<TC>Supplemental Table 1. Partial Pearson’s Correlation Matrix, Showing Pearson’s Correlation Coefficients (r), Associated Probabilities (P), and Sample Sizes (n), for Selected Variables in Sarasota Bay, FL, Bottlenose Dolphinsa

<TCH> / Age / GSH-Px / Seblood / Seserum / log10THgblood / log10THgepidermis / δ15Nblood / δ13Cblood
<TB>Seblood / 0.44 / 0.38 / 1
0.005 / 0.022
39 / 36
Seserum / 0.554 / 0.225 / 0.361 / 1
0.001 / 0.194 / 0.033
32 / 35 / 35
log10THgblood / 0.771 / 0.308 / 0.33 / 0.65 / 1
<0.001 / 0.067 / 0.025 / <0.001
48 / 36 / 46 / 35
log10THgepidermis / 0.785 / 0.206 / 0.355 / 0.621 / 0.917 / 1
<0.001 / 0.228 / 0.016 / <0.001 / <0.001
47 / 36 / 46 / 35 / 50
δ15Nblood / 0.3 / 0.321 / 0.185 / −0.268 / 0.452 / 0.365 / 1
0.04 / 0.06 / 0.224 / 0.126 / 0.001 / 0.01
47 / 35 / 45 / 34 / 54 / 49
δ13Cblood / −0.231 / −0.355 / -0.269 / 0.204 / −0.27 / −0.255 / −0.685 / 1
0.118 / 0.036 / 0.074 / 0.247 / 0.048 / 0.077 / <0.001
47 / 35 / 45 / 34 / 54 / 49 / 54
δ15Nepidermis / 0.206 / −0.02 / 0.017 / −0.18 / 0.294 / 0.256 / 0.517 / −0.228
0.169 / 0.91 / 0.914 / 0.308 / 0.04 / 0.064 / <0.001 / 0.119
46 / 35 / 45 / 34 / 49 / 53 / 48 / 48
δ13Cepidermis / 0.002 / −0.321 / −0.067 / 0.137 / −0.104 / −0.062 / −0.322 / 0.664
0.987 / 0.06 / 0.66 / 0.438 / 0.478 / 0.66 / 0.026 / <0.001
46 / 35 / 45 / 34 / 49 / 53 / 48 / 48

<TFN>aSignificant (P < 0.05) correlations are in bold.

<TC>SupplementalTable 1 (continued). Partial Pearson’s Correlation Matrix, Showing Pearson’s Correlation Coefficients (r), Associated Probabilities (P) and Sample Sizes (n) for Selected Variables in Sarasota Bay, FL, Bottlenose Dolphinsa

