Supplementary Material 1

Table S1. Data from proteins identified in TT- and IN-soluble extracts by LC-ESI-Q-TOF MS/MS.

Number / Protein / KOG / Sample / Organism / Access number or LophDB cluster ID / Peptides* / Mascot score / Coverage (%) / emPAI
Proteins identified only in TT samples
1 / Lactate dehydrogenase / C / TT / Opisthorchis viverrini / EL619924 / EVVESAYEIIR / 62 / 3 / -
2 / ADP/ATP carrier protein, mitochondrial / C / TT / Echinococcus granulosus / EGC00335 (contig1) / LLVQNQGEMLK / 54 / 6 / -
3 / Phosphoglucose isomerase / G / TT / Dugesia ryukyuensis / BW638082 / EFGIDEKNMFEFWDWVGGR / 77 / 8 / -
4 / Hypothetical transcription regulator / K / TT / Vibrio alginolyticus / ZP_01259795 / NIQSLER / 73 / 3 / -
5 / Na+/K+-ATPase alpha / P / TT / Taenia solium / Q6RWA9 / VAEIPFNSTNK; LPADKFPR; GVPVSQVNPR / 110 / 2 / -
6 / Actin related protein 2/3 complex, 20 kDa subunit / Z / TT / Dictyostelium discoideum / XP_646414 / HNKPEVEVKTSK / 68 / 5 / -
7 / Dynein light chain / Z / TT / Echinococcus granulosus / EGC01007 (BQ173410) / NFGSYVTHESK / 72 / 12 / -
8 / Paramyosin / Z / TT / Taenia solium / AAT94289 / FNADMAALK; LNAEVLR / 88 / 1 / -
Proteins identified only in IN samples
1 / ATP synthase beta subunit / C / IN / Ticodendron incognitum / AAN71673 / VAIVYGQMNEPPGAR / 68 / 3 / -
2 / Succinate dehydrogenase iron-sulfur protein / C / IN / Echinococcus granulosus / EGC00994 (BQ173388) / YLGPAVLQQAYR / 50 / 5 / -
3 / Succinate-CoA ligase alpha chain / C / IN / Caenorhabditis briggsae / Q616R1 / MGHAGAIIAGGK / 66 / 4 / -
4 / 40S ribosomal protein S18 / J / IN / Echinococcus granulosus / EGC00308 (contig1) or EGC03315 (contig1) / FQHILR / 28 / 7 / -
5 / 60S ribosomal protein L21 / J / IN / Aedes aegypti / XP_001652845 / HALGVIVNK / 66 / 6 / -
6 / Threonyl-tRNA synthetase / J / IN / Schistosoma japonicum / CV674537 or CV749216 / WPFWLSPR / 66 / 2 / -
7 / FK506 binding protein / O / IN / Aeromonas hydrophila / YP_855549 / VHYTGTLTDGTK / 74 / 4 / -
8 / Hsp20 / O / IN / Taenia solium / EL752175 or EL754279 / SIPLPPSVDR; AFVTPEVIDASK / 89 / 9 / -
9 / Hsp60 / O / IN / Fusarium graminearum / XP_386422 / LLQDVASKTNEVAGDGTTTATVLAR / 58 / 4 / -
10 / Hsp90 / O / IN / Echinococcus granulosus / EGC00627 (contig1) / HSQFISYPIK / 53 / 3 / -
11 / 26S proteasome regulatory complex, subunit RPN11 / O / IN / Echinococcus granulosus / EGC03094 (contig1) / LINPNMVIANQEPR / 45 / 6 / -
12 / Cysteine and glycine-rich protein 1 / TZ / IN / Taenia solium / EL741892, EL743667, EL749282, EL749615, EL752162, EL752262, EL752399, EL752599, EL752855, EL754165, EL755542, EL756153, EL757534, EL758587, EL759499, EL759601, EL762079, EL762307 or EL762799 / SLDSTNLATK; GYGFGQGAGVLNTEK / 79 / 13 / -
