LINDA A. R. MCCAULEY
Woods Hole Oceanographic Institution, Biology Department, MS #32
Woods Hole, Massachusetts 02543
E-mail:
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CURRENT RESEARCH INTERESTS
The evolution of toxic genes in Alexandrium.
Intrapopulation variation of Alexandrium in the Gulf of Maine.
EDUCATION
Fordham University, Bronx, NY. Graduation: February 2003. Research Advisor: Dr. John Wehr.
M.S. major in Biology, concentrations in Ecology and Molecular Biology
Eckerd College, St. Petersburg, FL. Graduation: May 1999. Research Advisor: Dr. W. Guy Bradley.
B.S. major in Marine Biology, minor in Chemistry
University of Queensland, Brisbane, Queensland, Australia. Spring Semester Abroad 1998.
Research Advisor: Dr. Bernie Degnan.
Bermuda Biological Station for Research, St. George, Bermuda. Summer 1997 and Winter 1998.
Research Advisor: Dr. Hank Trapido-Rosenthal. Independent studies and one course
RESEARCH AND LABORATORY EXPERIENCE
Research Assistant III, Woods Hole Oceanographic Institution, April 2004 – Present.
Work independently on research projects in Don Anderson’s Lab. Research focuses on the study
of harmful algal blooms in the Gulf of Maine. Techniques used include: culturing Alexandrium,
PCR, high through-put sequencing, amplification and analysis of microsatellites, data analysis,
phylogenetic analysis, preparation of manuscripts, review manuscripts and grants.
Research Assistant II, Marine Biological Laboratory. August 2002 – April 2004.
Work independently on numerous research projects as directed by Dr. Fredrick Goetz and Dr. Steven Roberts. Research techniques used regularly include: real time RT-PCR , high through-put sequencing, construction of cDNA libraries, isolation of microsatellites, Northern blots, NU-PAGE, Western blots, cloning, total RNA extraction, mRNA extraction, reverse transcription, PCR, RT-PCR, and DNA extraction. Other responsibilities include: preparation of manuscripts, data analysis, review manuscripts and grants, train interns, produce standard molecular and laboratory reagents (bacterial plates, media, solutions), order lab supplies, keep track of budgets.
Master’s Thesis Research, Fordham University. September 2000 - February 2003.
Thesis title: Phylogenetic relationships of freshwater brown algae (Phaeophyceae) based on Rubisco large subunit and ribosomal DNA sequences. Research involved a molecular and phylogenetic analysis of the 18S rRNA gene and the rbcL gene of freshwater brown algae. Techniques used are culture maintenance, DNA extraction, PCR, high through-put DNA sequencing (at the American Museum of Natural History, NY), and phylogenetic analysis.
Molecular Training, Fordham University. September 2001 – May 2002.
Trained to perform various molecular techniques including: PCR, PCR purification, cloning, manual DNA sequencing, SDS-PAGE, Western blot, RNA extraction, and RT-PCR.
Research Assistant Volunteer, Osborn Labs, NY Aquarium. November 1999 – January 2000.
Participated in research project involving zooxanthellae extraction and isolation. Reviewed journal articles and grants for Dr. Andrew Baker.
Independent Research Project, Molecular Zoology Laboratory, University of Queensland. Spring 1998.
Assisted Ph.D. student, Elizabeth O’Brien, in her research. Performed PCR, ran agarose gels, and analyzed DNA sequences.
Undergraduate Thesis Research, Marine Molecular Physiology Laboratory, Bermuda Biological
Station for Research. Summer 1997 and Winter 1998.
Thesis title: Sequence analysis of a small subunit ribosomal RNA (ssRNA) pseudogene from
symbiotic dinoflagellate algae of the genus Symbiodinium. Research involved coral identification
and collection, zooxanthellae extraction and isolation, DNA extraction, PCR, RFLP, cloning, and manual DNA sequencing.
TEACHING EXPERIENCE
Train Intern, Woods Hole Oceanographic Institution. Summers 2005 and 2006.
Teach intern molecular techniques including: culturing Alexandrium, single cell isolation of
Alexandrium, PCR, running agarose gels, fragment analysis of microsatellites, data analysis,
and making cluster trees.
Train Interns, Marine Biological Laboratory. September 2003 – April 2004.
Teach interns molecular techniques including: PCR, running agarose gels, cloning, isolating microsatellites, mRNA extraction, and making bacterial plates, media, and solutions.
Instructor in Introduction to Biology II, Fordham University. Spring 2002.
