Table 1: Primers Used for the Detection of Antimicrobial Resistance Genes in E
Table 1: Primers used for the detection of antimicrobial resistance genes in E. coli isolates.
Gene Name / Antimicrobialresistance / Primer name / Sequence (5’ 3’) / Amplicon size(bp) / Reference
tetA
tetB
tetC
SHV
OXA
TEM
aadA
sull
dhfrV
dhfrI / Tetracycline
Tetracycline
Tetracycline
Beta-lactam
Beta-lactam
Beta-lactam
Aminoglycoside
Sulfonamide
Trimethoprim
Trimethoprim / tetA(F)
tetA(R)
tetB(F)
tetB(R)
tetC(F)
tetC(R)
blaSHV-F
blaSHV-R
blaOXA-F
blaOXA-R
blaTEM-F
blaTEM-R
aadA-F
aadA-R
sull-F
sull-R
dhfrV-F
dhfrV-R
dhfrI-F
dhfrI-R / GTGAAACCCAACATACCCC
GAAGGCAAGCAGGATGTAG
CCTTATCATGCCAGTCTTGC
ACTGCCGTTTTTTCGCC
ACTTGGAGCCACTATCGAC
CTACAATCCATGCCAACCC
TCGCCTGTGTATTATCTCCC
CGCAGATAAATCACCACAATG
GCAGCGCCAGTGCATCAAC
CCGCATCAAATGCCATAAGTG
GAGTATTCAACATTTTCGT
ACCAATGCTTAATCAGTGA
TGATTTGCTGGTTACGGTGAC
CGCTATGTTCTCTTGCTTTTG
TTCGGCATTCTGAATCTCAC
ATGATCTAACCCTCGGTCTC
CTGCAAAAGCGAAAAACGG
AGCAATAGTTAATGTTTGAGCTAAAG
AAGAATGGAGTTATCGGGAATG
GGGTAAAAACTGGCCTAAAATTG / 887
773
880
768
198
857
284
822
432
391 / Van et al. 2008
Van et al. 2008
Van et al. 2008
Van et al. 2008
Van et al. 2008
Van et al. 2008
Van et al. 2008
Van et al. 2008
Van et al. 2008
Van et al. 2008
Table 2: Phenotypes of resistance and genes of resistance detected among the 102 antibiotic resistant isolates of E.coli recovered from pigs
Phenotype of resistance / Number of isolates / Genes detected by PCRGene / Number of isolates (%)
Tetracycline
Ampicillin
Streptomycin
SXT1 / 102
3
31
6 / tet(A)
tet(B)
tet(A)+ tet(B)
none
aadA
sul1
dhfrV / 71(69.6)
9(8.8)
1(0.9)
14(45.1)
2(33.3)
1(16.6)
1 SXT: sulfamethoxazole-trimethoprim
Table 3. Phenotypes of resistance patterns and phylogenetic groups detected among the 102 antibiotic resistant isolates of E.coli recovered from pigs
Phenotype ofresistance pattern / Number of isolates / Phylogenetic group
A / B1 / B2 / D
TE
TE+S
TE+AM
TE+SXT
TE+AM+S
TE+SXT+S / 65
28
1
5
2
1 / 41
15
-
2
2
1 / 11
5
-
1
-
- / 1
3
-
1
-
- / 12
5
-
1
-
-
Total
resistant isolates / 102 / 61 / 18 / 5 / 18
TE: tetracycline; S: streptomycin; AM: ampicillin; SXT: sulfamethoxazole-trimethoprim