Table S1Lactobacilli used for bile salt hydrolase (Bsh) activity screening

Lactobacillus species or subspecies / Straina / Source of collectionb / Bsh Assaysc
Plate Assay / Ninhydrin Assayd
TDC Precipitatione / GC hydrolase activitye / TC hydrolase activitye / TDC hydrolase
Activitye
Reference Lactobacillus strains
L. johnsonii NCDO1693 / NCDO1693 / SMS, Australia / +++ / +++ / ++ / +++
L. plantarum NCDO82 / NCDO82 / SMS, Australia / +++ / +++ / ++ / +++
L. plantarum J1 / NCDC / NDRI / N / N / N / N
L. plantarum 201 / NCDC201 / NDRI / N / ++ / N / +
L. acidophilus LA1 / CSCC2401 / SMS, Australia / +++ / +++ / ++ / +++
L. acidophilus 16 / NCDC16 / Chr. Hansen Lab. / +++ / +++ / + / +++
L. acidophilus 11 / ATCC832 / Rat faeces / ++ / ++ / ++ / ++
L. acidophilus 195 / Strain R / Silage / +++ / +++ / ++ / +++
L. acidophilus 291 / LA5 / NCDC, NDRI / +++ / +++ / ++ / +++
L. acidophilus 13 / NDRIII / NCDC, NDRI / N / + / N / +
L. acidophilus 14 / ATCC11975 / NCDC, NDRI / N / ++ / + / ++
L. acidophilus 15 / NCDC / NCDC, NDRI / N / + / N / +
L. acidophilus 4962 / ATCC4962 / SMS, Australia / + / ++ / + / ++
L. fermentum 141 / NCDO394 / Infant intestine / + / ++ / + / ++
L. fermentum 155 / ATCC8289 / NCDC, NDRI / N / + / N / ++
L. fermentum 156 / ATCC9338 / NCDC, NDRI / N / + / N / ++
L. fermentum 214 / NCDO215 / NCDC, NDRI / N / N / N / N
L. rhamnosus 18 / ATCC7469 / NCDC, NDRI / N / N / N / N
L. rhamnosus 24 / ATCC8014 / NCDC, NDRI / N / N / N / N
L. rhamnosus J3 / NCDC / NCDC, NDRI / N / N / N / N
L. rhamnosus GG / ATCC 53103 / Intestinal Tract / N / N / N / N
L. helveticus 5 / ATCC8018 / NCDC, NDRI / N / N / N / N
L. helveticus 194 / NCDC194 / Chr. Hansen Lab. Denmark / N / N / N / N
L. helveticus 288 / DSM20075 / NCDC, NDRI / N / ++ / N / ++
L. helveticus 292 / NCDC292 / NCDC, NDRI / N / ++ / N / +
L. casei J13 / NCDC / NCDC, NDRI / N / N / N / N
L. casei 279 / ASCC279 / SMS, Australia / N / N / N / N
L. casei 290 / ASCC290 / SMS, Australia / N / N / N / N
L. paracasei LMG1459 / CSCC5233 / SMS, Australia / N / N / N / N
L. paracasei subsp.paracasei / DSM20011 / NCDC, NDRI / N / N / N / N
L. brevis / +++ / +++ / ++ / +++
L. delbrueckii subsp. bulgaricus / ATCC11842 / Bulgarian yogurt / N / N / N / N
L. delbrueckii subsp. bulgaricus / NCDO304 / Yogurt / N / N / N / N
L. delbrueckii subsp.bulgaricus / NCDO1006 / NCDC, NDRI / N / N / N / N
Indigenous Lactobacillus isolates
L. plantarum 5 / Lp5 / Human feces / N / N / N / N
L. plantarum 6 / Lp6 / Buffalo Milk / N / N / N / N
L. Plantarum 8 / Lp8 / Buffalo Milk / N / N / N / N
L. plantarum 9 / Lp9 / Human feces / + / ++ / + / ++
L. plantarum 10 / Lp10 / Human feces / +++ / +++ / +++ / +++
L. plantarum 11 / Lp11 / Human feces / +++ / +++ / +++ / +++
L. plantarum 20 / Lp20 / Human feces / N / + / N / +
L. plantarum 21 / Lp21 / Human feces / +++ / +++ / +++ / +++
L. plantarum 23 / Lp23 / Human feces / N / N / N / N
L. plantarum 25 / Lp25 / Human feces / N / N / N / N
L. plantarum 27 / Lp27 / Human feces / ++ / ++ / ++ / +++
L. plantarum 40 / Lp40 / Human milk / N / N / N / N
L. plantarum 41 / Lp41 / Human milk / N / N / N / N
L. plantarum 43 / Lp43 / Human milk / N / N / N / N
L. plantarum 44 / Lp44 / Human milk / N / N / N / N
L. plantarum 62 / Lp62 / Human feces / N / N / N / N
L. plantarum 63 / Lp63 / Human feces / N / N / N / N
L. plantarum 65 / Lp65 / Human feces / N / N / N / N
L. plantarum 68 / Lp68 / Human feces / N / N / N / N
L. plantarum 70 / Lp70 / Human feces / N / N / N / N
L. plantarum71 / Lp71 / Human feces / N / N / N / N
L. plantarum 72 / Lp72 / Human feces / N / N / N / N
