Table S3. Identification of Immunogenic Proteins by Mass Spectrometry (Spots from the 2D-Gel)

Table S3. Identification of immunogenic proteins by mass spectrometry (spots from the 2D-gel).

Molecular Weight
(kDa) / Number of unique peptides / Number of total spectra / Accession No.
(Uniprot)
Spot 1
Putative 60 kDa cysteine-rich outer membrane protein (omcB) / 56 kDa / 16 / 36 / D6YWK8_WADCW
60 kDa chaperonin 2 (hspB) / 56 kDa / 9 / 9 / D6YRQ1_WADCW
Probable cytosol aminopeptidase (pepA) / 53 kDa / 5 / 5 / D6YW21_WADCW
Glucose-6-phosphate 1-dehydrogenase (zwf) / 60 kDa / 2 / 3 / D6YWH8_WADCW
Putative membrane protein (ompA11) / 34 kDa / 2 / 2 / D6YSY8_WADCW
Na(+)-translocating NADH-quinone reductase subunit F (nqrF) / 49 kDa / 2 / 2 / D6YTY0_WADCW
Biotin carboxylase (accC) / 50 kDa / 2 / 2 / D6YVS4_WADCW
Spot 2
Putative 60 kDa cysteine-rich outer membrane protein (omcB) / 56 kDa / 8 / 37 / D6YWK8_WADCW
Transcription termination factor Rho (rho) / 51 kDa / 8 / 11 / D6YSE7_WADCW
60 kDa chaperonin 2 (hspB) / 56 kDa / 7 / 8 / D6YRQ1_WADCW
Na(+)-translocating NADH-quinone reductase subunit F (nqrF) / 49 kDa / 7 / 8 / D6YTY0_WADCW
Probable cytosol aminopeptidase (pepA) / 53 kDa / 4 / 6 / D6YW21_WADCW
Dihydrolipoamide acetyltransferase (pdhC2) / 48 kDa / 4 / 5 / D6YWP7_WADCW
60 kDa chaperonin 1 (groEL1) / 58 kDa / 4 / 4 / D6YRK0_WADCW
DO serine protease (htrA3) / 52 kDa / 3 / 4 / D6YUJ3_WADCW
Putative outer membrane efflux protein (wcw_1826) / 54 kDa / 3 / 3 / D6YSX0_WADCW
Putative uncharacterized protein (wcw_0171) / 50 kDa / 3 / 3 / D6YTT5_WADCW
Elongation factor G (fusA) / 77 kDa / 3 / 3 / D6YU70_WADCW
Glutamyl-tRNA(Gln) amidotransferase subunit A (gatA) / 53 kDa / 3 / 3 / D6YVE4_WADCW
ATP synthase subunit beta (atpD) / 51 kDa / 3 / 3 / D6YWH4_WADCW
Asparaginyl-tRNA synthetase (asnS) / 54 kDa / 2 / 2 / D6YS69_WADCW
Type III secretion cytoplasmic ATPase SctN (sctN) / 47 kDa / 2 / 2 / D6YSB6_WADCW
Putative type III secretion protein SctC (sctC) / 124 kDa / 2 / 2 / D6YSC2_WADCW
Putative cytochrome oxidase, cytochrome c subunit (fixO) / 50 kDa / 2 / 2 / D6YTA2_WADCW
Dihydrolipoyl dehydrogenase (lpdA1) / 51 kDa / 2 / 2 / D6YTL4_WADCW
Elongation factor Tu (tuf) / 43 kDa / 2 / 2 / D6YUZ2_WADCW
V-type ATP synthase beta chain (ntpB) / 48 kDa / 2 / 2 / D6YV13_WADCW
Glucose-6-phosphate isomerase (pgi) / 58 kDa / 2 / 2 / D6YVD2_WADCW
Biotin carboxylase (accC) / 50 kDa / 2 / 2 / D6YVS4_WADCW
Spot 3
Elongation factor G (fusA) / 77 kDa / 8 / 9 / D6YU70_WADCW
DNA-directed RNA polymerase subunit beta (rpoB) / 142 kDa / 6 / 6 / D6YUZ9_WADCW
Polyribonucleotide nucleotidyltransferase (pnp) / 77 kDa / 2 / 2 / D6YV93_WADCW
Spot 4
Elongation factor G (fusA) / 77 kDa / 26 / 39 / D6YU70_WADCW
