Table S1: Materials List

Materials
  1. Introduce the mystery: Case of the missing strawberries

  • Presentation S1 (display PowerPoint for class)

  1. Class discussion about DNA (Concept map and short-answer questions).

  • Presentation S1 (display PowerPoint for class)
  • DNA physical model and/or pictures of DNA
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  • Copies of worksheet 1 (Text Document S1)
  • Paper/white board for making concept map

  1. Class discussion- Introduction of the process of science

  • Presentation S1 (display PowerPoint for class)

  1. Lab activity: Isolate DNA from strawberries (quantities listed are for 6 student groups)

  • Copies of Isolation of strawberry DNA protocol ( 1 copy per student)
  • 1 Lab coat, 1 pair goggles, and 1 pair gloves per student
  • Strawberries- fresh or frozen (1-3 per group of students)
  • 5 ml Dawn dishsoap
  • 6 Quart size Ziploc bags
  • 1 Roll paper towels (give each student 1 paper towel)
  • 6 Funnels (at least 8 cm diameter, needs to fit inside 15 ml screw-cap tube)
  • 1 Glassbottle per class, 100-250 ml (for preparing DNA extraction buffer)
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  • 36 ml Ice-cold Isopropyl alcohol (91%)- (6 plastic screw-cap tubes each containing 6 ml of Isopropyl alcohol)
  • 1.5 g Sodium chloride (NaCl)
  • 6Graduated cylinders for measuring (100 ml)
  • 95 ml Tap water
  • 6 Graduated test tube or falcon tubes (15-mlclean, clear screw cap tubes or equivalent, for collecting liquid from strawberries)
  • 6 Holders for 15 ml screw-cap tubes
  • 6 Coffee stirrers (1 per student group)
  • 1 Light Microscope (for instructor set-up and students to view)
  • 6 Microscope slides and cover slips

  1. Class discussion of main concepts

  • Presentation S1 (display PowerPoint for class)
  • Document camera or white board (to display slides, display student concept maps, and write class notes during discussion)
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  • Physical model of DNA (optional)

  1. Lab activity: DNA digests (quantities listed are for 6 student groups)

  • Copies of restriction digest protocol (Text Document S2, 1 per student)
  • 1 Lab coat, 1 pair goggles, and 1 pair gloves per student
  • 6 P20 Micropipettors (1per student group)
  • 6 Boxes of Yellow tips for P20 micropipettor (1per student group)
  • 6 Microtubes(1 per student group) with colored water for practice pipetting (each tube contains 1 ml distilled water to which a few drops of red food coloring have been added)
  • 6 Pieces of saran wrap or parafilm (cut to 4 inch squares; 1 per student group)
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  • 6 Microtube racks (1 per student group)
  • Microfuge for quick spins (1 per class)
  • 10 µl each DNA from BioRad DNA fingerprinting kit (CS, S1, S2, S3, S4, S5), aliquoted into separate, labeledmicrotubes
  • EcoRI, PstI restriction enzyme mix from BioRad DNA fingerprinting kit, aliquot 10 µl each into each of 6 microtubes (label tubes “ENZ” for enzyme)
  • 6 Sharpie markers (1 per student group)
  • Ice bucket with ice (1 per class)
  • 370C Temperature block or water bath (1 per class)
  • 6 Waste containers for pipet tips (1 per student group)

  1. Hands-on activity: RFLP simulation (quantities listed are for 6 student groups)

  • 1 Copy of Figure S2, page 2 printed and cut into 6 strips(each strip has one of six 100 basepair double-stranded DNA sequences, 1 strip per student group)
  • 6 Scissors (1 per student group)
  • 1 roll scotch tape
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  • Drawing of gel on large paper or classroom white board (see Figures S3 and S4)
  • 13 pieces of colored paper cut into rectangles to represent DNA bands on the gel; each rectangle should be 1 inch X 11 inches

  1. Lab activity: Agarose gel electrophoresis (quantities listed are for 6 student groups)

  • Gel electrophoresis protocol (Text Document S3, 1 per student)
  • 1 Lab coat, 1 pair goggles, and 1 pair gloves per student
  • 500 ml Beaker (1 per class, for measuring tap water)
  • 250 ml Flask containing prepared 1% agarose (1 per class)
  • 1 Microwave
  • 1 Hot hand or hot pad holder (for holding hot flask)
  • 100 ml 100X Fast Blue Stain in 100-250 ml glass bottle, room temp
  • 1 Small (8 cm X 12 cm) plastic box with lid for staining gel (set on paper towels to keep spills off counter)
  • 1 Funnel (8 cm diameter) for returning stain to bottle
  • 3 Large (12 cm X 12 cm) plastic containers with 500ml warm tap water each for destaining gel
  • 30 l 6X gel loading dye (30% glycerol; 0.25% bromophenol blue)- (6microtubeseach containing 30 l dye; 1 per student group)
  • DNA molecular weight marker such as Lambda-HindIII (the marker should have a range of DNA fragments spanning the sizes of DNA fragments expected on the gel)
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  • 1 Spatula/pancake turner for moving gel
  • 1 Large (at least 18 cm X 18 cm) piece of saran wrap (to set destained gel on
  • 1 Piece of White paper (background for countertop when viewing gel)
  • 1 Mini-sized electrophoresis chamber- chamber, lid, gel tray, 8-well comb, 2 buffer dams
  • Compact power supply (capable of connecting to gel electrophoresis apparatus and running at constant 60 Volts)
  • 500 ml 1X TAE buffer (for running gel)
  • 6 P20 micropippetors and yellow tips (for loading gel, 1 set per student group)
  • 6 Boxes of yellow tips for P20 micropippetors (1 per student group)
  • Plastic rulers (1 per student group, rulers can be used to measure distance between gel bands and wells, if desired)

  1. Review

  • No materials needed

  1. Student elaboration

  • Computer(s) to view web resources in Table 2
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  • White board (for class discussion)

If teaching the lesson without the lab, include steps 1,2,3,5,7,9,10.