L. plantarum 73 / Lp73 / Human feces / N / N / N / N
L. plantarum 75 / Lp75 / Human feces / ++ / +++ / ++ / +++
L. plantarum76 / Lp76 / Human feces / N / N / N / N
L. plantarum77 / Lp77 / Human feces / N / + / N / +
L. plantarum78 / Lp78 / Human feces / + / ++ / ++ / ++
L. plantarum82 / Lp82 / Human feces / N / N / N / N
L. plantarum 90 / Lp90 / Human feces / +++ / ++ / ++ / +++
L. plantarum 91 / Lp91 / Human feces / +++ / +++ / +++ / +++
L. acidophilus 36 / La36 / Human feces / + / ++ / N / +
L. acidophilus 37 / La37 / Human feces / + / ++ / N / +
L. fermentum 2 / Lf2 / Buffalo Milk / N / N / N / N
L. fermentum 3 / Lf3 / Buffalo Milk / N / N / N / N
L. fermentum 7 / Lf7 / Buffalo Milk / N / N / N / N
L. fermentum 13 / Lf13 / Human feces / N / N / N / N
L. fermentum IS47 / LfS47 / Infant feces / N / ++ / + / ++
L. fermentum42 / Lf42 / Human milk / N / N / N / N
L. fermentum 60 / Lf60 / Human feces / N / N / N / N
L. fermentum 66 / Lf66 / Human feces / N / ++ / N / N
L. fermentum 69 / Lf69 / Human feces / +++ / +++ / ++ / +++
L. fermentum 79 / Lf79 / Human feces / N / ++ / N / N
L. casei 45 / Lc45 / Human milk / N / N / N / N
L. casei 64 / Lc64 / Human feces / N / N / N / N
L. casei 92 / Lc92 / Human feces / N / N / N / N
L. casei 93 / Lc93 / Human feces / N / N / N / N
L. casei 94 / Lc94 / Human feces / N / N / N / N
L. casei 100 / Lc100 / Human feces / N / N / N / N
L. casei 101 / Lc101 / Human feces / N / N / N / N
L. paracasei 80 / Lpar80 / Human feces / N / N / ++ / ++
L. paracasei 85 / Lpar85 / Human feces / N / N / N / N
L. paracasei 87 / Lpar87 / Human feces / N / N / N / N
L. reuteri50 / Lrut50 / Human feces / N / + / N / N
L. reuteri51 / Lrut51 / Human feces / N / ++ / N / N
L. reuteri53 / Lrut53 / Human feces / N / + / N / N
L. reuteri56 / Lrut56 / Human feces / N / N / N / N
Indigenous Lactobacillus isolates of unidentified species
Lactobacillus sp 4 / Lb04 / Buffalo Milk / N / N / N / N
Lactobacillus sp12 / Lb12 / Human feces / N / N / N / N
Lactobacillus sp19 / Lb19 / Human Milk / N / N / N / N
Lactobacillus spS9 / LbS9 / Infant feces / N / N / N / N
Lactobacillus spS10 / LbS10 / Infant feces / N / N / N / N
Lactobacillus spS40 / LbS40 / Infant feces / N / ++ / N / +
Lactobacillus sp52 / Lb52 / Human feces / N / N / N / N
Lactobacillus sp54 / Lb54 / Human feces / N / N / N / N
Lactobacillus sp55 / Lb55 / Human feces / N / N / N / N
Lactobacillus sp57 / Lb57 / Human feces / N / N / N / N
Lactobacillus sp 58 / Lb58 / Human feces / N / N / N / N
Lactobacillus sp59 / Lb59 / Human feces / N / N / N / N
aNCDO, National Collection of Dairy Organisms; ATCC, American Type Culture Collection; NCDC, National Collection of Dairy Cultures; ASCC, Australian Starter Culture Collection Centre; La, L. acidophilus; Lj, L. johnsonii; Lp, L. plantarum; Lrut, L. reuteri; Lf, L. fermentum; Lc, L. casei; L. par, L. paracasei.
bMBU, NDRI, Molecular Biology Lab of National Dairy Research Institute; SMS, School of Molecular Sciences, Australia.
cQualitative (Direct plate assay) and quantitative (resting cell Bsh assay) bile salt hydrolase assays were used for the screening.
d Substrate specific bile salt hydrolyzing ability against glycocholate (GC), taurocholate (TC) and taurodeoxycholate (TDC). One unit of Bsh activity (U/ml) in respect to individual substrate, was defined as the amount of enzyme that liberated 1 nmol of amino acid from substrate per min per ml of the test cultures.
e Bases on the intensity of TDC precipitation or enzymatic activity units lactobacilli were categorized as High (+++), Medium (++), Low ((+) and Negative (N) Bsh producer.