Polyribonucleotide nucleotidyltransferase (pnp) / 77 kDa / 11 / 13 / D6YV93_WADCW
30S ribosomal protein S1 (rpsA) / 64 kDa / 6 / 7 / D6YVR5_WADCW
Putative membrane protein (ompA11) / 34 kDa / 3 / 3 / D6YSY8_WADCW
Tail-specific protease (tsp3) / 74 kDa / 2 / 2 / D6YSI3_WADCW
Transketolase (tkt) / 75 kDa / 2 / 2 / D6YU03_WADCW
Putative uncharacterized protein (wcw_0244) / 397 kDa / 2 / 2 / D6YU08_WADCW
Spot 6
60 kDa chaperonin 1 (groEL1) / 58 kDa / 15 / 55 / D6YRK0_WADCW
60 kDa chaperonin 3 (groEL3) / 58 kDa / 14 / 29 / D6YSZ3_WADCW
V-type ATP synthase, subunit A (ntpA) / 66 kDa / 12 / 16 / D6YV12_WADCW
General secretion pathway protein E (gspE) / 60 kDa / 7 / 12 / D6YT55_WADCW
Putative uncharacterized protein (wcw_1268) / 70 kDa / 11 / 11 / D6YWV9_WADCW
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B (gatB) / 55 kDa / 7 / 10 / D6YVE3_WADCW
DNA-directed RNA polymerase subunit beta (rpoB) / 142 kDa / 6 / 6 / D6YUZ9_WADCW
Putative K(+)-stimulated pyrophosphate-energized sodium pump 1 (hppA1) / 72 kDa / 4 / 4 / D6YVD1_WADCW
RNA polymerase sigma-54 factor (rpoN) / 56 kDa / 3 / 3 / D6YTT7_WADCW
Putative 60 kDa cysteine-rich outer membrane protein (omcB) / 56 kDa / 2 / 2 / D6YWK8_WADCW
Putative membrane protein (ompA11) / 34 kDa / 2 / 2 / D6YSY8_WADCW
Putative uncharacterized protein (wcw_0244) / 397 kDa / 2 / 2 / D6YU08_WADCW
Putative uncharacterized protein (wcw_0812) / 59 kDa / 2 / 2 / D6YVL8_WADCW
Spot 8
V-type ATP synthase beta chain (ntpB) / 48 kDa / 14 / 22 / D6YV13_WADCW
60 kDa chaperonin 2 (hspB) / 56 kDa / 11 / 11 / D6YRQ1_WADCW
Type III secretion cytoplasmic ATPase SctN (sctN) / 47 kDa / 7 / 9 / D6YSB6_WADCW
Putative uncharacterized protein (wcw_1513) / 47 kDa / 8 / 8 / D6YS17_WADCW
Putative outer membrane factor (OMF) family efflux porin (wcw_1817) / 54 kDa / 6 / 6 / D6YSW1_WADCW
Putative uncharacterized protein (wcw_0448) / 48 kDa / 6 / 6 / D6YUK9_WADCW
Putative outer membrane efflux protein (wcw_1826) / 54 kDa / 5 / 5 / D6YSX0_WADCW
Isocitrate lyase (aceA) / 48 kDa / 5 / 5 / D6YVC5_WADCW
60 kDa chaperonin 1 (groEL1) / 58 kDa / 4 / 4 / D6YRK0_WADCW
tRNA modification GTPase TrmE (trmE) / 50 kDa / 4 / 4 / D6YSS9_WADCW
Putative uncharacterized protein (wcw_1602) / 46 kDa / 3 / 3 / D6YSA2_WADCW
Putative membrane protein (ompA4) / 39 kDa / 3 / 3 / D6YTI0_WADCW
Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) / 42 kDa / 3 / 3 / D6YUI5_WADCW
Putative uncharacterized protein (wcw_0836) / 45 kDa / 3 / 3 / D6YVP1_WADCW
Putative uncharacterized protein (wcw_0244) / 397 kDa / 3 / 3 / D6YU08_WADCW
Elongation factor Tu (tuf) / 43 kDa / 2 / 2 / D6YUZ2_WADCW
Putative type III secretion protein SctC (sctC) / 124 kDa / 2 / 2 / D6YSC2_WADCW
