GROUP – A

Advanced Science & Technology Research Centre,VBU

Sl. No. / Items Name & Specification
Flow cytometer (NXT) Fluorescent Microscope ( Objective- 10x, 20x, 40x, 100x),
With blue, Green and Red light tube.
Specification: -
  • Instrument should be a table top model, using acoustic focusing which can align cells for better focusing to detect rare events even at higher flow rate.
  • Instrument should be able to detect at least eight parameters inclusive of two scatters.
  • Instrument should be available with violet+blue (405nm/488nm) or red+blue (638nm/488nm) combinations and should be upgradable upto 4 lasers and sample automation capabilities.
  • Laser profile: Flat top laser requiring minimal alignment
  • Instrument should be able to provide 6 decades of resolution and should be able to capture capture at least 35,000 events/sec at high flow rates.
  • Instrument should be able to provide absolute counts without the need for counting beads.
  • Instrument should be able to Detects particles and cells from 0.5 μm to 50 μm.
  • Forward scatter: Photodiode detector with 488/10 bandpass filter
  • Side scatter: PMT with 488/10 bandpass filter
  • Instrument should have very low fluid consumption (about 1.8 L/day). Fluid storage should be possible within instrument.
  • Instrument should have user selectable sample flow rates ranging from 12.5–1,000 μL/min.
  • Instrument hardware and software should allow user adjustable voltage and threshold setting of all parameters. It should allow both automated and manual compensation settings.
  • The software should allow automated start up, performance tracking and shut down capability within 15 mins
  • Sample input rate should be equivalent or more than one sample per minute.
  • Sample loading volume should be similar to 20 μL–4 mL range.
  • Instrument should have fluid storage within instrument with level sensing along with external tank option
  • Instrument should have user friendly software with visual display of system status on board. The software should have the capability to handle data files of upto 35 million events/file and export to standard FCS v3.1 format.
  • Instrument should be small and portable to fit on standard lab bench or under laminar hood.
Laser / Excitation / Emission / Common dye / Fluorescent protein
Violet / 405 nm / 440/50 / Pacific BlueTM / ECFP
512/25 / Pacific GreenTM
603/48 / Pacific OrangeTM
710/50 / Qdot 705
Blue / 488nm / 488/10 / FSC
488/10 / SSC***
530/30 / FITC / EGFP, Emerald GFP
574/26* / Propidium Iodide / EYFP
590/40** / Propiduim iodide Fluorescent proteins / EYFP
695/40 / Per CP-Cy 5.5
780/60 / PE-Cy 7, Qdot 800
Yellow / 561nm / 585/16 / PE / RFP
620/15 / PE- Texas Red / Mcherry, dtomato, Ds Red, m Straberry
695/40 / PE-cy 5.5
780/60 / PE-Cy 7
Red / 637nm / 670/14 / APC
720/30 / Alexa Fluor 700
780/60 / APC- Alexa Fluor 750
Dynamic Light Scatterer (Zeta sizer)
Specification: -
Measurement Facility -
a)ParticleSizeRange – 0.3nm to 5000 nm
b)Zeta Potential and mobility – No Practical Limit
c)Molecular weight range – 1000Da to 2 x 107 Da
Particle Size:
ParticleSizeRange (Dia) : 0.3nm to 5 microns
Particle Size Detection Resolution : 3 decimal
Measurement Technique : Dynamic Light scattering
Purge facility (For Condensation control) : Using Dry Air/Nitrogen
Correlator No. : 4000
Correlator Type b : High Sped Digital Correlator
Control : Through Software
Speed : 1ns
Temperature range : 00C – 900C
Temperature resolution : +/- 0.10C
Sample Cell Temp. Controller : Peltier Type
Laser power : 4 mW
Laser Type : Stable Visible Laser
Laser Class : Class 1 Compliant, EN60825-1:2001 and CDRH
Zeta Potential:
Principal : Laser Doppler Velocimetry and Micro Electrophoresis
Measuring Technique : M3 – PALS (Phase Analysis Light Scattering)
pH range : 2 to 13
Size range for Zeta Potential : 3.8 nm to 100 micron
MobilityRange : Min. zero to no effective maximum
Conductivity range : 0 to 200 mS/cm
ZetaPotentialRange : No effective limitations
Minimum Sample Volume : 750uL folded capillary cell
Measurement : Totally Automated
Attenuation : Totally Automated
Display Result : Zeta Potential (mV), Mobility (ucm/Vs) Conductivity (mS/cm)
& Temperature (oC)
Detector : Avalanche Photodiode
Cell Type : Maintenance & Contamination free Folded Capillary Electrophoresis cell
Cell Recognition : Automated
Computer Interface : Very high speed USB
Operating Software : Windows operating system
Software supports : SOP (Standard Operating Procedure) only compatible with
Windows operating systems
Molecular Weight:
Molecular weight range 342 Da to 2 x 107Da**
(Estimated from Hrdrodynamic diameter)
Measurement Technique Static Light Scattering
Minimum Sample Volume 20ul
Optics unit with internal 4m W, High performance, Stable, Long life Visible laser. System conforms to the requirements of CDRH Class 1 and BS EN60825 Class 1.
Meterials in contact with sample: PTFE, silicone, polycarbonate, polypropylene, gold
Installation kit comprising:
Software on CD
Manual
Mains cable
USB cable
Application Software:
  • Compatible with Windows operating systems
  • Fully automated operation.
  • Standard Operating Procedure (SOPs)- for repeatability between operators system and site.
  • Custom Report Generation- to meet every laboratory’s requirement.
  • Built-in library for Refractive Index.
  • Intensity wise Particle Size Distribution and Statistics.
  • Number wise Particle Size Distribution and Statistics
  • Correlation function plot.
  • Crystal Screening.
  • Over plotting of result for direct comparison.
  • Full range of statistical plots
  • Temperature trend facility
  • Time & Temp Scan of Bio-molecular Size Distribution.
  • 2nd Viral Co-efficient determination.
  • Protein Melting Point Determination.
  • Protein and Polymer Characterization.
  • Formulation Stability
  • Display of Zeta Potential, Mobility, Conductivity, Temp, ISO Electric Point
  • Zeta Potential distribution
  • Electrophoretic mobility and its distribution
  • Zeta Potential Vs pH
  • Electrophoretic mobility vs pH
  • Zeta Potential Voltage vs Current
  • Phase plot
  • Frequency Shift
  • ISO-Electric point
  • Emulsion Stability.

