MariaCurie-SklodowskaUniversity,
Lublin, Poland
Erasmus code: PL LUBLIN01 /
______
STUDY PROGRAMME
1.Course title: Basic and Medical Virology
Course code:
Faculty/Department: Faculty of Biology and Biotechnology, Department of Virology and Immunology
Teacher: AgnieszkaSzuster-Ciesielska, Ph.D., Associate Professor ()
(+48) 81537 59 43
Magdalena Mizerska-Dudka, Ph.D. ()
Lecture/Laboratory, 30 hrs/30 hrs
ECTS credits: 5
Year of study: I (II0) winter semester
Educational and professional goals
As a result of the course the student should be able to:
Explain the origin, structure, replication of viruses, host-virus interactions as well as methods of fighting viral infections..Characterize of viruses families pathogenic for humans. Indicate the way of prophylaxis against viruses. Use the biological models for propagation and isolation of viruses.Work with cell cultures.Apply cell cultures to propagation and titration of viruses. Conducting according the ethical principles and work safety regulations. Awareness of viral diseases risk. Care of the health-oriented personal behavior.
Course description
Lecture contents:General virology: definitions, structure of viruses, classification, replication, virulence, mechanisms of cell injury, acute and persistent infections, viral evolution, host-virus interactions. Characterization of main families of DNA and RNA viruses pathogenic for humans. DNA and RNA tumor viruses. Emerging viruses and zoonosis. Vaccines and antivirals.
Laboratory exercise contents:Organization of BSL-2 laboratory. Principles of working with infectious material. Propagation and isolation of viruses in laboratory animals and chick embryos. Detection and titration of propagated viruses in biological materials. Primary cell culture - isolation and maintenance of chick embryo fibroblasts. Manipulation of cultured cells - primary and continuous cell lines; tissue bank - freezing and thawing cell cultures. Propagation of viruses in cell cultures. Viral cytophatic effect. Virus titration and storage methods.
Literature:
- „Human and Medical Virology” ed. B.W.J. Mahy (2010)
- „Clinical Virology” ed. A.J. Zuckerman (2009)
- "Viruses" D.R.Harper (2012)
Course type: lecture and practical laboratory
Form of assessment: final written exam, continuous assessment of laboratories
Prerequisities: completed biochemistry and microbiology course.
Primary target group: students of biology, biotechnology
2.Course title: Immunology with elements of virology
Course code:
Faculty/Department: Faculty of Biology and Biotechnology, Department of Virology and Immunology
Teacher: AgnieszkaSzuster-Ciesielska, Ph.D., Associate Professor ()
(+48) 81537 59 43
Magdalena Mizerska-Dudka, Ph.D. ()
Lecture/Laboratory:30 hrs/45 hrs
ECTS credits: 6.5
Year of study: III (I0), winter semester
Educational and professional goals
The course provides a basic theoretical and technical study of mechanisms of the immune response, immunodiagnostics, characterization of main families of DNA and RNA viruses pathogenic for humans. Upon completion of the course, the student should be able to:explain mechanisms involved in physiological and pathological immune reactions, define the basic immunological test used in practice, explain the structure, and replication of viruses, characterize of viruses families pathogenic for humans, indicate the way of prophylaxis against viruses.
Course description
Lecture contents: Development of central and peripheral immune system. Innate immune system: function, anatomical barriers, inflammation, complement system, phagocytosis. Adaptive immune system: function, structure and characteristic of antigens, and antibodies, antigen presentation, humoral and cellular immune response. General virology: structure of viruses, replication. Characterization of main families of DNA and RNA viruses pathogenic for humans.
Laboratory exercise contents:Methods of study innate and adaptive immunological response. Central and peripheral immune system, isolation of cells from lymphatic organs. Phagocytosis determination. Proliferation and differentiation of lymphocytes. Macrophage migration inhibition test. Some techniques used in immunodiagnosis (ELISA, Latex tests). Isolation of viruses propagated in laboratory animals and chick embryos. Maintenance of continuous cell lines. Propagation of viruses in cell cultures. Virus titration methods.
Literature:
- "Roitt's Essential Immunology" P.J.Delves, S.J.Martin, D.R.Burton, I.M.Roitt (2011)
- "Human and Medical Virology" ed. B.W.J. Mahy (2010)
- "Viruses" D.R.Harper (2012)
- "Clinical Virology" ed. A.J. Zuckerman (2009)
Course type: lecture and practical laboratory
Form of assessment: final written exam, continuous assessment of laboratories
Prerequisities: completed biochemistry and microbiology course.
