TABLE S1 - List of the Sequences Identified by Suppression Subtractive Hybridization Method

SUPPLEMENTARY MATERIAL

TABLE S1 - List of the sequences identified by Suppression Subtractive Hybridization method.

* In bold are the sequences characterized in this study.

Gene / Putative functions / Induced / Repressed library / Size (bp) / GenBank ID / Best Homology (species) / Score (bits) / E-value / Identities (aa/aa)
cyplp089 / ABA-responsive LEA-like protein / I / 356 / FJ379971 / Picea glauca / 99 (245) / 1.00E-19 / 50/57 (87%)
cyplp148 / Abscisic acid water deficit stress protein / I / 347 / FJ380030 / Pseudotsuga menziesii / 172 (436) / 8.00E-42 / 86/113 (76%)
cyplp126 / ACC oxidase / R / 437 / FJ380008 / Pseudotsuga menziesii / 192 (488) / 7.00E-48 / 118/153 (77%)
cyplp095 / Acyl carrier protein / I / 327 / FJ379977 / Cuphea lanceolata / 140 (353) / 3.00E-32 / 76/85 (89%)
cyplp145 / Adenosine kinase / I / 432 / FJ380027 / Nicotiana tabacum / 203 (517) / 3.00E-51 / 116/150 (77%)
cyplp150 / Alanine: glyoxylate aminotransferase / R / 478 / FJ380032 / Arabidopsis thaliana / 197 (501) / 2.00E-49 / 133/173 (76%)
cyplp014 / Aminocyclopropane-carboxylate oxidase / R / 466 / EU430716 / Rumex palustris / 89 (219) / 1.00E-22 / 44/91 (48%)
cyplp006 / Aquaporin / I / 364 / EU430708 / Picea mariana / 229 (584) / 5.00E-59 / 110/120 (91%)
cyplp129 / Aquaporin / I / 534 / FJ380011 / Samanea saman / 199 (507) / 6.00E-50 / 142/206 (68%)
cyplp104 / Aquaporin / I / 429 / FJ379986 / Taxodium distichum / 173 (438) / 5.00E-42 / 95/142 (66%)
cyplp008 / Beta-amylase / I / 550 / EU430710 / Arabidopsis thaliana / 72.4 (176) / 2.00E-11 / 42/92 (45%)
cyplp105 / Beta-amylase / I / 406 / FJ379987 / Glycine max / 223 (569) / 3.00E-57 / 119/137 (86%)
cyplp141 / Calcium-dependent protein kinase / R / 473 / FJ380023 / Pinus taeda / 123 (308) / 5.00E-27 / 79/120 (65%)
cyplp060 / Carbonate dehydratase protein / I / 355 / FJ237469 / Arabidopsis thaliana / 112 (279) / 1.00E-23 / 50/104 (48%)
cyplp044 / Carbonic anhydrase / I / 234 / FJ237453 / Flaveria pringlei / 111 (278) / 2.00E-23 / 51/77 (66%)
cyplp123 / Carbonic anhydrase / I / 423 / FJ380005 / Prunus dulcis / 240 (612) / 3.00E-62 / 121/140 (86%)
cyplp050 / Carboxyl-terminal peptidase protein / R / 457 / FJ237459 / Oryza sativa / 286 (733) / 3.00E-76 / 129/150 (86%)
cyplp061 / Carboxyl-terminal proteinase / R / 325 / FJ237470 / Oryza sativa / 194 (493) / 2.00E-48 / 87/99 (87%)
cyplp096 / CBF-like protein / I / 347 / FJ379978 / Camellia sinensis / 132 (332) / 9.00E-30 / 97/116 (83%)
cyplp054 / CBS domain protein / I / 453 / FJ237463 / Arabidopsis thaliana / 83.6 (205) / 5.00E-15 / 40/47 (85%)
cyplp122 / CBS domain-containing protein / I / 415 / FJ380004 / Solenostemon scutellarioides / 196 (497) / 6.00E-49 / 119/145 (82%)
cyplp004 / Chaperonin family protein / I / 382 / EU430706 / Oryza sativa / 208 (529) / 1.00E-52 / 102/127 (80%)
cyplp022 / Chlorophyll a/b-binding protein / I / 246 / EU430724 / Ophiopogon japonicus / 54.7 (130) / 2.00E-06 / 23/23 (100%)
