Table S1 Primer combinations used to construct Y2H systems for GA and JA-Ile receptor complexes

Forward primer (5’ to 3’) / Reverse primer (5’ to 3’)
DBA-GID1a / GGATCCGTATGGCTGCGAGCGATGAAGTTA / CTGCAGTTAACATTCCGCGTTTACAAACGC
AD-GAI / GGATCCGTATGAAGAGAGATCATCATCATCATCATCAT / CTGCAGCTAA TTGGTGGAGAGTTTCCAAGCCGAGGTGG
DBD-COI1 / CCCGGGGATGGAGGATCCTGATATCAAGAGGTG / GTCGACTCATATTGGCTCCTTCAGGACTCTAAC
AD-ZAJ3 / GAATTCATGGAGAGAGATTTTCTCGGGTTG / GTCGACTTAGGTTGCAGAGCTGAGAGAAGA

Underlined sequences are restriction sites used for cloning into yeast vectors.

Table S2 Primer combinations used to amplify NPF cDNAs

AGI code / Other name / Forward primer (5’ to 3’) / Reverse primer (5’ to 3’)
NPF1.1 / At3g16180 / CACCATGGAGAACCCTCCGGATCA / ATGATCAACCAATTGGTCTT
NPF1.2 / At1g52190 / CACCATGGAGAACCCTCCCAATGA / TGCAAGACCTATTGTTCCTT
NPF1.3 / At5g11570 / aaaaagcaggctATGGATCAAGAAGCTCTTCTC / agaaagctgggtCACTTGTTATATTTATATACTACATTTACC
NPF2.1 / At3g45720 / aaaaagcaggctATGGCTGGTTTAGTATTATCTAATG / agaaagctgggtGTTGTGTCTTAGTTTGTAACATC
NPF2.2 / At3g45690 / aaaaagcaggctATGGCTCGTTCAGTCGACA / agaaagctgggtGTGTCTTAGGTTGTATCGTC
NPF2.3 / At3g45680 / CACCATGGCTAGTTTAGTCTCTGG / TTACATTGTACCATCTTTTG
NPF2.4 / At3g45700 / CACCATGGCTAATTCAGACTCTGG / ACAGAACACGTTAGTGTCCT
NPF2.5 / At3g45710 / CACCATGGCTGATTCAAAATCTGG / CTAGGTTTTAACATCTTTAG
NPF2.6 / At3g45660 / CACCATGTCTGGTTCAGTTTCCCG / TTAGTGAGAGACATTTGCAT
NPF2.7 / At3g45650 / NAXT1 / CACCATGGCTAGTTCAGTTACTGG / TCAGTGAGAGACATTTGCAG
NPF2.8 / At5g28470 / NRT1.9 / CACCATGGACGTCGAATCTTCATC / ATCTAAACATCTTTAGTGTC
NPF2.9 / At1g18880 / CACCATGGAGGTTGAGAAGACAGA / TTACACTGACACCTTATCAA
NPF2.10 / At3g47960 / GTR1 / CACCATGAAGAGCAGAGTCATTCT / TCAGACAGAGTTCTTGTCTT
NPF2.11 / At5g62680 / GTR2/NRT1.10 / aaaaagcaggctATGGAGAGAAAGCCTCTTGAAC / agaaagctgggtTCAGGCAACGTTCTTGTCTTG
NPF2.12 / At1g27080 / NRT1.6 / CACCATGGGAGTTGTTGAGAATCG / CTAACTTGGAGATTGATCGT
NPF2.13 / At1g69870 / NRT1.7 / CACCATGGTTTTGGAGGATAGAAA / TCATTTCATCGATTTCTTCG
NPF2.14 / At1g69860 / aaaaagcaggctATGGACAATGAGAAAGGGACAA / agaaagctgggtGTT TGACAT GTTGGAAATGAGG
NPF3.1 / At1g68570 / Nitr / aaaaagcaggctATGGAGGAGCAAAGCAAGAACAA / agaaagctgggtCAACGCCAAGACTCATTCATC
NPF4.1 / At3g25260 / AIT3 / N.A. / N.A.
NPF4.2 / At3g25280 / AIT4 / N.A. / N.A.
NPF4.3 / At1g59740 / NRT1.14 / N.A. / N.A.
NPF4.4 / At1g33440 / NRT1.13 / N.A. / N.A.
NPF4.5 / At1g27040 / AIT2 / N.A. / N.A.
NPF4.6 / At1g69850 / NRT1.2/AIT1 / N.A. / N.A.
NPF4.7 / At5g62730 / N.A. / N.A.
NPF5.1 / At2g40460 / CACCATGGAGGCTGCAAAAGTTTACACACAAG / TTAGATACTAAGAGGAGATGTGTCTAAGGC
NPF5.2 / At5g46050 / PTR3 / CACCATGACAGTAGAAGAGGTAGGAGACG / TTATTCAGTCTCTTTCATTTCCACCTCTTTCAC
NPF5.3 / At5g46040 / CACCATGACAGTAGAAGAGGTAGGAGATGATTATAC / TTACTCATTGTAGTTATCTACCATATTTGGTTCTTTCTG
NPF5.4 / At3g54450 / aaaaagcaggctATGGCAGATTCGACCTCCC / agaaagctgggtTGATCAAACTTATCTTTCGCTTTCAAC
NPF5.5 / At2g38100 / CACCATGTCAGTTCTGTCATGGGC / CTACACAGAAGCGATTATCC
NPF5.6 / At2g37900 / CACCATGGAGCATAAGAATATTGGTGCAGAAG / TCAGGAGAGCCCTTGGCTTG
NPF5.7 / At3g53960 / CACCATGGAGCACAACAAGGTTGATACAGAAC / TTACGTAAACTTGGACGTGTTATTCGTCC
NPF5.8 / At5g14940 / CACCATGGCTGGAGGAGAGAAAAGAAG / TTAACAATTTGTATCACATTGATCATCATCATCAC
NPF5.9 / At3g01350 / CACCATGGATTTAGAACAGAAGACAAGAGGAC / TCATTTAGGGGCTTCTTCTTTTTCATCG
NPF5.10 / At1g22540 / CACCATGTCGATCTCCGGCGCTG / TTAACTGGTGTCGAGCCTTTTAGAGAC
NPF5.11 / At1g72130 / CACCATGGCTATCACCTACTCCTCCG / TTAAAAGGTGTTTGATCTGCTGTAGAC
NPF5.12 / At1g72140 / CACCATGTCGACATCCATCGGCGATAATG / CTACTTTGGGCTGTTGTAGAGATAGGA
NPF5.13 / At1g72125 / NRT1.16 / aaaaagcaggctATGACGACGACTTCCAAAACTTC / agaaagctgggtCTACACTACGTCCACCCGG
NPF5.14 / At1g72120 / NRT1.15 / CACCATGACGACTACTTCAGAAATTTCTCTTCAAGAAG / CTACACTCGATCCACTCGACG
NPF5.15 / At1g22570 / aaaaagcaggctATGAAGATACCAGAGGAAGAAG / agaaagctgggtTTAGACTTGGTCTAGCCGAC
NPF5.16 / At1g22550 / CACCATGGCGATAGCCGAAGAAGAAGC / TTAGACTTGATCTACACGGCGATACAC
NPF6.1 / At1g13400 / N.A. / N.A.
NPF6.2 / At2g26690 / NRT1.4 / N.A.
NPF6.3 / At1g12110 / NRT1.1 / N.A. / N.A.
NPF6.4 / At3g21670 / NRT1.3 / N.A. / N.A.
NPF7.1 / At5g19640 / CACCATGGCCGCTATGGATCCG / TCAAACTTGTTCCAACTCAGAAACGG
NPF7.2 / At4g21680 / CACCATGGATCAAAAAGTTAGACAGTTTGAGG / TCAGACTTCCTCCTCTTCAGTTAC
NPF7.3 / At1g32450 / aaaaagcaggctATGTCTTGCCTAGAGATTTATAA / agaaagctgggtTTAGACTTTAGAATCCTTCTCT
NPF8.1 / At3g54140 / PTR1 / CACCATGGAAGAAAAAGATGTGTATACGC / TCAATGTGCTCGACCAACAG
NPF8.2 / At5g01180 / PTR5 / CACCATGGAAGATGACAAGGATATATACACAAAAG / TCAAAGCGCATGCCCGG
NPF8.3 / At2g02040 / PTR2 / CACCATGGGTTCCATCGAAGAAGAAG / CTACGACGAAGCTTTCTTTTGC
NPF8.4 / At2g02020 / PTR4 / aaaaagcaggctATGGCTTCCATTGATGAAGAAAG / agaaagctgggtCCAGAAGAAGAGATCAAACCTT
NPF8.5 / At1g62200 / PTR6 / aaaaagcaggctATGGTGAATTCGAATGAAGAAGA / agaaagctgggtTTACAAAGCCTTCTTCTTTGTGT