Primary target group: students of biology, biotechnology
3.Course title: Virology basic course
Course code:
Faculty/Department: Faculty of Biology and Biotechnology, Department of Virology and Immunology
Teacher: AgnieszkaSzuster-Ciesielska, Ph.D., Associate Professor ()
(+48) 81537 59 43
Magdalena Mizerska-Dudka, Ph.D. ()
Lecture/Laboratory, 15 hrs/30 hrs
ECTS credits: 4
Year of study: I (II0) summer semester
Educational and professional goals
Knowledge: As a result of the course the student should be able to:
- Explain the structure, and replication of viruses, as well as host cells attack methods.
- Characterize of viruses families pathogenic for humans.
- Indicate the way of prophylaxis against viruses.
Skills: As a result of the course the student should be able to:
- Use the biological models for the isolation of viruses.
- Work with cell cultures.
- Apply cell cultures to propagation and titration of viruses.
Attitude: As a result of the course the student will acquire the following attitudes:
- Conducting according the ethical principles and work safety regulations.
- Awareness of viral diseases risk.
- Care of the health-oriented personal behavior.
Course description
Lecture contents:General virology (definitions, structure of viruses, classification, replication). Characterization of main families of DNA and RNA viruses pathogenic for humans.
Laboratory exercise contents:Isolation of viruses propagated in laboratory animals and chick embryos. Maintenance of continuous cell lines; tissue bank - the freezing and thawing the culture. Propagation of viruses in cell cultures. Virus titration methods.
Literature:
- "Human and Medical Virology" ed. B.W.J. Mahy (2010)
- "Clinical Virology" ed. A.J. Zuckerman (2009)
- "Viruses" D.R.Harper (2012)
Course type: lecture and practical laboratory
Form of assessment: final written exam, continuous assessment of laboratories
Prerequisities: completed biochemistry and microbiology course.
Primary target group: students of biology, biotechnology
4.Course title: Biochemistry of Secondary Metabolites
Faculty/Department: Biochemistry Department, Faculty of Biology and Biotechnology
Course code:
Erasmus subject area code:
Number of contact hours: 30 hours – lecture; 30 hours - laboratory
Course duration: I 20, summer semester
ECTS credits: 5
Course description:
The position of secondary metabolism in biochemical processes in living organisms and its regulation at molecular and environmental level. The course covers the major groups of secondary metabolites in microorganisms and plants, from the perspectives of biochemistry and biosynthesis with references to the relationship between biological function of secondary metabolites in defense against different stress. Characteristics of basic secondary metabolites (bacterial, fungal and from plants), their biosynthetic pathways and practical applications for example such as pharmacological compounds.
Literature:
Dewick PM “Medicinal natural products. A biosynthetic approach.” John Wiley & Sons, LTD, 2002
Wink M (ed) “Functions and biotechnology of plant secondary metabolites” Wiley-Blackwell, 2010
Course type: lecture and practical laboratory
Assesment method: written examination, continuous assessment of laboratories
Prerequisities: completed course in biochemistry
Primary target group: students of biology, biotechnology, organic chemistry
Lecturer: dr hab. Anna Jarosz-Wilkolazka
Contact person: Anna Jarosz-Wilkolazka, Email:
Phone: (48 81) 537 50 44
Deadline for application:
Remarks:
Educational and professional goals - student knows the main groups of secondary metabolites produced by microorganisms and plants; can indicate a correlation between structure of secondary metabolites such as alkaloids, terpenoids and antibiotics and their biological activities; is aware of chemical and biological diversities of natural environment.
5.Course title: Microbiology
Faculty/Department: Faculty of Biology and Biotechnology, Department of Genetics and Microbiology
Course code: MIRWM
Teacher: prof. dr hab. Wanda Małek () (48) 81537 59 76 Lecture/Laboratory: 30 hrs / 60 hrs
ECTS credits: 8
Year of study: II (I0), winter semester
Educational and professional goals
The purpose of microbiology course is to familiarize students with the major groups of prokaryotes, their cell structure, function, metabolism, and the role of microorganisms in the environment as well as biotechnology. The studentlearnsthe techniques usedto identify,differentiatemicroorganismsas well asknow thebasic strategiesandmethods to combatpathogenicbacteria.
