LAB: Virtual Fruit Fly Genetics – ANALYSIS SHEET

PARENT ONE-GENE CROSS: Record the appropriate information in the data table provided.

Cross / Parent (sex/ phenotype) / Sex of Offspring / Phenotype of Offspring / Number with Phenotype
EXAMPLE:
Parent cross
(P cross  F1) / Female (wild) x Male (vestigial) / Female / wild-type (+)
Male / wild-type (+)
MONOHYBRID CROSS
(F1  F2) / (use the offspring from the F1 generation to produce the F2!) / Female / wild-type(+)
Male / wild-type(+)
Female / vestigial
Male / vestigial

CHECKPOINT / “Check for Understanding”:

1) What does an incubator do?

2) What is used to anesthetize the flies?

3) Why would a scientist want to do this to the flies?

4) What does the notation “+” mean?

5) What does the notation “VG” mean?

6) What is meant by “monohybrid” cross?

7) For this first cross, what was the “wild-type” phenotype?

8) For this first cross, what was the “vestigial” phenotype?

9) Identify the dominant trait and the recessive trait. Explain how you figured this out.

> DOMINANT TRAIT:

> RECESSIVE TRAIT:

> How do you know?

10) Identify the genotype and phenotypes of the parents for the next cross. (Symbols: “+” for wild-type; “VG” for vestigial.)

> Parent genotypes for next cross:x

> Parent phenotypes for next cross:x

11) Predict the outcome of the next cross and the ratio you expect to find in the F2 generation.

PREDICTION:

Do the cross to get the F2 generation! Record results in data table.

12) How many wild-type offspring resulted from the F1 cross? ______How many vestigial?______

13) What is the ratio of wild-type:vestigial flies (to the tenths place)? ______

TWO-GENE CROSS: Record the appropriate information in the data table provided.

TWO-GENE Cross / Parent (sex/ phenotype) / Sex of Offspring / Wing Phenotype of Offspring / Eye Color Phenotype of Offspring / Number with Phenotype
PARENT CROSS
DIHYBRID CROSS
(F1  F2)

CHECKPOINT / “Check for Understanding”:

1) What is a TWO-GENE CROSS?

2) What does the notation “SE” mean?

3) What does the notation SE:VG mean (read as “SE & VG”)?

4) Identify the dominant traits and the recessive traits. Explain how you figured this out.

> DOMINANT TRAITS:

> RECESSIVE TRAITS:

> How do you know?

5) Identify the genotype and phenotypes of the parents for the next cross.

> Parent genotypes for next cross:x

> Parent phenotypes for next cross:x

6) Predict the outcome of the next cross and the ratio you expect to find in the F2 generation.

PREDICTION:

Do the cross to get the F2 generation! Record results in the data table above.

7a) Write the total number of flies that are wild-type for both wing shape & eye color: ______
(example genotype: +VG+SE or ++ ++)

7b) Write the total number of flies that are wild-type for wing shape & sepia for eye color: ______

7c) Write the total number of flies that have vestigial wing shape & are wild-type for eye color: ______

7d) Write the total number of flies that have vestigial wing shape & have sepia for eye color: ______

7e) What is the ratio for the traits above (to the tenths place)? ______:______:______:______
(HINT: use the complete recessive for “1”)

8) What is the genotype for a fly in 7c? ______or ______