Curriculum Vitae Page 1MiroslawP.Ruka
Curriculum Vitae
MIROSLAWP.RUKA
D.V.M., Ph.D.
Total Number of Words Published (TWP): 8,891
Address for correspondence:
Dr.MiroslawP. Ruka
Professor Veterinary Surgery
The Cambridge Center for Medical & Veterinary Sciences Inc.
55 Yarmouth Street, Suite 204, Guelph, Ontario,N1H 7R4
Canada
Telephone / Fax : (519) 763-9192 E-mail:
Curriculum Vitae Page 1MiroslawP.Ruka
PERSONAL:
Place of Birth:Warsaw, Poland
Citizenship:Canadian
Country of residence:Canada (since July 1989 – present)
Address of permanent204-55 Yarmouth Street
residence:Guelph, OntarioN1H 7R4, Canada
Tel.: / Fax: (519) 763-9192
Present address ofThe Cambridge Center for Medical & Veterinary Sciences Inc.
employment:55 Yarmouth Street, Suite 204, Guelph, OntarioN1H 7R4, Canada
Telephone: / Fax: (519) 763-9192
E-mail:
EDUCATION:
1964-68High School; J.Slowacki - Lyceum No. 7, Warsaw, Poland
1968-74WarsawVeterinarySchool, Faculty of Veterinary Medicine, Academy of Agriculture, Warsaw, Poland
1974Degree: Doctor of Veterinary Medicine (D.V.M.) obtained from the WarsawVeterinarySchool, Faculty of Veterinary Medicine, University of Agriculture and Ministry of Agriculture in Warsaw, Poland, to practice a veterinary medicine as a General Veterinary Practitioner in its entirety.
1975-80Degree: Doctor of Natural Science (Ph.D.) in Experimental Medicine obtained from the PolishAcademy of Sciences, Faculty of Medicine, Medical Research Centre, Warsaw, Poland
Title of Ph.D. Thesis: "Impact of Hyperosmolar and Hyperoncotic Solutions on Water Distribution in Organs to be Transplanted".
VETERINARY LICENSES:
1985Dutch license: to practice as a Doctor of Veterinary Medicine (D.V.M.), signed by Her Majesty Beatrix, Queen of The Netherlands, etc., etc., obtained with admission to the Practice of Veterinary Medicine in its entirety from the Faculty of Veterinary Medicine, University of Utrecht, and Ministry of Agriculture and Fisheries in The Hague, Holland.
1995Canadian license: obtained with admission to the Veterinary Medicine Practice as a Veterinary Small Animal Organ Transplant Surgeon(exclusively = restricted), issued by the Registration Committee of the College of Veterinarians of Ontario (CVO), GCL-license no. 4796.
MAJOR FIELDS OF SPECIALIZATION:
Clinical and comparative medical and veterinary small animal organ/tissue transplantation
SPECIAL FIELDS OF SPECIALIZATION FOR Ph.D.:
Small animal soft tissue surgery, organ transplantation, organ preservation, organ procurement, and transplantation related immunology
LANGUAGES:English; Polish
PRESENT POSITION(S)held at The Cambridge Center For Medical & Veterinary Sciences Inc.:
Faculty Member: - Professor Veterinary Surgery
Senior Executive Positions: - 1stDirector, Chairperson, President and Secretary
- Voting Member of the Board of Directors,
- Founder & Owner
PRESENT POSITION(S)heldat the Clinical & Comparative Medical & Veterinary Sciences Journal:
- Publisher
- Founder & Owner
The History of EMPLOYMENT:
*DEPARTMENT OF EXPERIMENTAL SURGERY & TRANSPLANTATION, Medical Research Centre, Polish Academy of Sciences, Faculty of Medicine, Warsaw, Poland
Dr.W.A.Rowinski - Professor of Surgery; Dr. W.L. Olszewski - Professor of Surgery& Chairperson;
-From May 1975 to December 1984; Scientific Positions (in transplant/experimental surgery):
- 1975-1980 - Assistant; 1980-1984 -Adjunct=Associate Professor of Experimental (Transplant) Surgery
*DEPARTMENT OF EXPERIMENTAL SURGERY, Faculty of Medicine, University of Groningen, Groningen, Holland; Dr. Ch.R.M. Wildevuur - Professor of Surgery& Chairperson;
*CENTRAL ANIMAL LABORATORY, Faculty of Medicine, University of Groningen, Groningen, Holland; Dr.H. Dikken - Director of the C.A.L;
-From January 1984 to January 31, 1985; Scientific Position: Post-Doctoral Fellowship
*THE VETERINARY SMALL ANIMAL CLINIC; M.A. van Messel and J.D.Th. Straatman, Groningen, Holland; From August 1985 to August 31, 1986; Position: Veterinary Small Animal Surgeon (Surgery)
*DEPARTMENT OF ORGAN TRANSPLANTATION, Faculty of Medicine, Kuwait University, Kuwait;
Dr. G.M. Abouna - Professor of Surgery and Chairperson;
-From August, 1986 to August 31, 1989;
Scientific Position: Assistant Professor of Experimental (Transplant) Surgery.
