Supplementary Table 1A; EST Derived SSR Primers
Supplementary Table 1a; EST derived SSR primers
Chromosome / Marker / Repeat / Forward Primer5’ to 3’ / Reverse Primer
5’ to 3’ / Primer reference
Nuclear (4a) / DpW004 / AC / GGTCTGGTCGGAGAAGAAGC / TGGGAGCGTACGTTGTATCC / Eujaylet al., 2001
Nuclear (4b) / DpW023 / GCT / TTGCTCCGATGTAATAAGCG / GGCTAAGAACAGACTCATTCAACTG / Eujaylet al., 2001
Nuclear (1a) / DpW038 / GCG / ATTAGACACGACCAAACGGG / TCAAACAAACAACAGCCAGC / P. Wolters, pers. comm.
Nuclear (4a) / DpW108 / AC:ATC / TGAAGAGTGCGATGTGAAGG / TGTGACAGAAACTACTAACATTGCG / Eujaylet al., 2001
Nuclear (5b) / DpW115 / ACG / TGTTTCTTCCTCGCGTAACC / CCTCGAATCTCCCAGTTATCG / Eujaylet al.., 2001
Nuclear (5b) / DpW205 / AAG / ATCCAGATCACACCAAACGG / CTTCCGCTTCATCTTCTTGC / Eujaylet al., 2001
Nuclear(7a) / DpW226 / AAG / GGAGTAGGAATCAGGGCTGC / ACATGAGGCGAACCTTGC / P. Wolters, pers. comm.
Nuclear (7a) / DpW254 / AC / TTAACCATGCAGCAACTTCG / GTGTGTACTAACGGCTACGGC / Eujaylet al., 2001
Nuclear (7b) / DpW276 / ACAT / AGAGAGAGACAGACGGACGG / CGTTCTCTTCATTATTTACCGGC / P. Wolters, pers. comm.
Nuclear (6a) / DpW309 / ACG / CATTCCCAGGTTGGATGC / CACCTTCACCTTGAGCAGC / P. Wolters, pers. comm.
Nuclear (1a) / DpW330 / ACT / TTCATCTCCTACACGCACCC / CAGTTCAATCCAGACCCTGC / P. Wolters, pers. comm.
Nuclear (6b) / DpW378 / AG / TTCATCAGACAAGGTGGTGC / GCAAGAATTGCTGTTGCTACC / P. Wolters, pers. comm.
Supplementary Table 1b; Genomic SSR Primers
Chromosome / Marker / Repeat / Forward Primer5’ to 3’ / Reverse Primer
5’ to 3’ / Primer reference
(1b) / Xgwm18 / (CA)GA(TA) / TGG CGC CAT GAT TGC ATT ATC TTC / GGT TGC TGA AGA ACC TTA TTT AGG / Roder et al. 1998
(7b) / Xgwm46 / (GA)GC(GA) / GCA CGT GAA TGG ATT GGA C / TGA CCC AAT AGT GGT GGT CA / Roder et al. 1998
(7a) / Xgwm60 / CA / TGT CCT ACA CGG ACC ACG T / GCA TTG ACA GAT GCA CAC G / Roder et al. 1998
(2a) / Xgwm95 / AC / GAT CAA ACA CAC ACC CCT CC / AAT GCA AAG TGA AAA ACC CG / Roder et al. 1998
(2b) / Xgwm120 / (CT)(CA) / GAT CCA CCT TCC TCT CTC TC / GAT TAT ACT GGT GCC GAA AC / Roder et al. 1998
(1b) / Xgwm124 / (CT)(GT)imp / GCC ATG GCT ATC ACC CAG / ACT GTT CGG TGC AAT TTG AG / Roder et al. 1998
(1a) / Xgwm135 / GA / TGT CAA CAT CGT TTT GAA AAG G / ACA CTG TCA ACC TGG CAA TG / Roder et al. 1998
(5a) / Xgwm154 / (GA)imp / TCA CAG AGA GAG AGG GAG GG / ATG TGT ACA TGT TGC CTG CA / Roder et al. 1998
(3a) / Xgwm155 / CT / CAA TCA TTT CCC CCT CCC / AAT CAT TGG AAA TCC ATA TGC C / Roder et al. 1998
(4a) / Xgwm160 / GA / TTC AAT TCA GTC TTG GCT TGG / CTG CAG GAA AAA AAG TAC ACC C / Roder et al. 1998
