Supplemental Tables

Supplemental Table S1.Parameters of the luciferase activity oscillation driven by the LHY promoter in the Arabidopsis seedlings under different light conditions and copper treatments.

The average period, amplitude and phase of the oscillations shown in Figure 9 and Supplemental Figure S6 with the corresponding standard errorof the mean was calculated with the BRASSanalysis package (. n, number of replicates. Asterisks indicate statistically significant differences with respect to control (MS), except for S6B (H2O) and S6C (Cu) (*P < 0.05; **P < 0.01).

Figure / Period / Amplitude / Phase / n
9A
MS / 25.31 ± 0.81 / 0.0108 ± 0.0026 / -0.82 ± 1.08 / 7
5 µM Cu / 25.25 ± 0.69 / 0.0087 ± 0.0025 / -2.39 ± 0.83 / 5
10 µM Cu / 24.16 ± 0.83 / 0.0078 ± 0.0033 / -3.69 ± 1.28 / 4
9B
MS / 24.15 ± 0.24 / 0.0038 ± 0.0003 / -3.13 ± 0.41 / 10
Cu / 24.02 ± 0.49 / 0.0023 ± 0.0002** / -3.20 ± 0.69 / 7
S6A
MS / 25.13 ± 0.04 / 0.0079 ± 0.0007 / -1.41 ± 0.19 / 23
Cu / 25.12 ± 0.05 / 0.0057 ± 0.0005* / -2.04 ± 0.25* / 21
S6B
H2O (0 h) / 24.18 ± 0.97 / 0.0061 ± 0.0007 / -4.45 ± 0.38 / 4
10 µM Cu (3h) / 24.72 ± 0.21 / 0.0045 ± 0.0010 / -2.76 ± 0.24** / 5
10 µM Cu (6h) / 25.39 ± 0.30 / 0.0052 ± 0.0008 / -2.43 ± 0.26** / 6
S6C
Cu / 24.36 ± 0.48 / 0.0088 ± 0.0007 / -3.16 ± 0.51 / 4
BCS / 24.71 ± 0.14 / 0.0137 ± 0.0018* / -2.40 ± 0.28 / 4

Supplemental Table S2. Oligonucleotides used in the RT-PCR reactions.

Th, Hybridization Temperature.

Primer name / Primer sequence / PCR conditions
18S forward
18S reverse / 5’ TGGGATATCCTGCCAGTAGTCAT
5’ CTGGATCCAATTACCAGACTCAA / 20 cycles
Th55ºC
CCA1 forward
CCA1 reverse / 5’ GTAGCTATGGGTCAAGCGCT
5’ CGACTGATAATCTCCTGCAA / 40 cycles
Th51ºC
COPT1 forward
COPT1 reverse / 5’ CAATGGATCCATGAACGAAGG
5’ CCTGAGGGAGGAACATAGTTAG / 30 cycles
Th60ºC
COPT2 forward
COPT2 reverse / 5’ ACGTGTCAGTGGCTCAACC
5’ GACGGCGGAAGAAGCTCGGCGG / 30 cycles
Th60ºC
COPT3 forward
COPT3 reverse / 5’GATCTTCTCCGGCGGCTCCGG
5’GTGACTGTTACTGCTAGTGGC / 30 cycles
Th55ºC
CSD1 forward
CSD1 reverse / 5’ CAGCAGTGAGGGTGTTACG
5’ GCCCTGGAGACCAATGATG / 40 cycles
Th55ºC
CSD2 forward
CSD2 reverse / 5’ GCTGCCACCAACACAATCC
5’ GAGCGGCGTCAAGCCAATC / 40 cycles
Th55ºC
FSD1 forward
FSD1 reverse / 5’ GCTTCAAGTGCTGTCACC
5’ CAAGCCAGGCCCAGCCAG / 40 cycles
Th51ºC
LHY forward
LHY reverse / 5’ TTGAATCAGGCGTTCTTGGA
5’ TACCATACCTGAGGGATGAT / 40 cycles
Th50ºC

Supplemental Table S3. Oligonucleotides used in the real-timeRT-PCR reactions.

Primer name / Primer sequence
CCA1 q forward
CCA1 q reverse / 5’ GTTGCAGCTGCTAGTGCTTG
5’ GAAGATCGAGCCTTTGATGC
COL1 q forward
COL1 q reverse / 5’ ACAGTTCCACGGCCACTAAC
5’ CCAATCGAGAAGCCATTGTT
COPT1 q forward
COPT1 q reverse / 5’ TTGCAATTTTCCTCTCCTCCCAA
5’ ATGATGGTCGAGGCATT
COPT2 q forward
COPT2 q reverse / 5’ CCTTTCGTATTTGGTGATGCT
5’ AAACACCTGCGTTAAAGGAC
COPT3 q forward
COPT3 q reverse / 5’ TATTACAGACTGCGGTTTAC
5’ CGAAGACTCCTCCATTGAAC
FSD1 q forward
FSD1 q reverse / 5’ ACCGAAGACCAGATTACATA
5’ TGGCACTTACAGCTTCCCAA
LHCB1.1 q forward
LHCB1.1 q reverse / 5’ TTCCCTGGAGACTACGGATG
5’TCCAAACTTGACTCCGTTCC
LHY q forward
LHY q reverse / 5’ TCGGCCTCTTCTTCACAGTT
5’ ACACCCGAGCAATTCTCATC
UBQ10 q forward
UBQ10 q reverse / 5’TAATCCCTGATGAATAAGTGTTCTAC
5’AAAACGAAGCGATGATAAAGAAG