*DEPARTMENT OF CLINICAL STUDIES, Faculty of Veterinary Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada; Dr. D.G. Butler - Professor of Medicine and Chairperson;
-From June 1990 to June 18, 1991;
Scientific position: Post-Doctoral Fellowship (in Transplant Surgery)
-From June 19, 1991 to June 1997.
Scientific position: Associate Research Scientist in Experimental (Transplant) Surgery
*THE Centre of Clinical & Comparative Medicine Inc. (CCCM), Guelph, ON, Canada;
Mr.RonaldW.P.Struthers - C.E.O. & President
Positions held-from June 1997 to November 6, 1998:
06-1997 to 1998Professor of Clinical Small Animal Transplant Surgery & Experimental Surgery
06-1997 to 1998Director, Clinical Small Animal Organ Transplant Programs & Services
06-1997 to 1998Director, Xenotransplant Programs & Services
Nov. 1998Vice-President for Research and Senior Chief Scientific Officer
*The cambridge Center for Medical & Veterinary Sciences INC. (CMVS);
Guelph, ON, Canada ( Dr. M.P. Ruka, Founder & Owner;
Position(s) held from November 26, 1998 to present:
Professor Veterinary Surgery;1stDirector;Chairperson of the Board of Directors;Voting Member of the Board of Directors;President and Secretary; Founder & Owner
*The GuelphAnimalHospital (GAH, Guelph, Ontario, Canada)
Dr.RobertButler - Director and Owner
Position(s) held:Veterinary Small Animals Soft Tissue Surgeon(Surgery)
From January 15, 2003 to November 01, 2010
*Clinical & Comparative Medical & Veterinary Sciences Journal, Guelph, Ontario, Canada ( M.P. Ruka, Founder & Owner;
Position(s) held from July 10, 2007 to present:Publisher; Founder & Owner
MAJOR FIELDS OF CLINICAL, APPLIED AND BASIC RESEARCH INTERESTS:
(1.)CLINICAL ORGAN TRANSPLANTATION: kidney and pancreas allograft transplantation into uremic or/and diabetic patients (dogs and cats), particularly:
-selection of the donor and donor-recipient matching system
-donor pre-treatment, "untouched" organ harvesting technique and cold storage for transplantation
-selection of flushing/preservation solution for temporally cold (4°C) storage
-recipient pre-treatment prior to organ transplantation
-treatment of post-reperfusion injury
-recipient post-operative management
-induction of the modern immunosuppressive protocol to prevent acute or chronic rejection of transplanted organ and to achieve its permanent accommodation (transplantation tolerance)
-selection of antibiotic therapy
-short- and long-term evaluation of graft(s) functional survival post-transplantation
(2.)EXPERIMENTAL SOLID ORGANS / TISSUES PRESERVATION TRANSPLANTATION(liver, kidney, pancreas, islet cells, heart, lungs, ileum) in animals, such as dogs, pigs, rabbits, rats, particularly:
-suppression of the harmful effect of warm and cold ischemia on stored organs prepared for transplantation (e.g. dehydration phenomenon, and/or Ca++ channel blockers).
-mechanism of tissue fluid formation and its transport through the capillary wall and across the interstitial space in ischemic tissue(s).
-post-transplantation flushing/ischemic reperfusion injury and tissue inflammatory response in organs exposed to short and long-term warm/cold ischemia (eg. generation of free radicals, IL-1; IL-6 and other cytokines; TNF; adhesion molecules; ß-thromboglobulin), and their effects on acute and/or chronic tissue rejection in auto-, allo- and xeno-graft models of kidney transplantation.