60 kDa chaperonin 3 (groEL3) / 58 kDa / 2 / 2 / D6YSZ3_WADCW
Putative membrane protein (ompA3) / 40 kDa / 2 / 2 / D6YTH9_WADCW
Putative membrane protein (ompA11) / 34 kDa / 2 / 2 / D6YSY8_WADCW
Na(+)-translocating NADH-quinone reductase subunit F (nqrF) / 49 kDa / 2 / 2 / D6YTY0_WADCW
Putative 60 kDa cysteine-rich outer membrane protein (omcB) / 56 kDa / 1 / 2 / D6YWK8_WADCW
Spot 9
Elongation factor Tu (tuf) / 43 kDa / 14 / 19 / D6YUZ2_WADCW
ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) / 46 kDa / 3 / 3 / D6YS99_WADCW
Adenylosuccinate synthetase (purA) / 48 kDa / 2 / 2 / D6YU79_WADCW
Spot 10
Elongation factor Tu (tuf) / 43 kDa / 12 / 23 / D6YUZ2_WADCW
DNA-directed RNA polymerase subunit alpha (rpoA) / 42 kDa / 10 / 20 / D6YRY5_WADCW
S-adenosylmethionine synthase (metK) / 43 kDa / 10 / 12 / D6YTP1_WADCW
ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) / 46 kDa / 9 / 9 / D6YS99_WADCW
Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) / 42 kDa / 5 / 6 / D6YUI5_WADCW
Gamma-glutamyl phosphate reductase (proA) / 45 kDa / 5 / 5 / D6YRV7_WADCW
Putative uncharacterized protein (wcw_1632) / 50 kDa / 5 / 5 / D6YSD2_WADCW
Enolase (eno) / 47 kDa / 5 / 5 / D6YT05_WADCW
Adenylosuccinate synthetase (purA) / 48 kDa / 5 / 5 / D6YU79_WADCW
Putative 60 kDa cysteine-rich outer membrane protein (omcB) / 56 kDa / 4 / 5 / D6YWK8_WADCW
Alanine dehydrogenase (ald) / 39 kDa / 4 / 5 / D6YSC8_WADCW
6-phosphogluconate dehydrogenase, decarboxylating (gnd) / 53 kDa / 4 / 4 / D6YUB8_WADCW
Putative serine/threonine PP2C protein phosphatase (wcw_0974) / 43 kDa / 4 / 4 / D6YW23_WADCW
2-amino-3-ketobutyrate coenzyme A ligase (kbl) / 44 kDa / 3 / 3 / D6YWJ3_WADCW
Putative uncharacterized protein (wcw_1603) / 44 kDa / 2 / 3 / D6YSA3_WADCW
60 kDa chaperonin 1 (groEL1) / 58 kDa / 2 / 2 / D6YRK0_WADCW
60 kDa chaperonin 2 (hspB) / 56 kDa / 2 / 2 / D6YRQ1_WADCW
Proline amido peptidase (wcw_0579) / 45 kDa / 2 / 2 / D6YUY8_WADCW
Putative uncharacterized protein (wcw_0776) / 34 kDa / 2 / 2 / D6YVI2_WADCW
Putative uncharacterized protein (wcw_1222) / 42 kDa / 2 / 2 / D6YWR8_WADCW
Putative uncharacterized protein (wcw_1249) / 42 kDa / 2 / 2 / D6YWU5_WADCW
Spot 11
60 kDa chaperonin 2 (hspB) / 56 kDa / 27 / 149 / D6YRQ1_WADCW
Transcription elongation protein nusA (nusA) / 48 kDa / 17 / 48 / D6YVR4_WADCW
Putative 60 kDa cysteine-rich outer membrane protein (omcB) / 56 kDa / 10 / 31 / D6YWK8_WADCW
Acetyl/propionyl-CoA carboxylase, beta subunit (wcw_0447) / 57 kDa / 9 / 10 / D6YUK8_WADCW
Glutamyl-tRNA synthetase (gltX) / 58 kDa / 8 / 10 / D6YSI4_WADCW
60 kDa chaperonin 1 (groEL1) / 58 kDa / 7 / 8 / D6YRK0_WADCW
