Kathryn J. Coyne
Kathryn J. Coyne
University of Delaware Phone (office): (302) 645-4236
College of Earth, Ocean and Environment Phone (lab): (302) 645-4288
School of Marine Science and Policy FAX: (302) 645-4007
700 Pilottown Rd. e-mail:
Lewes, DE 19958
Education
Ph.D. Biochemistry, 1997, University of Delaware, Newark, DE
B.S. Chemistry, 1986, University of Delaware, Newark, DE
A.A.S. Biology, 1979, SUNY Morrisville Ag. and Tech. College, Morrisville, NY
Professional Experience
2012-present Associate Professor, Marine Biosciences, College of Earth, Ocean and Environment, University of Delaware, Lewes, DE
2005-2012 Assistant Professor, Marine Biosciences, College of Earth, Ocean and Environment, University of Delaware, Lewes, DE
2004-2005 Research Scientist, Graduate College of Marine Studies, University of Delaware, Lewes, DE
1997-2004 Postdoctoral Research Assistant, Graduate College of Marine Studies, University of Delaware, Lewes, DE
1991-1997 Graduate Research Assistant, Department of Chemistry and Biochemistry, University of Delaware, Newark, DE
1986-1993 Chemistry Instructor, Wesley College, Dover, DE
1986-1988 Chemist, L.D. Caulk Co., Milford, DE
Professional Activities
NOAA PCM HAB (Prevention, Mitigation and Control of Harmful Algal Blooms) review panel, Dec., 2011.
HAB Task Force, Meeting, Baltimore, MD, Dec. 7, 2011, Invited speaker: Coyne, K.J., Warner, M. E., Pokrzywinski, K., Tilney, C. Characterization of an algicidal agent produced by Shewanella sp. IRI-160 and its impact on dinoflagellate physiology and microbial community dynamics.
Steering Committee, Sixth Symposium on Harmful Algae in the US. To be held in Austin, TX, November 13 - 17, 2011.
Editorial Board, Frontiers of Microbial Chemistry, April, 2011 - present.
NOAA ECOHAB (Ecology and Oceanography of Harmful Algal Blooms) review panel Feb., 2010.
RNA Applications, a 2-week workshop at the Institute for Microbial Biotechnology and Metagenomics (IMBM), May 12 - 23, 2008, University of the Western Cape, Cape Town, South Africa. This workshop was organized and conducted at the request of researchers at the IMBM.
Harmful Algal Blooms in the Mid-Atlantic Forum, Apr. 28, 2008, Virden Center, University of Delaware, Lewes, DE. Invited speaker.
Co-Host, Atlantic Estuarine Research Society Spring Meeting, March 13-15, 2008, Virden Center, University of Delaware, Lewes, DE.
EPA ECOHAB (Ecology and Oceanography of Harmful Algal Blooms) review panel, Jan., 2008.
Aureococcus Genome Consortium: Annotating the genome for Aureococcus anophagefferens. Aureococcus Genome Jamboree, Oct. 3-5, 2007, Southampton, NY, invited participant.
QPCR Enumeration of HAB Species in Environmental Water Samples, a 4-hour workshop conducted during the 12th International Conference on Harmful Algae, Sept. 6, 2006, Biological Institute, Copenhagen, Denmark. This workshop was organized and conducted as part of my NOAA-MERHAB –funded project.
QPCR Enumeration of HAB Species in Environmental Water Samples, a full-day, hands-on workshop for DNREC personnel, April 6, 2006, University of Delaware, Lewes, DE. This workshop was organized and conducted as part of my NOAA-MERHAB –funded project.
Gordon Research Conference on Mycotoxins and Phycotoxins: Application of Emerging Technologies to Mycotoxin and Phycotoxin Research, June 19-24, 2005. Invited speaker.
NOAA MERHAB (Monitoring and event response to harmful algal blooms) review panel, May, 2005.
Development and Application of Ecogenomics for Water Quality Assessment, EPA EMAP 2002 Symposium, Kansas City, MO, May 9-10, 2002. Invited speaker.
Guest Lecturer, 1998, 2000, and 2003, Methods in Molecular Biology, MAST616, Graduate College of Marine Studies, University of Delaware, Lewes, DE.
Workshop: Molecular Approaches for Identification and Environmental Detection of Pfiesteria piscicida and Pfiesteria-like Dinoflagellates, Center of Marine Biotechnology, Baltimore, Maryland, September 3-4, 1999. Invited participant.
Technical Advisor, 1998 – 2002, Environmental Science Dept., Sussex Technical High School, Georgetown, DE.