cyplp154 / Chlorophyll a/b-binding protein / R / 389 / FJ380036 / Picea abies / 153 (386) / 5.00E-36 / 93/124 (75%)
cyplp080 / Chlorophyll a/b-binding protein / R / 262 / FJ237489 / Pinus pinaster / 174 (440) / 3.00E-42 / 82/85 (96%)
Gene / Putative functions / Induced / Repressed library / Size (bp) / GenBank ID / Best Homology (species) / Score (bits) / E-value / Identities (aa/aa)
cyplp005 / Chlorophyll a/b-binding protein / R / 402 / EU430707 / Pinus pinaster / 230 (587) / 2.00E-59 / 130/134 (97%)
cyplp135 / Chlorophyll a/b-binding protein / R / 510 / FJ380017 / Pinus roxburghii / 193 (491) / 4.00E-48 / 131/165 (79%)
cyplp062 / Chlorophyll a/b-binding protein / R / 372 / FJ237471 / Pinus thunbergii / 212 (539) / 9.00E-54 / 121/123 (98%)
cyplp064 / Chlorophyll a/b-binding protein / I / 318 / FJ237473 / Pseudotsuga menziesii / 112 (279) / 1.00E-23 / 80/109 (73%)
cyplp094 / Chloroplast fatty acid desaturase / I / 249 / FJ379976 / Brassica napus / 107 (266) / 4.00E-22 / 55/64 (85%)
cyplp015 / Dehydrin / I / 416 / EU430717 / Spinacia oleracea / 70.5 (171) / 4.00E-11 / 41/119 (34%)
cyplp102 / Cold acclimation protein / I / 378 / FJ379984 / Picea abies / 138 (348) / 1.00E-31 / 83/105 (79%)
cyplp110 / Cold acclimation protein / I / 439 / FJ379992 / Picea abies / 205 (522) / 8.00E-52 / 114/146 (78%)
cyplp125 / Cold induced protein / I / 346 / FJ380007 / Brassica napus / 132 (332) / 9.00E-30 / 81/117 (69%)
cyplp134 / Cold-inducible protein / I / 497 / FJ380016 / Camellia sinensis / 209 (531) / 8.00E-53 / 134/172 (77%)
cyplp099 / Constans-like protein / R / 448 / FJ379981 / Picea abies / 142 (358) / 9.00E-33 / 125/152 (82%)
cyplp081 / Cytochrome P450 / R / 263 / FJ237490 / Arabidopsis thaliana / 74.7 (182) / 2.00E-12 / 32/56 (57%)
cyplp041 / Dehydrin / I / 395 / * FJ237450 / Citrus sinensis / 68.6 (166) / 2.00E-10 / 43/111 (38%)
cyplp048 / Dehydrin / I / 311 / * FJ237457 / Coffea canephora / 119 (297) / 1.00E-25 / 81/104 (77%)
cyplp071 / Dehydrin / I / 512 / * FJ237480 / Panax ginseng / 163 (413) / 4.00E-39 / 123/181 (67%)
cyplp093 / Dehydrin / I / 308 / * FJ379975 / Picea abies / 94.7 (234) / 2.00E-18 / 78/104 (75%)
cyplp086 / Dehydrin / I / 357 / * FJ379968 / Picea abies / 118 (296) / 1.00E-25 / 75/95 (78%)
cyplp116 / Dehydrin / I / 395 / * FJ379998 / Pinus densata / 136 (343) / 5.00E-31 / 76/104 (73%)
cyplp114 / Dehydrin / I / 452 / * FJ379996 / Pinus sylvestris / 127 (320) / 2.00E-28 / 65/74 (87%)
cyplp138 / Dehydrin / I / 439 / * FJ380020 / Populus alba / 97.4 (241) / 3.00E-19 / 98/116 (84%)
cyplp133 / Dehydrin / I / 376 / * FJ380015 / Populus tremula / 105 (261) / 2.00E-21 / 88/122 (72%)
cyplp091 / Dehydrin / I / 371 / * FJ379973 / Rhododendron catawbiense / 107 (268) / 2.00E-22 / 94/125 (75%)
cyplp109 / Early drought induced protein / I / 299 / FJ379991 / Zea mays / 55.1 (131) / 2.00E-06 / 44/54 (81%)
cyplp077 / Early light-induced protein / I / 451 / * FJ237486 / Retama raetam / 130 (328) / 3.00E-29 / 85/113 (75%)
