Supplementary Table 1: TF reporters according to biological functional category. Binding sequences for associated TFs and references for binding sequences are also listed. General biological functions of associated TFs are from the Online Mendelian Inheritance in Man Database and the TRANSFAC database.
Category / Reporter Name / Associated TF symbol1 / Apoptosis and DNA repair / E2F1-r / E2F1
2 / Apoptosis and DNA repair / FOXO3A-r / FOXO3A
3 / Apoptosis and DNA repair / SP1-r / SP1
4 / Canonical pathways / β-CATENIN-r / β-CATENIN
5 / Canonical pathways / CREB-r / CREB
6 / Canonical pathways / ELK1-r / ELK1
7 / Canonical pathways / GLI-r / GLI
8 / Canonical pathways / GR-r / GCCR
9 / Canonical pathways / RAR-r / RAR
10 / Canonical pathways / SMAD3-r / SMAD3
11 / Canonical pathways / VDR-r / VDR
12 / Cell cycle and proliferation / AP1-r / AP1
13 / Cell cycle and proliferation / AP3-r / ETS
14 / Cell cycle and proliferation / C-MYC-r / MYC
15 / Cell cycle and proliferation / ETS1-r / ETS1
16 / Cell cycle and proliferation / PTTG- / PTTG
17 / Cell cycle and proliferation / WT1-r / WT1
18 / Cell cycle and proliferation / YY1-r / YY1
19 / Differentiation and development / AP2-r / AP2
20 / Differentiation and development / AP4-r / AP4
21 / Differentiation and development / FOXA-r / FOXA
22 / Differentiation and development / GATA1-r / GATA1
23 / Differentiation and development / GATA2-r / GATA2
24 / Differentiation and development / GATA3-r / GATA3
25 / Differentiation and development / MNX1-r / MNX1
26 / Differentiation and development / RUNX1-r / RUNX1
27 / Differentiation and development / RUNX2-r / RUNX2
28 / Inflammatory response / NFAT-r / NFAT
29 / Inflammatory response / NFκB-r / NFκB
30 / Inflammatory response / STAT1-r / STAT1
31 / Inflammatory response / STAT3-r / STAT3
32 / Inflammatory response / STAT4-r / STAT4
33 / Pluripotency / KLF4-r / KLF4
34 / Hypoxia response / HIF1-r / HIF1A
35 / Wound response / SRF-r / SRF
Continued on the following page.
Supplementary Table 1 (continued).
1 / E2F1-r / E2F Transcription Factor 1 / Cell cycle arrest, apoptosis
2 / FOXO3A-r / Forkhead Box O3A / Apoptosis, DNA repair
3 / SP1-r / Transcription foctor SP1 / Apoptosis, differentiation
4 / β-CATENIN-r / Catenin, Beta 1 / Wnt response, Cell cycle, differentiation
5 / CREB-r / cAMP Response Element-Binding Protein 1 / cAMP response
6 / ELK1-r / ELK1 / MAPK response, proliferation, apoptosis
7 / GLI-r / Glioma-associated Oncogene Homolog / Sonic hedgehog pathway response, transformation
8 / GR-r / Glucocorticoid receptor / Glucocorticoid pathway response
9 / RAR-r / Retinoic Acid receptor / Retinoic Acid response pathway, differentiation, apoptosis