(3a) / Xgwm162 / (CA)AA(CA) / AGT GGA TCG ACA AGG CTC TG / AGA AGA AGC AAA GCC TTC CC / Roder et al. 1998
(6a) / Xgwm169 / GA / ACC ACT GCA GAG AAC ACA TAC G / GTG CTC TGC TCT AAG TGT GGG / Roder et al. 1998
(5a) / Xgwm186 / GA / GCA GAG CCT GGT TCA AAA AG / CGC CTC TAG CGA GAG CTA TG / Roder et al. 1998
(6b) / Xgwm219 / GAimp / GAT GAG CGA CAC CTA GCC TC / GGG GTC CGA GTC CAC AAC / Roder et al. 1998
(4b) / Xgwm251 / CA / CAA CTG GTT GCT ACA CAA GCA / GGG ATG TCT GTT CCA TCT TAG / Roder et al. 1998
(7a) / Xgwm332 / GA / AGC CAG CAA GTC ACC AAA AC / AGT GCT GGA AAG AGT AGT GAA GC / Roder et al. 1998
(3b) / Xgwm340 / GA / GCA ATC TTT TTT CTG ACC ACG / ACG AGG CAA GAA CAC ACA TG / Roder et al. 1998
(6b) / Xgwm361 / GAimp / GTA ACT TGT TGC CAA AGG GG / ACA AAG TGG CAA AAG GAG ACA / Roder et al. 1998
(3b) / Xgwm389 / (CT)(GT) / ATC ATG TCG ATC TCC TTG ACG / TGC CAT GCA CAT TAG CAG AT / Roder et al. 1998
(5b) / Xgwm408 / (CA)(TA)
(CA)(TA) / TCG ATT TAT TTG GGC CAC TG / GTA TAA TTC GTT CAC AGC ACG C / Roder et al. 1998
(2b) / Xgwm429 / CT / TTG TAC ATT AAG TTC CCA TTA / TTT AAG GAC CTA CAT GAC AC / Roder et al. 1998
(4b) / Xgwm513 / CA / ATC CGT AGC ACC TAC TGG TCA / GGT CTG TTC ATG CCA CAT TG / Roder et al. 1998
(5b) / Xgwm540 / (CT)(CC)
(CT) / TCT CGC TGT GAA ATC CTA TTT C / AGG CAT GGA TAG AGG GGC / Roder et al. 1998
(2a) / Xgwm558 / CA / GGG ATT GCA TAT GAG ACA ACG / TGC CAT GGT TGT AGT AGC CA / Roder et al. 1998
(7b) / Xgwm577 / (CA)(TA) / ATG GCA TAA TTT GGT GAA ATT G / TGT TTC AAG CCC AAC TTC TAT T / Roder et al. 1998
(4a) / Xgwm601 / CT / ATC GAG GAC GAC ATG AAG GT / TTA AGT TGC TGC CAA TGT TCC / Roder et al. 1998
Supplementary Table 1c; Chloroplast SSR primers
Marker / Repeat / Forward Primer5’ to 3’ / Reverse Primer
5’ to 3’ / Primer reference
WCt2 / T / cttatctaatgacccaggacgg / cgaattggaaagaattctgacc / Ishii et al. 2001
WCt12 / T / cgatccctatgtagaaagccc / aacgaaaccccttcttaccg / Ishii et al. 2001
WCt13 / A / tgaaaatcctcgtgtcacca / tgtatcacaatccatctcgagg / Ishii et al. 2001
WCt15 / T / caatctggttttgcctggtt / atggggttttctattgatgcc / Ishii et al. 2001
WCt22 / T / gcaatagtgtccttgcccat / accaaaatagtttcattagctcctg / Ishii et al. 2001
Supplementary Table 1d.Wheat Chloroplast primer sequences external to the microsatellite markers
Fragment name / Forward primer 5’ to 3’ / Reverse primer 5’ to 3’WCt2 external / ccatcctagtggatccgtgtgg / cgtggagagatggctgagtgg
WCt12 external / gggatctgatactacaccgc / gggaggagccgtatgagatg
WCt13 external / ccatgcacaatgcataggactacc / gcattccagatagactgagc
WCt15 external / cccgtgcttccagacatgct / catctcgtaaagttccgcgctcgc
WCt22 external / gggtcggctgatacgctt / ccgctatgatgacaatgctgc