cyplp074 / Early light-induced protein / I / 391 / * FJ237483 / Rhododendron catawbiense / 114 (284) / 3.00E-24 / 54/101 (53%)
cyplp026 / Early light-induced protein / I / 436 / * FJ237435 / Rhododendron catawbiense / 157 (396) / 3.00E-37 / 72/110 (65%)
cyplp020 / Early light-induced protein / I / 443 / * EU430722 / Rhododendron catawbiense / 157 (398) / 2.00E-37 / 72/110 (65%)
cyplp132 / Early light-induced protein / I / 475 / * FJ380014 / Rhododendron catawbiense / 145 (366) / 1.00E-33 / 113/156 (72%)
cyplp088 / Early light-induced protein / I / 332 / * FJ379970 / Trifolium pratense / 98.2 (243) / 2.00E-19 / 78/95 (82%)
cyplp002 / Elongation factor 1A / R / 437 / EU430704 / Avicennia marina / 135 (339) / 1.00E-30 / 66/67 (98%)
cyplp013 / Exonuclease family protein / I / 481 / EU430715 / Oryza sativa / 98.2 (243) / 2.00E-19 / 46/99 (46%)
cyplp040 / F-box family protein / R / 458 / FJ237449 / Arabidopsis thaliana / 175 (443) / 1.00E-42 / 120/170 (70%)
cyplp121 / Flavanone hydroxylase / I / 428 / FJ380003 / Arabidopsis thaliana / 196 (499) / 4.00E-49 / 105/144 (72%)
cyplp058 / Fructose diphosphate aldolase / I / 481 / FJ237467 / Metasequoia glyptostroboides / 180 (456) / 4.00E-44 / 131/172 (76%)
Gene / Putative functions / Induced / Repressed library / Size (bp) / GenBank ID / Best Homology (species) / Score (bits) / E-value / Identities (aa/aa)
cyplp097 / Glutathione S-transferase / I / 338 / FJ379979 / Malva pusilla / 90.5 (223) / 4.00E-17 / 37/67 (55%)
cyplp037 / Glyceraldehyde phosphate dehydrogenase / I / 539 / FJ237446 / Atriplex nummularia / 189 (481) / 6.00E-47 / 156/217 (71%)
cyplp130 / Glyceraldehyde phosphate dehydrogenase / I / 459 / FJ380012 / Metasequoia glyptostroboides / 119 (299) / 6.00E-26 / 73/108 (67%)
cyplp066 / Glycolate oxidase / I / 326 / FJ237475 / Arabidopsis thaliana / 135 (341) / 8.00E-31 / 69/86 (80%)
cyplp103 / Heat shock protein / R / 356 / FJ379985 / Picea glauca / 179 (453) / 8.00E-44 / 92/105 (87%)
cyplp001 / Heat shock protein / R / 444 / EU430703 / Picea glauca / 173 (439) / 5.00E-45 / 85/115 (73%)
cyplp007 / Heavy-metal-binding protein / I / 229 / EU430709 / Oryza sativa / 99.4 (246) / 8.00E-20 / 48/75 (64%)
cyplp036 / Heavy-metal-binding protein / I / 229 / FJ237445 / Oryza sativa / 97.4 (241) / 3.00E-19 / 46/75 (61%)
cyplp052 / Hydrophobic LEA-like protein / I / 402 / FJ237461 / Pinus pinaster / 97.4 (241) / 3.00E-19 / 52/79 (65%)
cyplp018 / Hydrophobic LEA-like protein / I / 425 / EU430720 / Pinus pinaster / 104 (259) / 3.00E-21 / 52/79 (65%)
cyplp051 / Kinase protein / R / 439 / FJ237460 / Cathaya argyrophylla / 92 (227) / 1.00E-17 / 43/49 (87%)
cyplp117 / Late embryogenesis abundant protein / I / 409 / FJ379999 / Pseudotsuga menziesii / 85.9 (211) / 9.00E-16 / 43/46 (93%)
cyplp119 / Late embryogenesis abundant protein / I / 438 / FJ380001 / Tamarix androssowii / 152 (385) / 6.00E-36 / 86/103 (83%)
cyplp144 / Light-independent protochlorophyllide oxidoreductase / I / 477 / FJ380026 / Thuja plicata / 192 (488) / 7.00E-48 / 128/165 (77%)