Underlined sequences are overhangs for introduction into pENTR/D-TOPO.

Nucleotides shown in lowercase lettersare part of attB1 (for forward primers) and attB2 (for reverse primers) recombination sequences.

Sequences highlighted by dotted underlines in the reverse primers are 3’ untranslated regions of cDNAs.

N.A.; not amplified in this study.

Table S3 Conditions for LC

Hormone / Solvent A / Solvent B / Gradient (solvent B composition)
ABA / 0.01 % acetic acid / Acetonitrile 0.05% acetic acid / 3-40% (vol/vol) over 7 min
GA1, GA3, GA4 / 0.01 % acetic acid / Acetonitrile 0.05% acetic acid / 3-40% (vol/vol) over 10 min
JA-ILe / 0.01 % acetic acid / Acetonitrile 0.05% acetic acid / 3-60% (vol/vol) over 8 min

Table S4 Parameters for mass-spectrometry

Target / Retention time on LC (min) / ESI / MS/MS transitions for quantification (m/z) / Collision energy (V) / Declustering potential / Ion spray voltaging (V) / Desolvation temperature (°C)
ABA / 5.1 / − / 263.1/219.1 / 15 / 90 / 3,500 / 600
d6-ABA / − / 269.1/225.2
GA1 / 4.5 / − / 347.2/273.1 / 40 / 90 / 4,000 / 600
d2- GA1 / − / 349.2/275.1
GA3 / 4.4 / − / 345.2/221.1 / 40 / 90 / 4,000 / 600
d2- GA3 / − / 347.2/223.1
GA4 / 9.1 / − / 331.2/257.1 / 30 / 90 / 4,000 / 600
d2- GA4 / − / 333.2/259.1
(-)-JA-Ile / 7.5 / − / 322.2/130.1 / 30 / 90 / 3,500 / 600
d2-(-)-JA-Ile / − / 328.2/136.1
(+)-JA-Ile / 7.3 / − / 322.2/130.1 / 30 / 90 / 3,500 / 600
d2-(+)-JA-Ile / − / 328.2/136.1

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