RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES
KARNATAKA, BANGALORE
SYNOPSIS
BY
Dr. ASHA P.
TITLE OF TOPIC
“EVALUATION OF HYPOGLYCAEMIC ACTIVITY OF
RUDANTI (CAPPARIS MOONI WIGHT.) AN EXPERIMENTAL STUDY”
2012-2013
SHRI.J.G.C.H.SOCIETY’S
AYURVEDIC MEDICAL COLLEGE, GHATAPRABHA,
BELGAUM.
FROM
DR. ASHA .P.
I MD
Dept.of.Dravyaguna
Shri.J.G.C.H.S, Ayurvedic Medical College
Ghataprabha,Belgaum.
TO,
THE REGISTRAR
Rajiv Gandhi University of Health
Sciences, , Bangalore ,Karnataka
Jayanagar, 4th block,Bangalore
THROUGH,
THE PRINCIPAL AND HOD OF DRAVYAGUNA
Shri.J.G.C.H.S, Ayurvedic Medical College,
Ghataprabha, Belgaum.
Sub: Submission of synopsis for registration of subject for dissertation.
Respected Sir,
I request you to kindly register the below mentioned subject against my name for the submission of dissertation to the Rajiv Gandhi University of health sciences, Karnataka, Bangalore for the partial fulfilment of post graduation course in the specialization of DRAVYA GUNA M.D.(AYU).
TITLE OF DESSERTATION
“EVALUATION OF HYPOGLYCAEMIC ACTIVITY OF
RUDANTI (CAPPARIS MOONI WIGHT.) AN EXPERIMENTAL STUDY”
I am enclosing completed Proforma for registration of subject for dissertation.
Thanking You,
Yours faithfully
PLACE: GHATAPRABHA
DATE:
(Dr. ASHA.P.)
RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES,
KARNATAKA, BANGALORE
ANNEXURE II
COMPLETED PROFORMA FOR REGISTRATION OF SUBJECT FOR
DISSERTATION
1 NAME OF THE CANDIDATE: Dr.ASHA.P.
AND ADDRESS I Yr. M.D. (Ayu)
W/O.L.R.RAHAVENDRAN
NO 128,3RD CROSS,
CHENNAMMANAKERE, ACHUKATTU
BSK 3RD STAGE, BANGALORE-560085
KARNATAKA
2. NAME OF THE INSTITUTE : Shri J.G.C.H.Society’s
Ayurvedic Medical College
Ghataprabha.
3 .COURSE OF STUDY : Ayurveda Vachaspathi M.D (Ayu)
(DRAVYA GUNA)
4. DATE OF ADMISSION OF COURSE : 11/10/2012
5. TITLE OF THE TOPIC: “EVALUATION OF HYPOGLYCAEMIC ACTIVITY OF
RUDANTI (CAPPARIS MOONI WIGHT.) AN EXPERIMENTAL STUDY”
6 BRIEF RESUME OF THE INTENDED WORK:
6.1 Need for study-
Diabetes is a clinical syndrome where in hyperglycaemia is one of the diagnostic criteria. The root causes of the disease are change in life style, stress and other environmental factors along with endocrinal derangement. The disease is so prevalent that in near future, India it is supposed to have half of the population suffering from this disease entity. Worldwide 3.2 million diabetes-related deaths are reported annually, a number equivalent to that of HIV/AIDS-related deaths, one in every 20 deaths is attributed to diabetes equating to 8,700 deaths per day, or 6 deaths every minute. Based on 2005 figures, at least 171 million people worldwide have diabetes. This number is expected to double by the year 2030. In this regard a multidimensional approach is necessary to arrest the disease process.
Even though the modern drugs are taking care of hyperglycaemia, but it is not sufficient as they are not arresting the disease progress rather complications effects of the same are seen along with beneficial effects. These medicines are having such a threatening side effect as to cause Hypoglycaemia, which if not treated early patient may succumb to death.
Traditional medicines are gaining popularity in the treatment of diabetes and its complication due to their efficacy, minimal side effects and low cost. The world health organization has also recommended the evaluation of plant effectiveness in conditions where we lack safe modern drugs. This has lead to an increase in the demand of research on anti diabetic natural products which produce minimal or no side effects.
Scientists are doing day to day research and discovering newer chemicals but not able to lessen their side effects, Medicinal plants, which form the backbone of traditional medicine, have in the last few decades has been the subject for very intense pharmacological studies; this has been brought about by the acknowledgement of the value of medicinal plants as potential sources of new compounds of therapeutic value.