(3.)XENOGENIC TRANSPLANTATION OF SOLID ORGANS TISSUES IN DISCORDANT ANIMALS: The mechanism of hyperacute/acute - vascular/tissue rejection in immediately or cold (4°C)
stored vascularized kidney xenograft transplanted from:
- unmodified pig-donor to modified (any)-recipient by: classic immunosuppression (CsA, brequinar sodium, etc.), plasmapheresis, donor bone marrow, total body irradiation, splenectomy, etc.
- modified pig-donor to modified (any)-recipient: e.g. production of transgenic/chimeric pig/(recipient)
- modified pig-donor to unmodified (any)-recipient, particularly: to overcome the problem of hyper/acute and/or accelerated vascular/tissue rejection and to achieve a permanent accommodation of the xenograft.
(4.)ORGAN RECONSTRUCTION: Functional reconstruction of the bile duct, oesophagus, trachea, urinary bladder, cornea (intracorneal lenses) and continuity of the vascular system using animals such as dogs, rabbits and rats, particularly:
-mechanism of biomaterial interaction (e.g. PEU, PLL, PU, carbon fiber) with the tissues/fluids of the body, and effects of the tissue reactivity on changes in permeability, electrical, optical and mechanical properties of the novel artificial materials used for reconstruction.
(5.)ETHICAL ISSUES OF USING ANIMALS for:
a.) Medical clinical, applied, and/or basic research;
b.) Teaching in medical, veterinary and other biologically oriented schools/universities;
c.) In a clinical veterinary setting andits application for human and animals benefit;
d.)Education of the new generation of physician/veterinarian scientists/researchers and/or biologists in medical and/or veterinary and other related disciplines.
PROFESSIONAL EXPERIENCE:
(1.)GENERAL CLINICAL VETERINARY PRACTICE:In Poland (Warsaw), in Holland,at M.A. van Messel and J.D.Th. Straatman’s Small Animal Hospital in Groningen, and in Canada, Guelph, Ontario (at Dr. Robert Butler’s Guelph Animal Hospital), in the veterinary clinical small animal practice (mainly dogs and cats), including:
a)Surgery, such as ovariectomy, ovariohysterectomy, testectomy, prostatectomy, mastectomy, penile amputation, enterectomy, subcutaneous or pinna hematoma, gastric torsion, bone fracture, patellar reposition, hip dysplasia, extirpation of anal glands, lumpectomies, etc.;
b)Radiology of hips, long bone fractures, abdomen and thorax cavitiesusing digital radiography ;
c)Dental surgeries and dental preventive medicine;
d)Anesthesia: (1) sedation with e.g. meperidine or ketamine, acepromazine, atropine; (2) induction with e.g. thiopental, (3) surgical anesthesia with isoflurane/halothane and oxygen and (4) pre-/post-operative animal care;
e)Treatment of diseases such as skin allergies due to external/internal parasites or food, otitis external, endometritis, Vit. D deficiency, prevention of heat and pregnancy in bitches, false pregnancy, parvovirus infections, diabetes, kidney and liver diseases, etc.;
f)Vaccination of dogs for hepatitis, adeno type 2, parainfluenza, leptospirosis, parvovirus, rabies and cats with felocell, felocine, felocell RC, felocell CVR, FelV, rabies.
(2.)ORGAN TRANSPLANTATION in CLINICAL and COMPARATIVE MEDICINE:
In Poland (Polish Academy of Sciences, Warsaw),in Kuwait (University of Kuwait, Kuwait City) and in Canada(University of Guelph, OntarioVeterinaryCollege and currently at The Cambridge Center for Medical & Veterinary Sciences Inc., in Guelph, Ontario):
(a)participating in Human Organ Transplant Program(s) as a second assistant in the surgeries to obtain kidneys from living related donors or from cadaver donors, kidney procurement and storage, and during their transplantation into human recipients;
(b)research and surgery (including microsurgery) training in the area of small animals (dog, swine, monkey, rabbit, rat, and mouse) soft tissue surgery (liver, lung, heart, spleen, skin) and vascular surgery involving kidney, liver, pancreas, ileum, lymph node auto-, allo-, xeno-transplantation, using various immunosuppressive protocols to prolong xenograft and/or allograft functional survival (including more than 250 kidney auto/allo-transplantation in dogs);
(c)performing lymphography, angiography, urography, inulin renal function test, and the measurement of changes in total tissue water concentration in various organs.