cyplp025 / Light-independent protochlorophyllide oxidoreductase / I / 508 / FJ237434 / Metasequoia glyptostroboides / 235 (600) / 9.00E-61 / 149/195 (76%)
cyplp098 / Low temperature and salt responsive protein / I / 248 / FJ379980 / Solanum tuberosum / 90.1 (222) / 5.00E-17 / 42/50 (84%)
cyplp016 / Low temperature protein / I / 367 / EU430718 / Poncirus trifoliata / 78.6 (192) / 1.00E-13 / 43/54 (79%)
cyplp087 / Low temperature-induced protein / I / 372 / FJ379969 / Arabidopsis thaliana / 213 (541) / 5.00E-54 / 110/128 (85%)
cyplp076 / Low temperature-induced protein / I / 486 / FJ237485 / Arabidopsis thaliana / 163 (412) / 5.00E-39 / 122/177 (68%)
cyplp021 / Low-temperature-induced protein / I / 404 / EU430723 / Arabidopsis thaliana / 45.4 (106) / 1.00E-03 / 36/114 (31%)
cyplp010 / Lysine decarboxylase-like protein / I / 499 / EU430712 / Oryza sativa / 181 (458) / 2.00E-44 / 88/120 (73%)
cyplp155 / Malate dehydrogenase / I / 474 / FJ380037 / Pinus pinaster / 226 (576) / 4.00E-58 / 137/165 (83%)
cyplp152 / Metallothionein-like protein / I / 458 / FJ380034 / Elaeis guineensis / 112 (280) / 9.00E-24 / 57/65 (87%)
cyplp067 / Mitogen-activated protein kinase / I / 405 / FJ237476 / Picea abies / 191 (486) / 1.00E-47 / 101/126 (80%)
cyplp146 / NADH dehydrogenase / I / 486 / FJ380028 / Araucaria cunninghamii / 217 (553) / 2.00E-55 / 137/174 (78%)
cyplp032 / NADH-plastoquinone oxidoreductase / I / 471 / FJ237441 / Coffea arabica / 186 (472) / 5.00E-46 / 109/139 (78%)
cyplp065 / NADPH: protochlorophyllide oxidoreductase / R / 324 / FJ237474 / Lycopersicon esclulentum / 55.5 (132) / 1.00E-06 / 26/29 (89%)
cyplp068 / NADPH: protochlorophyllide oxidoreductase / R / 524 / FJ237477 / Pinus strobus / 239 (611) / 5.00E-62 / 152/190 (80%)
cyplp046 / Nodulin like-protein / I / 326 / FJ237455 / Pinus pinaster / 131 (330) / 2.00E-29 / 69/75 (92%)
cyplp003 / Nodulin-like protein / I / 430 / EU430705 / Pinus pinaster / 80.9 (198) / 3.00E-14 / 53/89 (59%)
cyplp072 / Oleosin / R / 389 / * FJ237481 / Camellia oleifera / 135 (341) / 8.00E-31 / 108/129 (83%)
cyplp139 / Oleosin / R / 376 / * FJ380021 / Corylus avellana / 137 (346) / 2.00E-31 / 114/128 (89%)
cyplp019 / Oleosin / R / 393 / * EU430721 / Lilium longiflorum / 92.4 (228) / 1.00E-17 / 51/88 (57%)
Gene / Putative functions / Induced / Repressed library / Size (bp) / GenBank ID / Best Homology (species) / Score (bits) / E-value / Identities (aa/aa)
cyplp034 / Oleosin / R / 393 / * FJ237443 / Lilium longiflorum / 92 (227) / 1.00E-17 / 51/88 (57%)
cyplp085 / Oleosin / R / 293 / * FJ237494 / Olea europaea / 113 (282) / 6.00E-24 / 76/101 (75%)
cyplp012 / Oxidoreductase family protein / R / 433 / EU430714 / Arabidopsis thaliana / 144 (363) / 2.00E-33 / 66/109 (60%)
cyplp111 / Pectin methylesterase protein / I / 436 / FJ379993 / Picea abies / 202 (515) / 5.00E-51 / 119/145 (82%)
cyplp142 / Photosystem I / R / 487 / FJ380024 / Cedrus libani / 176 (445) / 7.00E-43 / 126/184 (68%)
cyplp084 / Photosystem II cp47 protein / R / 372 / FJ237493 / Taxus brevifolia / 146 (369) / 5.00E-34 / 92/136 (67%)