<TCH> / Age / GSH-Px / Seblood / Seserum / log10THgblood / log10THgepidermis / δ15Nblood / δ13Cblood / δ13Cepidermis / T3 / T4 / T3/T4 / log10(free T4 + 1) / TIBC / Glucose / ALT
<TB>T3 / −0.415 / −0.38 / −0.267 / −0.355 / −0.362 / −0.419 / −0.057 / 0.038 / −0.031 / 1
0.004 / 0.035 / 0.091 / 0.054 / 0.01 / 0.003 / 0.698 / 0.798 / 0.833
47 / 31 / 41 / 30 / 50 / 49 / 49 / 49 / 48
T4 / −0.511 / −0.162 / −0.084 / −0.453 / −0.39 / −0.442 / −0.089 / −0.001 / −0.062 / 0.706 / 1
<0.001 / 0.383 / 0.603 / 0.012 / 0.005 / 0.001 / 0.545 / 0.992 / 0.675 / <0.001
47 / 31 / 41 / 30 / 50 / 49 / 49 / 49 / 48 / 54
T3/T4 / −0.162 / −0.481 / −0.297 / −0.072 / −0.216 / −0.22 / −0.02 / 0.056 / 0.013 / 0.816 / 0.188 / 1
0.278 / 0.006 / 0.059 / 0.706 / 0.131 / 0.129 / 0.893 / 0.704 / 0.929 / <0.001 / 0.172
47 / 31 / 41 / 30 / 50 / 49 / 49 / 49 / 48 / 54 / 54
log10(free T4 + 1) / −0.386 / −0.313 / −0.189 / −0.117 / −−0.391 / −0.379 / −0.36 / 0.424 / 0.077 / 0.305 / 0.45 / 0.079 / 1
0.007 / 0.087 / 0.238 / 0.537 / 0.005 / 0.007 / 0.011 / 0.002 / 0.603 / 0.025 / 0.001 / 0.569
47 / 31 / 41 / 30 / 50 / 49 / 49 / 49 / 48 / 54 / 54 / 54
TIBC / −0.448 / −0.568 / −0.323 / −0.184 / −0.357 / −0.29 / −0.309 / 0.419 / 0.355 / 0.41 / 0.221 / 0.365 / 0.344 / 1
0.001 / <0.001 / 0.029 / 0.289 / 0.007 / 0.034 / 0.023 / 0.002 / 0.009 / 0.002 / 0.108 / 0.007 / 0.011
52 / 36 / 46 / 35 / 55 / 54 / 54 / 54 / 53 / 54 / 54 / 54 / 54
Glucose / −0.476 / −0.563 / −0.515 / −0.359 / −0.434 / −0.483 / −0.184 / 0.444 / 0.3 / 0.33 / 0.058 / 0.4 / 0.306 / 0.451 / 1
<0.001 / <0.001 / <0.001 / 0.034 / 0.001 / <0.001 / 0.183 / 0.001 / 0.029 / 0.015 / 0.679 / 0.003 / 0.024 / <0.001
52 / 36 / 46 / 35 / 55 / 54 / 54 / 54 / 53 / 54 / 54 / 54 / 54 / 59
log10ALP / −0.665 / −0.573 / −0.381 / −0.319 / −0.397 / −0.436 / −0.112 / 0.256 / 0.118 / 0.596 / 0.501 / 0.438 / 0.534 / 0.523 / 0.461
<0.001 / <0.001 / 0.009 / 0.062 / 0.003 / 0.001 / 0.421 / 0.062 / 0.407 / <0.001 / <0.001 / 0.001 / <0.001 / <0.001 / <0.001
52 / 36 / 46 / 35 / 55 / 54 / 54 / 54 / 53 / 54 / 54 / 54 / 54 / 59 / 59
ALT / 0.22 / −0.008 / 0.266 / 0.137 / 0.24 / 0.309 / −0.066 / −0.035 / 0.098 / 0.076 / 0.068 / 0.072 / 0.062 / 0.298 / −0.142 / 1
0.117 / 0.965 / 0.074 / 0.434 / 0.078 / 0.023 / 0.637 / 0.8 / 0.484 / 0.585 / 0.623 / 0.606 / 0.655 / 0.022 / 0.283
52 / 36 / 46 / 35 / 55 / 54 / 54 / 54 / 53 / 54 / 54 / 54 / 54 / 59 / 59
AST / 0.128 / 0.08 / 0.324 / 0.181 / 0.247 / 0.228 / −0.029 / −0.042 / 0.099 / 0.175 / 0.211 / 0.091 / 0.064 / 0.252 / −0.162 / 0.876
0.367 / 0.643 / 0.028 / 0.299 / 0.068 / 0.035 / 0.837 / 0.762 / 0.481 / 0.585 / 0.126 / 0.513 / 0.644 / 0.054 / 0.22 / <0.001
52 / 36 / 46 / 35 / 55 / 54 / 54 / 54 / 53 / 54 / 54 / 54 / 54 / 59 / 59 / 59

<TFN>aSignificant (P < 0.05) correlations are in bold.

<TC>Supplemental Table 2a. Adjusted Least Squares Means, Standard Errors (SE), and Sample Sizes (n), with Statistics from Two-way Analysis of Covariance (ANCOVA) for Variables Significantly (P < 0.05) Influenced by Sex, Season, and Age in Sarasota Bay, FL, Bottlenose Dolphinsa

