Supplementary Table 1. Detailed Information on the MS/MS Sequencing of the Identified

Supplementary Table 1. Detailed information on the MS/MS sequencing of the identified protein spots.

(1) Spot 1: CALR

Scanf / Rank / Charge / Ions / dCn / XCorr / Reference / Peptide
0803 / 1 / 2 / 19/22 / 0.5184 / 3.9093 / UPSP:CRTC_HUMAN / K.EQFLDGDGWTSR.W
0425 / 1 / 3 / 24/68 / 0.3923 / 3.7648 / UPSP:CRTC_HUMAN / K.FYGDEEKDKGLQTSQDAR.F
0894 / 1 / 2 / 17/20 / 0.3445 / 3.8655 / UPSP:CRTC_HUMAN / K.GQTLVVQFTVK.H
0287 / 1 / 2 / 19/24 / 0.3444 / 3.9764 / UPSP:CRTC_HUMAN / K.HEQNIDCGGGYVK.L
0644 / 1 / 3 / 24/92 / 0.2844 / 3.3840 / UPSP:CRTC_HUMAN / K.IDDPTDSKPEDWDKPEHIPDPDAK.K
1298 / 1 / 3 / 28/80 / 0.3813 / 3.4831 / UPSP:CRTC_HUMAN / K.IDNSQVESGSLEDDWDFLPPK.K
1078 / 1 / 3 / 30/84 / 0.3634 / 4.4546 / UPSP:CRTC_HUMAN / K.IDNSQVESGSLEDDWDFLPPKK.I
0389 / 1 / 2 / 21/28 / 0.4860 / 4.6631 / UPSP:CRTC_HUMAN / K.IKDPDASKPEDWDER.A
0904 / 1 / 3 / 31/92 / 0.3885 / 3.9329 / UPSP:CRTC_HUMAN / K.KPEDWDEEM*DGEWEPPVIQNPEYK.G
0959 / 1 / 3 / 41/116 / 0.5366 / 5.5458 / UPSP:CRTC_HUMAN / K.KPEDWDEEM*DGEWEPPVIQNPEYKGEWKPR.Q
0515 / 1 / 2 / 12/16 / 0.4545 / 2.6249 / UPSP:CRTC_HUMAN / K.KVHVIFNYK.G
1164 / 1 / 3 / 30/120 / 0.4724 / 4.3781 / UPSP:CRTC_HUMAN / K.LFPNSLDQTDM*HGDSEYNIM*FGPDICGPGTK.K
1020 / 1 / 3 / 39/124 / 0.5547 / 5.8487 / UPSP:CRTC_HUMAN / K.LFPNSLDQTDM*HGDSEYNIM*FGPDICGPGTKK.V
1186 / 1 / 3 / 31/120 / 0.1327 / 3.9933 / UPSP:CRTC_HUMAN / K.LFPNSLDQTDM*HGDSEYNIMFGPDICGPGTK.K
1118 / 1 / 3 / 35/120 / 0.4148 / 6.0335 / UPSP:CRTC_HUMAN / K.LFPNSLDQTDMHGDSEYNIM*FGPDICGPGTK.K
0116 / 1 / 1 / 8/10 / 0.1068 / 2.1991 / UPSP:CRTC_HUMAN / K.NVLINK.D
0421 / 1 / 2 / 13/16 / 0.2163 / 2.6131 / UPSP:CRTC_HUMAN / K.NVLINKDIR.C
1558 / 1 / 3 / 40/112 / 0.6115 / 6.4720 / UPSP:CRTC_HUMAN / K.SGTIFDNFLITNDEAYAEEFGNETWGVTK.A
