TABLE 2. Protein coding genes in the hypothetical minimal cell

GENE / E.C. NUMBER / Bacillus subtilis / Mycoplasma genitalium / Escherichia coli / Wigglesworthia glossinidia / Buchnera aphidicola BAp / Buchnera aphidicola BSg / Buchnera aphidicola BBp / Blochmannia floridanus / PROTEIN FUNCTION
1. INFORMATION STORAGE AND PROCESSING (116 genes)
1.1. DNA metabolism (16 genes)
1.1.1. Basic replication machinery (13 genes)
dnaB / 3.6.1.- / BG10004 / MG094 / b4052 / dnaB / BUsg528 / BU546 / BBp489 / Bfl027 / replicative DNA helicase
dnaE / 2.7.7.7 / BG12583 / MG261 / b0184 / dnaE / BUsg233 / BU238 / BBp220 / Bfl286 / DNA polymerase III,  subunit
dnaG / 2.7.7.- / BG10313 / MG250 / b3066 / dnaG / BUsg053 / BU056 / BBp053 / Bfl057 / DNA primase
dnaN / 2.7.7.7 / BG10066 / MG001 / b3701 / dnaN / BUsg011 / BU011 / BBp011 / Bfl016 / DNA polymerase III,  subunit
dnaQa / 2.7.7.7 / BG10263 / MG031 / b0215 / dnaQ / BUsg240 / BU248 / BBp230 / Bfl225 / DNA polymerase III,  subunit
dnaX / 2.7.7.7 / BG10083 / MG420 / b0470 / dnaX / BUsg466 / BU481 / BBp425 / Bfl301 / DNA polymerase III,  and  subunits
gyrA / 5.99.1.3 / BG10071 / MG004 / b2231 / gyrA / BUsg174 / BU180 / BBp169 / Bfl476 / DNA gyrase, A subunit
gyrB / 5.99.1.3 / BG10070 / MG003 / b3699 / gyrB / BUsg010 / BU010 / BBp010 / Bfl017 / DNA gyrase, B subunit
holAb / 2.7.7.7 / BG11642 / MG315 / b0640 / holA / BUsg430 / BU445 / BBp396 / Bfl311 / DNA polymerase III,  subunit
holB / 2.7.7.7 / BG10095 / MG007 / b1099 / holB / BUsg342 / BU354 / BBp324 / Bfl400 / DNA polymerase III, ' subunit
hupAc / BG10276 / MG353 / b4000 / hupA / BUsg033 / BU032 / BBp033 / Bfl280 / DNA-binding protein
lig / 6.5.1.2 / BG12835 / MG254 / b2411 / lig / BUsg064 / BU067 / BBp062 / Bfl507 / DNA ligase (NAD-dependent)
ssb / BG10048 / MG091 / b4059 / ssb / BUsg527 / BU545 / BBp488 / Bfl028 / single-strand DNA-binding protein
1.1.2. DNA repair, restriction, and modification (3 genes)
nthd / 4.2.99.18 / BG10956 / MG235 / b1633 / nth / BUsg111 / BU119 / BBp114 / Bfl372 / endonuclease III
polAe / 3.1.11.- / BG12656 / MG262 / b3863 / polA / BUsg416 / BU431 / BBp382 / Bfl619 / 5'-3' exonuclease domain of DNA polymerase I
ungf / 3.2.2.- / BG10603 / MG097 / b2580 / ung / BUsg177 / BU183 / BBp172 / Bfl543 / uracil-DNA glycosylase
1.2. RNA metabolism (106 genes)
1.2.1. Basic transcription machinery (8 genes)
deaD / BG11143 / MG425 / b3162 / deaD / BUsg360 / BU372 / BBp335 / Bfl109 / ATP-dependent RNA helicase
greA / BG19019 / MG282 / b3181 / greA / BUsg371 / BU384 / BBp347 / Bfl096 / transcription elongation factor
nusA / BG10265 / MG141 / b3169 / nusA / BUsg366 / BU378 / BBp341 / Bfl103 / transcription translation coupling
nusG / BG10162 / MG054 / b3982 / nusG / BUsg040 / BU039 / BBp040 / Bfl562 / transcription antitermination protein
rpoA / 2.7.7.6 / BG10732 / MG177 / b3295 / rpoA / BUsg480 / BU499 / BBp442 / Bfl216 / RNA polymerase, a subunit
rpoB / 2.7.7.6 / BG10728 / MG341 / b3987 / rpoB / BUsg035 / BU034 / BBp035 / Bfl557 / RNA polymerase, b subunit
rpoC / 2.7.7.6 / BG10729 / MG340 / b3988 / rpoC / BUsg034 / BU033 / BBp034 / Bfl556 / RNA polymerase, b' subunit