13 / Beta-tubulin / Z / IN / Echinococcus granulosus / EGC04893 (contig1) / GHYTEGAELVESVLDVIR / 29 / 9 / -
Proteins identifiedin TT and IN samples
1 / Malate dehydrogenase, cytoplasmic / C / TT / Echinococcus granulosus / EGC00028 (contig1) / VLVVGNPANTNCLIMSK; VLITGAAGQIAYNLSNMVANGNLFGK / 150 / 13 / 0.57
IN / Echinococcus granulosus / EGC00028 (contig1) / VLVVGNPANTNCLIMSK; VLITGAAGQIAYNLSNMVANGNLFGK / 217 / 13 / 0.57
2 / Phosphoenolpyruvate carboxykinase / C / TT / Echinococcus granulosus / 191 (our clustering)/ EGC04111 (cluster1) / NSVLTNVAETDDGR; AINPEAGFFGVAPGTNHK; TNMAMLTPSLPGWK; IAYDEGWMAEHMLIMSVTNPK; FCCPASQCPIIHPK; WEDPAGVPVSVFMFGGR / 420 / 23 / 0.77
IN / Echinococcus granulosus / 191 (our clustering)/ EGC04111 (cluster1) / NSVLTNVAETDDGR; AINPEAGFFGVAPGTNHK; TNMAMLTPSLPGWK; IAYDEGWMAEHMLIMSVTNPK; FCCPASQCPIIHPK; WEDPAGVPVSVFMFGGR / 529 / 23 / 0.77
3 / Phosphoenolpyruvate carboxykinase / C / TT / Echinococcus granulosus / 458 (our clustering) /EGC04068 (contig1) / HGVMIGAQVK; RPEGIPLVYEAR / 93 / 12 / 0.50
IN / Echinococcus granulosus / 458 (our clustering) /EGC04068 (contig1) / SEATAAAEFK; HGVMIGAQVK; RPEGIPLVYEAR / 145 / 17 / 1.24
4 / Pyrophosphatase / C / TT / Echinococcus granulosus / EGC00895 (BQ173221) / VLGILAMIDEGETDWK / 54 / 8 / 0.25
IN / Echinococcus granulosus / EGC00895 (BQ173221) / VLGILAMIDEGETDWK / 72 / 8 / 0.25
5 / Translationally-controlled tumor protein (TCTP) / DZ / TT / Echinococcus granulosus / EGC03178 (contig1) / DFLTGDELFSDAFPIK / 110 / 8 / 0.27
IN / Echinococcus granulosus / EGC03178 (contig1) / DFLTGDELFSDAFPIK / 75 / 8 / 0.27
6 / Ornithine aminotransferase / E / TT / Echinococcus granulosus / EGC00294 (BI244007) / VLPMNTGVEGGETALK / 82 / 13 / 0.44
IN / Echinococcus granulosus / EGC00294 (BI244007) / VLPMNTGVEGGETALK / 92 / 13 / 0.44
7 / 6-phosphogluconate dehydrogenase / G / TT / Mesocestoides corti / CX863956 / GCPITLIAESVFSR / 48 / 11 / 0.24
IN / Mesocestoides corti / CX863956 / GCPITLIAESVFSR / 74 / 11 / 0.24
8 / Enolase / G / TT / Echinococcus granulosus / EGC03002 (CN649593) / LAMQEFMILPTGAK; AAVPSGASTGVHEAVELR / 116 / 16 / 0.42
IN / Echinococcus granulosus / EGC03002 (CN649593) / LAMQEFMILPTGAK; AAVPSGASTGVHEAVELR / 154 / 16 / 0.42
9 / Enolase / G / TT / Echinococcus granulosus / EGC04828 (CN653186) / SGETEDSTIADIVVGLR; AGIQIVGDDLTVTNPER / 435 / 22 / 0.85
IN / Echinococcus granulosus / EGC04828 (CN653186) / SGETEDSTIADIVVGLR; AGIQIVGDDLTVTNPER / 314 / 22 / 0.85
10 / Fructose-bisphosphate aldolase / G / TT / Echinococcus granulosus / EGC00369 (contig1) / ALQASAIAAWQGKPENVK; ISSHNPSYLAMLENANVLAR; VDLGVVPLGGTADECTTQGLDNLAQR / 145 / 17 / 0.47
IN / Echinococcus granulosus / EGC00369 (contig1) / ALQASAIAAWQGKPENVK; ISSHNPSYLAMLENANVLAR; VDLGVVPLGGTADECTTQGLDNLAQR / 162 / 17 / 0.47
