Atcw (1) ------MTKEVCSNIGLWLLLTLLIGNYV
Atvac (1) -MASTEALLPVTSLQDPLSESRSDQIPETRRRRPIKVHLAVYSGLLLIALYVTLIVTHDG
GU252170 (1) MEASSSTSHDPALFHAPLLYHP-----RRRSSRPLKGFAVIIGSVVFLLSLVTLIVNQSP
FJ864677 (1) MEASYDPEQNPP-LHAPLLDR------SSPRTRRFAAIFACLVSLLLLLALIINQAQ
Atcw (24) VNLEAS------HHVYKRLTQSTNTK------SPS------VNQPYR
Atvac (60) SKAEIATE------SRPR--MAGVSEKSNDGVWISSDDGKVEAFPWNNTILSWQR
GU252170 (56) EPLASNPSSVTEAGSYSMAAQPRGIAEGVSAKSNP------SLFDKVGFNWTNAMFYWQR
FJ864677 (51) QPFEK------VQPRGVAEGVSAKSNP------SLLNQVPFNWTNAMFSWQR
^ < ^
Atcw (53) TGFHFQPPKNWMNDPNGPMIYKGIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPA
Atvac (107) TAFHFQPEKNWMNDPNGPLFYKGWYHFFYQYNPNAAVWGDIVWGHAVSKDLIHWLYLPIA
GU252170 (110) TAYHFQPQKNWMNDPDGPLYHKGWYHLFYQYNPDSAIWGNITWGHAVSTDLIHWFYLPLA
FJ864677 (91) SAYHFQPQKNWMNDPNGPLYHKGWYHLFYQYNPYSAIWGNITWGHAVSRDLIHWLYLPLA
^
Atcw (113) IFPSAPFDINGCWSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSP
Atvac (167) MVPDQWYDANGVWTGSATFLDDGSIVMLYTGSTDEFVQVQNLAYPEDPSDPLLLKWVKFS
GU252170 (170) MVPDQWYDINGCWTGSATLLPDGRIVMLYTGSTNDSVQVQNLAYPANLSDPLLLQWLKYP
FJ864677 (151) LVPDHWYDIKGVWTGSATILADGQIIMLYTGETNESVQVQNLAYPANVSDPLLLHWLKYP
<
Atcw (173) LNPLMAPDAVNGINASSFRDPTTAWLGQDKKWRVIIGSKIH-RRGLAITYTSKDFLKWEK
Atvac (227) GNPVLVP--PPGIGAKDFRDPTTAWKTSSGKWRITIGSKIN-RTGISLIYDTTDFKTYEK
GU252170 (230) GNPVVVP--PTGIEDEEFRDPTTAWLGPDGSWRIVVGTRFNTTIGTALVFQTTNFSDYEL
FJ864677 (211) GNPVMVP--PPGVKPDDFRDPTTAWLGPDGTWRLTMGSKFDTTIGISLVYHTTNFRDYEL
< < <
Atcw (232) SPEPLHYDDGSGMWECPDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTI
Atvac (284) HETLLHQVPNTGMWECVDFYPVSKTQLNGLDTSVNGPD---VKHVIKASMDDTRIDHYAI
GU252170 (288) LDGVLHAVPGTGMWECVDFYPVAINGSVGLDTTALGPG---IKHVLKASLDDTKVDHYAI
FJ864677 (269) LDGVLHAVPGTGMWECVDFYPVAINGSVALDTSSLGPG---IKHVLKASLDNTKVDHYAI
Atcw (292) GTYDRVKDKFVPDNGFKMDGTAP--RYDYGKYYASKTFFDSAKNRRILWGWTNESSSVED
Atvac (341) GTYDDSNATWVPDNPSIDVGISTGLRYDYGKYYASKTFYDQNKGRRILWGWIGESDSEAA
GU252170 (345) GTYDMITDKWTPDNPEEDVGIG--LKVDYGRYYASKTFFDQSKQRRILYGWVNETDSEAD
FJ864677 (326) GTYDPITDKWTPDNPEEDVGIG--LKVDYGRYYASKTFFDQHKQRRVLWGWINETDTETA
Atcw (350) DVEKGWSGIQTIPRKIWLDR-SGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYG
Atvac (401) DVQKGWSSVQGIPRTVVLDTRTHKNLVQWPVEEIKSLRLSSKK-FDMTIG-PGTVVPVDV
GU252170 (403) DLEKGWASIQTIPRSVLYDNKTGTHLLQWPVEEVESLRLNATV-FKDVVVEAGSVVPLDI
FJ864677 (384) DLKKGWASLQTIPRTVLYDNKTGTNLLQWPVEEVESLRLNSTM-FKEVLVEPGSVVPLDI
Atcw (409) VTAAQADVEVLFKVRDLEKADVIEPSWTDPQLICS---KMNVSVKSGLGPFGLMVLASKN
Atvac (459) GSATQLDIEAEFEIKTDDLKLFFDDDSVEADNKFSCETNGGSTARGALGPFGFSVLADEG
GU252170 (462) GTATQLDILAEFEIETLVLNSTEDEVSDCGD------GAVDRSTYGPFGVLVIADDS
FJ864677 (443) GTTTQLDILAEFEIEPLIP-STTNEIDNCGD------GAVDRSTYGPFGLLVIADAS
Atcw (466) LEEYTSVYFRIFKARQNSNKYVVLMCSDQSRSSLKEDNDKTTYGAFVDINPHQPLSLRAL
Atvac (519) LSEQTPVYFYVTKGKHS--KLNTVFCTDTSRSTLANDVVKPIYGSFVPVLKGEKLTMRIL
GU252170 (513) LSELTPIYFRPLNTSDG--SLETYFCADETRSSKAPDVTKRVYGGKIPVLDDENYNMRVL
FJ864677 (493) LSELTPIYFRPLNASDG--SLKTYFCSDETRSSKASDVFKQVYGGKVPVLDDENYNMRVL
Atcw (526) IDHSVVESFGGKGRACITSRVYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMNSAQIS-
Atvac (577) VDHSIVEGFAQGGRSCITSRVYPTKAIYGATKLFLFNNAIDATVTASFTVWQMNNAFIHP
GU252170 (571) VDHSVVESFGGGGRTVITSRVYPTEAIYGAARLFLFNNASGVNVKATLKIWEMNSAFIRP
FJ864677 (551) VDHSIVESFAQGGRTVISSRIYPTEAIYGAARLFLFNNATGVNVKATLKIWELNSAFIRP
Atcw (585) ------
Atvac (637) YSSDDLGVPSST---
GU252170 (631) FPFEETLFQEMVAST
FJ864677 (611) FPFERDSV------
Supplementary data 1. Alignment of Arabidopsis cell-wall invertase, Arabidopsis vacuolar invertase with two cotton vacuolar invertases. Amino acids marked with < are involved in substrate binding and amino acids marked with a ^ are tryptophanes involved in the active site. The putative cleavage sites for the leader sequence are underlined.