(d)microsurgery - collecting of the rat pancreas for preservation and transplantation using operating microscope (see Appendix II).
(e)investigating the influence of various aspects of organ procurement on pre- and post-transplant organ viability, involving pancreas (monkey and rats), liver (dog-to-dog) or islet of Langerhans ( monkeys, pigs and rats) allo-transplantation.
(f)evaluating of commonly used organ preservation solutions on abrogation of tissue edema and suppression of tissue autolysis following 96 hrs cold storage of rat/dog/pig pancreas/kidney (including solution's pH, osmolarity, oncotic pressure, -COOH group, free radical scavengers, and low/high molecular weight of selected ingredients of cold storage solutions, perfusion pressure, donor fasted vs. non-fasted, etc.);
(g)reassessing the effect of kidney collection, storage and transplantation on organ function post-transplantation using a pig auto-transplantation protocol following UNOS standards;
(h)reassessing pig kidney hyper-acute rejection following transplantation into the laboratory beagle dog with and without natural anti-pig antibody depletion by extracorporeal circulation of the recipient's blood through the donor pig's liver;
(i)successfully creating, in utero, a 50% pig/dog chimera (assessed by DNA flow cytometric analysis based on DNA content) by the injection of dog bone marrow cells into the abdomen of the fetal pigs; (e) development of surgically-induced endometriosis in a mouse model, etc.;
(j)currently establishing, implementing and promoting at The Cambridge Center for Medical & Veterinary Sciences Inc. (CMVS)clinical, teaching, research and diagnostic programs in the field of small animal (dogs and cats) medicine, small animal surgery, and small animal organ and tissue transplantation (in comparative studies with a human being) and planned to be expanded by a construction (in GTA) of 280,000-square foot facility, a 7-level building (that includes 4 underground levels and 3 levels above the ground, to be build in accordance with the human hospital's standards and specifications) named here as a Hospital (teaching- learning Hospital) in a number of stages and sub-stages. For more information, please refer to the company's web page:
(3.)TEACHING:
-Supervising and teaching senior, junior and technical staff the scientific methodology used in biological experiments, surgical and microsurgical techniques on animals, and animal care before and after operation, in agreement with Canadian Council on Animal Care (guide to the Care and Use of Experimental Animal, Vol. 1 & 2) and Animals for Research Act, Ontario, 1983.
-Seminars/lectures for 4th year students and senior/junior technical staff on the subject of organ transplantation and transplantation related immunology - "Elective in Veterinary Clinical Immunology" course (Canada).
-Seminars/lectures for under-graduate students on the subject of surgery (type of incisions, anatomy of abdominal wall, wound healing, tissue trauma/ischemia, surgical infections, shock, trauma, metabolism and the role of endocrine and immune systems in surgery, hemeostasis, etc.) including pre-operative preparation and post-operative management of surgical patients (Canada).
-Preparation of under-graduate and graduate students for their oral presentations or written assignments on subjects related to organ transplantation and/or general surgery (Canada).
(4.)SEMINARS/LECTURERS given for UNDER/GRADUATE and POST-GRADUATE courses:
Human Living Organ Donors - Source of the Perfect Organ: The Risk
Transplantation Tolerance
Transplantation and the Religious Believes
Diagnosis of Rejection vs. Infection and Drug Toxicity
Clinical Organ Transplantation in Companion Animals
Treatment of Allo-Graft Rejection
Diet in Organ Transplant Recipients
Organ Transplantation (Medical Research) and Animal Rights Activists
Ethical Issues of Using Animals for Medical Research
(5.)ADMINISTRATION:
- Organizing work at the operation theater and at the small animal (dog) farm. (at the Department of Experimental Surgery and Transplantation, MedicalResearchCenter, Faculty of Medicine, Polish Academy of Sciences, Warsaw, Poland, 1975-1983)
- Supervising redistribution of incoming and outgoing pharmaceuticals for the small animals such as dogs, pigs, rabbits, monkeys and rats (at the Department of Experimental Surgery and Transplantation, Medical Research Center, Faculty of Medicine, Polish Academy of Sciences, Warsaw, Poland, 1975-1983).