Reviewer: Aquatic Microbial Ecology, Hydrobiologia, Limnology and Oceanography: Methods, Harmful Algae, Marine Biotechnology, Protist, Aquatic Ecology, Plant and Soil Science.
Oceanographic Cruises
Extreme 1, May 7-June 5, 1999, 9º N, East Pacific Rise Vent Expedition
Extreme 2001, Oct. 13-Nov. 1, 2001, 9º N, East Pacific Rise Vent Expedition
Extreme 2007, Oct. 18-Nov. 8, 2007, Guaymas and 9º N, EPR
Extreme 2008, Nov. 9-Dec. 3, 2008, Guaymas and 9º N, EPR
Thesis Committee Member
Clinton E. Hare, 2003, M.S., UD, College of Earth, Ocean and Environment
Sara Handy, 2007 Ph.D., UD, College of Earth, Ocean and Environment
Elif Demir, 2007 Ph.D., UD, College of Earth, Ocean and Environment
Karen Pelletreau, 2008 Ph.D., UD, College of Earth, Ocean and Environment
Kevin Portune, 2008 Ph.D., UD, College of Earth, Ocean and Environment
Alicia Mangum, 2009, M.S., Delaware State University
Vanessa Michelou, 2009 Ph.D., UD, College of Earth, Ocean and Environment
Lauren Salvitti, 2010, M.S, UD, College of Earth, Ocean and Environment
Jennifer Stewart, 2011, Ph.D. UD, College of Earth, Ocean and Environment
MichaelMcGinley, 2012, Ph.D., UD, College of Earth, Ocean and Environment
Katherine Lee, 2012, M.S., UD, College of Earth, Ocean and Environment
Kaytee Pokryzwinski, M.S. student, UD, College of Earth, Ocean and Environment
Christopher Main, Ph.D. student, UD, College of Earth, Ocean and Environment
Colleen Bianco, M.S. student, UD, College of Earth, Ocean and Environment
Bingran Cheng, M.S. student, UD, College of Earth, Ocean and Environment
Charles Tilney, Ph.D. student, UD, College of Earth, Ocean and Environment
Current Funding
Coyne, K. J. and Stewart, J. J. DE Sea Grant: Development of microalgae for bioremediation of flue gas coupled to production of renewable energy. DE Sea Grant, Feb. 1, 2012 - Jan. 31, 2014. $186,064.
Coyne, K. J. and Warner, M. PCM HAB: Characterization of an algicidal agent produced by Shewanella sp. IRI-160 and its impact on dinoflagellate physiology and microbial community dynamics. NOAA-CSCOR, Sept., 2010 - Aug. 2014. $536,895.
Greenfield, D. and Coyne, K.J. MERHAB 2009: Comparative analysis of quantitative detection methods for enumeration of HAB species: Applications for resource management. NOAA-CSCOR, Sept., 2010 - Aug. 2014. $337,535.
Previous Funding
Coyne, K. J. and Boyd, F. EPSCoR: The Vibrio-HAB connection: Evaluating the interactive effects of iron availability and Vibrio spp. on toxicity of harmful algal species. DE-EPSCoR, Feb. 1, 2012 - Jan. 31, 2013. $45,797.
Coyne, K.J. and M. Warner. The interactive effects of light and nutrient source on the molecular regulation of nitrogen assimilation, photochemistry, and growth of two raphidophyte species from Delaware’s Inland Bays. NSF, April 15, 2008 – Apr. 14, 2012. $450,000.
Coyne, K.J. Development of diatoms as indicators of water quality in Delaware's inland bays. DE Sea Grant, Feb. 2009 – Jan. 2012. $188,822
Coyne, K. J. The HAB-Vibrio Connection: Investigating interactions between harmful algal bloom species and pathogenic Vibrio spp. in Delaware’s inland bays. DE Sea Grant. Feb. 2011- Jan. 2012. $64,231.
Ozbay, G. and K.J. Coyne. Benthic diatom assemblages as environmental indicators in Blackbird Watershed, Delaware. NOAA-ECSC, Aug. 1, 2008 - July 31, 2011. $148,770.
Hutchins, D.A., K.J. Coyne, and M. Warner. The future of harmful algal blooms: an empirical approach to predicting the combined impacts of rising CO2, temperature, and eutrophication. EPA-ECOHAB, Mar. 15, 2007 – Mar. 14, 2011. $549,993.
Coyne, K.J. Benthic diatoms as indicators of water quality in Delaware’s Inland Bays. Center for the Inland Bays, Oct. 1, 2008 – Sept. 30, 2010. $19,935.
Gobler, C.J. and K.J. Coyne. The impact of nutrients, zooplankton, and temperature on growth of, and toxin production by, cyanobacteria blooms in the upper reaches of the Chesapeake Bay. EPA-ECOHAB, Mar. 15, 2007 – Mar. 14, 2010. $16,000 (UD subcontract).