0610 / 1 / 2 / 13/14 / 0.4043 / 2.4850 / UPSP:CRTC_HUMAN / K.VHVIFNYK.G
0623 / 1 / 3 / 39/100 / 0.5486 / 6.3855 / UPSP:CRTC_HUMAN / R.AKIDDPTDSKPEDWDKPEHIPDPDAK.K
1128 / 1 / 3 / 29/88 / 0.5529 / 6.4687 / UPSP:CRTC_HUMAN / R.CKDDEFTHLYTLIVRPDNTYEVK.I
1145 / 1 / 2 / 23/26 / 0.5624 / 5.3948 / UPSP:CRTC_HUMAN / R.FYALSASFEPFSNK.G
UPSP:CALR_HUMAN (P27797; homo sapiens): Calreticulin precursor [MASS=48142]
MLLSVPLLLG LLGLAVAEPA VYFKEQFLDG DGWTSRWIES KHKSDFGKFV LSSGKFYGDE EKDKGLQTSQ DARFYALSAS FEPFSNKGQT LVVQFTVKHE QNIDCGGGYV KLFPNSLDQT DMHGDSEYNI MFGPDICGPG TKKVHVIFNY KGKNVLINKD IRCKDDEFTH LYTLIVRPDN TYEVKIDNSQ VESGSLEDDW DFLPPKKIKD PDASKPEDWD ERAKIDDPTD SKPEDWDKPE HIPDPDAKKP EDWDEEMDGE WEPPVIQNPE YKGEWKPRQI DNPDYKGTWI HPEIDNPEYS PDPSIYAYDN FGVLGLDLWQ VKSGTIFDNF LITNDEAYAE EFGNETWGVT KAAEKQMKDK QDEEQRLKEE EEDKKRKEEE EAEDKEDDED KDEDEEDEED KEEDEEEDVP GQAKDEL
Position / MH+ / Sequence
154-159 / 700.8563 / NVLINK
144-151 / 1020.2194 / VHVIFNYK
154-162 / 1085.2918 / NVLINKDIR
143-151 / 1148.3935 / KVHVIFNYK
88-98 / 1220.4546 / GQTLVVQFTVK
25-36 / 1411.4704 / EQFLDGDGWTSR
99-111 / 1420.5395 / HEQNIDCGGGYVK
74-87 / 1608.7907 / FYALSASFEPFSNK
208-222 / 1801.9073 / IKDPDASKPEDWDER
56-73 / 2088.1950 / FYGDEEKDKGLQTSQDAR
186-206 / 2392.5377 / IDNSQVESGSLEDDWDFLPPK
186-207 / 2520.7118 / IDNSQVESGSLEDDWDFLPPKK
225-248 / 2761.9154 / IDDPTDSKPEDWDKPEHIPDPDAK
163-185 / 2801.1434 / CKDDEFTHLYTLIVRPDNTYEVK
223-248 / 2961.1683 / AKIDDPTDSKPEDWDKPEHIPDPDAK
249-272 / 2962.1704 / KPEDWDEEMDGEWEPPVIQNPEYK
323-351 / 3270.4896 / SGTIFDNFLITNDEAYAEEFGNETWGVTK
112-142 / 3401.7617 / LFPNSLDQTDMHGDSEYNIMFGPDICGPGTK
112-143 / 3529.9358 / LFPNSLDQTDMHGDSEYNIMFGPDICGPGTKK
249-278 / 3716.0293 / KPEDWDEEMDGEWEPPVIQNPEYKGEWKPR

