Next Gen DNA Sequencing Services Inquiry / Sample Submission form for existing / repeat customers.(Please fill out all fields where applicable, if you are unsure of an answer just put “do not know” and we will assist you in determining the answer.)

Upon completion please send as an attachment to

User Information:

Your Name (Last, First): Last, First

BPF User Account ID*bpfuserid

Email:

Type of Sample Prep / Analysis: (Please complete a separate sheet for each type)

Genomic Re-Sequencing Small RNA (microRNA) Analysis

ChIP-Sequencing Custom Tag Counting

Mate Pair Resequencing Other

Sample Information:

1. Organism:Rat

2. Number of Samples: 4

3. I have access to a reference genome or transcript database.Yes

  1. I plan to perform my own sample prep: Yes (skip to question 10) No

Not sure, would like to discuss.

Sample Prep Information:

If you would like the BPF to perform sample prep or you are not sure please complete questions 5 to 9.

5. Amount of material available per sample (μg):20 ng

If applicable:

6. Size distribution of the IP material: 0-500 bp

7. Size range of the small/micro RNAs for your organism:

8. Types of mutations you are looking for:

9. Please provide any other information you feel would be helpful to us or a brief description of your customized project with as much detail as possible to help us determine feasibility.

If you are doing your own sample prep please complete question10.

10. What adaptors did you use for your constructs?

AB StandardAdaptors

AB Mate Pair Adaptors

Custom Adaptors (please provide detailed information below (primer sequences, etc.)

QC Information:

11. What QC measures did you use to determine the concentration of functional library material?

For any method used, please provide documentation with sample so that we may review it and attach the records to your sample in our LIMS.

Note: It is absolutely critical for the concentration of properly constructed library molecules to be determined as accurately as possible in order to achieve optimal cluster density. QPCR is the recommended method as spectrophotometer readings can be off by >50% in terms of functional material. A combination of these methods (QPCR, spectrophotometer and Bioanalyzer are recommended to determine the optimal concentration to use. Ideally, 1.5 to 2.5 pMols of functional material is recommended in a volume of 5uls for each lane. THIS IS A CRITICAL STEP!!!

QPCR assay (please provide curves and conc. Information).

Spectrophotometer (Nanodrop, etc.)

Agilent Bioanalyzer

Other method (please describe below)

I have NOTQC’d my sample or determined functional concentration for cluster and sequencing. I would like the facility to do this for me.

Data Analysis Information:

At this time we are offering a limited amount of data analysis assistance. We will discuss your data analysis needs in further detail at the time of consultation, if necessary.

Data Distribution:

Please continue to use my existing data distribution channels / method.

Please change my data distribution method as requested below.

Harvard Labs

I have an ecommons account and would like to use the BPF's storage on the HMS Storage Array for a small monthly fee.

I have storage available and would like to retrieve my files via ftp.

I will provide a portable hard drive to obtain my files.

I would like to lease a portable hard drive from the BPF to obtain my files.

I am unsure and would like to discuss this further.

Non-Harvard Labs

I have storage available and would like to retrieve my files via ftp.

I will provide a portable hard drive to obtain my files.

I would like to lease a portable hard drive from the BPF to obtain my files.

I am unsure and would like to discuss this further.

Please email this completed word document as an attachment to . We will send an acknowledgement as soon as we receive your document. You will be contacted to set up further consultations upon our review of your project. During the consultation you will recieve final project specifications and a formal quote for all services. A purchase order must be submitted to secure a position in the Genome Analyzer queue.