HA-UPRT cloned into pBluescript SK- at XbaI – SalI sites
ATGTACCCCTACGATGTGCCCGATTACGCCACTAGTGCTAGCAGATCTGCGGCCAACATGGCGCAGGTCCCAGCGAGCGGAAAGCTCCTTGTCGATCCCCGATATTCGACAAACGACCAGGAAGAAAGCATTCTCCAGGACATCATCACGAGGTTTCCCAATGTGGTGCTCATGAAGCAGACGGCTCAGCTTCGAGCGATGATGACCATCATTCGTGATAAAGAAACACCGAAGGAAGAATTCGTCTTCTACGCCGACCGCCTGATTCGCCTCCTCATCGAAGAAGCTTTGAACGAACTGCCGTTCGAAAAGAAGGAAGTGACAACCCCTCTGGATGTGTCATACCATGGAGTTTCCTTCTATTCCAAGATCTGTGGCGTCTCGATTGTGAGAGCTGGCGAGTCGATGGAAAGCGGCTTGCGGGCAGTTTGCCGCGGCTGCCGCATCGGGAAAATCCTCATCCAGAGAGACGAAACAACTGCGGAGCCTAAGCTGATCTACGAGAAGCTGCCTGCCGACATTAGAGATCGCTGGGTGATGCTGCTAGATCCGATGTGCGCGACGGCGGGCAGTGTGTGCAAAGCGATCGAGGTCCTCCTGAGGCTCGGCGTGAAGGAAGAGAGAATCATTTTCGTCAACATTTTGGCTGCTCCCCAAGGCATTGAACGTGTTTTCAAGGAATACCCGAAAGTCCGCATGGTCACTGCTGCTGTTGACATCTGCCTGAACTCGAGGTACTACATCGTCCCCGGCATTGGTGATTTCGGTGACCGGTACTT
TGGAACCATGTAG
The first 57 nucleotides encode HA epitope followed by “spacer”
HA peptide = YPYDVPDYA
The ATG in bold is start of UPRT sequence (from ME49 strain of T. gondii)