rpoD / BG10314 / MG249 / b3067 / rpoD / BUsg052 / BU055 / BBp052 / Bfl056 / RNA polymerase major  factor
1.2.2. Translation (96 genes)
1.2.2.1. Aminoacyl-tRNA synthesis (21 genes)
alaS / 6.1.1.7 / BG12563 / MG292 / b2697 / alaS / BUsg390 / BU403 / BBp364 / Bfl168 / alanyl-tRNA synthase
argS / 6.1.1.19 / BG11341 / MG378 / b1876 / argS / BUsg237 / BU242 / BBp224 / Bfl453 / arginyl-tRNA synthase
asnS / 6.1.1.22 / BG10958 / MG113 / b0930 / asnS / BUsg348 / BU360 / BBp329 / Bfl421 / asparaginyl-tRNA synthase
aspS / 6.1.1.12 / BG12572 / MG036 / b1866 / aspS / BUsg306 / BU316 / BBp293 / Bfl452 / aspartyl-tRNA synthase
cysS / 6.1.1.16 / BG10156 / MG253 / b0526 / cysS / BUsg471 / BU487 / BBp431 / Bfl304 / cysteinyl-tRNA synthase
glnS / 6.1.1.18 / b0680 / glnS / BUsg398 / BU415 / BBp375 / Bfl324 / glutaminyl-tRNA synthase
gltX / 6.1.1.17 / BG10154 / MG462 / b2400 / gltX / BUsg065 / BU070 / BBp065 / Bfl504 / glutamyl-tRNA synthase
glyS / 6.1.1.14 / BG11658 / MG251 / b3559 / glyS / BUsg127 / BU135 / BBp126 / Bfl021 / glycyl-tRNA synthase,  subunit
hisS / 6.1.1.21 / BG12605 / MG035 / b2514 / hisS / BUsg277 / BU288 / BBp267 / Bfl531 / histidyl-tRNA synthase
ileS / 6.1.1.5 / BG12605 / MG345(1) / b0026 / ileS / BUsg142 / BU149 / BBp139 / Bfl118 / isoleucyl-tRNA synthase
leuS / 6.1.1.4 / BG10676 / MG266(1) / b0642 / leuS / BUsg429 / BU444 / BBp395 / Bfl313 / leucyl-tRNA synthase
lysS / 6.1.1.6 / BG10144 / MG136 / b2890 / lysS / BUsg422 / BU437 / BBp389 / Bfl262 / lysyl-tRNA synthase
metS / 6.1.1.10 / BG10101 / MG021 / b2114 / metG / BUsg102 / BU109 / BBp103 / Bfl471 / methionyl-tRNA synthase
pheS / 6.1.1.20 / BG10874 / MG194 / b1714 / pheS / BUsg121 / BU129 / BBp123 / Bfl355 / phenylalanyl-tRNA synthase,  subunit
pheT / 6.1.1.20 / BG10875 / MG195 / b1713 / pheT / BUsg122 / BU130 / BBp124 / Bfl356 / phenylalanyl-tRNA synthase,  subunit
proS / 6.1.1.15 / BG12659 / MG283 / b0194 / proS / BUsg234 / BU239 / BBp221 / Bfl289 / prolyl-tRNA synthase
serS / 6.1.1.11 / BG10077 / MG005 / b0893 / serS / BUsg303 / BU313 / BBp290 / Bfl384 / seryl-tRNA synthase
thrS / 6.1.1.3 / BG10362 / MG375 / b1719 / thrS / BUsg117 / BU125 / BBp119 / Bfl351 / threonyl-tRNA synthase
trpS / 6.1.1.2 / BG10799 / MG126 / b3384 / trpS / BUsg517 / BU536 / BBp478 / Bfl569 / tryptophanyl-tRNA synthase
tyrS / 6.1.1.1 / BG10371 / MG455 / b1637 / tyrS / BUsg113 / BU121 / BBp115 / Bfl371 / tyrosyl-tRNA synthase
valS / 6.1.1.9 / BG10321 / MG334 / b4258 / valS / BUsg354 / BU366 / BBp331 / Bfl033 / valyl-tRNA synthase
1.2.2.2. tRNA maturation and modification (6 genes)
mnmAg / 2.1.1.61 / BG13787 / MG295 / b1133 / ycfB / BUsg252 / BU261 / BBp242 / Bfl392 / tRNA (5-methylaminomethyl-2-thiouridylate) methyltransferase
mnmEg / BG10060 / MG008 / b3706 / thdF / BUsg017 / BU016 / BBp017 / Bfl011 / GTP-binding protein involved in biosynthesis of
5-methilaminomethyl-2-thiouridine
mnmGg / BG10059 / MG379 / b3741 / gidA / BUsg001 / BU001 / BBp001 / Bfl001 / glucose inhibited division protein A, involved in biosynthesis of 5-methilaminomethyl-2-thiouridine
