Table S1. Primers Used for PCR Amplification and Sequencing of B-RAF

Table S1. Primers used for PCR amplification and sequencing of B-RAF

Primer / Sequence / Annealing Temperature
(°C) / Amplicon Size
(bp)
B-RAF Exon 15F / 5’ CACAGAGACCTCAAGAGTAA 3’ * / 58°C / 155 bp
B-RAF Exon 15R / 5’ ATGACTTCTGGTGCCATCCA 3’

Abbreviations: F, forward; R, reverse.

*Primer used for sequencing reaction.

Table S2. Expression levels of thyroid-specific genes in normal and tumor thyroid tissues.

Gene / Normal tissue / PTC
(n=36) / Low Risk§
(n=17) / Intermediate Risk§
(n=19) / BRAF-wt
(n=15) / BRAF-V600E
(n=21) / P value
NIS / 1 / ± / 1.121 / 0.038 / ± / 0.047 / 0.044 / ± / 0.059 / 0.031 / ± / 0.027 / 0.048 / ± / 0.060 / 0.029 / ± / 0.025 / <0.0001a
0.9266b
0.6625c
TPO / 1 / ± / 0.628 / 0.096 / ± / 0.196 / 0.159 / ± / 0.266 / 0.036 / ± / 0.046 / 0.184 / ± / 0.277 / 0.032 / ± / 0.036 / <0.0001a
0.0259b
0.0029c
Tg / 1 / ± / 0.470 / 0.366 / ± / 0.353 / 0.470 / ± / 0.409 / 0.268 / ± / 0.267 / 0.431 / ± / 0.438 / 0.379 / ± / 0.277 / <0.0001a
0.0535b
0.3953c
TSH-R / 1 / ± / 0.719 / 0.626 / ± / 0.442 / 0.677 / ± / 0.483 / 0.578 / ± / 0.407 / 0.621 / ± / 0.415 / 0.699 / ± / 0.500 / 0.0115a
0.5415b
0.9867c

For each gene, expression levels are represented as mean  standard deviation. Intergroup differences were assessed with the Mann–Whitney nonparametric test.

§The 2009 American Thyroid Association risk stratification staging system

a PTC vs. normal tissues.

b Intermediate Risk PTCs vs. Low Risk PTCs.

c BRAF-V600E PTCs vs. BRAF-wt PTCs.

Table S3. Expression levels of miRNAs in normal and tumor thyroid tissues.

miRNA / Normal tissue / PTC
(n= 21) / Low Risk§
(n=10) / Intermediate Risk§
(n= 11) / BRAF-wt
(n= 8) / BRAF-V600E
(n= 13) / P value
miR-21 / 1 / ± / 0.706 / 5.476 / ± / 5.212 / 4.592 / ± / 5.304 / 6.279 / ± / 5.246 / 4.861 / ± / 5.259 / 5.854 / ± / 5.360 / 0.0007a
0.2178b
0.587c
miR-146a / 1 / ± / 0.528 / 2.062 / ± / 2.237 / 1.325 / ± / 1.616 / 2.731 / ± / 2.574 / 2.460 / ± / 2.037 / 1.816 / ± / 2.398 / 0.4972a
0.1300b
0.2937c
miR-181a / 1 / ± / 0.650 / 3.204 / ± / 2.261 / 3.107 / ± / 2.245 / 3.293 / ± / 2.381 / 3.586 / ± / 2.424 / 2.970 / ± / 2.222 / 0.0066a
0.7513b
0.9135c
miR-221 / 1 / ± / 0.954 / 22.020 / ± / 22.927 / 23.732 / ± / 31.285 / 20.465 / ± / 12.837 / 34.776 / ± / 30.905 / 14.171 / ± / 11.985 / <0.0001a
0.5035b
0.1803c

For each miRNA, expression levels are represented as mean  standard deviation. Intergroup differences were assessed with the Mann–Whitney nonparametric test.

§The 2009 American Thyroid Association risk stratification staging system

a PTC vs. normal tissues.

b Intermediate Risk PTCs vs. Low Risk PTCs.

c BRAF-V600E PTCs vs. BRAF-wt PTCs.