Table S1. Plasmids and Bacterial Strains Used in This Study

Table S1. Plasmids and bacterial strains used in this study.

Strain/Plasmid

/

Relevant genotype*

/

Reference

E. coli

EC1000 / F-, araD139 (ara ABC-leu)7679, galU, galK, lacX74, rspL, thi, repA of pWV01 in glgB, KmR / (10)

S. pneumoniae

D39 / Serotype 2 strain, cps2 / (11)
DJS14 / D39, ΔbgaA::(P32_luc, tetR) / (2)
DJS15 / D39, ori::(P32_luc, tetR) / (2)
DLA9 / D39, ΔhexA::eryR / This study
DLA16 / D39, ΔhexA::eryR, ΔbgaA::(PssbB_luc, tetR) / This study
DLA17 / D39, comCDE_luc-Ps_kan / (2)
DLA18 / D39, ΔhexA::eryR, comCDE_luc-PS_kan / This study
DLA20 / D39, ΔhexA::eryR, comCDE_luc-PS_kan, ΔparB::spc / This study
DLA26 / D39, ΔhexA::eryR, parS(-1.6)mut / This study
DLA28 / D39, ΔhexA::eryR, comCDE_luc-PS_kan, parS(-1.6)mut / This study
DLA30 / D39, ΔhexA::eryR, ΔbgaA::(PssbB_luc, tetR), parS(-1.6)mut / This study
DLA37 / D39, ΔhexA::eryR, ΔbgaA::(PssbB_luc_gfp, tetR) / This study
DLA42 / D39, ΔhexA::eryR, parB-gfp_spc / This study
DLA43 / D39, ΔhexA::eryR, parB-gfp_spc, parS(-1.6)mut / This study
DLA53 / D39, ΔhexA::eryR, parS(-1.6)mut, complementation (parS(-1.6)WT, NotI) / This study
DLA54 / D39, ΔhexA::eryR, parS(-1.6)mut, no complementation (NotI) / This study
DLA59 / D39, ΔhexA::eryR, ΔbgaA::(PssbB_luc, tetR), parS(-1.6)mut, complementation (parS(-1.6)WT, NotI) / This study
DLA60 / D39, ΔhexA::eryR, ΔbgaA::(PssbB_luc, tetR), parS(-1.6)mut, no complementation (NotI) / This study
DLA65 / D39, ΔhexA::eryR, comCDE_luc-PS_kan, parS(-1.6)mut, complementation (parS(-1.6)WT, NotI) / This study
DLA66 / D39, ΔhexA::eryR, comCDE_luc-PS_kan, parS(-1.6)mut, no complementation (NotI) / This study
DLA72 / D39, ΔhexA::eryR, ΔbgaA::(PssbB_luc_gfp, tetR), ΔparB::spc / This study
DLA77 / D39, ΔhexA::eryR, parB-gfp_spc, parS(-1.6)mut, complementation (parS(-1.6)WT, NotI) / This study
DLA80 / D39, ΔhexA::eryR, parB-gfp_spc, parS(-1.6)mut, no complementation (NotI) / This study
DLA82 / D39, ΔhexA::eryR, comCDE_luc-PS_kan, ΔbgaA::(PssbB_mKate2, tetR) / This study
DLA84 / D39, ∆hexA::eryR, comCDE_luc-PS_kan, ΔbgaA::(PssbB_mKate2, tetR), ΔparB::spc / This study
DLA97 / D39, ΔhexA::eryR, ori::(P32_luc, tetR), parS(-1.6)mut, complementation (parS(-1.6)WT, NotI) / This study
DLA98 / D39, ΔhexA::eryR, ori::(P32_luc, tetR), parS(-1.6)mut, no complementation (NotI) / This study
DLA100 / D39, ΔhexA::eryR, ori::(P32_luc, tetR) / This study
DLA101 / D39, ΔhexA::eryR, ori::(P32_luc, tetR), ΔparB::spc / This study
DLA106 / D39, ΔbgaA::(parS(-1.6)WT, P32_luc, tetR) / This study
DLA107 / D39, ΔbgaA::(parS(-1.6)mut, P32_luc, tetR) / This study
MT2 / D39, parB-gfp_spc / (7)
MT3 / D39, ΔparB::spc / (7)
Plasmids
pKOE / pBluescript II KS+ derivative carrying a P32_ery_ter construct, ampR / Laboratory collection
pLA13 / ampR, bgaA’, tetR, PssbB_luc, ’bgaA / (2)
pLA18 / ampR, bgaA’, tetR, PssbB_luc_gfp, ’bgaA / (12)
pLA19 / ampR, bgaA’, tetR, P32_luc, ’bgaA / (2)
pLA21 / ampR, bgaA’, tetR, PssbB_mKate, ’bgaA / This study
pUC57-mKate / ampR, carries a synthetic fragment (GenScript) encoding the mKate2 fluorescent protein / (6)
pUC57-parS-1.6mut / ampR, carries a 670 bp synthetic fragment (GenScript) centered on a mutated version of the parS(-1.6) sequence / This study

Notations used in this table:

For antibiotic resistance, xxx stands for the coding sequence and xxxR stands for the gene with promoter and terminator.

For translational fusion, a dash ( - ) is used, e.g. parB-gfp.

For transcriptional fusion, an underscore ( _ ) is used, e.g. ssbB_luc.

In DLA97, DLA98, DLA100 and DLA101, the insertion (“ori::”) is at 29kb on the right arm of the chromosome, in an intergenic region between prsA and spd0034.

The abbreviations used are: amp for ampicillin, ery for erythromycin, kan for kanamycin, spc for spectinomycin, tet for tetracycline.