rnpA / 3.1.26.5 / BG10063 / MG465 / b3704 / rnpA / BUsg014 / BU014 / BBp014 / Bfl014 / protein component of ribonuclease P
pth / 3.1.1.29 / BG10116 / MG083 / b1204 / pth / BUsg184 / BU190 / BBp179 / Bfl345 / peptidyl-tRNA hydrolase
iscS / 4.4.1.- / BG13809 / MG336 / b2530 / yfhO / BUsg577 / BU602 / BBp544 / Bfl534 / cysteine desulfurase-NifS homolog
1.2.2.3. Ribosomal proteins (50 genes)
rplA / BG10164 / MG082 / b3984 / rplA / BUsg038 / BU037 / BBp038 / Bfl560 / 50S ribosomal protein L1
rplB / BG11217 / MG154 / b3317 / rplB / BUsg502 / BU521 / BBp464 / Bfl194 / 50S ribosomal protein L2
rplC / BG11218 / MG151 / b3320 / rplC / BUsg505 / BU524 / BBp467 / Bfl191 / 50S ribosomal protein L3
rplD / BG11219 / MG152 / b3319 / rplD / BUsg504 / BU523 / BBp466 / Bfl192 / 50S ribosomal protein L4
rplE / BG10760 / MG163 / b3308 / rplE / BUsg493 / BU512 / BBp455 / Bfl203 / 50S ribosomal protein L5
rplF / BG11408 / MG166 / b3305 / rplF / BUsg490 / BU509 / BBp452 / Bfl206 / 50S ribosomal protein L6
rplI / BG10009 / MG093 / b4203 / rplI / BUsg542 / BU562 / BBp508 / Bfl087 / 50S ribosomal protein L9
rplJ / BG11220 / MG361 / b3985 / rplJ / BUsg037 / BU036 / BBp037 / Bfl559 / 50S ribosomal protein L10
rplK / BG10163 / MG081 / b3983 / rplK / BUsg039 / BU038 / BBp039 / Bfl561 / 50S ribosomal protein L11
rplL / BG10726 / MG362 / b3986 / rplL / BUsg036 / BU035 / BBp036 / Bfl558 / 50S ribosomal protein L12
rplM / BG11970 / MG418 / b3231 / rplM / BUsg378 / BU391 / BBp354 / Bfl049 / 50S ribosomal protein L13
rplN / BG10758 / MG161 / b3310 / rplN / BUsg495 / BU514 / BBp457 / Bfl201 / 50S ribosomal protein L14
rplO / BG10444 / MG169 / b3301 / rplO / BUsg486 / BU505 / BBp448 / Bfl210 / 50S ribosomal protein L15
rplP / BG10755 / MG158 / b3313 / rplP / BUsg498 / BU517 / BBp460 / Bfl198 / 50S ribosomal protein L16
rplQ / BG11041 / MG178 / b3294 / rplQ / BUsg479 / BU498 / BBp441 / Bfl217 / 50S ribosomal protein L17
rplR / BG11409 / MG167 / b3304 / rplR / BUsg489 / BU508 / BBp451 / Bfl207 / 50S ribosomal protein L18
rplS / BG12667 / MG444 / b2606 / rplS / BUsg384 / BU397 / BBp360 / Bfl176 / 50S ribosomal protein L19
rplT / BG11971 / MG198 / b1716 / rplT / BUsg120 / BU128 / BBp122 / Bfl354 / 50S ribosomal protein L20
rplU / BG10333 / MG232 / b3186 / rplU / BUsg374 / BU387 / BBp350 / Bfl093 / 50S ribosomal protein L21
rplV / BG11078 / MG156 / b3315 / rplV / BUsg500 / BU519 / BBp462 / Bfl196 / 50S ribosomal protein L22
rplW / BG11221 / MG153 / b3318 / rplW / BUsg503 / BU522 / BBp465 / Bfl193 / 50S ribosomal protein L23
rplX / BG10759 / MG162 / b3309 / rplX / BUsg494 / BU513 / BBp456 / Bfl202 / 50S ribosomal protein L24
rpmA / BG10335 / MG234 / b3185 / rpmA / BUsg375 / BU388 / BBp351 / Bfl094 / 50S ribosomal protein L27
rpmB / BG10777 / MG426 / b3637 / rpmB / BUsg079 / BU086 / BBp080 / Bfl612 / 50S ribosomal protein L28
rpmC / BG10756 / MG159 / b3312 / rpmC / BUsg497 / BU516 / BBp459 / Bfl199 / 50S ribosomal protein L29
rpmE / BG10417 / MG257 / b3936 / rpmE / BUsg556 / BU577 / BBp522 / Bfl599 / 50S ribosomal protein L31
rpmF / BG12668 / MG363 / b1089 / rpmF / BUsg337 / BU349 / BBp319 / Bfl408 / 50S ribosomal protein L32
