Supplementary material for Terblanche et al.

Table S1 Effects of gender, feeding status and age (24, 240, 288 or 576 h) on CTMax and CTMin in laboratory-reared Glossina pallidipes. (Experimental protocol: Start temperature = 16°C (CTMin) or 35°C (CTMax); rate of temperature change = 0.25°C/min)

Effect / DF / F / P
CTMax / Gender / 1 / 0.000 / 0.884
Feeding / 1 / 0.000 / 0.738
Age / 3 / 1.0 / 0.631
Error / 120
CTMin / Gender / 1 / 0.000 / 0.983
Feeding / 1 / 0.408 / 0.524
Age / 3 / 0.526 / 0.665
Error / 170


Table S2 Summary of annual climate data for Mfuwe, Zambia taken from interpolated weather station data using Diva-GIS software over 1950-2000 (for details on interpolation methods and climatic variables see Hijmans et al., 2005). Tmin: minimum temperature within the month; Tmax: maximum temperature within the month.

Tmin
(°C) / Tmax
(°C) / Rainfall (mm)
January / 20.1 / 30.6 / 220
February / 20.0 / 30.3 / 195
March / 19.5 / 30.7 / 130
April / 17.7 / 30.4 / 40
May / 14.2 / 29.2 / 3
June / 11.2 / 27.6 / 0
July / 10.8 / 27.8 / 0
August / 12.7 / 29.8 / 0
September / 16.2 / 33.5 / 0
October / 19.4 / 35.5 / 13
November / 20.6 / 34.8 / 86
December / 20.3 / 31.5 / 178
Annual / 16.9 / 31 / 865

Figure S1 Experimental thermocouple (Type T 40-SWG) recordings from cooling trials (0.25°C/min; 20°C starting temperature) for Glossina pallidipes (n = 6) body temperatures (Tb) and the chamber temperature (Tc, n = 2) demonstrating that flies equilibrate rapidly to changing temperature when taken from ambient conditions. Furthermore, a lag in body temperature behind chamber temperature did not occur during the trials with the fastest rates of temperature change.

References

Hijmans, R.J., Cameron, S.E., Parra, J.L., Jones, P.G., Jarvis, A., 2005. Very high resolution interpolated

climate surfaces for global land areas. International Journal of Climatology 25, 1965-1978.