SUPPLEMENTARYMATERIAL (online only)
SUPPLEMENTARY TABLES
TableS1 Summary of differentially expressed genes associated with prenylated PTP activity during cancer progression in the BC transcriptome (GSE32894)
Probe / Comparing T2-4 to Ta-1 / Gene Symbol / Biological Process / Molecular FunctionLog ratio / P value
ILMN_1662427 / 0.1478 / 0.0019 / PTP4A3 / protein amino acid dephosphorylation / hydrolase activity, identical protein binding, prenylated protein tyrosine phosphatase activity
ILMN_1769779 / 0.2034 / 0.0002 / PTP4A3 / protein amino acid dephosphorylation / hydrolase activity, identical protein binding, prenylated protein tyrosine phosphatase activity
ILMN_2359710 / 0.1826 / 0.0005 / PTP4A3 / protein amino acid dephosphorylation / hydrolase activity, identical protein binding, prenylated protein tyrosine phosphatase activity
ILMN_2338997 / -0.2915 / 0.0001 / PTP4A2 / protein amino acid dephosphorylation / hydrolase activity, prenylated protein tyrosine phosphatase activity, protein binding
TableS2Associationbetween PTP4A3 expression and clinicopathological factors ofMIBC
Parameter / Category / Case No. / PTP4A3 Expression / PvalueLow / High
Gender, no. (%) / Male / 91 (74) / 29 (32) / 62 (68) / 0.795
Female / 32 (26) / 11 (34) / 21 (66)
Age (years), no. (%) / < 65 / 51 (41) / 13 (25) / 38 (75) / 0.161
≥ 65 / 72 (59) / 27 (37) / 93 (63)
Primary tumor (T), no. (%) / T2 / 53 (43) / 26 (49) / 27 (51) / 0.003*
T3 / 49 (40) / 10 (20) / 39 (80)
T4 / 21 (17) / 4 (19) / 17 (81)
Nodal metastasis, no. (%) / Negative (N0) / 94 (76) / 35 (37) / 59 (63) / 0.045*
Positive (N1-2) / 29 (24) / 5 (17) / 24 (83)
Histological grade, no. (%) / Low grade / 2 (2) / 1 (50) / 1 (50) / 0.546
High grade / 121 (98) / 39 (32) / 82 (68)
Vascular invasion, no. (%) / Absent / 75 (61) / 27 (36) / 48 (64) / 0.303
Present / 48 (39) / 13 (27) / 35 (73)
Perineural invasion, no. (%) / Absent / 106 (86) / 36 (34) / 70 (66) / 0.394
Present / 17 (14) / 5 (24) / 13 (76)
Mitotic rate (per 10 high power fields), no. (%) / < 10 / 45 (37) / 16 (36) / 29 (64) / 0.585
≥ 10 / 78 (63) / 24 (31) / 54 (69)
* Statistically significant
TableS3Associationbetween PTP4A3 expression and clinicopathological factors ofNMIBC
Parameter / Category / Case No. / PTP4A3 Expression / PvalueLow / High
Gender, no. (%) / Male / 125 (73) / 76 (61) / 49 (39) / 0.534
Female / 47 (27) / 31 (66) / 16 (34)
Age (years), no. (%) / < 65 / 70 (41) / 53 (76) / 17 (24) / 0.002*
≥ 65 / 102 (59) / 54 (53) / 48 (47)
Primary tumor (T), no. (%) / Ta / 84 (49) / 44 (52) / 40 (48) / 0.009*
T1 / 88 (51) / 63 (72) / 25 (28)
Nodal metastasis, no. (%) / Negative (N0) / 172 (100) / 107 (62) / 65 (38) / -
Positive (N1-2) / 0 (0) / 0 (0) / 0 (0)
Histological grade, no. (%) / Low grade / 54 (31) / 33 (61) / 21 (39) / 0.841
High grade / 1118 (69) / 74 (63) / 44 (37)
Vascular invasion, no. (%) / Absent / 171 (99) / 107 (63) / 64 (37) / 0.378
Present / 1 (1) / 0 (0) / 1 (100)
Perineural invasion, no. (%) / Absent / 169 (98) / 106 (63) / 63 (37) / 0.558
Present / 3 (2) / 1 (33) / 2 (67)
Mitotic rate (per 10 high power fields), no. (%) / < 10 / 94 (55) / 52 (55) / 42 (45) / 0.041*
≥ 10 / 78 (45) / 55 (71) / 23 (29)
* Statistically significant
TableS4Univariate and multivariate analyses predicting CSS and MFS in patients with MIBC
Parameter / Category / Case No. / CSS / MFSUnivariate analysis / Multivariate analysis / Univariate analysis / Multivariate analysis
