DR. AMJAD KHALIL
ASSOCIATE PROFESSOR OF BIOTECHNOLOGY
DEPARTMENT OF PHYSICS
Building 6-Room 134
Ext: 7152
Course Title: Principles of Biotechnology (Under Special topics in Physics
(PHY493)
Course Description:
This course discusses the fundamental aspects of biotechnology and its importance to mankind in a concise and lucid explanation of this newly founded science. The course emphasizes how cell structure and function is a vital starting point for knowledge of genetic engineering and gene technology. This course explores the fundamental issues of biotechnology and will have an answer to the main fundamental questions, such as what is biotechnology and how did it come about? How is biotechnology done and how is it being used today? What are the consequences of biotechnology applications to the lives of humans and other animals, plants and to the environment? Finally, what are some of issuesthat biotechnology raises about the role of science and technology in society?Lectures will underline the principles and application of Recombinant DNA technology in industrial, agricultural, pharmaceutical, and biomedical fields.Bioinformatics and analysis of biological data using computer software will be introduced.
Course Objective:
- To describe the fundamental aspects of biotechnology
- To describe the importance of biotechnology to mankind
- To underline the principles of Recombinant DNA technology
- To describe the applications of microorganisms in industry, environment, agriculture and biomedical fields.
- To underline the application of Recombinant DNA technology in plant and animal biotechnology.
- Give examples of biotechnology products and biotechnology techniques.
- Discuss the importance of transgenic Plants, Bacteria and Animals.
- To define what is bioinformatics and it importance for analysis of data related to biotechnological applications.
COURSE CONTENT
Week / Topics / Chapter in Text (handout)1 / Modern Biotechnology
What is Biotechnology
Ancient Biotechnology
Classical Biotechnology
The Foundation of Modern Biotechnology / 1
2 / From DNA to proteins
Central Dogma of Molecular Biology
DNA Replication
Transcription & Translation / 2
3-5 / Basic Principles of DNA Technology
Restriction Enzymes, Cloning Vectors and Host Cells
Expression of Cloned Genes
Problems Associated with Gene Expression
Cloning strategies
Selection of cloned Genes
Expression systems
Applications of DNA Technology
Human Therapy, Industry & Agriculture
Environmental Protection / 3
6-8 / Microbial Biotechnology
Fermentation, Fermentation Systems,
Fomenter Design
Upstream and Downstream Processing
I-Production of Whole cell Biomas (starter culture, single-cell protein, bacterial vaccines)
II-Micoorganisms and agriculture
Ice – Minus Bacteria, Microbial Pesticides
III Products from microorganisms
a-Low M.Wt.compounds
1-Metabolic End Products
2-Essential Metabolites
3-Bioconversions
b-High M.Wt. Compounds
1-Microbial Polysaccharides
2-Plastics
3-Proteins
c-Fuels
IV-Process Dependent on General Microbial Metabolism
1-Bioremediation
2-Oil Spills
3-Degradation Pathway
4-Waste Water Treatment
5-Heavy Metals
6-Oil and Mineral Recovery / 5
9-10 / Plant Biotechnology
Plant Genetic Engineering
Applications of Plant GE in:
Corp improvement, Herbicide Resistant, Insect Resistant, Virus Resistant, Plants as Bioreactors, Risk of Genetically Modified Organisms (GMOs)
Possible Effects of Releasing GMOs into the Environment / 6
11 / Animal Biotechnology
Gene Transfer Methods in Animals
1-Microinjection
2-Embryonic stem cell Gene Transfer
3-Retrovirus and Gene Transfer
4-Transgenic Animals & their applications
5-Human Interference in Animal Biotechnology / 7
12-13 / Medical Biotechnology
Gene therapy
Gene Therapy Models
1-Liver Diseases (LDLR&FH)
2-Lung Diseases (Cystic Fibrosis)
- / 10
13-14 / Introduction to Bioinformatics in Biotechnology- / Hand-out
Prerequisite: None
Faculty
Dr.Amjad Khalil
10. Textbook:
Title: Biotechnology, An introduction
Author(s): Susan, R. Barnum
Publisher: Thomson Learning/Thomson Brooks/Cole USA
Year: 2005
Edition: 2nd
Book Website:
References:
The following references are extra useful resources of learning for the students.
1- Molecular Biotechnology: Principles and Practices (Hardcover)
by Channarayappa (Author)
Publisher: CRC; 1 edition (2007)
ISBN-10: 1420051571
ISBN-13: 978-1420051575
2- Biotechnology from AtoZ, 2nd ed.
ISBN-13 978-0-19-963693-8
Publisher: W. Bains, IRL Press at OxfordUniversity Press, 1998
3- - Molecular Biotechnology, Primorse, 1991
4- Molecular Biotechnology, Glick & Pasteinak, 1994
5- Recombinant DNA, Watson, Witknowski & Zoller, 1993
6. Environmental Biotechnology, Alan Scragg, 1999
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