11 / Glycogen phosphorylase / G / TT / Taenia solium / EL741014, EL747076 or EL750163 / IFPAADLSQQISTAGTEASGTGNMK / 111 / 11 / 0.13
IN / Taenia solium / EL741014, EL747076 or EL750163 / IFPAADLSQQISTAGTEASGTGNMK / 162 / 11 / 0.13
12 / Glyceraldehyde-3-phosphate dehydrogenase / G / TT / Echinococcus granulosus / EGC00305 (contig1) / AGIALNDTFVK; VPTPNVSVVDLTCK / 613 / 16 / 0.50
IN / Echinococcus granulosus / EGC00305 (contig1) / AGIALNDTFVK; VPTPNVSVVDLTCK / 610 / 16 / 0.50
13 / putative Glycogenin / G / TT / Taenia solium / EL761034 / FSNIQAALDK / 74 / 14 / 0.58
IN / Taenia solium / EL761034 / FSNIQAALDK / 80 / 14 / 0.58
14 / Phosphoglycerate kinase / G / TT / Taenia solium / EL740229 / IQLINNMLDK; DPAPGTVFLLENLR / 95 / 12 / 0.18
IN / Taenia solium / EL740229 / IQLINNMLDK; DPAPGTVFLLENLR / 74 / 12 / 0.18
15 / Phosphoglycerate mutase / G / TT / Echinococcus granulosus / EGC03341 (contig1) / VLITAHGNSLR / 43 / 4 / 0.13
IN / Echinococcus granulosus / EGC03341 (contig1) / MYGGLQGLNK; HGESVYNQENR / 39 / 8 / 0.29
16 / GDP-mannose 4,6-dehydratase / G / TT / Taenia solium / EL757899 / VALITAVTGQDGSYLAELLLSK / 78 / 31 / 0.87
IN / Taenia solium / EL757899 / VALITAVTGQDGSYLAELLLSK / 60 / 31 / 0.87
17 / Elongation factor1 alpha / J / TT / Echinococcus granulosus / EGC00741 (contig1) / KLIIAVNK; FQEISSEMK; STSTGHLIYK; YLVTIIDAPGHR / 169 / 19 / 1.17
IN / Echinococcus granulosus / EGC00741 (contig1) / KLIIAVNK; FQEISSEMK; STSTGHLIYK; YLVTIIDAPGHR / 177 / 19 / 1.17
18 / Elongation factor 2 / J / TT / Echinococcus granulosus / 1158 (our clustering)/EGC04141 (contig1) / GIIFSEENTPGTPIYIVK / 31 / 10 / 0.30
IN / Echinococcus granulosus / 1158 (our clustering)/EGC04141 (contig1) / GIIFSEENTPGTPIYIVK / 31 / 10 / 0.30
19 / 40S ribosomal protein S4 / J / TT / Echinococcus granulosus / EGC00738 (contig1) / HPGSVEMVHVR / 43 / 4 / 0.12
IN / Echinococcus granulosus / EGC00738 (contig1) / HPGSVEMVHVR / 47 / 4 / 0.12
20 / 40S ribosomal protein S3 / J / TT / Echinococcus granulosus / EGC00289 (contig1) / LPQGILGIR / 43 / 3 / 0.14
IN / Echinococcus granulosus / EGC00289 (contig1) / LPQGILGIR / 45 / 3 / 0.14
21 / 60S ribosomal protein L15 / J / TT / Echinococcus granulosus / EGC00645 (contig1) or EGC02266 (CV680511) / GATYGKPTNEGVNQLK / 66 / 7 / 0.24
IN / Echinococcus granulosus / EGC00645 (contig1) or EGC02266 (CV680511) / GATYGKPTNEGVNQLK / 62 / 7 / 0.24
22 / tRNA pseudouridine synthetase B / J / TT / Mycobacterium leprae / NP_302076 / ILGLLAGASK / 59 / 3 / 0.11
IN / Mycobacterium leprae / NP_302076 / ILGLLAGASK / 57 / 3 / 0.11
23 / Histone H2A / B / TT / Echinococcus granulosus / EGC03425 (contig1) or EGC03590 (CN650779) / HLQLAIR; AGLQFPVGR / 76 / 11 / 0.27