No. of event / Pvalue / H.R. / 95% C.I. / Pvalue / No. of event / Pvalue / H.R. / 95% C.I. / Pvalue
Gender / Male / 91 / 35 / 0.2626 / - / - / - / 41 / 0.1618 / - / - / -
Female / 32 / 7 / - / - / - / 8 / - / - / -
Age (years) / < 65 / 51 / 14 / 0.2755 / - / - / - / 19 / 0.6554 / - / - / -
≥ 65 / 72 / 28 / - / - / - / 30 / - / - / -
Primary tumor (T) / T2 / 53 / 17 / 0.4798 / - / - / - / 16 / 0.0625 / - / - / -
T3-4 / 70 / 25 / - / - / - / 33 / - / - / -
Nodal metastasis / Negative (N0) / 94 / 31 / 0.4131 / - / - / - / 34 / 0.0775 / - / - / -
Positive (N1-2) / 29 / 11 / - / - / - / 15 / - / - / -
Histological grade / Low grade / 2 / 0 / 0.4294 / - / - / - / 0 / 0.4361 / - / - / -
High grade / 121 / 42 / - / - / - / 49 / - / - / -
Vascular invasion / Absent / 75 / 27 / 0.7836 / - / - / - / 27 / 0.3012 / - / - / -
Present / 48 / 15 / - / - / - / 22 / - / - / -
Perineural invasion / Absent / 106 / 34 / 0.0385* / 1 / 0.019* / 39 / 0.0421* / 1 / 0.033*
Present / 17 / 8 / 2.602 / 1.167-5.801 / 10 / 2.162 / 1.065-4.391
Mitotic rate (per 10 high power fields) / < 10 / 45 / 5 / 0.0008* / 1 / 0.001* / 11 / 0.0085* / 1 / 0.013*
≥ 10 / 78 / 37 / 4.626 / 1.814-11.798 / 38 / 2.354 / 1.200-4.616
PTP4A3 expression / Low / 40 / 8 / 0.0077* / 1 / 0.005* / 10 / 0.0137* / 1 / 0.013*
High / 83 / 34 / 3.178 / 1.414-7.141 / 39 / 2.463 / 1.214-4.998
* Statistically significant
TableS5Univariate and multivariate analyses predicting CSS, MFS, and RFS in patients with NMIBC
Parameter / Category / Case No. / CSS / MFS / RFSUnivariate analysis / Multivariate analysis / Univariate analysis / Multivariate analysis / Univariate analysis / Multivariate analysis
Event / Pvalue / H.R. / 95% C.I. / Pvalue / Event / Pvalue / H.R. / 95% C.I. / Pvalue / Event / Pvalue / H.R. / 95% C.I. / Pvalue
Gender / Male / 125 / 6 / 0.3126 / - / - / - / 19 / 0.7298 / - / - / - / 46 / 0.3389 / - / - / -
Female / 47 / 4 / - / - / - / 8 / - / - / - / 19 / - / - / -
Age (years) / < 65 / 70 / 3 / 0.3463 / - / - / - / 12 / 0.9451 / - / - / - / 30 / 0.3871 / - / - / -
≥ 65 / 102 / 7 / - / - / - / 15 / - / - / - / 35 / - / - / -
Primary tumor (T) / Ta / 84 / 1 / 0.0286* / 1 / 0.021* / 4 / 0.0003* / 1 / <0.001* / 27 / 0.0212* / 1 / 0.350
T1 / 88 / 9 / 11.445 / 1.444-90.681 / 23 / 7.000 / 2.418-20.265 / 38 / 1.331 / 0.730-2.427
Nodal metastasis / Negative / 172 / 10 / - / - / - / - / 27 / - / - / - / - / 65 / - / - / - / -
Positive / 0 / 0 / - / - / - / 0 / - / - / - / 0 / - / - / -
Histological grade / Low grade / 54 / 2 / 0.3453 / - / - / - / 5 / 0.0699 / - / - / - / 15 / 0.0121* / 1 / 0.109
High grade / 118 / 8 / - / - / - / 22 / - / - / - / 50 / 1.789 / 0.878-3.646
Vascular invasion / Absent / 171 / 10 / 0.9401 / - / - / - / 27 / 0.9165 / - / - / - / 65 / 0.7719 / - / - / -
Present / 1 / 0 / - / - / - / 0 / - / - / - / 0 / - / - / -
Perineural invasion / Absent / 169 / 10 / 0.7685 / - / - / - / 27 / 0.6130 / - / - / - / 64 / 0.4824 / - / - / -
Present / 3 / 0 / - / - / - / 0 / - / - / - / 1 / - / - / -
Mitotic rate (per 10 high power fields) / < 10 / 94 / 7 / 0.5562 / - / - / - / 12 / 0.0587 / - / - / - / 35 / 0.1877 / - / - / -
≥ 10 / 78 / 3 / - / - / - / 15 / - / - / - / 30 / - / - / -
PTP4A3 expression / Low / 107 / 2 / 0.0384* / 1 / 0.036* / 14 / 0.9303 / - / - / - / 40 / 0.3258 / - / - / -
High / 65 / 8 / 5.348 / 1.114-25.689 / 13 / - / - / - / 25 / - / - / -
* Statistically significant
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