Supplementary Table.1.Strains, plasmids, and primers in this experiments.

Strain, plasmid, and primer / Relevant information / Source or reference
Bacterial strains
E. coli strains
DH5α / F- φ80lacZ M15 endArecAhsdR(rk-mk-) supEthi gyrArelA Δ(lacZYA-argF)U169 / Laboratory stock
BL21(DE3) / F- ompT hsdSB(rB-mB-) gal dcm / Novagen
JM110 / dam-, dcm- / Laboratory stock
BW25113 / K12 derivative: ∆araBAD, ∆rhaBAD / (Gust et al., 2003)
ET12567 / nonmethylating E. coli donor with pUZ8002 / (Kieser et al., 2000)

S. coelicolor

A3(2) M145 / SCP1-, SCB2-,Pgl+ / KCTC
Plasmids
pIBR25 / pWHM3 carrying ermE* promoter (EcoRI/ KpnI) from Saccharopolyspora erythraea / (Thuy et al., 2005)
pIJ773 /

aac(3)IV(ApraR)+oriT

/ (Gust et al., 2003)
Primers
6808 F / aaa/aaa/gaa/ttc/ctg/ccc/aac/atc/agg/tga
6808R / aaa/aaa/aag/ctt/cgg/atc/cgg/cgc/agt/cct
0608F / aaa/aaa/gga/tcc/agg/gcg/gcc/ata/cgt/ggt/
0608R / aaa/aaa/aag/ctt/tca/gct/gcc/gtt/ccg/cgc/
6722F / aaa/aaa/gga/tcc/cgc/tcg/tcg/tgt/gca/cgg
6722R / aaa/aaa/aag/ctt/tca/gca/gct/ccc/gcg/cat
3579F / aaa/aaa/gga/tcc/gtt/gcg/ctg/tgc/cca/cct
3579R / aaa/aaa/aag/ctt/tca/gcc/cac/ggc/cgc/gta
Del 0608 F / ctt/cac/ggc/cgc/gaa/cca/agg/aga/gtc/aac/acc/atg/att/ccg/ggg/atc/cgt/cga/cc
Del 0608 R / tcg/ctc/agc/tac/cgc/tca/ctc/tcg/ctc/ggc/tgt/cgc/tca/tgt/agg/ctg/gag/ctg/ctt/c
Del 6808 F / gtc/tat/ggt/tga/cgg/gtg/act/gtc/ata/gat/ctg/cag/atg/att/ccg/ggg/atc/cgt/cga/cc
Del 6808 R / gtc/atc/ttc/cga/acg/gag/atg/gag/gga/gat/ccg/gaa/tca/ tgt/agg/ctg/gag/ctg/ctt/c
Del 1699 F / ggt/ggt/gct/cct/gtc/acc/gag/agg/ggc/gaa/caa/gcg/atg/att/ccg/ggg/atc/cgt/cga/cc
Del 1699 R / caa/gcc/ggt/gtg/cgt/cgc/cga/gtc/ggt/ctc/gcg/cta/cta/tgt/agg/ctg/gag/ctg/ctt/c

Supplementary Table.2.Genes in ACT and RED operons used in these experiments (Huang et al., 2001).

# / SCO # / Gene / Function / 1 / 2 / 3 / 4 / 5 / 6 / 7 / 8 / 9
C1 / SCO5081 / ACTVA 6 / hypothetical protein. / 0.248 / 0.244 / 0.362 / -0.204 / -0.034 / -0.752 / -0.529 / 2.039 / 2.848
C2 / SCO5080 / ACTVA 5 / putative hydrolase / -0.089 / 0.091 / 0.248 / 0.124 / 0.129 / 0.419 / -0.165 / 3.036 / 3.472
C3 / SCO5078 / ACTVA 3 / hypothetical protein / -1.203 / -0.091 / -0.331 / 0.061 / 0.242 / 0.088 / 0.11 / 4.485 / 4.133
C4 / SCO5077 / ACTVA 2 / hypothetical protein / -0.138 / 0.29 / 0.418 / 0.23 / 0.093 / 0.318 / 0.499 / 1.245 / 2.086
C5 / SCO5076 / ACTVA 1 / integral membrane protein / -0.139 / -0.148 / -0.217 / 0.164 / 0.179 / 0.436 / 0.224 / 3.856 / 3.782
C6 / SCO5880 / REDY / RedY protein / 0.425 / 0.399 / 0.448 / 2.724 / 2.571 / 0.981 / 1.531 / 3.317 / 2.166
C7 / SCO5878 / REDX / polyketide synthase RedX / -0.818 / -0.327 / -0.082 / 1.902 / 2.13 / 1 / 1.071 / 2.341 / 1.937
C8 / SCO5879 / REDW / acyl-coa dehydrogenase RedW / 0.381 / 0.026 / 0.077 / 3.002 / 1.437 / 0.856 / 1.948 / 2.652 / 1.984
C9 / SCO5882 / REDV / RedV protein / -0.413 / -0.624 / -0.424 / 0.331 / 0.018 / 0.581 / 0.126 / 0.858 / 0.732
C10 / SCO5881 / REDZ / transcriptional regulator RedZ / 0.129 / 0.516 / 1.622 / 2.37 / 2.66 / 2.645 / 1.994 / 2.463 / 2.693

Legends of Supplementary Figures

Supple. Fig.1.The changes in mRNA-expression levels of ACT (A) and RED (B) genes. Gene expression data are obtained from website( (Huang et al., 2001).

Supple. Fig.2.Color changes of the wild type M145 and SCO0608 overexpressed strain and deletion-mutant in Difco nutrient liquid media after 4 days of cell culture (A), and the cell growth, and ACT production profiles: Cell growth (B), ACT production (C) and specific ACT production (D) in Difco nutrient liquid media. AU, arbitrary units. Wild type M145 (●), SCO0608 overexpressed strain (○) and SCO0608 deletion mutant (▼).

Supple. Fig.3.Different antibiotic production of wild type strain (A), SCO6808 deletion mutant (B) and SCO1699 deletion mutant (C). Using two 96wells (192 wells) with different carbon and nitrogen sources, the patterns of antibiotic production were observed.

Supple. Figure 1.

Supple. Figure 2.

Supple. Figure3.