IN / Echinococcus granulosus / EGC03425 (contig1) or EGC03590 (CN650779) / HLQLAIR; AGLQFPVGR / 69 / 11 / 0.27
24 / Histone H3 / B / TT / Echinococcus granulosus / EGC01939 (contig1) / STELLIR; YRPGTVALR / 42 / 11 / 0.56
IN / Echinococcus granulosus / EGC01939 (contig1) / STELLIR; YRPGTVALR / 93 / 11 / 0.56
25 / Histone H4 / B / TT / Echinococcus granulosus / EGC00600 (contig1) / DNIQGITKPAIR / 80 / 11 / 0.82
IN / Echinococcus granulosus / EGC00600 (contig1) / VFLENVIR; DAVTYTEHAK; DNIQGITKPAIR / 109 / 28 / 2.30
26 / 14-3-3 zeta / O / TT / Echinococcus granulosus / EGC02546 (contig1) / DSTLIMQLLR / 94 / 4 / 0.12
IN / Echinococcus granulosus / EGC02546 (contig1) / DSTLIMQLLR / 92 / 4 / 0.12
27 / 14-3-3 epsilon / O / TT / Echinococcus granulosus / EGC00123 (BF643012) / DATLIMQLLR / 93 / 4 / 0.14
IN / Echinococcus granulosus / EGC00123 (BF643012) / DATLIMQLLR / 58 / 4 / 0.14
28 / Cyclophilin / O / TT / Echinococcus granulosus / EGC00016 (contig1) / HSKPMMLSMANAGK / 160 / 8 / 0.48
IN / Echinococcus granulosus / EGC00016 (contig1) / HSKPMMLSMANAGK / 144 / 8 / 0.48
29 / Hsp70 / O / TT / Mesocestoides corti / AAB18390 / NQVAMNPSNTVFDAK; VSDAVVTVPAYFNDSQR; IINEPTAAAIAYGLDK / 145 / 7 / 0.16
IN / Mesocestoides corti / AAB18390 / WLDANQQADKEEYEHR; STAGDTHLGGEDFDSR; IINEPTAAAIAYGLDKK; KVSDAVVTVPAYFNDSQR; VSDAVVTVPAYFNDSQR; VINSGGKPMIEVEYR; NQVAMNPSNTVFDAK / 485 / 15 / 0.29
30 / Thioredoxin peroxidase / O / TT / Echinococcus granulosus / EGC00084 (contig1), EGC02722 (contig1) or EGC01022 (BQ173439) / QITINDLPVGR / 74 / 5 / 0.17
IN / Echinococcus granulosus / EGC00084 (contig1), EGC02722 (contig1) or EGC01022 (BQ173439) / QITINDLPVGR / 78 / 5 / 0.17
31 / Transitional endoplasmic reticulum ATPase (TER ATPase) - Valosin-containing protein (VCP) / O / TT / Schistosoma japonicum / Q5D9C5 / KYEMFAQTLQQSR / 70 / 1 / 0.06
IN / Schistosoma japonicum / Q5D9C5 / KYEMFAQTLQQSR / 93 / 1 / 0.06
32 / Ubiquitin / OR / TT / Echinococcus granulosus / EGC01137 (contig1), EGC00094 (contig1), EGC00094 (contig2), EGC00481 (contig1), EGC00481 (contig2), EGC02744 (CN649084), EGC02671 (contig1), EGC02773 (CN649145) or EGC02662 (CN648925) / TITLEVEPSDSIENVK / 88 / 11 / 0.39
IN / Echinococcus granulosus / EGC01137 (contig1), EGC00094 (contig1), EGC00094 (contig2), EGC00481 (contig1), EGC00481 (contig2), EGC02744 (CN649084), EGC02671 (contig1), EGC02773 (CN649145) or EGC02662 (CN648925) / TITLEVEPSDSIENVK / 83 / 11 / 0.39
33 / Aldo/keto redutase / R / TT / Echinococcus granulosus / EGC02895 (CN649349) / TPAQILIR / 63 / 6 / 0.26
IN / Echinococcus granulosus / EGC02895 (CN649349) / TPAQILIR / 56 / 6 / 0.26
34 / Ezrin/radixin/moesin-like protein (tegument protein or antigen II/3) / R / TT / Echinococcus multilocularis / DR748399, DR748417 or DR748734 / EQYDQTDEQWYER / 63 / 5 / 0.20