Course description:
the lectures:
Microbiology beginning. The world of microorganisms. The rules of contemporary classification of microorganisms. Prokaryotic cell structure and functions. Endospores and other resting forms of bacteria. Microbial nutrition: requirements for carbon, nitrogen, iron, phosphorus, sulfur, oxygen, hydrogen. Nutritional types of microorganisms. Microbial growth: measurement of cell number and cell mass, the growth in closed and continuous culture systems. Bacterial biofilms. Metabolism: aerobic and anaerobic respirations, fermentations, chemosynthesis, photosynthesis. Control of microorganism growth by physical and chemical agents. Bacteriophages: structure, lytic and lysogenic cycles. Economic and environmental importance of bacteria.
the classes:
Comparison of different types of bacterial cell wall structure: Gram-negative, Gram-positive, acid-resistant (Gram and Ziehl-Neelsen staining methods). Cytology of bacterial cell: the staining methods of cell wall, genetic material, endospores, capsules. The microbiological techniques: isolation of pure cultures, identification of bacteria on the basis of morphological and physiological features. Measure of the bacterial growth. A types of microbiological culture media: preparation, characteristics. Control of microorganisms:sterilization and disinfection. The effect of environmental factors on bacterial growth: temperature, UV, osmotic pressure, pH, antibiotics. Interactions between organisms: mutualism, antagonism. Identification of microorganisms in dairy products. Bacteriophages: isolation, bacteriophage plaque-count assay, host range determination.
Literature:
“Biology of Microorganisms”- Michael T. Madigan, John M. Martinko, Jack Parker, Prentice Hall International, Inc;
“Microbial Life”- Jerome J. Perry, James T. Staley, Stephan Lory, Sinauer Associates, Publisher Sunderland, Massachusetts
Assesment method: oral or written exam
Prerequisities: basic knowledge of English
Primary target group: biologists, biotechnologists
6.Course title: Immunology
Course code:
Faculty/Department: Faculty of Biology and Biotechnology, Department of Virology and Immunology
Teacher: AgnieszkaSzuster-Ciesielska, Ph.D., Associate Professor ()
(+48) 81537 59 43
Magdalena Mizerska-Dudka, Ph.D. ()
Lecture/Laboratory:30 hrs/45 hrs
ECTS credits: 6.5
Year of study: III (I0), summer semester
Educational and professional goals
The course provides an extensive theoretical and technical study of mechanisms of the immune response in physiological and pathological conditions. Upon completion of the course, the student should be able to:explain mechanisms leading to immune response, name new trends in developing of diagnostic methods and immune therapy, define and perform the basic immunological test used in practice, estimate of immune therapy effectiveness in pathology, orientate for prohealth behavior.
Course description
Lecture contents: Development of central and peripheral immune system. Innate immune system: function, anatomical barriers, inflammation, complement system, phagocytosis. Adaptive immune system: function, structure and characteristic of antigens and antibodies, antigen-antibody reactions, antigen presentation, membrane receptors for antigen, lymphocyte activation, humoral and cellular immune response, the production of effectors, vaccines, immunodeficiency, hypersensivity. Immunological methods and applications.
Laboratory exercise contents:Methods of study innate and adaptive immunological response. Central and peripheral immune system, isolation of cells from lymphatic organs. Phagocytosis determination. Proliferation and differentiation of lymphocytes. Rosette test. Major histocompatibility antigens. Blastic transformation of lymphocytes. Macrophage migration inhibition test. Detection of cells producing antibodies. Some techniques used in immunodiagnosis: agglutination, precypitation, ELISA, Latex tests and some methods used in preparation of monoclonal antibodies.
Literature:
- "Roitt's Essential Immunology" P.J.Delves, S.J.Martin, D.R.Burton, I.M.Roitt (2011)
- "Kuby Immunology" sixth ed. T.J.Kindt, B.A.Osborne, R.A.Goldsby
Course type: lecture and practical laboratory
Form of assessment: final written exam, continuous assessment of laboratories
Prerequisities: completed biochemistry and microbiology course.
Primary target group: students of biology, biotechnology
7.Course title: Ecology
Faculty/Department: Faculty of Biology and Biotechnology, Department of Ecology
Course code: EKOBC
Teacher/Contact person: prof. dr hab. Bożenna Czarnecka (e-mail: ), tel. 48 81537 59 30
Number of hours: Lecture/Laboratory 30 hrs / 45hrs
ECTS credits: 6,5
Course duration: summer semester
Educational and professional goals: The course is designed to allow all the students to broaden their knowledge about interactions among different groups of organisms and their environment.
Course description:
the lectures:
The scope of the content covers the basics of general ecology, including the relationships among organisms and between organisms and the environment occurring at different levels of biological organization; ecological processes; matter exchange and energy balance in the biosphere. Students will also be introduced to current research problems of modern ecology and the nature of its relationship with many areas of mathematical, socio-economic, and natural sciences.
the classes:
The course provides theoretical and practical study of the following issues: types of growth forms among plants and animals, life-histories and adaptive strategies, population ecology, vegetation structure in natural and anthropogenic landscape, ecological processes at the level of plant community (succession, regression, fluctuation, regeneration, degeneration, seasonal dynamics),basic methodology of field ecological observations, designing and conducting simple observations and experiments in laboratory and natural habitats and interpretation of their results, survey of basic habitat characteristic, measurement of soil and water properties, diagnosis of the state of the environment on the basis of indicator species and plant communities, preparation and presentation of field research report.
