Table S2. Templates for PCR used in the construction of pBiSt plasmids

Label in Fig. 1 / PCR product / Templates / PCR primers used for amplification / Ref.a
a / Bn / pCambia-LOX
-Bar-ProA-Bara / Sal-Bn-F + Bn-Sac-R / -
b / Bs / Sal-Bs-F + Bs-Sac-R / -
c / NCPA / pBI121
pE4Pin
Products of 1 / 1.  NCPA-F + NCPA-JR
NCPA-JF + NCPA-R
  1. NCPA-F + NCPA-R
/ 1
2
d / XaBsTX / pRV112AG
pCambia-LOX
-Bar-ProA-Barb
Products of 1
Total DNA from N. tabacum
Clone of 4 /
  1. Prrn-F + aadA-RBS
    RBS-barstar + Barstar-BH
2.  Prrn-F + Barstar-BH (= aBs)
3.  TpsbA-F + TpsbA-R
4.  ligation of 2 and 3 (= aBsT)
  1. XaBsTX-F + TpsbA-Xho
/ 3
-
4
e / eNpt II / pX6-GFP
Products of 1 /
  1. Pnos-F + Ntp-pTrS-R
    and pTrS-F + pTrS-R
  2. Pnos-F + pTrS-R
/ 5
f / pAtDMC1 / 1.  Total DNA from A. thaliana ecotype Landsberg erecta
  1. Products of 1
/
  1. AtDMC1-F + AtDMC1dER
    and AtDMC1dEF + AtDMC1-R
  2. AtDMC1-F + AtDMC1-R
/ 6
g / pACT2 /
  1. ACT2F + ACT2dER, and ACT2dEF + ACT2R
  2. ACT2F + ACT2R
/ 7
h / BsT / PBSaBsTc
Products of 1 / 1.  Prrn-F + Prrn-RBS
RBS-Barstar+ TpsbA-R
2.  Prrn-F + TpsbA-R

a, References

b, a gift from M. Pooggin, unpublished.

c, pBS harboring aBsT (see d-4).

References

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