The expression pattern of the genes engrailed, pax6, otd and six3 with special respect to head and eye development inEuperipatoides kanangrensis Reid 1996 (Onychophora: Peripatopsidae)
Bo Joakim Eriksson1, Leyli Samadi, Axel Schmid
Department of Neurobiology, University of Vienna, Althanstrasse 14, 1090 Wien, Austria
1. Corresponding author:
Fig. S1.Sequence alignment of Pax6 genes within bilaterians.(A) Paired domain: comparison of amino acid sequence of paired domains and adjacent residues of Pax-6from severalbilaterians. Extent of β-hairpins and α-helices of paired domains are boxed and the secondary structure of the domains is shown at the bottom of the figure. Dots indicate identities in amino acids with the Homo sapiensPax6 sequence shown at the top. The conserved undecapeptide C-terminal of the paired domain is underlined in the Homo sapiens sequence. Gaps or missing data in the alignment are marked by dashes. (B) Homeodomain: comparison of amino acidsequences of homeodomains of Pax-6 of bilaterians.The three α-helices are boxed. Abbreviation of the species names can be found in the table S1.Pax6 is strongly conserved at the primary sequence level, particularlyin the area of the DNA-binding paired and homeodomains (Fig. S1 A and B). A less degree of sequence conservationcan be observed in the linkerregion between the two DNA-binding domains (Fig. S1 residues 130 to 155).However, inspectionof the various Pax6 C-terminal domains revealed that there are several stretchesof conserveddomains within the vertebrates Pax6 (these excludes invertebrate Deuterostome). According to Callaerts et al. (2006), there is a short octapeptide sequence at C-terminal shared bytoy andPax6 proteins, but not by ey. The data is missing in our partial sequence of Ek-Pax6. Therefore, it was not possible to decide whether Ek-Pax6 is homologous toarthropod’s ey or toy according to C-terminal conservation region.This was,therefore, achieved by comparison of functionally andevolutionarily important residues in the paired domain. A comparison of ey and toyhad revealeddifferences in DNA-binding affinity of the paired domain(Czerny et al., 1999). This difference between bounding efficiency of eyand toy to mouse-Pax6 target-DNA had been traced to a single aminoacid difference at position 14 of the paired domain(Czerny et al., 1999). At this position, Ek-Pax6 (shown by the black arrow at the left side of the figures) displays an asparagine (N) which is similar to that of Pax6 and toyin other bilaterians(red arrow marking the position 14). However, a glycine (G) residue is found in the ey genes of Anophelesand Drosophila and a Serine (S) in the eyofTriboliumat the same position.
Table S1List of the species names, their class and phylum, and their abbreviation, the gene names and the GenBank accessionnumbers used in phylogenetic analysis
Abbreviation / Accession Info / Species name / Phylum / ClassAga_ey / EAA06761 / Anopheles gambiae / Arthropoda / Hexapoda
Ame_ey
Ame_toy
Ame_gsbA
Ame_gsbB / XP395177.2
XP394648.3
XP_394847.3
XP_394848 / Apismellifera / Arthropoda / Hexapoda
Bfl_Pax6 / CAA11368 / Branchiostomafloridae / Chordata / Cephalochordata
Bma_Pax6
Bma_prd
Bma_gsb / XM_001898985
XP_001900160
XM_001900123 / Brugiamalayi / Nematoda / Chromadorea
Cco_Pax6 / S37689 / Coturnixcoturnix / Chordata / Vertebrata
Cin_Pax6 / BAB85207 / Cionaintestinalis / Chordata / Tunicata
Cel_Pax6
Cel_Pax3 / NP_001024570
NP_496189 / Caenorhabditiselegans / Nematoda / Chromadorea
Dme_toy
Dmel_ey
Dme_prdA
Dme_prdB
Dme_gsb
Dme_gsbn / CG11186
AAX52512.1
NP_723721.1
NP_523556.1
AAF47315
AAL49215 / Drosophila melanogaster / Arthropoda / Hexapoda
Dps-ey / GA13138 / Drosophila pseudoobscura / Arthropoda / Hexapoda
Dre_Pax6
Dre_Pax3a
Dre_Pax3b
Dre_Pax7a / CAA44867
AAH76069
ACN88554
AAI63580 / Daniorerio / Chordata / Vertebrata
Gga_Pax6 / NP990397 / Gallus gallus / Chordata / Vertebrata
Gma_Pax6.1
Gma_ Pax6.2 / CAE30301 CAE30302 / Glomerismarginata / Arthropoda / Myriapoda
Gti_Pax6 / CAA09227 / Girardiatigrina / Platyhelminthes / Turbellaria
Hsa_ Pax6
Hsa_Pax3 / P26367
AAI14364.1 / Homo sapiens / Chordata / Vertebrata
Lsa_Pax6 / CAA64847 / Lineussanguineus / Nemertea / Anopla
Lpo_Pax6 / EU673470 / Limulus polyphemu / Arthropoda / Chelicerata
Lop_Pax6 / AAB40616 / Loligoopalescens / Mollusca / Cephalopoda
Mmu_Pax6
Mmu_Pax3
Mmu_Pax7 / P63015
AAH48699.1
NP_035169 / Musmusculus / Chordata / Vertebrata
Pdu_Pax6 / CAJ40659 / Platynereis dumerilii / Annelida / Polychaeta
Sdr_Pax6 / ABB52751 / Strongylocentrotusdroebachiensis / Echinodermata / Eleutherozoa
Tca_ey
Tca_Toy
Tca_prdA
Tca_prd B
Tca_gsbA
Tca_gsbB / EFA02894 EFA02830 NP_001071090 EFA05752.1
XM_969092
XM_969119 / Triboliumcastaneum / Arthropoda / Hexapoda
Xla_Pax6 / P55864 / Xenopuslaevis / Chordata / Vertebrata