10 / SMAD3-r / Smad3 / TGF-β pathway response
11 / VDR-r / Vitamin D Receptor / Cholecalciferol response, differentiation, immune modulation
12 / AP1-r / Activator Protein 1 / Cell cycle/Proliferation
13 / AP3-r / ETS family TFs / Differentiation, senescence, transformation
14 / C-MYC-r / MYC / Proliferation, transformation
15 / ETS1-r / ETS1 / Proliferation, differentiation, migration, invasion
16 / PTTG- / Pituitary Tumor Transforming Gene 1 / Proliferation, Transformation
17 / WT1-r / WT1 / Proliferation, differentiation
18 / YY1-r / Transcription Factor YY1 / Proliferation, differentiation
19 / AP2-r / Transcription Factor AP2 / Development, transformation
20 / AP4-r / Transcription Factor AP4 / Proliferation, differentiation
21 / FOXA-r / Forkhead Box A1 / Differentiation, development
22 / GATA1-r / Gata binding protein 1 / Hematopoeitic differentiation
23 / GATA2-r / Gata binding protein 2 / Endothelial and adipocyte differentiation, angiogenesis
24 / GATA3-r / Gata binding Protein 3 / Adipocyte differentiation, T-cell differentiation
25 / MNX1-r / Motor-Neuron and Pancreas Homeobox 1 / Differentiation
26 / RUNX1-r / Runt-Related Transcription Factor 1 / Hematopoietic differentiation
27 / RUNX2-r / Runt-related Transcription Factor 2 / Osteogenesis, transformation
28 / NFAT-r / Nuclear Factor of Activated T Cells / Inflammatory response, differentiation
29 / NFκB-r / Nuclear Factor kappa B / Inflammation, transformation, metastasis
30 / STAT1-r / Signal Transducer and Activator of Transcription 1 / Interferon response
31 / STAT3-r / Signal Transducer and Activator of Transcription 3 / Acute phase response
32 / STAT4-r / Signal Transducer and Activator of Transcription 4 / IL-12 response
33 / KLF4-r / Kruppel-like Factor 4 / Pluripotency, differentiation
34 / HIF1-r / Hypoxia Inducible Factor 1A / Hypoxia response, angiogenesis
35 / SRF-r / Serum Response Factor / Serum response, proliferation, differentiation
Continued on the following page.
Supplementary Table 1 (continued).
Reporter Name / Reporter Binding Sequence / Reference for reporter sequence1 / E2F1-r / TTTGGCGCGTAATTTGGCGCGTAATTTGGCGCGTAATTTGGCGCGTAATTTGGCGCGTAA / Panomics
2 / FOXO3A-r / GCTAGCTAAATAAATAAGTAAATAAATATAAATAAATAAGTAAATAAATATAAATAAATAAGTAAATAAATAACCGGT / TRANSFAC ID R09924
3 / SP1-r / GTATTTCCCAGAAAAGGAACGTATTTCCCAGAAAAGGAACGTATTTCCCAGAAAAGGAAC / Panomics
4 / β-CATENIN-r / AAGATCAAAGGGGGTAAGAKCAAAGGGGGTAAAATCAAAGGGGGCCCCCTTTGATCTTACCCCCTTTGATCTTACCCCCTTTGATCTTA / TOPFlash sequence (Millipore)
5 / CREB-r / GCACCAGACAGTGACGTCATGGCCGTCATGACGTCACCCCATTGACGTCAATGGGAGAAC / Panomics
