Supplementaryfig. 4 PCR Quantification Report

(a)

Transgenic / Control (wild type)
Primer
landing
region / Concentration
of DNA (ng) / Ct / Primer
landing
region / Concentration
of DNA (ng) / Ct
NtWBC / 100 / 22.79 / NtWBC / 100 / 22.33
NtWBC / 100 / 22.99 / NtWBC / 100 / 22.56
NtWBC / 50 / 23.64 / NtWBC / 50 / 23.12
NtWBC / 50 / 23.63 / NtWBC / 50 / 22.99
NtWBC / 25 / 24.37 / NtWBC / 25 / 24.1
NtWBC / 25 / 24.47 / NtWBC / 25 / 23.9
HSigi / 100 / 22.08 / HSigi / 100 / 29.26
HSigi / 100 / 22.1 / HSigi / 100 / 27.9
HSigi / 50 / 23.57 / HSigi / 50 / 29.7
HSigi / 50 / 23.11 / HSigi / 50 / 29.4
HSigi / 25 / 24.02 / HSigi / 25 / 30.78
HSigi / 25 / 23.92 / HSigi / 25 / 30.98
Sig32 / 100 / 22.82 / Sig32 / 100 / 33.17
Sig32 / 100 / 22.85 / Sig32 / 100 / 31.59
Sig32 / 50 / 23.71 / Sig32 / 50 / 32.93
Sig32 / 50 / 24.03 / Sig32 / 50 / 32.17
Sig32 / 25 / 24.72 / Sig32 / 25 / 34.63
Sig32 / 25 / 24.92 / Sig32 / 25 / 33.62
SigA / 100 / 19.98 / SigA / 100 / 19.8
SigA / 100 / 19.83 / SigA / 100 / 20.11
SigA / 50 / 20.57 / SigA / 50 / 20.4
SigA / 50 / 20.89 / SigA / 50 / 20.68
SigA / 25 / 21.69 / SigA / 25 / 21.61
SigA / 25 / 21.79 / SigA / 25 / 21.42

(b)

SupplementaryFig. 4 PCR quantification report.

(a) PCR quantification spreadsheet data for SYBR-490; (b) PCR Amp/Cycle graph for SYBR-490 (NTC: nontemplate control)