Supplemental Tables
Table S1.The primers used for real-time RT-PCR.
Gene / Primer orientation / Primer sequence 5 '→3'POD (X58396) / Forward / TGCTTTGGTCTTATCGCT
Reverse / GTTTGCCTCATTCTCGTT
APX (AJ006358) / Forward / AGGGAGGGAGGACAAGC
Reverse / ACCAGAGAGGGCAACAA
CAT (U20777) / Forward / TGAAGTGCCTCTTGGAT
Reverse / AGACGGTGCCTTTGGGT
MnSOD (AY573576) / Forward / GCCATCAARTTCAACGGCGGMGGYCA
Reverse / TAGTASGCRTGYTCCCARACATC
CuZn-SOD (AY970822) / Forward / CGATAGCCAGATTCCTTTGA
Reverse / AGTCTTCCAGCATTTC
Table S2. The proline and ABA content of five barley genotypes under 4% SMC.
Genotypes / Proline content (µmol/g) / ABA content (ng/g)Control / Drought / Control / Drought
XZ5 / 46.85a / 69.07*e / 303.6e / 1054.5*b
XZ150 / 34.73b / 1522.05*b / 523.5c / 1333.9*a
ZAU3 / 30.20d / 1540.02*a / 367.8d / 699.9*d
XZ54 / 33.92c / 1421.04*c / 557.5b / 617.2*e
XZ147 / 24.95e / 1307.41*d / 702.0a / 752.6*c
Note: The data was means of three independent replicates for proline and ABA contents. *, represents significant differences (P 0.05) between drought treatment and control for each genotype. Different letters indicate significant differences (P 0.05) among 5 genotypes under control or drought condition, respectively.
Supplemental Figure
Fig.S1Shoot biomass of four wild barley genotypes and cvZAU3after10 day recovery. Different letters indicate significant differences (P ＜0.05) among two treatments and 5 genotypes. The bar represents the standard error.
Fig.S2Photosynthesis rate (Pn, A), transpiration rate(Tr, B) and stomatal conductance (Sc, C) in leaves of four wild barley genotypes and cvZAU3 after 10 dayrecovery. Different letters indicate significant differences (P ＜0.05) among two treatments and 5 genotypes.The bar represents the standard error.
Fig.S3Effect of drought stress and rewatering on MDA content in leaves of four wild barley genotypes and cvZAU3,expressed as reduced (-)/increased (+) percentage of control (%).D1, D10, D30 and D40 correspond to being exposed to drought stress for 1(30% SMC), 10 (15% SMC), 30 days(4% SMC)and rewatering for 10 days (30% SMC). Different letters indicate significant differences (P<0.05) amongthe 5 genotypes and within each sampling date.The bar represents the standard error.
Fig.S4Effect of drought stress and rewatering on SOD (A), POD (B), CAT (C) and APX(D) activity in leaves of four wild barley genotypes and cvZAU3,expressed as reduced (-)/increased (+) percentage of control (%).D1, D10, D30 and D40 correspond to being exposed to drought stress for 1 (30% SMC), 10 (15% SMC), 30 days(4% SMC) and rewatering for 10 days (30% SMC).Different letters indicate significant differences (P<0.05) amongthe 5 genotypes and within each sampling date.The bar represents the standard error.