Title:
Use of Gas Cylinder / Rev No: / 2
Issue Date: / 25-Nov-2016
Page: / 1 of 2

Prepared by Approved by Approved Date Revision Date

Wang MingDr. Chen Chia-Hung 1.12.2016 1.12.2019

1.0OBJECTIVE

This is a procedure for safely transporting gas cylinders and for safely using gas cylinders in the Integrated Microfluidicslaboratory at the National University of Singapore (NUS) Department of Biomedical Engineering and SINAPSE .

2.0SCOPE

This SOP is applicable to all lab users.

3.0 PROCEDURES

3.1Before operation

  1. Check if tank is secured to the wall or lab bench.
  2. Check the main stem valve on the gas cylinder.
  3. Check connections for leakage using a soap solution or a leak detector.

3.2 During use

  1. Open Main valves to a point where gas flows into the system at the necessary pressure but not completely open. This will allow for a quicker shut off in the event of an emergency.
  2. If there seems to be a leak, check connections for leaks using a soap solution or a leak detector.

3.3After use

  1. Close the main stem valve on the gas cylinder.
  2. Then remove all pressure from the regulator gauges by opening the second stage valve.
  3. Main valves should be opened to a point where gas flows into the system at the necessary pressure but not completely open. This will allow for a quicker shut off in the event of an emergency.

4.0 PRECAUTIONS!

  1. Do not remove any labels or other form of identification from any gas cylinder.
  2. Use a tank cart to move the gas cylinder from the loading dock to the laboratory.
  3. When the tank is empty, put the cap back on the cylinder, mark the tag as empty, and return the gas cylinder to the loading dock using the tank cart.
  4. Do not use Teflon tape with metal-to-metal fittings as it spreads the threads and can cause leaks.