<TCH>Dependent variable: total iron
Effect / Summer / Winter / Sex / Season / Sex,a season / Age covariate
<TB>Sex / Mean ± SE (n) / Mean ± SE (n) / F-ratio / P-value / F-ratio / P-value / F-ratio / P-value / F-ratio / P-value
F / 139 ± 11.3 (11) / 168 ± 9.4 (16) / 6.991 / 0.011 / 9.634 / 0.003 / 0.096 / 0.76 / 13.821 / 0.001
M / 107 ± 9.1 (17) / 143 ± 12.2 (10)
<TCH>Dependent variable:TIBC
Effect / Summer / Winter / Sex / Season / Sex,a season / Age covariate
<TB> Sex / Mean ± SE (n) / Mean ± SE (n) / F-ratio / P-value / F-ratio / P-value / F-ratio / P-value / F-ratio / P-value
F / 308 ± 13.8 (11) / 406 ± 11.6 (16) / 7.890 / 0.007 / 42.526 / <0.001 / 1.220 / 0.275 / 28.066 / <0.001
M / 286 ± 11.1 (17) / 356 ± 14.9 (10)
<TCH>Dependent variable: T3
Effect / Summer / Winter / Sex / Season / Sex,a season / Age covariate
<TB> Sex / Mean ± SE (n) / Mean ± SE (n) / F-ratio / P-value / F-ratio / P-value / F-ratio / P-value / F-ratio / P-value
F / 1.21 ± 0.124 (9) / 1.73 ± 0.094 (16) / 4.138 / 0.048 / 6.704 / 0.013 / 4.250 / 0.045 / 11.935 / 0.001
M / 1.67 ± 0.099 (14) / 1.73 ± 0.126 (9)

<TFN>a Tables 2a–c. For all variables of interest, effects of season and sex (with age as a covariate) were initially examined via two-way ANCOVA using data in which two adult male dolphins were represented twice, once in each season. For variables on which season had no significant effect in this initial ANCOVA, data was pooled across seasons, using mean values for these two dolphins. This pooled data then was evaluated for effect of sex via one-way ANCOVA with age as a covariate. Those parameters for which either age or sex was not significant were reanalyzed by Student’s t-test following removal of the nonsignificant effect. Variables that were significantly influenced (P < 0.05) by age, but not by either sex or season, included: Seblood, Seserum, log10THgblood, log10THgepidermis, log10MeHgblood, log10MeHgepidermis, 15δNblood, T4, and log10(free T4 + 1).

<TC>Supplemental Table 2b. Adjusted Least Squares Means, Standard Errors (SE), and Sample Sizes (n), with Statistics from One-way Analysis of Covariance (ANCOVA), for Variables Significantly (P < 0.05) Influenced by Season and Age in Sarasota Bay, FL, Bottlenose Dolphinsa

<TCH> / Summer / Winter / Season / Age covariate
Dependent variable / Mean ± SE (n) / Mean ± SE (n) / F-ratio / P-value / F-ratio / P-value
<TB>ALT (U/L) / 27.8 ± 4.38 (28) / 51.7 ± 4.54 (26) / 14.291 / <0.001 / 4.138 / 0.047
Glucose (mg/dl) / 97.8 ± 3.51 (28) / 113 ± 3.65 (26) / 8.593 / 0.005 / 15.894 / <0.001
log10ALP (U/L) / 2.32 ± 0.032 (28) / 2.48 ± 0.033 (26) / 11.718 / 0.001 / 46.441 / <0.001
GSH-Px (mU/mg Hgb) / 123.6 ± 5.96 (10) / 97.7 ± 3.84 (24) / 13.327 / 0.001 / 4.877 / 0.035

<TFN>aInitial ANCOVA showed no significant influence of sex on these variables.

<TC>SupplementalTable 2c. Arithmetic Means, Standard Errors (SE), and Sample Sizes (n) in Sarasota Bay, FL, Bottlenose Dolphins that Differed between Seasons (Sexes Pooled), via Student’s t-test (P < 0.05)a

<TCH> / Summer / Winter
Dependent variable / Mean ± SE (n) / Mean ± SE (n) / t / P-value
<TB>δ13Cblood / −14.80 ± 0.189 (32) / −14.09 ± 0.149 (24) / −2.766 / 0.008
δ13Cepidermis / −15.31 ± 0.312 (28) / −4.11 ± 0.211 (25) / −3.198 / 0.002
T3 (ng/ml)/T4 (μg/dl) / 0.111 ± 0.005 (29) / 0.137 ± 0.004 (26) / −3.993 / <0.001
AST (U/L) / 232.12 ± 8.97 (34) / 283.52 ± 16.65 (27) / −2.718 / 0.010

<TFN>aInitial ANCOVAs showed no significant influence of age or sex for these variables.