IN / Echinococcus multilocularis / DR748399, DR748417 or DR748734 / EQYDQTDEQWYER / 48 / 5 / 0.20
35 / Actin / Z / TT / Echinococcus granulosus / EGC00006 (contig1) / DLTDYLMK; GYSFTTTAER; HQGVMVGMGQK; VAPEEHPVLLTEAPLNPK; TTGIVLDSGDGVTHSVPIYEGYALPHAILR / 289 / 34 / 0.94
IN / Echinococcus granulosus / EGC00006 (contig1) / DLTDYLMK; GYSFTTTAER; HQGVMVGMGQK; VAPEEHPVLLTEAPLNPK; TTGIVLDSGDGVTHSVPIYEGYALPHAILR / 238 / 34 / 0.94
36 / Actin / Z / TT / Echinococcus granulosus / EGC00006 (contig2) / SYELPDGQVITIGNER; DLYANTVLSGGSTMYPGIADR; LCYVALDFEQEMATAASSSSLEK / 335 / 28 / 0.9
IN / Echinococcus granulosus / EGC00006 (contig2) / SYELPDGQVITIGNER; DLYANTVLSGGSTMYPGIADR; LCYVALDFEQEMATAASSSSLEK; CPEALYQPSFLGMEAVGIHETTFNSIMK / 427 / 41 / 1.91
37 / Actin / Z / TT / Echinococcus granulosus / EGC00007 (contig1) / GYAFTTTAER; QQSIMVGMGNK; VAPEEHPVLLTEAPLNPK; TTGIVLDSGDGVSHTVPIYEGYALPHAILR / 234 / 32 / 0.82
IN / Echinococcus granulosus / EGC00007 (contig1) / GYAFTTTAER; QQSIMVGMGNK; VAPEEHPVLLTEAPLNPK; TTGIVLDSGDGVSHTVPIYEGYALPHAILR / 195 / 32 / 0.82
38 / Actin-binding and severin family group-like protein / Z / TT / Taenia solium / EL762236 / SAAAAFLQDLESK / 94 / 8 / 0.20
IN / Taenia solium / EL762236 / SAAAAFLQDLESK / 86 / 8 / 0.20
39 / Myosin heavy chain / Z / TT / Schistosoma mansoni / CAA30555 / VKPMLNIAR; QQLSAQLEEAR / 88 / 1 / 0.02
IN / Schistosoma mansoni / CAA30555 / VKPMLNIAR; QQLSAQLEEAR / 85 / 1 / 0.02
40 / Dynein light chain / Z / TT / Taenia solium / EL748083 or EL758264 / NADMSEEMQQDAVAVAGQALEK / 164 / 22 / 0.57
IN / Taenia solium / EL748083 or EL758264 / NADMSEEMQQDAVAVAGQALEK / 157 / 22 / 0.57
41 / Dynein light chain / Z / TT / Echinococcus granulosus / EGC00490 (contig1) / FGSEIAYVK / 46 / 8 / 0.31
IN / Echinococcus granulosus / EGC00490 (contig1) / FGSEIAYVK / 57 / 8 / 0.31
42 / Myophilin / Z / TT / Echinococcus granulosus / EGC00027 (contig1) / IMENISAFLEAMK / 46 / 6 / 0.17
IN / Echinococcus granulosus / EGC00027 (contig1) / INENATMPFK; IMENISAFLEAMK / 134 / 12 / 0.37
43 / Alpha-tubulin / Z / TT / Echinococcus granulosus / EGC00234 (contig1) / FDGALNVDLTEFQTNLVPYPR / 47 / 4 / 0.1
IN / Echinococcus granulosus / EGC00234 (contig1) / AVFVDLEPTVVDEVR; VGINYQPPTVVPGGDLAK / 51 / 6 / 0.21
44 / Beta-tubulin / Z / TT / Echinococcus granulosus / EGC03115 (CN649797) / GHYTEGAELVDSVLDVIR / 62 / 10 / 0.33
IN / Echinococcus granulosus / EGC03115 (CN649797) / AGPFGQIFRPDNFVFGQSGAGNNWAK / 26 / 15 / 0.33
45 / Conserved hypothetical protein / - / TT / Echinococcus granulosus / EGC03456 (CN650496) / LQSALVNSEK / 59 / 4 / 0.15
IN / Echinococcus granulosus / EGC03456 (CN650496) / LQSALVNSEK / 32 / 4 / 0.15

* p< 0.05