cyplp082 / Plastocyanin, chloroplast precursor / R / 479 / FJ237491 / Populus nigra / 163 (412) / 5.00E-39 / 113/141 (80%)
cyplp137 / Pyruvate kinase / I / 502 / FJ380019 / Nicotiana tabacum / 247 (630) / 3.00E-64 / 144/177 (81%)
cyplp057 / Pyruvate kinase protein / I / 251 / FJ237466 / Oryza sativa / 64.7 (156) / 2.00E-09 / 29/35 (82%)
cyplp027 / QM-like protein / R / 312 / FJ237436 / Elaeis guineensis / 194 (492) / 2.00E-48 / 93/97 (95%)
cyplp075 / Rab1C protein / I / 393 / FJ237484 / Lotus japonicus / 241 (615) / 1.00E-62 / 118/131 (90%)
cyplp079 / Ribulose bisphosphate carboxylase / R / 396 / FJ237488 / Cephalocroton mollis / 190 (483) / 3.00E-47 / 107/138 (77%)
cyplp101 / Ribulose bisphosphate carboxylase / R / 407 / FJ379983 / Ophioglossum crotalophoroides / 203 (516) / 4.00E-51 / 112/140 (80%)
cyplp053 / Ribulose bisphosphate carboxylase / R / 459 / FJ237462 / Pinus engelmannii / 210 (534) / 3.00E-53 / 130/168 (77%)
cyplp124 / Ribulose bisphosphate carboxylase / R / 458 / FJ380006 / Wollemia nobilis / 198 (503) / 1.00E-49 / 121/151 (80%)
cyplp009 / Ribulose bisphosphate carboxylase activase / R / 438 / EU430711 / Gossypium hirsutum / 269 (688) / 5.00E-71 / 127/145 (87%)
cyplp024 / Senescence-associated protein / I / 392 / * EU430726 / Elaeis guineensis / 102 (253) / 1.00E-20 / 42/80 (52%)
cyplp045 / Senescence-associated protein / I / 237 / * FJ237454 / Elaeis guineensis] / 49.3 (116) / 1.00E-04 / 21/40 (52%)
cyplp023 / Senescence-associated protein / I / 294 / * EU430725 / Lilium longiflorum / 173 (439) / 3.00E-42 / 80/85 (94%)
cyplp073 / Senescence-associated protein / I / 422 / * FJ237482 / Oryza sativa / 142 (357) / 1.00E-32 / 65/121 (53%)
cyplp056 / Senescence-associated protein / I / 424 / * FJ237465 / Oryza sativa / 172 (437) / 6.00E-42 / 72/140 (51%)
cyplp031 / Senescence-associated protein / I / 475 / * FJ237440 / Oryza sativa / 192 (489) / 5.00E-48 / 80/157 (50%)
cyplp017 / Senescence-associated protein / I / 495 / * EU430719 / Oryza sativa / 198 (503) / 1.00E-49 / 83/158 (52%)
cyplp113 / Senescence-associated protein / I / 388 / * FJ379995 / Picea abies / 185 (469) / 1.00E-45 / 111/135 (82%)
cyplp070 / Senescence-associated protein / I / 393 / * FJ237479 / Picea abies / 198 (504) / 1.00E-49 / 103/131 (78%)
cyplp042 / Senescence-associated protein / I / 329 / * FJ237451 / Pisum sativum / 79 (193) / 1.00E-13 / 37/40 (92%)
cyplp011 / Serine/threonine kinase / I / 386 / EU430713 / Populus trichocarpa / 94.7 (234) / 2.00E-18 / 46/84 (54%)
cyplp107 / Serine/threonine protein kinase / I / 443 / FJ379989 / Lotus japonicus / 127 (320) / 2.00E-28 / 74/89 (83%)
cyplp100 / Subtilisin-like protein / I / 402 / FJ379982 / Picea abies / 207 (526) / 3.00E-52 / 124/139 (89%)
cyplp030 / Unknown / I / 224 / FJ237439 / - / - / - / -
cyplp153 / Unknown / I / 247 / FJ380035 / - / - / - / -
cyplp147 / Unknown / R / 251 / FJ380029 / - / - / - / -
cyplp047 / Unknown / I / 283 / FJ237456 / - / - / - / -