- Appointed to Associate Professor Position at t the Department of Experimental Surgery and Transplantation, MedicalResearchCenter, Faculty of Medicine, PolishAcademy of Sciences, Warsaw, Poland, 1980-1984.
- Acting Chairman in the absence of the departmental chairman (on a few occasions in Poland and Kuwait).
- Organizing the departmental research seminars (Poland, Kuwait).
- Organizing Microsurgical Laboratory at the OntarioVeterinaryCollege (at the Department of Clinical Studies, OntarioVeterinaryCollege, OVC, University of Guelph, Guelph, Ontario,Canada, 1990-1997).
- Evaluation of surgical procedures on behalf of the Animal Care Committee, University of Guelph, Ontario (Canada, 1995).
- Writing Manual for the CCCM’s Faculty & Staff Position Interview(s), Guelph, ON (Canada, 1997).
- Establishing and writing the Guidelines for the CCCM’s Ethics Committee for Clinical & Comparative Medicine (internal use)Guelph, Ontario (Canada, 1997-1998).
- Establishing The Cambridge Center for Medical & Veterinary Sciences– (Dr. M.P. Ruka, Founder & Owner) for the purpose of raising US$16.5 million to build according to the human hospital’s specifications in Greater Toronto Area (GTA) a teaching-learning 300,000-square-feet facility - Small Animal Hospital (name still to be decided in accordance with the College of Veterinarians of Ontario regulations), where the clinical, teaching, research with diagnostic labs and services programs toassess the strategies to be undertaken by professionals in their fields of expertise (veterinary, human medicine, biology, etc.) in organ/tissue/stem cells transplantation/regeneration, oncology, and genome for the public health - the benefit of humans and animals - will be carried out – (Canada, From November 1998 – to present).
- Dr. Ruka is Chairperson of the CMVS Board of Directors, First Director, and thePresident & Secretary at The Cambridge Center for Medical &Veterinary Sciences Inc. (CMVS) (Canada, 1998-to present).
- Holding Faculty position of Professor Veterinary Surgery at The Cambridge Center for Medical &Veterinary Sciences Inc. (CMVS) (Canada, 2007-to present).
- Writing The CMVS Manual of Organ/Tissue Transplantation for Humans and Animals Benefit – Development, Programs & Regulations (Canada, 1999 - to present).
- Writing, preparing and completing the Handbook for the CMVS' Programs Investor(Business Plan) and its version for the Canadian Government,as well (with 10 Chapters, Canada 2000-2001).
- Designing, writing, preparing and up-dating The Cambridge Center for Medical &Veterinary Sciences Inc. (CMVS) web page - 2004 – to present).
- Establishing on-line an-open Clinical & Comparative Medical & Veterinary Sciences Journal – (Dr. M.P. Ruka, Founder & Owner, Publisher) providing an instant publishingsystem (in less than 24 hours) for the professional colleagues around the World, the authors' of manuscripts presenting their various clinical, teaching, applied research ideas, new concepts, techniques, point of views and/or novel methods of treatment which are published without any delay(s), and for the purpose of securing and preserving the author's intellectual rights, as well. Journal’s web page: 2007-to present).
RESEARCH PROJECTS FUNDED:
1986-1987The effect of isoosmolar, hyperosmolar and hyperoncotic solutions on rat pancreas stored at 4°C for up to 96 hrs (Principal investigator, grant from the University of Kuwait in the amount of C$4,400.00).
1990-1992The effect of in vivo flushing and simple cold (4°C) preservation in UW Belzer solution on the wet weight and morphology of the rat/dog pancreas and pig/dog kidney. (Principal investigator, grant from the Department of Clinical Studies, OVC, University of Guelph, Guelph, Ontario, Canada in the amount of C$5,000.00).
1990-1992The effect of flush-preservation solution with or without free radical inhibitors on the change in the wet weight and morphology of rat pancreas exposed to cold ischemia (4°C) for a period of 96 hours (Principal investigator, grant from the Department of Clinical Studies, OVC, University of Guelph, Guelph, Ontario, Canada in the amount of C$5,000.00).
1991-1993Use of (31P)-MRS and MRI (1H) to assess pre- and post-transplantation pig and dog kidney viability (Principal investigator, cooperation with Dr.E.Janzen, MRI Facility).