TABLES (S1 to S4)

Table S1 List of accessions used in the genetic diversity studies

S/No. / Accession No. / Origin / State/Country / S/No. / Accession No. / Origin / State/Country / S/No. / Accession No. / Origin / State/Country
1. / GE-4 / India / Uttar Pradesh / 28. / GE-1163 / India / Uttar Pradesh / 56. / GE-3019 / India / -
2. / GE-104 / India / Tamil Nadu / 29. / GE-1200 / India / Uttar Pradesh / 57. / GE-3138 / India / Karnataka
3. / GE-106 / India / Tamil Nadu / 30. / GE-1221 / India / Uttar Pradesh / 58 / GE-3147 / India / Karnataka
4. / GE-116 / India / Madya Pradesh / 31. / GE-1235 / India / Uttar Pradesh / 59. / GE-3149 / India / Karnataka
5. / GE-123 / India / Uttar Pradesh / 32. / GE-1240 / India / Tamil Nadu / 60. / GE-3219 / India / Andhra Pradesh
6. / GE-125 / India / Tamil Nadu / 33. / GE-1267 / India / Uttar Pradesh / 61. / GE-3235 / India / Andhra Pradesh
7. / GE-128 / India / - / 34. / GE-1280 / India / Tamil Nadu / 62. / GE-3248 / India / Andhra Pradesh
8. / GE-149 / India / - / 35. / GE-1298 / India / Uttar Pradesh / 63. / GE-3321 / India / Andhra Pradesh
9. / GE-218 / India / Karnataka / 36. / GE-1376 / India / Uttar Pradesh / 64. / GE-3764 / India / Maharashtra
10 / GE-224 / India / Uttar Pradesh / 37. / GE-1437 / India / Uttar Pradesh / 65. / GE-3767 / India / Maharashtra
11. / GE-225 / India / Uttar Pradesh / 38. / GE-1486 / India / Karnataka / 66. / GE-3885 / India / Maharashtra
12. / GE-258 / India / Uttar Pradesh / 39. / GE-1537 / India / Uttar Pradesh / 67. / GE-4004 / India / Maharashtra
13. / GE-278 / India / Uttar Pradesh / 40. / GE-1538 / India / Karnataka / 68. / GE-4404 / India / Bihar
14. / GE-356 / India / Uttar Pradesh / 41 / GE-1583 / India / Uttar Pradesh / 69. / GE-4449 / India / Orissa
15. / GE-390 / India / Tamil Nadu / 42. / GE-1621 / India / Karnataka / 70. / GE-4471 / India / Orissa
16. / GE-469 / India / - / 43. / GE-1680 / India / Karnataka / 71. / GE-4547 / Japan / -
17. / GE-482 / India / Andhra Pradesh / 44. / GE-1789 / India / Tamil Nadu / 72. / GE-4596 / India / Karnataka
18. / GE-489 / India / - / 45. / GE-1871 / India / Uttar Pradesh / 73. / GE-4600 / India / Karnataka
19. / GE-554 / India / Uttar Pradesh / 46. / GE-1899 / India / Uttar Pradesh / 74. / GE-4601 / India / Karnataka
20. / GE-591 / India / Uttar Pradesh / 47. / GE-1936 / India / Uttar Pradesh / 75. / GE-4668 / Africa / Uganda
21. / GE-619 / India / Tamil Nadu / 48. / GE-2063 / India / Tamil Nadu / 77. / GE-4692 / Africa / Uganda
22. / GE-724 / India / Uttar Pradesh / 49. / GE-2136 / India / Tamil Nadu / 78. / GE-4711 / Africa / Uganda
23. / GE-778 / India / Karnataka / 51. / GE-2246 / India / Uttar Pradesh / 79. / GE-4732 / Africa / Kenya
24. / GE-909 / India / Uttar Pradesh / 52. / GE-2347 / India / Uttar Pradesh / 80. / GE-4798 / Africa / Kenya
25. / GE-1045 / India / Karnataka / 53. / GE-2447 / India / Uttar Pradesh / 81. / GE-4806 / Africa / Kenya
26. / GE-1064 / India / Uttar Pradesh / 54. / GE-2471 / India / Uttar Pradesh / 82 / GE-4811 / Africa / Malawi
27. / GE-1146 / India / Uttar Pradesh / 55. / GE-2624 / India / Uttar Pradesh / 83. / GE-5194 Africa - / Uganda

Table S2 Eleusine coracana: summary of genetic diversity study using RAPD

S/No / Name
of primer / Primer sequence
(5’ to 3’) / Range of markers(kb) / Polymorphic bands/total
no. of bands / % Polymo-rphism / PIC / Region-specific bands (kb)
1 / RAPD-010 / CCA CAC TAC C / 0.15 -2.0 / 5/7 / 71.4 / 0.235 / Karnataka 0.2 kb
2 / RAPD-012 / CGG CCA CTG T / 0.30- 3.0 / 9/11 / 81.81 / 0.282 / Africa 0.5 kb
3 / RAPD-015 / CGG CCC CGG C / 0.40 -2.7 / 5/9 / 55.55 / 0.472 / Maharashtra 0.6 kb
4 / RAPD-04 / CGG AGA GCG A / 0.35- 1.3 / 2/3 / 66.66 / 0.184 / ---
5 / RAPD-09 / GAC GGA GCA G / 0.40- 3.0 / 3/6 / 50.0 / 0.203 / Uttar Pradesh 0.75 kb
6 / RAPD-036 / GAA GAA CCG C / 0.35-1.5 / 2/8 / 25.0 / 0.271 / Tamil Nadu 0.45 kb
7 / RAPD-021 / GAC GGA TCA G / 0.30- 1.8 / 4/7 / 57.14 / 0.139 / ---
8 / RAPD-011 / CGG AGA GCC C / 0.15- 3.5 / 6/11 / 54.54 / 0.487 / Africa 1.2 kb
9 / RAPD-031 / GGG TAA CGC C / 0.40 -3.0 / 5/8 / 62.5 / 0.358 / ---
10 / RAPD-030 / GGA CTG GAG T / 0.35- 2.7 / 11/15 / 73.33 / 0.260 / Uttar Pradesh 1.1 kb
11 / RAPD-14 / CGG CCC CGG T / 0.70- 1.5 / 1/5 / 20.0 / 0.180 / ---
T0TAL / 53/90 / 617.93
AVERAGE / 4.81/8.18 / 56.17% / 0.280

