Tricaine Methanesulfonate (MS-222)

Standard Operating Procedure

Revision Date: 05/02/2018

Applies To: Employees responsible for writing lab-specific standard operating procedures; and all faculty, staff, students, and other employees working with tricaine methanesulfonate.

Laboratory Director (LD) Approval is Required Prior to Performing this Procedure

This standard operating procedure (SOP) outlines the handling and use of Tricaine Methanesulfonate (MS-222). Review this document and supply the information required in order to make it specific to your facility. In accordance with this document, laboratories should use appropriate controls, protective equipment, and disposal techniques when handling Tricaine Methanesulfonate (MS-222).

Description

Tricaine Methanesulfonate (MS-222) is commonly used as an anesthetic for fish and amphibians. It is most commonly administered by adding it directly to the water in which the species is held and is already acclimated. For larger species, it can be effective by applying it directly to the gill area.

Synonyms: Tricaine methanesulfonate, Tricaine mesylate, TMS, MS-222, 3-aminobenzoic acid ethyl ester methanesulfonate, Ethyl M-aminobenzoate, anesthetic.

Use this space to describe how Tricaine Methanesulfonate (MS-222) is used in the research process.

Process

Write the steps to the process that uses Tricaine Methanesulfonate MS-222).

Potential Hazards

  • Irritant, corrosive to eyes, respiratory system, and skin
  • Retinal toxicity has been reported for workers with a history of long-term skin exposure
  • The lethal dose when delivered intravenously to mice was 180 mg/kg (LD50).

Use this space to provide details about the potential hazards of Tricaine Methanesulfonate (MS-222)as it pertains to your research protocol.

Engineering Controls

  • Use of chemical fume hood to prepare a concentrated stock solution by mixing an appropriate amount of Tricaine Methanesulfonate (MS-222) powder in a small volume of water. Alternatively use a top loading balance with a clear plastic wind guard.
  • Laboratories and other spaces where handling of Tricaine Methanesulfonate (MS-222) occurs must be equipped with an eyewash station.

Use this space to provide details about the engineering controls to use when working with Tricaine Methanesulfonate (MS-222)as it pertains to your research protocol.

Work Practice Controls

  • Designate areas where Tricaine Methanesulfonate (MS-222) is stored or manipulated.
  • The solution of Tricaine Methanesulfonate (MS-222) needs to be prepared fresh each time because it is light-sensitive and might form toxic by-products upon exposure to light.
  • Wear nitrile gloves and use a utensil to stir until all powder is dissolved.
  • Wear nitrile gloves to handle animals exposed to MS-222 to prevent systemic absorption.
  • Decontamination should consist of surface cleaning with water and detergent followed by thorough rinsing. The use of detergent is recommended because there is no single accepted method of chemical deactivation for all agents involved. A plastic backed absorbent pad should be placed under the work area during preparation. This should be changed following use or when a spill occurs.

Use this space to provide additional details about workpractice controls for Tricaine Methanesulfonate (MS-222)as it pertains to your research protocol.

Personal Protective Equipment

Personal protective equipment should include a laboratory coat, goggles and nitrile gloves. A N95 disposable respirator should be worn if weighing material outside of a fume hood or enclosed balance to ensure workers are not accidently exposed to aerosolized Tricaine Methanesulfonate (MS-222). Under these circumstances, researchers will need to participate in the University of Michigan Respiratory Protection Program.

Use this space to provide details about the PPE that must be worn when working with Tricaine Methanesulfonate (MS-222)as it pertains to your research protocol.

Transportation and Storage

  • Keep containers of Tricaine Methanesulfonate (MS-222) tightly closed and stored in a well-ventilated place.
  • Check SDS for specific storage requirements.
  • Tricaine Methanesulfonate (MS-222) is not regulated by DOT as a Hazardous Material.

Use this space to provide details about the transportation and storage requirements for Tricaine Methanesulfonate (MS-222)as it pertains to your research protocol.

Waste Disposal

Do not discard Tricaine Methanesulfonate (MS-222)directly into surface water, storm water conveyances, or catch basins.

Sharps – place needles, syringes with needles attached and other breakable items into appropriately labeled sharps containers.

Empty stock vials, reagent bottles, etc. – triple rinse with copious amounts of water. Deface label with black magic marker or scraper. Place in a cardboard box for disposal.

Because most spent, unused, and expired chemicals/materials are considered hazardous wastes, they must be properly disposed of. Do not dispose of chemical wastes by dumping them down a sink, flushing in a toilet or discarding in regular trash containers, unless authorized by Environment, Health & Safety (EHS) Hazardous Materials Management (HMM). Contact EHS-HMM at (734) 763-4568 for waste containers, labels, manifests, waste collection and for any questions regarding proper waste disposal. Also, refer to the EHS Hazardous WasteWeb page for more information.

Use this space to provide details about the waste disposal requirements for Tricaine Methanesulfonate (MS-222).

Exposures/Unintended Contact

If the employee is in need of emergency medical attention, call 911 immediately.

For a chemical exposure/injury:

injury type / action / notes
Exposure-Eyes /
  1. Wash out immediately with fresh running water for at least 15 minutes.
  2. Ensure complete irrigation of the eye by keeping eyelids apart and away from eye and moving the eyelids by occasionally lifting the upper and lower lids.
  3. If pain persists or recurs, seek medical attention.
/ Removal of contact lenses after an eye injury should only be undertaken by skilled personnel.
Exposure-Skin /
  1. Immediately remove all contaminated clothing, including footwear.
  2. Flush skin and hair with running water (and soap if available) for at least 15 minutes.
  3. Seek medical attention in event of irritation.