Farrell, J., K. J. Coyne and E. Whereat. Recreational water bacteria sampling and HAB monitoring for Delaware coast and bay sites. EPA/DNREC. $15,000.
Ozbay, G. and K. J. Coyne. Identifying physical and chemical parameters affecting the distribution of pathogenic Vibrio species in Delaware’s Inland Bays: Application of molecular methods for population assessment. DE-EPSCoR, Feb. 1, 2009 – Nov. 30, 2009. $30,000.
Coyne, K.J. and G. Ozbay. Nitrate Reductase Activity in Mixed Harmful Algal Populations from Delaware’s Inland Bays. NSF Delaware-EPSCoR, Oct. 1, 2006 – Sept. 30, 2007. $49,767.
Cary, SC and K.J. Coyne. A Culture-Independent Approach to Develop a Sensitive Genetic-
Based Detection and Enumeration Capability for Didymosphenia geminata. MAF Biosecurity New Zealand, Jan., 2006 – July, 2007. NZ$331,206.
Hutchins, D.A. and K.J. Coyne. A new HAB invader in the Inland Bays: Initial isolation and characterization of a low-salinity raphidophyte species unknown to science. Center for the Inland Bays, Oct. 1, 2005 – Sept. 30, 2006. $15,000.
Cary, S.C. and K.J. Coyne. MERHAB 2004: Quantitative molecular detection of multiple HAB species. NOAA – MERHAB, Sept. 1, 2004 – Aug. 31, 2007. $299,239.
Hutchins, D.A., K.J. Coyne, M.A. Doblin and S.C. Cary. Investigation of toxic Raphidophyte population dynamics using molecular and physiological tools. EPA - ECOHAB, October 1, 2003 – September 30, 2006. $567,331.
Cary, S.C. and K.J. Coyne. Molecular assessment of a toxic harmful algal bloom species, Anabaena planktonica: An international collaboration. Center for International Studies, University of Delaware, June, 2003 – June, 2004. $9,900.
Memberships
Phycological Society of America
International Society for the Study of Harmful Algae
American Society of Limnology and Oceanography
Patents
IRI-160AA: A dinoflagellate-specific algicidal compound produced by Shewanella strain IRI- 160. Patent pending.
Stewart, J. J. and Coyne, K. J. Novel Nitrate Reductase Fusion Proteins and Uses Thereof. U.S. Patent No. 8,409,827 B2. Issued April 2, 2013.
Publications
Coyne, K. J., Countway, P., Pilditch, C., Caron, D., Lee, C. and Cary, S. C. (2013) Diversity and distribution of ciliates associated with microbial mats at Guaymas hydrothermal vent site. In Press, J Euk Microbiol.
Hennige, S. Coyne, K. J., MacIntyre, H., Liefer, J. and Warner, M. E. (2013) The photobiology of Heterosigma akashiwo: Photoacclimation, diurnal periodicity and its ability to rapidly exploit exposure to high light. Journal of Phycology 49 (2):349-360. DOI: 10.1111/jpy.12043.
Demir-Hilton, E., Hutchins, D. A., Czymmek, K. J. and Coyne, K. J. (2012) Description of Viridilobus marinus (gen. et sp. nov.), a new raphidophyte from Delaware's inland bays. Journal of Phycology 48:1220-1231.
Pokrzywinski, K. L., Place, A. R., Warner, M. E. and Coyne, K. J. (2012) Investigation of the algicidal exudate produced by Shewanella sp. IRI-160 and its effect on dinoflagellates. Harmful Algae 19:23-29.
Stewart, J. J. and Coyne, K. J. (2011) Analysis of raphidophyte assimilatory nitrate reductase reveals unique domain architecture incorporating a truncated hemoglobin. Plant Molecular Biology 77 (6):565-575.
Gobler, C. J., Berry, D. L., Dyhrman, S. T., Wilhelm, S. W., Salamov, A., Lobanov, A. V., Zhang, Y., Collier, J. L., Wurch, L. L., Krustka, A. B., Dill, B. D., Shah, M., VerBerkmoes, N. C., Kuo, A., Terry, A., Pangilinan, J., Lindquist, E., Lucas, S., Paulsen, I., Hattenrath-Lehmann, T., Talmage, S., Walker, E., Koch, F., Burson, A., Marcoval, M., Tang, Y., LeCleir, G., Coyne, K., Berg, G., Bertrand, E., Saito, M., Gladyshev, V. and Grigoriev, I. (2011) Niche strategy of harmful algae revealed through comparative genomics of competing phytoplankton species. PNAS, 108 (11):4352-4357.