Responsibilities included: 15-30 minute lecture at the beginning of each laboratory class (twice a week), supervising and guiding students, writing exams with other graduate students and faculty, grading exams, written reports, and laboratory notebooks.
Teaching Assistant to Introduction to Biology I and II, Fordham University. Fall 2000 – Fall 2001.
Responsibilities included: assisting teaching fellow during laboratory, grading students’ written work, and setting up laboratories.
Mentor, Molecular Lab at Osborn Laboratories, NY Aquarium, NY. November 1999 – January 2000.
Taught zooxanthellae extraction techniques from corals to Master’s student working in the laboratory.
Teaching Assistant in Genetics, Eckerd College. Fall 1998.
Responsibilities included: assisting professor during laboratory, setting up laboratories before
each class, and meeting with students weekly for one on one assistance.
PROFICIENT IN
Word, Word Perfect, Excel, Power Point, Sequencher, MacVector, AssemblyLIGN, MacClade, SigmaStat, PAUP*, EndNote, PhylTools, Phylip, PopGene, FPMiner.
ACADEMIC FELLOWSHIPS AND HONORS
Luce Fellowship, Fordham University. 2000-2002.
Fulbright Fellowship Runner-Up. 1999.
Graduation with Honors, Eckerd College. 1999.
Galbraith Fellowship Research Scholarship, Eckerd College. 1997.
Honors Scholarship, Eckerd College. 1995-1999.
PUBLICATIONS
Biga, PR, Roberts, SB, Iliev, DB, McCauley, LAR, Moon, JS, Collodi, P, Goetz, FW. (2005) The isolation, characterization, and expression of a novel GDF11 gene and a second myostatin form in zebrafish, Danio rerio. Comparative Biochemistry and Physiology Part B, 141: 218-230.
Goetz, FW, Iliev, DB, McCauley, LAR, Liarte, CQ, Tort, LB, Planas, JV, McKenzie, S. (2004)
Analysis of genes isolated from lipopolysaccharide-stimulated rainbow trout (Oncorhynchus mykiss) macrophages. Molecular Immunology, 41: 1199-1210.
Goetz, FW, Norberg, B, McCauley, LAR, Iliev, DB. (2004) Characterizations of the cod (Gadus
morhua) steroidogenic acute regulatory protein (StAR) sheds light on StAR gene structure in fish.
Comparative Biochemistry and Physiology Part B, 137: 351-362.
Goetz, FW, McCauley, L, Norberg, B. (2005) Using global genome approaches to address problems in
cod mariculture. ICES Journal of Marine Science 63: 393-399.
McCauley, LAR, Wehr, JD. (2006) Phylogenetic relationships of freshwater brown algae
(Phaeophyceae) based on Rubisco large subunit and ribosomal DNA sequences. Phycologia. In review
McCauley, L, Goecker, C, Parker, P, Rudolph, T, Goetz, F, Gerlach, G. (2004) Characterization and
isolation of DNA microsatellite primers in the spiny dogfish (Squalus acanthias). Mol. Ecology
Notes, 4: 494-496.
Nagai, S, McCauley, L, Yasuda, N, Erdner, D, Kulis, D, Matsuyama, Y, Itakura, S, Anderson, D. (2006) Development of microsatellite markers in the toxic dinoflagellate Alexandrium minutum (Dinophyceae). Mol. Ecology Notes. In press
Roberts SB, McCauley, LAR, Devlin RH, Goetz FW. (2004) Transgenic salmon over-expressing growth
hormone exhibit decreased myostatin transcript and protein expression. J. Experimental Biology,
207: 3741-3748.
ABSTRACTS
McCauley, LAR, Erdner, DE, Anderson, D. (2005) Intrapopulation variation of Alexandrium fundyense within the Gulf of Maine: Ribosomal DNA and microsatellite analyses. Third Symposium on Harmful Marine Algae in the U.S, Asilomar, CA, October 2005.
McCauley, LAR , Wehr, JD. (2004) A first evaluation of phylogenetic relationships of freshwater
brown algae using 18S and rbcL sequences. 42nd Northeast Algal Symposium, University of Connecticut, Avery Point, April 2004.
Roy, LA, Trapido-Rosental, H. (1999) Identification of an 18S pseudogene in a symbiotic species of
Symbiodinium. International Conference on Scientific Aspects of Coral Reef Assessment, Monitoring, and Restoration, April 14-16, 1999, Fort Lauderdale, FL: 166.