Supplementary Table 1. Identification of Target Genes of Intermediary Metabolism in Barramundi
Supplementary Table 1. Identification of target genes of intermediary metabolism in barramundi. Assembled sequences were compared against known sequences in the database. For each barramundi gene, the correspondingGenbank BLAST hit, e-value probability of the BLAST hit being a random hit and % amino acid matches of each pairwise comparison are shown.
Target Gene / Gene Abbreviation / Genbank Hit / Description / E-value / % positiveGlucose Metabolism
Glucokinase (Hexokinase IV) / Lc GK / AAC33585 / glucokinase [Sparus aurata] / 0 / 98
Glucose-6-Phosphatase / Lc G6Pase / ADP37003 / glucose-6-phosphatase [Lateolabrax japonicus] / 0 / 99
Fructose-1,6-Bisphosphatase / Lc F1,6BPase / AFC36525 / fructose-1,6-bisphosphatase [Lateolabrax japonicus] / 0 / 99
Pyruvate Kinase / Lc PK / XP_003451042 / PREDICTED: pyruvate kinase isozymes R/L [Oreochromis niloticus] / 0 / 97
Phosphoenolpyruvate Carboxykinase, cytosolic / Lc cPEPCK / ACV32405 / cytosolic phosphoenolpyruvate carboxykinase [Rachycentron canadum] / 0 / 98
Phosphoenolpyruvate Carboxykinase, mitochondrial / Lc mPEPCK / ADD37845 / mitochondrial phosphoenolpyruvate carboxykinase [Lateolabrax japonicus] / 0 / 96
Glucose-6-Phosphate Dehydrogenase / Lc G6PDH / XP_004070860 / PREDICTED: glucose-6-phosphate 1-dehydrogenase-like, partial [Oryzias latipes] / 0 / 94
Pyruvate Dehydrogenase Kinase / Lc PDK / ACB72248 / pyruvate dehydrogenase kinase 4 [Fundulus heteroclitus] / 8.18E-17 / 92
Fatty Acid Metabolism
Fatty Acid Synthase / Lc FAS / ACZ55139 / fatty acid synthase, partial [Rachycentron canadum] / 0 / 96
Stearoyl CoA Desaturase / Lc SCD / AFP97552 / stearoyl-CoA desaturase 1b [Sparus aurata] / 0 / 93
CarnitinePalmitoyltransferase / Lc CPT1A / CBN81352 / Carnitine O-palmitoyltransferase 1, liver isoform [Dicentrarchus labrax] / 5.35E-52 / 88
ATP Citrate Lyase / Lc acyl / XP_004078295 / PREDICTED: ATP-citrate synthase-like [Oryzias latipes] / 2.57E-78 / 94
3-hydroxybutyrate dehydrogenase / Lc BDH2 / XP_003454250 / PREDICTED: 3-hydroxybutyrate dehydrogenase type 2-like [Oreochromis niloticus] / 6.93E-94 / 98
hydroxyacyl-coenzyme A dehydrogenase / Lc HOAD / XP_004065945 / PREDICTED: hydroxyacyl-coenzyme A dehydrogenase, mitochondrial-like isoform 1 [Oryzias latipes] / 0 / 95
Amino Acid Metabolism
Alanine Aminotransferase, cytosolic form / Lc cALAT / CBN81871 / Alanine aminotransferase 2-like [Dicentrarchus labrax] / 1.89E-127 / 96
Aspartate Aminotransferase / Lc ASAT / XP_003454182 / PREDICTED: aspartate aminotransferase, cytoplasmic-like [Oreochromis niloticus] / 1.93E-106 / 99
Glutamate Dehydrogenase / Lc GLUD1 / XP_003441805 / PREDICTED: glutamate dehydrogenase, mitochondrial-like [Oreochromis niloticus] / 0 / 99
Citrate Synthase / Lc CS / Q6S9V7 / RecName: Full=Citrate synthase, mitochondrial; Flags: Precursor >gi|41058936|gb|AAR98860.1| mitochondrial citrate synthase precursor [Katsuwonus pelamis] / 4.02E-92 / 100
Glycogen Metabolism
Glycogen Synthase, liver isoform / Lc gys2 / XP_003456075 / PREDICTED: glycogen [starch] synthase, liver-like [Oreochromis niloticus] / 2.51E-142 / 98
Glycogen Phosphorylase, liver isoform / Lc pygl / XP_003442910 / PREDICTED: glycogen phosphorylase, liver form-like isoform 1 [Oreochromis niloticus] / 0 / 99
Nuclear Receptors
Sterol Regulatory Element Binding Protein 1c / Lc SREBP-1c / XP_004558426 / PREDICTED: sterol regulatory element-binding protein 1-like [Maylandia zebra] / 1.47E-21 / 95
Peroxisome Proliferator-Activated Receptor alpha / Lc PPARa / XP_003450741 / PREDICTED: peroxisome proliferator-activated receptor alpha-like isoform 1 [Oreochromis niloticus] / 3.93E-87 / 95
Liver X Receptor / Lc LXR / AFK08803 / liver X receptor, partial [Sebastiscus marmoratus] / 1.14E-101 / 99
Farnesoid X Receptor / Lc FXR / BAN17451 / farnesoid X receptor [Paralichthys olivaceus] / 1.23E-29 / 96