Supplementary Table 1. Identification of target genes of intermediary metabolism in barramundi. Assembled sequences were compared against known sequences in the database. For each barramundi gene, the correspondingGenbank BLAST hit, e-value probability of the BLAST hit being a random hit and % amino acid matches of each pairwise comparison are shown.

Target Gene / Gene Abbreviation / Genbank Hit / Description / E-value / % positive
Glucose Metabolism
Glucokinase (Hexokinase IV) / Lc GK / AAC33585 / glucokinase [Sparus aurata] / 0 / 98
Glucose-6-Phosphatase / Lc G6Pase / ADP37003 / glucose-6-phosphatase [Lateolabrax japonicus] / 0 / 99
Fructose-1,6-Bisphosphatase / Lc F1,6BPase / AFC36525 / fructose-1,6-bisphosphatase [Lateolabrax japonicus] / 0 / 99
Pyruvate Kinase / Lc PK / XP_003451042 / PREDICTED: pyruvate kinase isozymes R/L [Oreochromis niloticus] / 0 / 97
Phosphoenolpyruvate Carboxykinase, cytosolic / Lc cPEPCK / ACV32405 / cytosolic phosphoenolpyruvate carboxykinase [Rachycentron canadum] / 0 / 98
Phosphoenolpyruvate Carboxykinase, mitochondrial / Lc mPEPCK / ADD37845 / mitochondrial phosphoenolpyruvate carboxykinase [Lateolabrax japonicus] / 0 / 96
Glucose-6-Phosphate Dehydrogenase / Lc G6PDH / XP_004070860 / PREDICTED: glucose-6-phosphate 1-dehydrogenase-like, partial [Oryzias latipes] / 0 / 94
Pyruvate Dehydrogenase Kinase / Lc PDK / ACB72248 / pyruvate dehydrogenase kinase 4 [Fundulus heteroclitus] / 8.18E-17 / 92
Fatty Acid Metabolism
Fatty Acid Synthase / Lc FAS / ACZ55139 / fatty acid synthase, partial [Rachycentron canadum] / 0 / 96
Stearoyl CoA Desaturase / Lc SCD / AFP97552 / stearoyl-CoA desaturase 1b [Sparus aurata] / 0 / 93
CarnitinePalmitoyltransferase / Lc CPT1A / CBN81352 / Carnitine O-palmitoyltransferase 1, liver isoform [Dicentrarchus labrax] / 5.35E-52 / 88
ATP Citrate Lyase / Lc acyl / XP_004078295 / PREDICTED: ATP-citrate synthase-like [Oryzias latipes] / 2.57E-78 / 94
3-hydroxybutyrate dehydrogenase / Lc BDH2 / XP_003454250 / PREDICTED: 3-hydroxybutyrate dehydrogenase type 2-like [Oreochromis niloticus] / 6.93E-94 / 98
hydroxyacyl-coenzyme A dehydrogenase / Lc HOAD / XP_004065945 / PREDICTED: hydroxyacyl-coenzyme A dehydrogenase, mitochondrial-like isoform 1 [Oryzias latipes] / 0 / 95
Amino Acid Metabolism
Alanine Aminotransferase, cytosolic form / Lc cALAT / CBN81871 / Alanine aminotransferase 2-like [Dicentrarchus labrax] / 1.89E-127 / 96
Aspartate Aminotransferase / Lc ASAT / XP_003454182 / PREDICTED: aspartate aminotransferase, cytoplasmic-like [Oreochromis niloticus] / 1.93E-106 / 99
Glutamate Dehydrogenase / Lc GLUD1 / XP_003441805 / PREDICTED: glutamate dehydrogenase, mitochondrial-like [Oreochromis niloticus] / 0 / 99
Citrate Synthase / Lc CS / Q6S9V7 / RecName: Full=Citrate synthase, mitochondrial; Flags: Precursor >gi|41058936|gb|AAR98860.1| mitochondrial citrate synthase precursor [Katsuwonus pelamis] / 4.02E-92 / 100
Glycogen Metabolism
Glycogen Synthase, liver isoform / Lc gys2 / XP_003456075 / PREDICTED: glycogen [starch] synthase, liver-like [Oreochromis niloticus] / 2.51E-142 / 98
Glycogen Phosphorylase, liver isoform / Lc pygl / XP_003442910 / PREDICTED: glycogen phosphorylase, liver form-like isoform 1 [Oreochromis niloticus] / 0 / 99
Nuclear Receptors
Sterol Regulatory Element Binding Protein 1c / Lc SREBP-1c / XP_004558426 / PREDICTED: sterol regulatory element-binding protein 1-like [Maylandia zebra] / 1.47E-21 / 95
Peroxisome Proliferator-Activated Receptor alpha / Lc PPARa / XP_003450741 / PREDICTED: peroxisome proliferator-activated receptor alpha-like isoform 1 [Oreochromis niloticus] / 3.93E-87 / 95
Liver X Receptor / Lc LXR / AFK08803 / liver X receptor, partial [Sebastiscus marmoratus] / 1.14E-101 / 99
Farnesoid X Receptor / Lc FXR / BAN17451 / farnesoid X receptor [Paralichthys olivaceus] / 1.23E-29 / 96