cyplp151 / Unknown / I / 283 / FJ380033 / - / - / - / -
Gene / Putative functions / Induced / Repressed library / Size (bp) / GenBank ID / Best Homology (species) / Score (bits) / E-value / Identities (aa/aa)
cyplp149 / Unknown / I / 304 / FJ380031 / - / - / - / -
cyplp063 / Unknown / R / 316 / FJ237472 / - / - / - / -
cyplp059 / Unknown / I / 334 / FJ237468 / - / - / - / -
cyplp055 / Unknown / I / 339 / FJ237464 / - / - / - / -
cyplp136 / Unknown / I / 341 / FJ380018 / - / - / - / -
cyplp033 / Unknown / R / 346 / FJ237442 / - / - / - / -
cyplp143 / Unknown / R / 364 / FJ380025 / - / - / - / -
cyplp118 / Unknown / I / 376 / FJ380000 / - / - / - / -
cyplp083 / Unknown / I / 382 / FJ237492 / - / - / - / -
cyplp038 / Unknown / R / 385 / FJ237447 / - / - / - / -
cyplp156 / Unknown / I / 389 / FJ380038 / - / - / - / -
cyplp028 / Unknown / I / 390 / FJ237437 / - / - / - / -
cyplp069 / Unknown / R / 393 / FJ237478 / - / - / - / -
cyplp092 / Unknown / I / 393 / FJ379974 / - / - / - / -
cyplp131 / Unknown / R / 413 / FJ380013 / - / - / - / -
cyplp140 / Unknown / I / 442 / FJ380022 / - / - / - / -
cyplp035 / Unknown / I / 469 / FJ237444 / - / - / - / -
cyplp112 / Unknown / R / 476 / FJ379994 / - / - / - / -
cyplp078 / Unknown / I / 486 / FJ237487 / - / - / - / -
cyplp090 / Unknown / I / 255 / FJ379972 / Picea sitchensis / 82 (201) / 1.00E-14 / 39/48 (81%)
cyplp128 / Unknown / I / 369 / FJ380010 / Picea sitchensis / 177 (449) / 2.00E-43 / 104/127 (81%)
cyplp108 / Unknown / R / 383 / FJ379990 / Picea sitchensis / 89.4 (220) / 9.00E-17 / 47/53 (88%)
cyplp049 / Unknown / I / 384 / FJ237458 / Picea sitchensis / 40.8 (94) / 4.00E-03 / 33/102 (32%)
cyplp043 / Unknown / I / 424 / FJ237452 / Picea sitchensis / 155 (392) / 1.00E-36 / 79/85 (92%)
cyplp106 / Unknown / I / 455 / FJ379988 / Picea sitchensis / 147 (371) / 3.00E-34 / 69/74 (93%)
cyplp127 / Unknown / I / 275 / FJ380009 / Populus trichocarpa / 95.5 (236) / 1.00E-18 / 56/68 (82%)
cyplp120 / Unknown / R / 436 / FJ380002 / Populus trichocarpa / 135 (339) / 1.00E-30 / 71/82 (86%)
cyplp029 / Water deficit inducible protein / I / 473 / FJ237438 / Pinus taeda / 185 (470) / 9.00E-46 / 115/137 (83%)
cyplp115 / Zinc finger family protein / I / 296 / FJ379997 / Picea abies / 91.7 (226) / 2.00E-17 / 64/77 (83%)
cyplp039 / Zinc finger protein-related / I / 493 / FJ237448 / Picea abies / 193 (491) / 3.00E-48 / 121/154 (78%)

TABLE S2 - Main features of amino acids multi-alignments used to build dendrograms

Alignment length (amino acids) / Conserved sites / Variable sites / Singleton sites
Oleosin / 135 / 2 / 133 / 3
SAG / 176 / 1 / 175 / 6
ELIP / 132 / 12 / 120 / 20
Dehydrin / 103 / 2 / 101 / 4

Figure S1 - Dendrogram based on the alignment of amino acid sequences from cypress putative dehydrin clones and sequences coded by Populus thrichocarpa, Vitis vinifera, Physcomitrella patens, Arabidopsis thaliana, and Pinus sylvestris. Bootstrap values based on 1000 replications are indicated on the branches. In bold are the sequences characterized in this study.