Table III: List of Protein Spots and Their Contents Identified by MS/MS in Leiomyomas And

Table III: List of protein spots and their contents identified by MS/MS in leiomyomas and myometrium.

Protein Spots /

Description

/ Accession Number / Theoretical
MW / Theoretical
pI / Score / Match / Transcripts
*
76 / Annexin A1
Aflatoxin B1 Aldehyde reductase 2
Splice Isoform 1 Heterogeneous nuclear ribonucleoprotein H3
PDZ and LIM domain protein 1
PTD012 protein
LIM and SH3 domain protein 1 / P04083
O43488
P31942
O00151
Q6FI88
Q14847 / 38.6
39.6
36.9
35.9
35.1
29.7 / 6.64
6.70
6.37
6.55
6.23
6.61 / 361
359
327
257
217
114 / 10
11
9
5
7
3 / +
+
+
+
+
+
93 / CGI-150 protein
Inorganic pyrophosphatase / Q9Y3E8
Q9H2U2 / 55.0
38.0 / 8.89
7.07 / 495
106 / 13
3 / +
+
107 / Annexin V

Mimecan

Microtubule-associated protein RP/EB1
EF-hand domain-containing protein 2
Tubulin-specific chaperone B / P08758
P20774
Q15691
Q96C19
Q99426 / 35.8
33.9
29.9
26.7
27.3 / 4.94
5.46
5.02
5.15
5.06 / 619
329
180
155
132 / 23
6
4
3
3 / +
+
+
+
+
126 / 14-3-3
14-3-3
14-3-3
14-3-3
Annexin V
Proteasome 5
Vimentin
Acidic leucine-rich nuclear phosphoprotein 32A / Q04917
P27348
P61981
P31946
P08758
P28066
P08670
P39687 / 28.1
27.8
28.2
28.0
35.8
26.4
53.5
28.6 / 4.76
4.68
4.80
4.76
4.94
4.74
5.06
3.99 / 339
317
231
170
162
157
124
92 / 8
7
8
6
4
4
3
2 / +
+
+
+
+
+
+
+
183 /  crystallin B chain / P02511 / 20.2 / 6.76 / 489 / 28 / +
187 / Transgelin (SM22) / Q5U0D2 / 22.6 / 8.87 / 632 / 28 / +
192 / Myosin regulatory light chain
2, smooth muscle isoforms
Myosin regulatory light chain 2, nonsarcomeric / P24844
P19105 / 19.7
19.7 / 4.8
4.67 / 333
224 / 15
9 / +
+
204 / Splice Isoform CFL2b of Cofilin, muscle isoforms
Cofilin, non-muscle isoforms
Transgelin (SM22) / Q9Y281
P23528
Q01995 / 18.7
18.4
22.5 / 7.66
8.26
8.88 / 263
172
96 / 10
6
3 / +
+
+
+
206 / Transgelin (SM22)
Destrin alpha / Q01995 / 22.5 / 8.88 / 329
309 / 14
17 / +
+
209 / Actin-related protein 2/3 complex subunit 5 / O15511 / 16.2 / 5.47 / 245 / 14 / +
243 / Retinoic acid-binding protein II, cellular
14 kDa phosphohistidine phosphatase / P29373
Q9NRX4 / 15.6
13.8 / 5.43
5.65 / 208
105 / 19
3 / +
+
303 / Calgizzarin (S100A11)
Cytochrome c oxidase polypeptide Vib / P31949 / 11.7 / 6.56 / 164
64 / 10
4 / +
+
324 / Ubiquitin / P62988 / 8.56 / 6.56 / 203 / 17 / +

The identified proteins and their corresponding MASCOT scores obtained by 2D-PAGE separation combined with MALDI-TOFMS PMF and nano-HPLC-ESI-MS/MS peptide sequencing. Spot numbers corresponds to data in Fig. 3 with changes in their expression reported in Table II. The corresponding transcripts (*) of the proteins identified by microarray in leiomyomas and myometrium at p0.05 and 2-fold cutoff are shown as + in the right hand side column. The Accession numbers, theoretical MW and pI are according to and calculated from Swiss-Prot.