Table S3 Eleusine coracana: summary of genetic diversity study through SSR

S/No / Name
of primer / Primer sequence
(5’ to 3’) / Range of markers(kb) / Polymorphic bands/total
no. of bands / % Polymo-rphism / PIC / Region-specific bands (kb)
1 / SSR-01 / (F) GCGAAAACACAATGCAAAAA
(R) GCGTTGGTTGGACCTGAC / 0.15-4.0 / 8/12 / 66.66 / 0.885 / Africa 0.4 kb
2 / SSR-02 / (F) TCCTCCCTCCCTTCGCCCACTG
(R)CGATGTTCGCCATGGCAGCGACC / 0.2-3.0 / 10/15 / 66.66 / 0.872 / Tamil Nadu 0.3kb
3 / SSR-06 / (F) GCCTCGAGCATCATCATCAG
(R) ATCAACCTGCACTTGCCTGG / 0.7-4.2 / 7/10 / 70.0 / 0.910 / Africa 0.9 kb
4 / SSR-08 / (F) TTCCCTGTTAAGAGAGAAATC
(R) GTGTATTTGGTGAAAGCAAC / 0.4-1.2 / 11/15 / 73.0 / 0.903 / Uttar Pradesh 0.55 kb
5 / SSR-10 / (F) CTTTGTCTATCTCAAGACAC
(R) TTGCAGATGTTCTTCCTGATG / 0.35-1.9 / 15/18 / 83.33 / 0.922 / Karnataka 1.2 kb
T0TAL / 51/70 / 359.65
AVERAGE / 10.2/14 / 70.19% / 0.890

Table S4 Eleusine coracana: summary of genetic diversity study through cyt P450 gene based markers

S/No / Name of primer / Primer sequence
(5’ to 3’) / Range of markers(kb) / Polymorphic bands/total
no. of bands / % Polymo-rphism / PIC / Region-specific bands (kb)
1 / CYP2B6(F)
CYP2B6(R) / GACTCTTGCTACTCCTGGTT CGA ATACAG AGCTGATGAGT / 2.0-0.4.0 / 2/4 / 50.0 / 0.496 / ---
2 / CYP2C19(F)
Hem2C19(R) / TCCTTGTGCTCTGTCTCTCA TCCCACACAAATCCGTTTTCC / 3.0-0.3.5 / 6/11 / 54.54 / 0.132 / Tamil Nadu 0.6 kb
3 / Cyt 001 (F)
Cyt 002 (R) / GATGGTCTTCCGCGGTA CACTGGAAGGCGTGCA / 3.5-0.10 / 8/13 / 61.53 / 0.374 / Karnataka 1.2 kb
Uttar Pradeshn0.45 kb
4 / Cyt 003 (F)
Cyt 004 (R) / CGG CTTGCTCATGGA GAGAAATAGGTGGGTGGA / 3.0-0.40 / 4/13 / 30.76 / 0.148 / Africa 0.9 kb
5 / Cyt 005 (F)
Cyt 006 (R) / GACCCA AGC AACGTCA
GTGGGTTATGGCCCACA / 3.0-0.40 / 4/8 / 50.0 / 0.289 / Uttar Pradesh 0.5 kb
6 / Cyt 007 (F)
Cyt 008 (R) / GACGTGCCACTCTGCA
ACCCTAGGCTAAGGTGGA / 3.5-0.15 / 7/10 / 70.0 / 0.390 / --
7 / Cyt 009(F)
Cyt 010 (R) / CCACCT TGACGACCCAA
TGGCCCACATATTCACCA / 2.0-0.15 / 7/15 / 46.6 / 0.240 / Karnataka 0.55 kb
8 / Cyt 011 (F)
Cyt 012(R) / ACGTGCCACTCTGCAA
ACCCTAGGCTAAGGTGGA / 3.5-0.35 / 5/10 / 50.0 / 0.386 / Maharashtra 0.75 kb
9 / Cyt 013 (F)
Cyt 014(R) / GGGCCATAACCCACGA
ATTGGAGCGCCGGTGA / 3.5-0.10 / 6/11 / 54.54 / 0.318 / Africa 0.8 kb
10 / Cyt 015(F)
Cyt 016(R) / CCTGTACGACCCAAGCA
TGG CCCACA TATTCACCA / 2.5- 0.30 / 6/8 / 75.0 / 0.500 / Karnataka 1.0 kb Tamil
Nadu 0.9 kb
TOTAL / 10 primers / 55/103 / 542.97
AVERAGE / 5.5/10.3 / 54.29% / 0.327