. Capparis mooni wight is drug referred by P.V.Sharmaji in vol 5 as Rudanti.1 Capparis mooni Wight, belonging to capparidaceae family commonly known as Rudanti is a shrub which is used traditionally in asthma, bronchitis, dyspepsia, diabetes, aphrodisiac and general debility etc. The objective of present investigation is to study the effect of Rudanti (Capparis mooni Wight) on blood glucose in normal and diabetic induced rats.
Ayurveda is one of the safest and effective systems of medicine, where many single herbs having anti-diabetic properties have been mentioned. Today much priority is given to herbal medicine especially in treatment of chronic disease syndromes. To quote a few Amalaki, Haridra, Jambu, Meshashringi, Karavellaka, Bilwa, Nimba have got promising results in treating Hyperglycaemia.
The condition of hyperglycaemia coincides with the concept of Prameha in Ayurveda. This disease is related to Kapha and Kleda. To reduce Kapha and Kleda the Tikta, Katu, Kashaya, Ruksha, Ushna properties are useful. The drug Rudanti has got Rasa-Tikta, Kashaya Vipaka- Katu, and Guna- tikshna, Laghu2, Virya- Ushna and Karma-Kaphavatashamaka. Hence according to Guna- Karma it will be useful in Prameha. Capparis mooni Wight. Is drug referred by Narahari pandit in Raja Nighantu as Mehari3. C.P. Khare in Indian Medicinal Plants has mentioned that Rudanti Phala churna is used in Diabetes and Diabetic ulcers4.
Hence present study is undertaken to give an economical, cost effective, scientific approach in reconfirmation of the hypoglycemic effect of Rudanti (Capparis mooni Wight.).
6.2 Review of Literature:
1. Literature data of Diabetes Mellitus will be collected from all available modern literatures and Ayurvedic literatures.
2. The data of drug Rudanti (Capparis Moonii Wight) will be collected from Nighantus, present Ayurvedic literature, journals and internet.
PREVIOUS WORK DONE
1. Rath K.S.- A Pharmaco therapeutic study of neem oil on madhu meha, IPGT &RA Gujarat Ayurvedic University Jamnagar-1998
2. Pandey M.C.-Study on Hypoglycemic effect of bark of Pterocarpus marsupium (Beejaka) - Faculty of Ayurveda Institute of Medical Sciences BHU Varnasi-1975.
3. Joshi.V.K.-A study on the hypoglycemic effect of Clerodenron infortunatum Linn- Faculty of Ayurveda Institute of Medical Sciences BHU Varnasi-1978
4. Tissera M.H.A- Hypoglycemic effect of Salacia reticulata Wight (Indigenous drug of Srilanka) - Faculty of Ayurveda Institute of Medical Sciences BHU Varnasi-1996.
5. Sarza Monsy Ooman- An experimental study on effect of Bhunimba in Prameha-Government Ayurvedic College Thiruvanantapuram-1995
6. Priya.S- An experimental study on effect of Aranyajeeraka in Madhumeha- Government Ayurvedic College Thiruvanantapuram-1998.
7. Shwetha Kulkarni-“An experimental study on Kshudragnimantha (Clerondendrum Phlomidis Linn.) with special reference to its hypoglycemic activity.”2010
8. Sachin Javali -“Experimental Evaluation of Hypoglycemic effect of Aragwadha (Cassia Fistula Linn.) in streptozotocin induced diabetic rats”2012.
9 Krup.s-“A Clinical study on Madhumehahara (Anti Hyperglycaemic) Effect of bimbi mula [coccinia grandis.]2012.
10 Ann Mary-‘‘Effectiveness of fenugreek on glycemic level among Diabetic elderly in selected old age homes, Mangalore’’2011.
6.3 Objectives of study:
1. Complete pharmacognostical study of (Morphological and microscopical study) of Rudanti (Capparis mooni Wight.).
2. Preliminary Phytochemical analysis of Rudanti (Capparis mooni Wight).
3. To assess the hypoglycemic effect of Rudanti (Capparis mooni Wight)
7. Materials and Methods
7.1 Source of data
a) Literary source of data
The literary source required for the present study will be done through various classical texts like Nighantu, latest journals, websites, relevant research work etc.
b) Pharmacognostical source of data
The pharmacognostical source of data will be done through organoleptic and microscopical study.
c) Phytochemical source of data
The data of Preliminary phytochemical analysis and pharmacognostic analysis will be collected out by carrying out the respective tests in Dravyaguna Laboratory of Shri.J.G.C.H.S.Ayurvedic Medical College Ghataprabha.