Literature:
- Begon M., Townsend C.R. Harper J.L. 2006. Ecology. From Individuals to Ecosystems. Blackwell Publishing, Malden, USA.
- Crawley M.J. 1997. Plant Ecology. Blackwell Science, Oxford, UK.
Course type: lecture, laboratory with outdoor practice
Form of assessment Lecture: the written test exam composed of different forms of questions (true/false, multiple choice, short answer); Laboratory: written test and field research report
Prerequisities: Basic knowledge of English, basic knowledge of botany and zoology
Primary target group: Biology, Biotechnology and Environmental Science Students
8. Course title: Plant Embryology
Faculty/Department: Faculty of Biology and Biotechnology, Department of Plant Anatomy and Cytology
Course code: EMRJB
Teacher/Contact person: KrystynaWiniarczyk ;
Number of contact hours: Lecture/Laboratory 30 hrs /45 hrs
ECTS credits: 6
Course duration: I (IIº) year, winter semester
Educational and professional goals:
- To gain knowledge in the field of embryology, especially in structures and functions of organs involved in sexual reproduction of flowering plants.
- To develop skills in preparation of microscopic slides and interpretation of tissue sections
Course description:
the lectures:
Heterogenic cycle and sex determination in plants. Bryophytes: sporangium and sporogenesis; archegonia, antheridia and gametes. Generative organs of ferns and horsetails. Heterospory in club mosses and water ferns. Gymnosperms: pollen sac development, microsporogenesis, pollen grain development, ovule development, macrosporogenesis, perisperm, archegonia, embryo. Angiosperms: anther development, microsporogenesis, pollen development, types of: ovules, macrosporogenesis and embryo sacs; pollination, progamic phase, fertilization, embryo, seed development, apomixes.
the classes:
- Practical preparation of microscopic slides
- Methods of tissue section staining
- Use of different types of microscopic techniques
Literature:
1Lersten N.R. Flowering Plant Embryology. 2004
2 BatyginaT.B. Embryology of Flowering Plants vol 1,2,3. 2009
Course type: Lecture, Laboratory
Form of assessment: Test (written examination), continuous assessment of laboratoryPrerequisities: Completed course in: general and taxonomic botany; plant anatomy
Primary target group: student’s of general biology course
9.Course title: Animal histology and embryology
Faculty/Department: Faculty of Biology and Biotechnology, Department of Comparative Anatomy and Anthropology
Course code: EHZAG
Teacher/Contact person:
MonikaHułas-Stasiak, 81 5397 59 08
Joanna Jakubowicz-Gil, , 81 5397 59 08
Number of contact hours: Laboratory 90 hrs
ECTS credits: 6.5
Course duration: III year (I°), summer semester
Educational and professional goals
- To acquire an in –depth knowledge of human body structure at the microscopic level
- To know the relationship between the histological structure and function of differentiated/specialized cell types, tissue types and organs of human
- To develop an understanding how organ integrity and functions are maintained by the organization of cells and tissues
- To identify cells and tissues and describe their function
- To observe and study the microscopic anatomy of selected differentiated/specialized cell types, tissue types and organs of human
- To develop a professional histological terminology
- To know some of the techniques that are used to investigate histology
- To gain experience in reading and evaluating scientific literature
- To become familiar with some of the clinical applications of histology in health and disease.
- To introduce students to developmental anatomy of the human and animal embryo.
- To emphasis anatomical change with some discussion of developmental mechanism and physiology.
Course description:
the classes:
Histology is an introduction to the microscopic structure of cells, tissues and organs. The emphasis of the course is on the study of human body. This course provides the students with the opportunity to use the light microscope to study stained and mounted sections of mammalian tissues. The aim of this course is to allow the students to gain an understanding of the human body on a microscopic level and to develop an appreciation of intricate relationship among various organ systems. The focus of embryology is on the anatomy of vertebrate embryogenesis with specific emphasis on humans. Topics include fertilization, implantation, gastrulation, neurulation and organogenesis of a variety of structures.
Literature:
1 Sadler TW. Medical Embryology. Lippincott Williams and Wilkins, 2006
2 Alan Stevens, James Lowe. Human Histology, 2010
Course type: lecture and practical laboratory
Form of assessment written exam
Prerequisities: basic knowledge of English
Primary target group: biologists, biotechnologists