electronic supplementary material
sediments, sec 1 • sediment quality and impact assessment • research article
Characterization and optimization of the preparation procedure for solution P-31 NMR analysis of organic phosphorus in sediments
Di Xu • Shiming Ding • Bin Li • Fei Jia • Xiang He • Chaosheng Zhang
Received: 4 January 2012 / Accepted: 16 March 2012
© Springer-Verlag 2012
Responsible editor: Leo Condron
D. Xu • S. M. Ding (*) • X. He
State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, P. R. China
e-mail:
B. Li
College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, P. R. China
F. Jia
College of Environmental Science and Engineering, Hohai University, Nanjing, China
C. S. Zhang
GIS Centre, Ryan Institute and School of Geography and Archaeology, National University of Ireland, Galway, Ireland
(*) Corresponding author:
Shiming Ding
Phone: 86-25-86882207
Fax: 86-25-86882207
e-mail:
Table S1 Results from three replicate analyses of the sediment extracts by 31P NMR. Values include errors from the preparation procedure prior to 31P NMR analysis.£
Lake Taihu
Concentration
(mg kg-1) / Mean# / 251.4 ± 10.1 / 62.4 ± 3.8 / 8.7 ± 1.6 / 22.1 ± 2.3 / 2.3 ± 0.5 / n.d.
CV‡ / 4 / 6 / 18 / 10 / 21 / -
Proportion
(%) / Mean / 72.5 ± 1.5 / 18.0 ± 0.7 / 2.5 ± 0.4 / 6.4 ± 0.4 / 0.7 ± 0.2 / n.d.
CV / 2 / 4 / 16 / 6 / 29 / -
Lake Qinghai
Concentration
(mg kg-1) / Mean / 22.8 ± 4.7 / 30.9 ± 2.3 / 6.4 ± 1.2 / n.d. / n.d. / 1.7 ± 0.6
CV / 2 / 7 / 19 / - / - / 35
Proportion
(%) / Mean / 36.8 ± 1.8 / 50.0 ± 5.2 / 10.4 ± 1.5 / n.d. / n.d. / 2.8 ± 0.7
CV / 5 / 10 / 14 / - / - / 26
£ Triple extraction of the lyophilized sediments from each lake was performed with 0.25 M NaOH-50 mM EDTA using a solid:solution ratio of 1:8 (w/v) for 16 h at 20 °C. The extracts were concentrated to ~10 times of their original concentrations in a rotary vacuum evaporator at 28 °C.
§ orthoP, orthophosphate; monoP, orthophosphate monoesters; lipidP, phospholipids; pyroP, pyrophosphate, phonateP, phosphonates.
# Values are means ± standard deviation of three replicate extracts.
‡ Calculated as 100 (standard deviation/mean).
£ n.d., not detected.
Fig. S1 Duplicate analyses of the sediment extracts from Lakes Taihu and Qinghai by 31P NMR, respectively. Triple extractions of the lyophilized sediments from each lake were performed with 0.25 M NaOH-50 mM EDTA using a solid:solution ratio of 1:8 (w/v) for 16 h at 20°C. The extracts were concentrated to ~10 times of their original concentrations in a rotary vacuum evaporator at 28°C
Fig. S2 31P NMR spectra of the extracts from sediments of Lake Qinghai with different preparation treatments. The sediments were extracted with 0.25 M NaOH-50 mM EDTA using a solid:solution ratio of 1:8 (w/v) for 16 h at 20°C
Fig. S3 31P NMR spectra of the extracts from the sediment of Lake Qinghai. The lyophilized sediments were extracted using different extractants using a solid:solution ratio of 1:8 (w/v) for 16 h at 20°C