rpmG / BG10160 / MG325 / b3636 / rpmG / BUsg078 / BU085 / BBp079 / Bfl611 / 50S ribosomal protein L33
rpmH / BG10064 / MG466 / b3703 / rpmH / BUsg013 / BU013 / BBp013 / Bfl015 / 50S ribosomal protein L34
rpmI / BG11972 / MG197 / b1717 / rpmI / BUsg119 / BU127 / BBp121 / Bfl353 / 50S ribosomal protein L35
rpmJ / BG11042 / MG174 / b3299 / rpmJ / BUsg484 / BU503 / BBp446 / Bfl212 / 50S ribosomal protein L36
rpsB / BG19004 / MG070 / b0169 / rpsB / BUsg225 / BU231 / BBp213 / Bfl271 / 30S ribosomal protein S2
rpsC / BG19005 / MG157 / b3314 / rpsC / BUsg499 / BU518 / BBp461 / Bfl197 / 30S ribosomal protein S3
rpsD / BG10372 / MG311 / b3296 / rpsD / BUsg481 / BU500 / BBp443 / Bfl215 / 30S ribosomal protein S4
rpsE / BG10442 / MG168 / b3303 / rpsE / BUsg488 / BU507 / BBp450 / Bfl208 / 30S ribosomal protein S5
rpsF / BG10049 / MG090 / b4200 / rpsF / BUsg544 / BU564 / BBp510 / Bfl085 / 30S ribosomal protein S6
rpsG / BG19006 / MG088 / b3341 / rpsG / BUsg509 / BU528 / BBp471 / Bfl566 / 30S ribosomal protein S7
rpsH / BG10762 / MG165 / b3306 / rpsH / BUsg491 / BU510 / BBp453 / Bfl205 / 30S ribosomal protein S8
rpsI / BG19007 / MG417 / b3230 / rpsI / BUsg377 / BU390 / BBp353 / Bfl050 / 30S ribosomal protein S9
rpsJ / BG19008 / MG150 / b3321 / rpsJ / BUsg506 / BU525 / BBp468 / Bfl190 / 30S ribosomal protein S10
rpsK / BG10731 / MG176 / b3297 / rpsK / BUsg482 / BU501 / BBp444 / Bfl214 / 30S ribosomal protein S11
rpsL / BG19009 / MG087 / b3342 / rpsL / BUsg510 / BU529 / BBp472 / Bfl567 / 30S ribosomal protein S12
rpsM / BG10730 / MG175 / b3298 / rpsM / BUsg483 / BU502 / BBp445 / Bfl213 / 30S ribosomal protein S13
rpsN / BG10761 / MG164 / b3307 / rpsN / BUsg492 / BU511 / BBp454 / Bfl204 / 30S ribosomal protein S14
rpsO / BG19010 / MG424 / b3165 / rpsO / BUsg362 / BU374 / BBp337 / Bfl107 / 30S ribosomal protein S15
rpsP / BG10831 / MG446 / b2609 / rpsP / BUsg381 / BU394 / BBp357 / Bfl173 / 30S ribosomal protein S16
rpsQ / BG10757 / MG160 / b3311 / rpsQ / BUsg496 / BU515 / BBp458 / Bfl200 / 30S ribosomal protein S17
rpsR / BG10047 / MG092 / b4202 / rpsR / BUsg543 / BU563 / BBp509 / Bfl086 / 30S ribosomal protein S18
rpsS / BG19011 / MG155 / b3316 / rpsS / BUsg501 / BU520 / BBp463 / Bfl195 / 30S ribosomal protein S19
rpsT / BG11643 / MG363.1 / b0023 / rpsT / BUsg144 / BU151 / BBp140 / Bfl116 / 30S ribosomal protein S20
1.2.2.4. Ribosome function, maduration and modification (7 genes)
ksgA / 2.1.1.- / BG10105 / MG463 / b0051 / ksgA / BUsg134 / BU141 / BBp131 / Bfl126 / dimethyladenosine transferase
rbfA / BG10270 / MG143 / b3167 / rbfA / BUsg364 / BU376 / BBp339 / Bfl105 / ribosome-binding factor A
engA / BG11443 / MG329 / b2511 / b2511 / BUsg582 / BU607 / BBp549 / Bfl530 / GTP-binding protein
obg / BG10337 / MG384 / b3183 / yhbZ / BUsg376 / BU389 / BBp352 / Bfl095 / GTP-binding protein
ychF / BG10050 / MG024 / b1203 / ychF / BUsg185 / BU191 / BBp180 / Bfl344 / GTP-binding protein
era / BG11188 / MG387 / b2566 / BUsg248 / BU257 / BBp238 / GTP-binding protein
cspRh / 2.1.1.- / BG11802 / MG346 / b3606 / ftsJ / BUsg370 / BU383 / BBp346 / Bfl097 / ribosomal methytransferase