6 / ELK1-r / TTTGCAAAATGCAGGAATTGTTTTCACAGTTTTGCAAAATGCAGGAATTGTTTTCACAGT / Panomics
7 / GLI-r / GACCACCCACGGACCACCCACGGACCACCCACGGACCACCCACGGACCACCCACG / Kim et al 2002; PMID 12042312
8 / GR-r / GGTACATTTTGTTCTAGAACAAAATGTACCGGTACATTTTGTTCTGGTACATTTTGTTCT / Romanov et al 2008; PMID 18297081
9 / RAR-r / AGGTCACCAGGAGGTCACCAGGAGGTCACCAGGAGGTCACCAGGAGGTCA / Panomics
10 / SMAD3-r / TCGAGAGCCAGACAAAAAGCCAGACATTTGCCAGACAC / Panomics
11 / VDR-r / TTCAGGTCAAGGAGGTCAAGGAGGTCAAGGAGGTCA / Panomics
12 / AP1-r / TGACTAATGACTAATGACTAATGACTAATGACTAATGACTAATGACTAATGACTAA / Panomics
13 / AP3-r / CTAGTGGGACTTTCCACAGATCCTAGTGGGACTTTCCACAGATC / Panomics
14 / C-MYC-r / GCTAGCCACGTGCACGTGCACGTGCACGTGCACGTGACCGGT / TRANSFAC ID R02207
15 / ETS1-r / GCTAGCGCGGAAGCGCGGAAGCGCGGAAGCGCGGAAGCGCGGAAGCACCGGT / TRANSFAC ID R02153
16 / PTTG- / GCTAGCTTATCTAACTCGCTGTAGTAATTCTTATCTAACTCGCTGTAGTAATTCTTATCTAACTCGCTGTAGTAATTCACCGGT / Pei 2001; PMID 11115508
17 / WT1-r / CGCCCCCGC / TRANSFAC ID R02262
18 / YY1-r / CGCTCCCCGGCCATCTTGGCGGGTGGTCGCTCCCCGGCCATCTTGGCGGCTGGT / Panomics
19 / AP2-r / CCCCAGGC / Williams et al 1988; PMID 3063603
20 / AP4-r / CAGCTG / Hu et al 1990; PMID 2123466
21 / FOXA-r / GAGTGTTTACTTGAGTGTTTACTTGAGTGTTTACTTGAGTGTTTACTT / Romanov et al 2008; PMID 18297081
22 / GATA1-r / CACTTGATAACAGAAAGTGATAATGATAACAGAAAGTGATAACTCT / Panomics, Weiss et al (in preparation)
Continued on the following page.
Supplementary Table 1 (continued).
Reporter Name / Reporter Binding Sequence / Reference for reporter sequence23 / GATA2-r / GTGAGCAGATGGCGGGGGCTGGCAGGTGAGCAGATGGCGGGGGCTGGCAGGTGAGCAGATGGCGGGGGCTGGCAG / Panomics
24 / GATA3-r / GTTATTTATCTCTTAGTTGTAGTTATTTATCTCTTAGTTGTAGTTATTTATCTCTTAGTTGTA / Panomics
25 / MNX1-r / TTACTAATTAGTGACGTTACTAATTAGTGACGTTACTAATTAGTGACG / TRANSFAC ID M01349
26 / RUNX1-r / TGTGGTTGTGGTTGTGGTTGTGGTTGTGGTTGTGGTTGTGGTTGTGGT / TRANSFAC ID M00271
27 / RUNX2-r / ACCACAACCACAACCACAACCACAACCACAACCACA / TRANSFAC ID R24058
28 / NFAT-r / GGAGGAAAAACTGTTTCATACAGAAGGCGTGGAGGAAAAACTGTTTCATACAGAAGGCGTGGAGGAAAAACTGTTTCATACAGAAGGCGT / Panomics
29 / NFκB-r / GGGAATTTCCGGGAATTTCCGGGAATTTCCGGGAATTTCCGGGAATTTCCGGGAATTTCC / Panomics
30 / STAT1-r / CATGTTATGCATATTCCTGTAAGTGCATGTTATGCATATTCCTGTAAGTGCATGTTATGCATATTCCTGTAAGTG / Panomics
31 / STAT3-r / TGCTTCCCGAATTCCCGAATTCCCGAATTCCCGAATTCCCGAATTCCCGAACGT / Panomics
32 / STAT4-r / CTAGAGCCTGATTTCCCCGAAATGATGAGCTAGCTAGAGCCTGATTTCCCCGAAATGATGAGCTAG / Panomics
33 / KLF4-r / GCTAGCAAAGAAAAAAAAGGGAAGGAGGGAAAGGGAAGGAGGGAAAG GAAGGAAGGAAGGGACCGGT / TRANSFAC
34 / HIF1-r / GTGACTACGTGCTGCCTAGGTGACTACGTGCTGCCTAGGTGACTACGTGCTGCCTAGGTGACTACGTGCTGCCTAG / Panomics
35 / SRF-r / GGATGTCCATATTAGGACATCTGGATGTCCATATTAGGACATCTGGATGTCCATATTAGGACATCT / Panomics