Protein coverage by amino acid count: 262/417 = 62.8%

Protein coverage by mass: 30209/48142 = 62.7%

(2) Spot 2: VIME

Scanf / Rank / Charge / Ions / dCn / XCorr / Reference / Peptide
0529 / 1 / 2 / 17/18 / 0.3900 / 4.5040 / UPSP:VIME_HUMAN / K.FADLSEAANR.N
0636 / 1 / 3 / 27/60 / 0.4443 / 3.1007 / UPSP:VIME_HUMAN / K.FADLSEAANRNNDALR.Q
0516 / 1 / 3 / 25/80 / 0.1916 / 3.5529 / UPSP:VIME_HUMAN / K.TVETRDGQVINETSQHHDDLE.-
0442 / 1 / 2 / 18/30 / 0.3913 / 3.5039 / UPSP:VIME_HUMAN / R.DGQVINETSQHHDDLE.-
0570 / 1 / 2 / 12/16 / 0.1982 / 2.2977 / UPSP:VIME_HUMAN / R.DNLAEDIM*R.L
0634 / 1 / 2 / 15/20 / 0.3283 / 3.1660 / UPSP:VIME_HUMAN / R.EEAENTLQSFR.Q
1077 / 1 / 2 / 19/24 / 0.3906 / 4.8182 / UPSP:VIME_HUMAN / R.KVESLQEEIAFLK.K
0552 / 1 / 2 / 15/18 / 0.5182 / 3.6071 / UPSP:VIME_HUMAN / R.LGDLYEEEM*R.E
0185 / 1 / 2 / 12/18 / 0.2470 / 2.2832 / UPSP:VIME_HUMAN / R.QDVDNASLAR.L
0874 / 1 / 2 / 13/24 / 0.3165 / 3.1016 / UPSP:VIME_HUMAN / R.QVQSLTCEVDALK.G
0944 / 1 / 3 / 25/80 / 0.3708 / 3.8487 / UPSP:VIME_HUMAN / R.QVQSLTCEVDALKGTNESLER.Q
0650 / 1 / 3 / 22/52 / 0.2651 / 3.5502 / UPSP:VIME_HUMAN / R.VEVERDNLAEDIM*R.L
UPSP:VIME_HUMAN (P08670; homo sapiens): Vimentin [MASS=53520]
STRSVSSSSY RRMFGGPGTA SRPSSSRSYV TTSTRTYSLG SALRPSTSRS LYASSPGGVY ATRSSAVRLR SSVPGVRLLQ DSVDFSLADA INTEFKNTRT NEKVELQELN DRFANYIDKV RFLEQQNKIL LAELEQLKGQ GKSRLGDLYE EEMRELRRQV DQLTNDKARV EVERDNLAED IMRLREKLQE EMLQREEAEN TLQSFRQDVD NASLARLDLE RKVESLQEEI AFLKKLHEEE IQELQAQIQE QHVQIDVDVS KPDLTAALRD VRQQYESVAA KNLQEAEEWY KSKFADLSEA ANRNNDALRQ AKQESTEYRR QVQSLTCEVD ALKGTNESLE RQMREMEENF AVEAANYQDT IGRLQDEIQN MKEEMARHLR EYQDLLNVKM ALDIEIATYR KLLEGEESRI SLPLPNFSSL NLRETNLDSL PLVDTHSKRT LLIKTVETRD GQVINETSQH HDDLE
Position / MH+ / Sequence
175-183 / 1077.1974 / DNLAEDIMR
207-216 / 1089.1502 / QDVDNASLAR
294-303 / 1094.1692 / FADLSEAANR
145-154 / 1255.3851 / LGDLYEEEMR
196-206 / 1324.3898 / EEAENTLQSFR
321-333 / 1434.6477 / QVQSLTCEVDALK
222-234 / 1534.7930 / KVESLQEEIAFLK
170-183 / 1689.8811 / VEVERDNLAEDIMR
294-309 / 1777.8911 / FADLSEAANRNNDALR
450-465 / 1837.8540 / DGQVINETSQHHDDLE
321-341 / 2321.5646 / QVQSLTCEVDALKGTNESLER
445-465 / 2424.4998 / TVETRDGQVINETSQHHDDLE