1.2.2.5. Translation factors (12 genes)
efp / BG11460 / MG026 / b4147 / efp / BUsg021 / BU020 / BBp023 / Bfl072 / elongation factor P
fusA / 3.6.1.48 / BG11939 / MG089 / b3340 / fusA / BUsg508 / BU527 / BBp470 / Bfl565 / elongation factor G
frr / BG12587 / MG435 / b0172 / frr / BUsg228 / BU234 / BBp216 / Bfl274 / ribosome recycling factor
hemK / 2.1.1.- / BG11316 / MG259 / b1212 / hemK / BUsg166 / BU172 / BBp162 / Bfl349 / N5-glutamine methyltransferase, modulation of release factors activity
infA / BG11043 / MG173 / b0884 / infA / BUsg305 / BU315 / BBp292 / Bfl388 / initiation factor IF-1
infB / BG10268 / MG142 / b3168 / infB / BUsg365 / BU377 / BBp340 / Bfl104 / initiation factor IF-2
infC / BG11944 / MG196 / b1718 / infC / BUsg118 / BU126 / BBp120 / Bfl352 / initiation factor IF-3
lepA / BG10441 / MG138 / b2569 / lepA / BUsg251 / BU260 / BBp241 / Bfl542 / GTP-binding elongation factor
prfA / BG11244 / MG258 / b1211 / prfA / BUsg165 / BU171 / BBp161 / Bfl348 / peptide chain release factor 1 (RF1)
smpB / BG14064 / MG059 / b2620 / smpB / BUsg245 / BU254 / BBp235 / Bfl548 / tmRNA-binding protein
tsf / BG19025 / MG433 / b0170 / tsf / BUsg226 / BU232 / BBp214 / Bfl272 / elongation factor Ts
tufA / 3.6.5.3 / BG11056 / MG451 / b3980 / tufA / BUsg507 / BU526 / BBp469 / Bfl564 / elongation factor Tu
1.2.3. RNA degradation (2 genes)
pnp / 2.7.7.8 / BG11491 / b3164 / pnp / BUsg361 / BU373 / BBp336 / Bfl108 / polyribonucleotide nucleotidyltransferase
rnc / 3.1.26.3 / BG11537 / MG367 / b2567 / rnc / BUsg249 / BU258 / BBp239 / Bfl540 / ribonuclease III
2. PROTEIN PROCESSING, FOLDING, AND SECRETION (15 genes)
2.1. Protein post-translational modification (2 genes)
map / 3.4.11.18 / BG10447 / MG172 / b0168 / map / BUsg224 / BU230 / BBp212 / Bfl270 / methionine aminopeptidase
pepA / 3.4.11.1 / BG13970 / MG391 / b4260 / pepA / BUsg355 / BU367 / BBp332 / Bfl035 / aminopeptidase A/I
2.2. Protein folding (5 genes)
dnaJ / BG10665 / MG019 / b0015 / dnaJ / BUsg145 / BU152 / BBp141 / Bfl115 / Hsp70 co-chaperone
dnaK / BG10664 / MG305 / b0014 / dnaK / BUsg146 / BU153 / BBp142 / Bfl114 / chaperone Hsp70
groEL / BG10423 / MG392 / b4143 / mopA / BUsg019 / BU019 / BBp021 / Bfl071 / class I heat-shock protein
groES / BG10422 / MG393 / b4142 / mopB / BUsg018 / BU018 / BBp020 / Bfl070 / class I heat-shock protein
grpE / BG10663 / MG201 / b2614 / grpE / BUsg178 / BU184 / BBp173 / Bfl544 / Hsp70 co-chaperone
2.3. Protein translocation and secretion (5 genes)
ffh / BG10830 / MG048 / b2610 / ffh / BUsg380 / BU393 / BBp356 / Bfl172 / protein component of signal recognition particle (SRP)
ftsY / BG11539 / MG297 / b3464 / ftsY / BUsg025 / BU024 / BBp026 / Bfl627 / signal recognition particle receptor
secA / BG10741 / MG072 / b0098 / secA / BUsg195 / BU201 / BBp187 / Bfl148 / preprotein translocase subunit (ATPase)
secE / BG10161 / MG055 / b3981 / secE / BUsg041 / BU040 / BBp041 / Bfl563 / membrane-embebed preprotein translocase subunit
secY / BG10445 / MG170 / b3300 / prlA / BUsg485 / BU504 / BBp447 / Bfl211 / membrane-embebed preprotein translocase subunit