Ultracentrifuge
Specification: -
  • Microprocessor controlled floor model ultracentrifuge with Color touch-sensitive LCD and GUI (graphic user interface) with high contrast against screen in black display.
  • Quick Start-Up with-in 8 to 10 second to be ready for operation since power switch is turned on.
  • Max Speed should be 1,50,000 RPM (with-in 90 second) or better.
  • MAX G Force should be 10,50,000 x g or better
  • Speed control accuracy ±50 (5,000 to max. speed)
  • Max capacity 30ml x 6 tubes
  • Timer : 1 min. to 99hrs. and 59 min. HOLD and RTC (real-time control) function.
  • Oil rotary vacuum pump and oil diffusion pump
  • Temperature 0 to + 40 ºC ( 1 degree increment)
  • Thermo-module cooling system (no special tool required)
  • Quick operating sound 45 dB(A)
  • Samples need to be balanced within 5mm by visual check only. Non-contact imbalance sensor always monitors vibration of the rotor and drive shaft. Incase of abnormal vibration, the sensor activates and stops operation immediately.
  • Max. heat dissipation into room 0.7 kW or less
  • Drive unit warranty at least 5 years
  • Dimensions (mm) 440 (W) x 52 (D) x 910 (H), height to table 790 or similar
  • Weight approx 100-105 kg
  • IEC61010-2-020, EN61326, CE marking qualified
  • In order to prevent for exhausting biohazardous sample to a room, micro filter can be assembled in a vacuum line as a option (Factory installed)
  • System should be completed with following rotors:
  • Fixed angel rotor 8 x 13.5 ml with 100 pcs tubes
  • Fixed angel rotor 6 x 30 ml with 100 nos.

Real Time PCR
Block Format / 3x32- well, 0.2 ml independent control / 96-well, 0.2 ml
Veriflextm Blocks / 2 X 96- well, 0.2 ml / 2 x flat Block for Open Array plates
Features / Run three experiments at once / Perform complete optimization work with full 96-well VeriflexTM Blocks / High – throughput in 96-well format / Highest throughput capability 16 X 1532
Max block ramp rate / 6.00ºC/sec / 6.00ºC/sec / 2.0 ºC/sec / 2.0 ºC/sec
Max ample ramp rate / 4.40ºC/sec / 4.40ºC/sec / 1.6ºC/sec / 1.6ºC/sec
Temperature accuracy / ±0.25ºC (35-99.9ºC)
TemperatureRange / 0-100.0ºC
Dimensions / Height: 27.2 cm (10.6 in)
Width: 33.0 cm (13 in)
Depth: 56.5 cm (22 in)
Weight / 18.75 kg (41 lb)
PCR volume range / 10-80µL / 10-80µL / 5-20 µL / 33 nL
Instrument memory / USB, on-board
Display interface / 8.4 in. color TFT LCD
Power / 100-240 V, 0-60 Hz, Max: 950 VA
VeriFlexTM Blocks / 5ºC, 2 VeriFlextm zones / 25ºC (5ºC zone-to-zone),6 VeriFlexTM / N/A / N/A
Cat. No.: Complete system (base and block) / 4484073 / 4484075 / 4484076 / 4484078
Cat. No.: Block n(includes block only) / 4483638 / 4483637 / 4484071 / 4484074
Sonicator
Specification: -
  • Ultrasonic processor, for manual/stand operation suitable for volume up to 250 ml.
  • Output: 500 Watts, frequency: 30 kHz
  • Automatic frequency & adjustable pulse tuning system
  • Variable amplitude should be dry running protected. In portable case with mounting Tools.
  • Sonotrode, made of titanium, for samples 2 ml to 50 ml. Removable tip type sonotrode will not be accepted.
  • Timer from 0 to 99 mins. 59 sec.
  • Should be supplied with storage box & mounting tools.

Workstation
Technical Specification: -
Specification / Description
System board Form Factor / 33.02 x 36.19 cm (13 x 14.25 in) Custom Form Factor
Processor socket / Dual LGA2011
CPU bus speed / QPI: Up to 8.0 GT/s
Super I/O controller / Nuvoton NPCD379H
Memory expansion slots / 16 slots (8 slots per CPU)
Memory type supported / DDR3, RDIMM (Registered) or UDIMM (Unbuffered), ECC, LR (Load Reduction) DIMMs
Memory modes / NUMA (Non-Uniform Memory Architecture), Memory Node Interleave
Memory speed supported / 1066-MHz, 1333-MHz, & 1600-MHz
Interfaces supported / 2-channel SATA 6.0 Gb/s Interface (2 channels e-SATA configurable)
4-channel SATA 3.0 Gb/s Interface
8-channel 6Gb SAS interface (8 SAS connectors on the motherboard), SAS ports can be ported externally by using the SAS Bulkhead and/or Back Panel connector Kits
USB 3.0, USB 2.0, IEEE 1394a
Maximum memory / Supports up to 64GB using UDIMMs
Supports up to 256GB using RDIMMs
Supports up to 512GB using LRDIMMs