Inhalation (including spills of powder outside of a chemical fume hood) /
  1. Remove patient from contaminated area.
  2. Seek medical attention.

Ingestion / Seek medical advice.
Do NOT induce vomiting, unless directed to do so by medical personnel.
If vomiting occurs, lean patient forward or place on left side (head-down position, if possible) to maintain open airway and prevent aspiration. / Never give liquid to a person showing signs of being sleepy or with reduced awareness; i.e. becoming unconscious.
NOTE: If an ambulance is needed, call the University of Michigan Division of Public Safety and Security (DPSS) at 911 to request assistance.

Contact EHS for advice on symptoms of chemical exposure, or assistance in performing an exposure assessment.

Report all work related accidents, injuries, illnesses or exposures to Work Connectionswithin 24 hoursby completing and submitting theIllness and Injury Report Form. Follow the directions on the Work Connections websiteWhere to go for treatmentto obtain proper medical treatment and follow-up.

Complete the Incident and Near-Miss Report form.

Use this space to write instructions about how to respond to an exposure or unintended contact with Tricaine Methanesulfonate (MS-222).

Treatment Facilities

U-M Occupational Health Services -- Campus Employees
Mon-Fri 7:30 am - 4:30 pm
After hours - go to U-M Hospital Emergency Dept. -- Urgent Care Clinic
380 Med Inn building
1500 East Medical Center Drive, Ann Arbor (734) 764-8021

University Health Services -- University students (non-life threatening conditions)
Mon-Fri 8 am - 4:30 pm, Sat 9 am - 12 pm
Contact for current hours, as they may vary
207 Fletcher Street, Ann Arbor (734) 764 - 8320

UMHS Emergency Department -- after clinic hours or on weekends
1500 East Medical Center Drive, Ann Arbor (734) 936-6666

Spill Procedure

  • When a spill occurs, personal safety should always come first.
  • Alert and clear everyone in the immediate area where the spill occurred.

For additional information regarding spill response procedures, refer to the EHS Hazardous Waste Spill ResponseWeb page.

Minor Chemical Spill

A minor (small) chemical spill is one that the laboratory staff is capable of handling safely without the assistance of safety and emergency personnel, i.e., (less than 1 Gallon or 3.5 Liters, inside a fume hood). In the event of a minor chemical spill, use the following information for a safe cleanup process.

  • Alert people in immediate area of spill.
  • If spilled material is flammable, turn off ignition and heat sources. Don’t light Bunsen burners or turn on other switches.
  • Open outside windows, if possible.
  • Wear protective equipment, including safety goggles, gloves and long-sleeve lab coat.
  • Avoid breathing vapors from spill.
  • Confine spill to as small an area as possible.
  • Do not wash spill down the drain.
  • Use appropriate spill kits/sorbents to neutralize corrosives and/or absorb spill. Collect contaminated materials and residues and place in container. For powdered chemicals sweep carefully to avoid generation of dust or, if appropriate, use moist sorbent pads or wet the powder with a suitable solvent and then wipe with a dry cloth. Contact EHS-HMM (734) 763-4568 for proper disposal.
  • Clean spill area with water.

Major Chemical Spill

Report large chemical spills greater than 1 Gallon or 3.5 Liters in corridors or common areas, e.g., hallways, elevators, eating areas, rest rooms, offices, etc., to U-MDivision of Public Safety and Security (DPSS) by calling 911.

A major (large) chemical spill requires active assistance from emergency personnel. Do not attempt to clean up a tricaine Methanesulfonate (MS-222) spill that occurs outside a fume hood, or a major spill. In the event of a major chemical spill, use the following information for a safe spill response process.

  • Attend to injured or contaminated persons and remove them from exposure.
  • Alert people in the laboratory to evacuate.
  • If spilled material is flammable, turn off ignition and heat sources. Don’t light Bunsen burners or turn on other switches.
  • Call University of Michigan Division of Public Safety and Security (DPSS) at 911 immediately for assistance.
  • Close doors to affected area.
  • Post warnings to keep people from entering the area.
  • Have person available that has knowledge of incident and laboratory to assist emergency personnel.

Use this space to write instructions about how to respond to a spill of Tricaine Methanesulfonate (MS-222)in your work location.

Emergency Reporting

Report all emergencies, suspicious activity, injuries, spills, and fires to the University of Michigan Police (DPSS) by calling 911 or texting 377911. Register with theUniversity of Michigan Emergency Alert Systemvia Wolverine Access.

Training of Personnel

All personnel are required to complete the General Laboratory Safety Training session (BLS025wor equivalent) via the EHS My LINC Web page. Furthermore, all personnel shall read and fully adhere to this SOP when handling Tricaine Methanesulfonate (MS-222).

Certification

I have read and understand the above SOP. I agree to contact my Lab Director if I plan to modify this procedure.

Name / Signature / UMID # / Date
Laboratory Director / Revision Date

Major Revisions (Tracking purposes only -- Do not print as part of SOP)

03-26-2018Put into EHS format, changed department name, and fixed links.
Revised Spill Procedure section (AKJ).

04-09-18Revised formatting (AKJ).

04-24-18Changed injury type and action from paragraph to table format (AKJ).

05-02-18Revisions per meeting with Jon (AKJ).

Tricaine Methanesulfonate (MS-222)Page 1 of 8

Revision Date: 05/02/2018