2.4. Protein turnover (3 genes)
gcp / 3.4.24.57 / BG12202 / MG046 / b3064 / ygjD / BUsg055 / BU058 / BBp055 / Bfl059 / probable O-sialoglycoprotein endopeptidase
hflB / 3.4.24.- / BG10132 / MG457 / b3178 / hflB / BUsg369 / BU382 / BBp345 / Bfl098 / ATP-dependent protease
lon / 3.4.21.53 / BG10338 / MG231 / b0439 / lon / BUsg461 / BU477 / BBp421 / Bfl299 / ATP-dependent protease La
3. CELLULAR PROCESSES (5 genes)
3.1. Cell division (1 gene)
ftsZ / BG10232 / MG224 / b0095 / ftsZ / BUsg206 / BU212 / BBp194 / Bfl146 / cytoskeletal cell division protein
3.2. Transport (4 genes)
pitA / BG11253 / b3493 / pitA / BUsg566 / BU587 / BBp531 / Bfl024 / low-affinity inorganic phosphate transporter
ptsG / 2.7.1.69 / BG10198 / MG069 / b1101 / BUsg344 / BU356 / BBp326 / phosphotransferase system (PTS) glucose-specific enzyme II
ptsH / BG10200 / MG041 / b2415 / BUsg062 / BU065 / BBp061 / Bfl509 / histidine-containing phosphocarrier protein of the
phosphotransferase system (PTS)
ptsI / 2.7.3.9 / BG10201 / MG429 / b2416 / BUsg061 / BU064 / BBp060 / Bfl510 / phosphotransferase system (PTS) enzyme I
4. ENERGETIC AND INTERMEDIARY METABOLISM (56 genes)
4.1. Glycolysis (10 genes)
eno / 4.2.1.11 / BG10899 / MG407 / b2779 / eno / BUsg400 / BU417 / BBp377 / Bfl157 / enolase
fbaA / 4.1.2.13 / BG10412 / MG023 / b2925 / fba / BUsg436 / BU451 / BBp401 / Bfl255 / fructose-1,6-bisphosphate aldolase
gapA / 1.2.1.12 / BG10827 / MG301 / b1779 / gapA / BUsg287 / BU298 / BBp276 / Bfl437 / glyceraldehyde 3-phosphate dehydrogenase
gpmAi / 5.4.2.1 / BG10898 / MG430 / b0755 / gpmA / BUsg294 / BU304 / BBp283 / Bfl342 / phosphoglycerate mutase
ldh / 1.1.1.27 / BG19003 / MG460 / b1380 / L-lactate dehydrogenase
pfkA / 2.7.1.11 / BG12644 / MG215 / b3916 / BUsg295 / BU305 / BBp284 / Bfl602 / 6-phosphofructokinase
pgi / 5.3.1.9 / BG12366 / MG111 / b4025 / pgi / BUsg553 / BU573 / BBp518 / Bfl629 / glucose-6-phosphate isomerase
pgk / 2.7.2.3 / BG11062 / MG300 / b2926 / pgk / BUsg435 / BU450 / BBp400 / Bfl254 / phosphoglycerate kinase
pykA / 2.7.1.40 / BG12661 / MG216 / b1854 / pykA / BUsg311 / BU319 / BBp297 / Bfl450 / pyruvate kinase
tpiA / 5.3.1.1 / BG10897 / MG431 / b3919 / tpiA / BUsg297 / BU307 / BBp285 / Bfl601 / triose phosphate isomerase
4.2. Proton-motive force generation (9 genes)
atpA / 3.6.3.14 / BG10819 / MG401 / b3734 / atpA / BUsg006 / BU006 / BBp006 / Bfl006 / ATP synthase  chain
atpB / 3.6.3.15 / BG10815 / MG405 / b3738 / atpB / BUsg002 / BU002 / BBp002 / Bfl002 / ATP synthase A chain
atpC / 3.6.3.16 / BG10822 / MG398 / b3731 / atpC / BUsg009 / BU009 / BBp009 / Bfl009 / ATP synthase  chain
atpD / 3.6.3.17 / BG10821 / MG399 / b3732 / atpD / BUsg008 / BU008 / BBp008 / Bfl008 / ATP synthase  chain
atpE / 3.6.3.18 / BG10816 / MG404 / b3737 / atpE / BUsg003 / BU003 / BBp003 / Bfl003 / ATP synthase C chain
atpF / 3.6.3.19 / BG10817 / MG403 / b3736 / atpF / BUsg004 / BU004 / BBp004 / Bfl004 / ATP synthase B chain
atpG / 3.6.3.20 / BG10820 / MG400 / b3733 / atpG / BUsg007 / BU007 / BBp007 / Bfl007 / ATP synthase  chain
