% / +++ <25% / +++ <25% / ++ 50-75% / + <25%
Soft tissue, thigh / Osteosarcoma / +++ >75% / +++ <25% / + 50-75% / + >75% / + 25-50%
Pelvic cavity / Perivascular epithelioid neoplasm / ++ 50-75% / ++ 25-50% / ++ <25% / ++ 50-75% / ++ <25%
Soft tissue, knee / Pleomorphic rhabdomyosarcoma / +++ >75% / ++ 50-75% / + 25-50% / +++ 50-75% / ++ 25-50%
Soft tissue, thigh / Pleomorphic undifferentiated sarcoma / +++ 50-75% / ++ 25-50% / ++ <25% / +++ 50-75% / 0
Soft tissue, vertebra / Pleomorphic undifferentiated sarcoma / +++ >75% / + <25% / + 25-50% / ++ 50-75% / + 25-50%
Soft tissue, knee / Pleomorphic undifferentiated sarcoma / ++ 50-75% / + <25% / ++ <25% / ++ 50-75% / 0
Soft tissue, upper arm / Pleomorphic undifferentiated sarcoma / +++ >75% / ++ <25% / ++ <25% / + >75% / + 25-50%
Soft tissue, neck / Pleomorphic undifferentiated sarcoma / +++ >75% / ++ 50-75% / +++ <25% / ++ >75% / ++ 25-50%
Soft tissue, chest / Pleomorphic undifferentiated sarcoma / ++ 50-75% / ++ 25-50% / + <25% / ++ 50-75% / 0
Soft tissue, neck / Pleomorphic undifferentiated sarcoma / +++ >75% / ++ 25-50% / ++ 50-75% / +++ >75% / + 25-50%
Soft tissue, knee / Pleomorphic undifferentiated sarcoma / +++ >75% / ++ 50-75% / ++ 50-75% / +++ >75% / ++ 25-50%
Soft tissue, knee / Pleomorphic undifferentiated sarcoma / +++ >75% / + <25% / + 25-50% / +++ 50-75% / + <25%
Soft tissue, knee / Pleomorphic undifferentiated sarcoma / ++ 50-75% / + 25-50% / + <25% / ++ 50-75% / 0
Soft tissue and femur, thigh / Pleomorphic undifferentiated sarcoma / +++ >75% / ++ <25% / + <25% / ++ 50-75% / + 25-50%
Soft tissue, thigh / Pleomorphic undifferentiated sarcoma / +++ 50-75% / + >75% / + 50-75% / +++ 50-75% / + 50-75%
Abdominal cavity / Primitive neuroectodermal tumor / +++ 50-75% / +++ <25% / + <25% / +++ >75% / + <25%
Soft tissue, pelvis / Primitive neuroectodermal tumor / +++ >75% / + 50-75% / + <25% / +++ 25-50% / ++ <25%
Pelvic cavity / Primitive neuroectodermal tumor / +++ >75% / + <25% / + 25-50% / +++ >75% / + 25-50%
Kidney / Primitive neuroectodermal tumor / +++ 50-75% / ++ <25% / + 25-50% / ++ 50-75% / 0
Soft tissue, cheek / Rhabdomyosarcoma / +++ 50-75% / ++ <25% / ++ <25% / +++ >75% / + <25%
Retroperioneum / Rhabdomyosarcoma / ++ 50-75% / +++ 25-50% / + <25% / + 50-75% / 0
Soft tissue, arm / Rhabdomyosarcoma / +++ >75% / ++ <25% / + <25% / + 50-75% / 0
Soft tissue, shoulder / Synovial sarcoma / ++ 50-75% / +++ 25-50% / + <25% / ++ >75% / + 25-50%
Abdominal wall / Synovial sarcoma / +++ >75% / ++ 50-75% / + 25-50% / +++ >75% / + 50-75%
Soft tissue, thigh / Synovial sarcoma / ++ >75% / + <25% / + 25-50% / ++ >75% / ++ 25-50%
Soft tissue, thigh / Synovial sarcoma / +++ >75% / + 25-50% / + 25-50% / +++ 50-75% / ++ 25-50%
Soft tissue, thigh / Synovial sarcoma / +++ 50-75% / + 50-75% / + 25-50% / ++ 50-75% / + 25-50%
Soft tissue, chest wall / Synovial sarcoma / +++ >75% / ++ <25% / ++ <25% / ++ >75% / + <25%
Soft tissue, pelvis / Synovial sarcoma / +++ >75% / + <25% / + <25% / ++ >75% / 0
Soft tissue, thigh / Synovial sarcoma / +++ >75% / +++ <25% / + 25-50% / +++ 50-75% / + 50-75%

0 = no expression; + = low expression; ++ = moderate expression; +++ = high expression; % = amount of positivity in tissue.