Experimental source of data
The experimental study will be carried on healthy albino rats of either sex. Data will be generated by conducting the hypoglycaemic activity and data are collected
7.2 Methods of collection of data:
For present study the Rudanti phala (Capparis mooni Wight) will be collected from the market and will be authenticated by a botanist.
Phala churna of Rudanti (Capparis mooni Wight) will be extracted with soluble solvent in soxhalet apparatus. The obtained extract will be subjected for hypoglycemic activity.
The albino rats will be procured from Government approved animal house and the Hyperglycaemia will be induced in them using the Dunn and Mcletchie (1954)5 method.
Grouping
The healthy adult albino rats of either sex weighing from 110 Gms to 180 Gms will be subjected to detailed examination and will be grouped into 3 groups of each having 6 albino rats.
Induction of Diabetes
• Before starting the experiment, animals will be separated according to their body weight. Animals will be injected with Alloxan monohydarte freshly prepared in normal solution intraperitonially at a dose of 150mg/kg of body weight ; the animal will be tested for evidence of diabetes by estimating their blood glucose level using glucometer.
• Before the administration of drug orally the blood sugar level will be recorded in all the groups.
Groups / Albino rat / Drug / Name / dosageGroup I / 06 / Test drug / Rudanti(phala churna) / 200mg/kg .b.w.
GroupII / 06 / Standard drug / Glibenclamide / 10mg/kg.b.w
GroupIII / 06 / - / Distilled water / -
Formula-Rat dose =Adult human dose x 0.018
Monitoring
• Then the respective drugs will be immediately administered orally in a single dose for Group I, II & III of hyperglycaemia induced albino rats. Then the hypoglycaemic activity will be assessed by observing changes in the blood sugar level by drawing 0.2ml blood from the tail vein in the experimental animals at regular intervals of 2 hours for 1 day.
Assessment:
Assessment will be done by applying obtained data to unpaired students ‘t” test.
7.3 Does the study require any investigations or interventions to be conducted on patients or other humans or animals? If so describe briefly;
The study requires hypoglycemic model for the study of Rudanti (Capparis mooni Wight) with special reference to its hypoglycemic activity.
Hyperglycaemia will be induced on albino rats and these rats are maintained on standard circumstances.
7.4 Has ethical clearance been obtained from in case of 7.3?
Yes, clearance has been obtained through the ethical committee formulated in the institution.
8. LIST OF REFERENCES
1. Prof P.V.Sharma, Dravyaguna vijnana Vol. V Rudanti, page no 345,published by Chaukhambha sanskrita pratishthan reprint 2003.
2. Prof P.V.Sharma, Dravyaguna vijnana Vol. II adhyaya 9 Shoshahara, 379- Rudanti, page no 829, published by Chaukhambha sanskrita pratishthan, reprint 2003.
3. Narahari pandit, Raj nighantu Parpatadi varga Rudanti,page no 116-117 edited with “Dravyagunaprakashika” Hindi commentary by DR Indradeo Tripathi, published by Krishna das academy,Varanasi in 1998
4. Indian medicinal plants page no 118, Phala churna used in diabetes and in diabetic ulcer .An illustrated dictionary edited by author C.P.Khare.
5. G.K.Gosh,International Journal of Pharmaceutical Sciences and Research, Vol 4,Page no 1370-1377, ,Issue 4,April 2013.
6. Pharmaconosy reviews (phcog rev) supplement vol 2, issue 4, july-dec 2008 page 36-45,Phytochemical and pharmacological review states that all Indian Capparis species are hypoglycaemic.
9. Signature of the candidate :
10. Remarks of the Guide :
11. Names and Designation of :
(In block letters)
11.1 Guide : DR. K.S.KUCHANUR
MD(AYU) Dravyaguna
Asst Professor,Dept of Dravyaguna
Shri J.G.C.H.S.Ayurvedic Medical College.
Ghataprabha – 591321
Tal-Gokak, Dist-Belgaum. Karnataka
11.2 Signature:
11.3 Co-Guide: DR.SAROJINI.D.BYADAGI
MD(AYU) Dravyaguna
Lecturer. Dravya guna Department.
Shri J.G.C.H.S.Ayurvedic Medical College,
Ghataprabha – 591321
Tal-Gokak, Dist-Belgaum. Karnataka State
11.4 Signature:
11.5 Head of the DR. MAYURESH AGTE
Department: MD(AYU)Dravyaguna
Proff. Dravya guna Department.
Faculty of Post-Graduation
Shri J.G.C.H.S.Ayurvedic Medical College,
Ghataprabha – 591321
Tal-Gokak, Dist-Belgaum. Karnataka State
11.6 Signature:
11.7 Remarks of Chairman:
And Principal.
11.8 Signature: