UCSF Lab Standard Operating Procedure
Chemical Process, Name or Hazard Class:
5-Iodo2’-deoxy-D-uridine
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Type of SOP: ☐ Process ☒Hazardous Chemical ☐ Hazardous Class
Purpose
5-Iodo2’-deoxy-D-uridine is a reproductive toxin. It is an antitumor nucleoside enantiomer used as an antiviral agent.
Physical & Chemical Properties/Definition of Chemical Group
CAS#: 54-42-2
Class: Reproductive toxin
Molecular Formula: C9H11IN2O5
Form (physical state): solid needles when crystallized form ethanol
Color: white
Boiling point: N/A
Synonyms: idoxuridine; 2' -deoxy-5-iodouridine; 1-(2-deoxy-beta-D-ribofuranosyl)-5-iodouracil; 1-beta-D-2' -deoxyribofuranosyl-5-iodouracil; 5-iododeoxyuridine; 5-iodo-2' –deoxyuridine; 5-iodouracil deoxyriboside; Allergan 211; Denrid; Emanil; Herpesil; Herpidu; Herplex; Idexur; Idoxene; Idoxuridin; IDU; Iducher; Idulea; Iduoculos; Idur; Idurin; IUDR; 5IUDR; Joddeoxiuridin; Kereci; NSC-39661; Opthalmadine; SK&F 14287 Stoxil; Synmiol; Virunguent; Vistaspectran; Zostrum;
Potential Hazards/Toxicity
Wheeze and cough, shortness of breath, burning in the mouth, throat, or chest. Reproductive disorders and genetic alterations. IdU is incorporated into tissue DNA in place of thymidine in animals. IdU-substituted DNA replaces normal DNA and chromosomal proteins are altered through: chromosome lengthening, chromatid breakage, and changed sister chromatid exchange frequencies. Meiosis and mitosis are affected. IdU is suspected to be a carcinogen resulting in heritable genetic damage. It is harmful to the unborn child. IdU is not mutagenic—Ames test; but IdU is mutagenic—micronucleus and sperm abnormality assay. IdU is a strong teratogen in rodents. IdU integrates into DNA and affects the colon, stomach, bone marrow, and spleen.
Engineering Controls
Always handle IdU inside a certified chemical fume hood or a ducted biosafety cabinet.
Personal Protective Equipment (PPE)
Respirator Protection
If lab personnel would like to use respirator on a voluntary basis, they must be trained and fit-tested by EH&S. This is a regulatory requirement. (http://or.ucsf.edu/ehs/8193-DSY/version/default/part/4/data/)
Hand Protection
Double nitrile gloves are required.
NOTE: Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with 5-Iodo-2’-Deoxy-D-Uridine
Refer to glove selection chart from the links below:
http://www.ansellpro.com/download/Ansell_8thEditionChemicalResistanceGuide.pdf
OR
http://www.allsafetyproducts.biz/page/74172
OR
http://www.showabestglove.com/site/default.aspx
OR
http://www.mapaglove.com/
Eye Protection
ANSI Approved safety glasses and/or a face shield are recommended.
Skin and Body Protection
Lab coats should be worn. These laboratory coats must be appropriately sized for the individual and be buttoned to their full length. Laboratory coat sleeves must be of a sufficient length to prevent skin exposure while wearing gloves. Full length pants and close-toed shoes must be worn at all times by all individuals that are occupying the laboratory area. The area of skin between the shoe and ankle should not be exposed.
Hand Protection
Nitrile gloves are recommended.
Refer to glove selection chart from the links below:
http://www.ansellpro.com/download/Ansell_8thEditionChemicalResistanceGuide.pdf
OR
http://www.allsafetyproducts.biz/page/74172
OR
http://www.showabestglove.com/site/default.aspx
OR
http://www.mapaglove.com/
Hygiene Measures
Wash thoroughly after handling. Wash hands before eating. Remove contaminated clothing and wash before reuse.
First Aid Procedures
If inhaled
Remove rapidly to clean air. Administer rescue breathing if necessary and call emergency services. Seek medical attention if needed.
In case of skin contact
Remove contaminated clothing and wash skin with soap and water; avoid rubbing and increases in temperature. Seek medical attention promptly.
In case of eye contact
Rinse immediately with copious amounts of running water for at least 15 minutes. Seek attention of a licensed Ophthalmologist promptly.
If swallowed
Drink lots of water/milk. Induce vomiting. Seek medical attention (refer gastric lavage).
Spill and Accident Procedure
Chemical Spill Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-206-8522 (SFGH only)
Spill – Assess the extent of danger. Assist contaminated or injured persons. Evacuate the spill area. Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or absorbent material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).
Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear plastic bags, label and take to the next chemical waste pick-up.
Large (>1 L) – Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-206-8522 (SFGH only) for assistance.
Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes. If discomfort persists, proceed to the Emergency Department. If no further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800-222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-476-1300 during work hours, or 9-911 during non-working hours and weekends.
Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water for 15 minutes by forcibly holding the eye open. If discomfort persists, proceed to the Emergency Department. If no further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800-222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-476-1300 during work hours, or 9-911 during non-working hours and weekends.
Medical Emergency Dial 9-911 (campus phone) or 476-6911 (cell phone)
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Non-Life Threatening Emergency– Go to Occupational Health Programs (OHP) Clinic, 415-885-7580, 2330 Post Street, Suite 460 Hours of Operation for Appointments: Monday - Friday 7:30 a.m. - 4:00 p.m. (except Holidays).
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick nurse by dialing 415-353-7842 (STIC).
Decontamination/Waste Disposal Procedure
Clean contaminated surfaces with soap and water and paper towels. Dispose of the paper towels as hazardous waste.
Safety Data Sheet (SDS) Location
Online SDS can be accessed at http://or.ucsf.edu/ehs/7241-DSY/msds.html
Protocol/Procedure
Quantities covered by this SOP:
______(g , ml) to ______(g, ml)
Temperature range covered by this SOP:
__ °C – __ °C
General Overview and Purpose:
Enter the experimental purpose
Procedure:
Enter experimental procedure. You can copy procedure from your lab notebook or from literature.
NOTE
Any deviation from this SOP requires approval from the Principal Investigator.
5-Iodo2’-deoxy-D-uridine 5 Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)