atpH / 3.6.3.21 / BG10818 / MG402 / b3735 / atpH / BUsg005 / BU005 / BBp005 / Bfl005 / ATP synthase  chain
yidC / BG10062 / MG464 / b3705 / yidC / BUsg016 / BU015 / BBp016 / Bfl012 / essential for proper integration of ATPase into the membrane
4.3. Penthose phosphate pathway (3 genes)
rpe / 5.1.3.1 / BG13393 / MG112 / b3386 / rpe / BUsg518 / BU537 / BBp479 / Bfl570 / ribulose-phosphate 3-epimerase
rpiAj / 5.3.1.6 / BG10942 / MG396 / b2914 / rpiA / BUsg397 / BU411 / BBp371 / Bfl256 / ribose 5-phosphate isomerase
tkt / 2.2.1.1 / BG11247 / MG066 / b2465 / tktB / BUsg086 / BU094 / BBp088 / Bfl516 / transketolase
4.5. Lipid metabolism (7 genes)
cdsA / 2.7.7.41 / BG12575 / MG437 / b0175 / cdsA / Bfl277 / phosphatidate cytidylyltransferase
fadD / 6.2.1.3 / BG11946 / b1805 / acyl-CoA synthase
gpsA / 1.1.1.94 / BG11366 / b3608 / gpsA / Bfl604 / sn-glycerol-3-phosphate dehydrogenase
plsB / 2.3.1.15 / b4041 / plsB / sn-glycerol-3-phosphate acyltransferase
plsC / 2.3.1.51 / BG13021 / MG212 / b3018 / plsC / Bfl066 / 1-acyl-sn-glycerol-3-phosphate acyltransferase
psd / 4.1.1.65 / BG11013 / b4160 / psd / Bfl074 / phosphatidylserine decarboxylase
pssA / 2.7.8.8 / BG11012 / b2585 / pssA / Bfl551 / phosphatidylserine synthase
4.4. Biosynthesis of nucleotides (15 genes)
adk / 2.7.4.3 / BG10446 / MG171 / b0474 / adk / BUsg469 / BU484 / BBp428 / Bfl302 / adenylate kinase
dcd / 3.5.4.13 / b2065 / dcd / BUsg101 / BU108 / BBp102 / dCTP deaminase
gmk / 2.7.4.8 / BG13386 / MG107 / b3648 / gmk / BUsg419 / BU434 / BBp385 / Bfl616 / guanylate kinase
hpt / 2.4.2.8 / BG10131 / MG458 / b0125 / BUsg189 / BU195 / hypoxanthine phosphoribosyltransferase
ndk / 2.7.4.6 / BG10282 / b2518 / ndk / Bfl533 / nucleoside diphosphate kinase
nrdE / 1.17.4.1 / BG11404 / MG231 / b2675 / nrdE / BUsg173 / BU179 / BBp168 / Bfl478 / ribonucleoside-diphosphate reductase (major subunit)
nrdF / 1.17.4.1 / BG11405 / MG229 / b2676 / nrdF / BUsg172 / BU178 / BBp167 / Bfl479 / ribonucleoside-diphosphate reductase (minor subunit)
ppa / 3.6.1.1 / BG10014 / MG351 / b4226 / ppa / BUsg081 / BU088 / BBp082 / Bfl091 / inorganic pyrophosphatase
prsA / 2.7.6.1 / BG10114 / MG058 / b1207 / prsA / BUsg163 / BU169 / BBp159 / Bfl346 / phosphoribosylpyrophosphate synthase
pyrG / 6.3.4.2 / BG10410 / b2780 / pyrG / BUsg399 / BU416 / BBp376 / Bfl156 / CTP synthase
thyA / 2.1.1.45 / BG11190 / MG227 / b2827 / thyA / BUsg425 / BU440 / BBp386 / thymidylate synthase
tmk / 2.7.4.9 / BG10092 / MG006 / b1098 / tmk / BUsg341 / BU353 / BBp323 / Bfl401 / thymidylate kinase
trxA / BG10348 / MG124 / b3781 / trxA / BUsg573 / BU597 / BBp539 / Bfl587 / thioredoxin
trxB / 1.8.1.9 / BG12398 / MG102 / b0888 / trxB / BUsg304 / BU314 / BBp291 / Bfl387 / thioredoxin reductase
upp / 2.4.2.9 / BG10945 / MG030 / b2498 / Bfl520 / uracil phosphoribosyltransferase
4.5. Biosynthesis of cofactors (12 genes)
coaA / 2.7.1.33 / BG11748 / b3974 / coaA / pantothenate kinase
coaD / 2.7.7.3 / BG13361 / b3634 / kdtB / BUsg562 / BU583 / 4'-phospho-pantetheine adenylyltransferase
coaE / 2.7.1.24 / BG13824 / MG264 / b0103 / yacE / BUsg197 / BU203 / dephosphocoenzyme A kinase