Isocitrate dehydrogenase (icd) / 54 kDa / 5 / 11 / D6YSM6_WADCW
30S ribosomal protein S1 (rpsA) / 64 kDa / 5 / 5 / D6YVR5_WADCW
Glucose-6-phosphate 1-dehydrogenase (zwf) / 60 kDa / 5 / 6 / D6YWH8_WADCW
Cysteinyl-tRNA synthetase (cysS) / 55 kDa / 4 / 4 / D6YRM5_WADCW
Transcription termination factor Rho (rho) / 51 kDa / 3 / 3 / D6YSE7_WADCW
Probable cytosol aminopeptidase (pepA) / 53 kDa / 3 / 3 / D6YW21_WADCW
Putative membrane protein (ompA4) / 39 kDa / 3 / 6 / D6YTI0_WADCW
DO serine protease (htrA3) / 52 kDa / 3 / 3 / D6YUJ3_WADCW
Prolyl-tRNA synthetase (proS) / 58 kDa / 3 / 3 / D6YUF5_WADCW
Putative K(+)-stimulated pyrophosphate-energized sodium pump 1 (hppA1) / 72 kDa / 3 / 3 / D6YVD1_WADCW
Putative membrane protein (ompA3) / 40 kDa / 2 / 2 / D6YTH9_WADCW
Ribonuclease G (cafA) / 59 kDa / 2 / 2 / D6YUG2_WADCW
Hydrolase (wcw_0511) / 60 kDa / 2 / 2 / D6YUS1_WADCW
Putative uncharacterized protein (wcw_0518) / 57 kDa / 2 / 2 / D6YUS8_WADCW
Putative membrane protein (ompA11) / 34 kDa / 2 / 3 / D6YSY8_WADCW
MOMP-like family protein (ompA2) / 39 kDa / 2 / 2 / F8LBJ9_9CHLA
Spot 17
Sodium-type flagellar protein motY (pal) / 29 kDa / 3 / 14 / D6YTN6_WADCW
Putative membrane protein (ompA11) / 34 kDa / 3 / 5 / D6YSY8_WADCW
50S ribosomal protein L28 (rpmB) / 10 kDa / 2 / 3 / D6YVB6_WADCW
30S ribosomal protein S10 (rpsJ) / 12 kDa / 2 / 2 / D6YU69_WADCW
Spot 18
Elongation factor Ts (tsf) / 31 kDa / 12 / 17 / D6YT74_WADCW
Putative membrane protein (ompA11) / 34 kDa / 7 / 7 / D6YSY8_WADCW
DNA-directed RNA polymerase subunit alpha (rpoA) / 42 kDa / 3 / 3 / D6YRY5_WADCW
Putative type III secretion translocase SctQ (sctQ1) / 32 kDa / 3 / 3 / D6YSB9_WADCW
Putative uncharacterized protein (wcw_1706) / 28 kDa / 3 / 3 / D6YSK5_WADCW
Putative uncharacterized protein (wcw_1803) / 34 kDa / 3 / 3 / D6YSU7_WADCW
Putative uncharacterized protein (wcw_1585) / 25 kDa / 2 / 2 / D6YS88_WADCW
50S ribosomal protein L1 (rplA) / 25 kDa / 2 / 3 / D6YUZ6_WADCW
Putative uncharacterized protein (wcw_0940) / 30 kDa / 2 / 2 / D6YVZ0_WADCW
Spot 19
30S ribosomal protein S14 (rpsN) / 12 kDa / 3 / 3 / D6YVK9_WADCW
30S ribosomal protein S8 (rpsH) / 15 kDa / 2 / 2 / D6YRZ3_WADCW
50S ribosomal protein L23 (rplW) / 13 kDa / 2 / 2 / D6YS04_WADCW
Putative membrane protein (ompA11) / 34 kDa / 2 / 2 / D6YSY8_WADCW
Putative uncharacterized protein (wcw_1930) / 41 kDa / 2 / 2 / D6YT71_WADCW
Spot 22
Putative uncharacterized protein (wcw_1541) / 37 kDa / 4 / 4 / D6YS45_WADCW
DNA-directed RNA polymerase subunit beta' (rpoC) / 155 kDa / 3 / 3 / D6YV00_WADCW
UDP-4-amino-4-deoxy-L-arabinose--oxoglutarateaminotransferase (arnB) / 43 kDa / 2 / 2 / D6YUA9_WADCW
UPF0365 protein wcw_1211 (yqfA) / 37 kDa / 2 / 2 / D6YWQ7_WADCW