Fredrickson, K.A., Strom, S. L., Crim, R. and Coyne, K. J. (2011) Inter-strain variability in physiology and genetics of Heterosigma akashiwo (Rahpidophyceae) from the west coast of North America. Journal of Phycology 47:25-35.
Coyne, K. J. (2010) Nitrate reductase (NR1) sequence and expression in the harmful alga Heterosigma akashiwo (Raphidophyceae). Journal of Phycology 46: 135-142.
Portune, K. J., Coyne, K. J., Hutchins, D. A., Handy, S. M. and Cary, S. C. (2009) Germination of Heterosigma akashiwo and Chattonella subsalsa (Raphidophyceae) cysts in Delaware’s Inland Bays, DE. Aquatic Microbial Ecology 55: 229-239.
Demir, E., Coyne, K. J., Doblin, M. E., Handy, S. M. and Hutchins, D. A. (2008) Microzooplankton grazing rates on Heterosigma akashiwo in the Delaware Inland Bays: A species-specific approach combining quantitative real-time PCR (QPCR) and dilution methods. Microbial Ecol. 55: 583-594.
Handy, S. M., Demir, E., Hutchins, D. A., Portune, K. J., Whereat, E. B., Hare, C. E., Rose, J. M., Warner, M., Farestad, M., Cary, S. C., and Coyne, K. J.(2008) Using quantitative real-time PCR to study competition and community dynamics among Delaware Inland Bays harmful algae in field and laboratory studies. Harmful Algae 7:599-613.
Doblin, M. E., Coyne, K. J., Rinta-Kanto, J. M., Wilhelm, S. W., and Dobbs, F. C. (2007) Dynamics and short-term survival of cyanobacteria species in ballast water—implications for HAB invasions. Harmful Algae 6:519-530.
Gobler, C. J., Davis, T. W., Coyne, K. J., and Boyer, G. L. (2007) The interactive influences of nutrient loading and zooplankton grazing on the growth and toxicity of cyanobacteria blooms in a eutrophic lake. Harmful Algae 6:119-133.
Coyne, K. J., Hare, C. E., Popels, L. C., Hutchins, D. A., and Cary, S. C. (2006) Distribution of Pfiesteria piscicida cyst populations in sediments of the Delaware Inland Bays, USA. Harmful Algae 5:363-373.
Handy, S. M., Cary, S. C., Hutchins, D. A., and Coyne, K. J. (2006) Simultaneous enumeration of multiple raphidophyte species by quantitative real-time PCR: capabilities and limitations. Limnol. Oceanog. Methods 3:193-204.
Zhang, Y., F.-X. Fu, E., Whereat, K.J. Coyne and D. A. Hutchins. (2006) Bottom-up controls on a mixed-species HAB assemblage: A comparison of sympatric Chattonella subsalsa and Heterosigma akashiwo (Raphidophyceae) isolates from the Delaware Inland Bays, USA. Harmful Algae 5:310-320.
Phleger, C. F., M. M. Nelson, A. K. Groce, S. C. Cary, K. J. Coyne, J. A. E. Gibson, and P. D. Nichols. (2005) Lipid biomarkers of deep-sea hydrothermal vent polychaetes - Alvinella pompejana, A. caudata, Paralvinella grasslei and Hesiolyra bergii. Deep Sea Research Part I: Oceanographic Research Papers 52:2333-2352.
Coyne, K. J., Handy, S. M., Demir, E., Whereat, E. B., Hutchins, D. A., Portune, K. J., Doblin, M. E. and Cary, S. C. (2005) Improved quantitative real-time PCR assays for enumeration of harmful algal species in field samples using an exogenous DNA reference standard. Limnology and Oceanography Methods 3:381-391.
Handy, S. M., Coyne, K. J., Portune, K. J., Demir, E., Doblin, M. E., Hare, C. E., Cary S. C., and Hutchins, D. A. (2005) Evaluating vertical migration behavior of harmful raphidophytes in the Delaware Inland Bays utilizing quantitative real-time PCR. Aquatic Microbial Ecology 40:121-132.
Phleger, C. F., M. M. Nelson, A. K. Groce, S. C. Cary, K. J. Coyne, and P. D. Nichols. (2005) Lipid composition of deep sea hydrothermal vent tubeworm Riftia pachyptila, crabs Munidopsis subsquamosa and Bythograea thermydron, mussels and limpets. Comp. Biochem. Physiol., 141:196-210
Coyne, K. J. and S. C. Cary (2005) Molecular approaches to the investigation of viable dinoflagellate cysts in natural sediments from estuarine environments. Journal of Eukaryotic Microbiology 52:90-94.