SNA Symposium 2017
5th July 2017
Centre for Life Sciences
National University of Singapore
Singapore 117456
For Official Use:
Registration / Abstract ID:
- Please E-mail the completed registration form and abstract to:
- In addition, please mail a hardcopy of the registration form AND abstract to:
Singapore Neuroscience Association, c/o: Department of Anatomy, Yong Loo Lin School of Medicine
MD10, 4 Medical DriveSingapore 117594
- For enquiries, please contact
Section I - Registration form
Salutation: Prof / Dr / Mr / Mrs / Mdm / Ms, others (please underline)
Name (Please underline surname):
Position / Department / Organization:
Mailing address:
E-mail:
Telephone:
Type of participation (please indicate by X)
Invited speaker ( ) Staff ( ) Student ( ) Sponsor ( ) Exhibitor ( )
Organizing Committee ( )
Preferred mode of presentation (please indicate by X)
Oral presentation (limited places) OR poster presentation ( )
Poster presentation ( )
Attendance only (Not presenting) ( )
Section II Other details:
Dietary preference / requirements and any special needs (please indicate by X):
Vegetarian ( ) Non-vegetarian (Halal) ( ) Any special needs ( )
Section III – Instructions for Abstracts, Oral presentations and Poster Presentations
- Abstract submission deadline is: 1st June 2017
- Acceptance of abstracts is at the discretion of the editors of SNA Symposium 2017.
- The first author is the presenting author of the oral presentation or poster.
- The first (presenting) author of accepted abstracts will be notified by e-mail from 15th June 2017
- The time allotted for oral presentations is: 15 minutes (12 min presentation plus 3 min questions and answers). Presentations to be in Microsoft PowerPoint compatible files, to be pre-loaded into the computer upon registration at venue.
- Poster dimensions: A1 size upright poster (23 X 33 inches)
- Please follow the specifications of the sample abstract (replace sample abstract with your own abstract). The abstract must be limited to 300 words
IMPORTANT: Presenting (first) author’s contact details
Name:
E-mail address:
Telephone:
Mobile number:
Any special requirements:
SAMPLE ABSTRACT (not intended for publication)
1Generation of dopaminergic neurons from induced pluripotent stem cells using B-lymphocytes2G.FLIGHT, M. KAUNT, T.WEASLE
3National University of Singapore, Singapore, Singapore
Objectives: The study aimed at generating patient-specific pluripotent stem cells as it has the potential to accelerate the implementation of stem cells for clinical treatment of neurodegenerative diseases like Parkinson’s.
Methods: B-lymphocytes isolated from spleens of rats were reprogrammed for generation of induced pluripotent stem cells (iPSCs)by inducing expression of the transcription factors KLF4, OCT4, SOX2, and C-MYC. The expression of the transcription factors in the reprogrammed cells was analysed by reverse transcription-polymerase chain reaction (RT-PCR). The resultant iPSCs lines were subjected to the differentiation medium to obtain neural stem cells (NSCs). NSCs differentiated into dopaminergic neurons were examined for immunocytochemical expression of TH marker.
Results: Agarose gel analysis following RT-PCR revealed distinct expression of KLF4, OCT4, SOX2, and C-MYC. By Immunocytochemistry the NSCs generated from iPSCs were found to express neural and glial markers such as Nestin, GFAP and OCT4. Also, the NSCs differentiated into dopaminergic neurons were found to be TH positive.
Conclusion: These results suggest any somatic cell could be reprogrammed to a iPSC and these iPSCs could have a clinical implication in degenerative diseases
4This study was supported by NMRC research grant no: 123-543-289-762
1Title
2Authors (capitalize all letters – omit degrees)
3Institution
4Name of Supporting Agency and Grant Number
The abstract must be limited to 300 words.