dfp / 6.3.2.5 4.1.1.36 / BG13388 / b3639 / dfp / phosphopantothenate cysteine ligase,
4'-phospho-pantothenyl-L-cysteine decarboxylase
folA / 1.5.1.3 / BG10795 / MG228 / b0048 / folA / BUsg136 / BU143 / BBp133 / Bfl124 / dihydrofolate reductase
glyA / 2.1.2.1 / BG10944 / MG394 / b2551 / glyA / BUsg278 / BU289 / BBp268 / Bfl536 / glycine hydroxymethyltransferase
metK / 2.5.1.6 / BG11840 / MG047 / b2942 / metK / BUsg393 / BU408 / Bfl252 / methionine adenosyltransferase
nadR / 2.7.7.1 / b4390 / adenilyl transferase
nadV / 2.4.2.12 / nicotinamide phosphoribosyltransferase
pdxY / 2.7.1.35 / b1636 / pyridoxal kinase
ribF / 2.7.1.262.7.1.26 2.7.7.2 / BG11495 / MG145 / b0025 / ribF / BUsg143 / BU150 / Bfl117 / riboflavin kinase, FMN adenylyltransferase
yloS / 2.7.6.2 / BG13394 / thiamine pyrophosphokinase
5. POORLY CHARACTERIZED (8 genes)
mesJ / BG10130 / MG084 / b0188 / mesJ / BUsg103 / BU110 / BBp104 / Bfl288 / conserved hypothetical protein
mraW / 2.1.1.- / BG10219 / MG222 / b0082 / yabC / BUsg218 / BU224 / BBp206 / Bfl134 / methyltrasferase
ybeY / BG11656 / MG388 / b0659 / ybeY / BUsg427 / BU442 / BBp393 / Bfl316 / conserved hypothetical protein
ycfF / BG12607 / MG132 / b1103 / ycfF / BUsg345 / BU357 / BBp327 / Bfl398 / HIT family
ycfH / 3.1.21.- / BG10102 / MG009 / b1100 / ycfH / BUsg343 / BU355 / BBp325 / Bfl399 / putative deoxyribonuclease, tatD family
yoaE / BG12371 / MG146 / b1816 / yoaE / BUsg314 / BU323 / BBp300 / Bfl444 / conserved hypothetical protein
yqgF / BG13792 / MG291.1 / b2949 / yqgF / BUsg530 / BU548 / BBp492 / Bfl250 / conserved hypothetical protein
yraL / BG10099 / MG056 / b3146 / yraL / BUsg083 / BU091 / BBp085 / Bfl052 / conserved hypothetical protein

a Proteins encoded by BG10263 and MG031 contain an  subunit as an internal domain.

b Similarity between B. subtilis, M. genitalium and -Proteobacterial proteins is extremely low.

c Bfl280 (hplA) encodes the only DNA-binding protein present in Bl. floridanus, but it has no homology with hupA

d MG235 (nfo) encodes a different but functionally equivalent exonuclease (EC 3.1.21.2)

e The 5’-3’ exonuclease activity is a domain of the PolA protein in B. subtilis, E. coli and B. floridanus. In the rest of species the gene does not encode more domains.

f BUsg177 (ung) contains an authentic frameshift in the 5’ end of the gene. However, an ORF of 170 amino acids versus the 220 amino acids of the original gene can be detected. Further analysis will be required to confirm functionality of this truncated protein.

gold genes names trmU (mnmA), thdF (mnmE) and gidA (mnmG).

h ftsJ is the only ribosomal methylase present in all five endosymbionts. The gene, which is also present in E. coli, is not orthologous to cspR, although it might be functionally equivalent in these endosymbionts.

i Enzyme activity EC. 5.4.2.1 is encoded by non homologous genes in gram-positive and gram-negative species.

j Enzyme activity EC. 5.3.1.6 is encoded by the non-homologous genes rpiA (b2914, BUsg397, BU411, BBp371, Bfl256 and W. glossinidia) and rpiB (BG10942and MG396). E. coli also contains the rpiB gene (b4090).