Protein coverage by amino acid count: 116/465 = 24.9%

Protein coverage by mass: 13284/53520 = 24.8%

(3) Spot 3: FETUA

Scanf / Rank / Charge / Ions / dCn / XCorr / Reference / Peptide
0778 / 1 / 2 / 22/26 / 0.6295 / 4.3325 / UPSP:FETUA_HUMAN / K.EHAVEGDCDFQLLK.L
0637 / 1 / 2 / 18/20 / 0.5085 / 3.2910 / UPSP:FETUA_HUMAN / K.LDGKFSVVYAK.C
1279 / 1 / 3 / 35/92 / 0.5771 / 5.2204 / UPSP:FETUA_HUMAN / R.AQLVPLPPSTYVEFTVSGTDCVAK.E
0675 / 1 / 2 / 25/38 / 0.6069 / 5.9359 / UPSP:FETUA_HUMAN / R.HTFM*GVVSLGSPSGEVSHPR.K
0764 / 1 / 2 / 20/32 / 0.5115 / 3.5440 / UPSP:FETUA_HUMAN / R.QLKEHAVEGDCDFQLLK.L
1703 / 1 / 3 / 32/112 / 0.5940 / 4.3693 / UPSP:FETUA_HUMAN / R.QPNCDDPETEEAALVAIDYINQNLPWGYK.H
UPSP:FETUA_HUMAN (P02765; homo sapiens): Alpha-2-hs-glycoprotein precursor (fetuin-a) [MASS=39325]
MKSLVLLLCL AQLWGCHSAP HGPGLIYRQP NCDDPETEEA ALVAIDYINQ NLPWGYKHTL NQIDEVKVWP QQPSGELFEI EIDTLETTCH VLDPTPVARC SVRQLKEHAV EGDCDFQLLK LDGKFSVVYA KCDSSPDSAE DVRKVCQDCP LLAPLNDTRV VHAAKAALAA FNAQNNGSNF QLEEISRAQL VPLPPSTYVE FTVSGTDCVA KEATEAAKCN LLAEKQYGFC KATLSEKLGG AEVAVTCTVF QTQPVTSQPQ PEGANEAVPT PVVDPDAPPS PPLGAPGLPP AGSPPDSHVL LAAPPGHQLH RAHYDLRHTF MGVVSLGSPS GEVSHPRKTR TVVQPSVGAA AGPVVPPCPG RIRHFKV
Position / MH+ / Sequence
121-131 / 1227.4461 / LDGKFSVVYAK
107-120 / 1604.7748 / EHAVEGDCDFQLLK
104-120 / 1974.2390 / QLKEHAVEGDCDFQLLK
318-337 / 2082.3418 / HTFMGVVSLGSPSGEVSHPR
188-211 / 2523.9040 / AQLVPLPPSTYVEFTVSGTDCVAK
29-57 / 3308.6001 / QPNCDDPETEEAALVAIDYINQNLPWGYK

Protein coverage by amino acid count: 101/367 = 27.5%

Protein coverage by mass: 11040/39325 = 28.1%

(4) Spot 4: A1AT

Scanf / Rank / Charge / Ions / dCn / XCorr / Reference / Peptide
0530 / 1 / 2 / 11/14 / 0.2407 / 2.5960 / UPSP:A1AT_HUMAN / K.AVLTIDEK.G
0662 / 1 / 2 / 21/30 / 0.5471 / 5.5756 / UPSP:A1AT_HUMAN / K.DTEEEDFHVDQVTTVK.V
1310 / 1 / 2 / 20/26 / 0.5251 / 4.3492 / UPSP:A1AT_HUMAN / K.ITPNLAEFAFSLYR.Q
0831 / 1 / 2 / 14/18 / 0.2628 / 2.9833 / UPSP:A1AT_HUMAN / K.KLSSWVLLM*K.Y
0380 / 1 / 2 / 13/16 / 0.2157 / 3.1459 / UPSP:A1AT_HUMAN / K.KQINDYVEK.G
0764 / 1 / 3 / 29/56 / 0.4737 / 4.2612 / UPSP:A1AT_HUMAN / K.LQHLENELTHDIITK.F
0765 / 1 / 3 / 19/84 / 0.1818 / 3.0795 / UPSP:A1AT_HUMAN / K.LQHLENELTHDIITKFLENEDR.R
0758 / 1 / 2 / 16/18 / 0.3530 / 3.3737 / UPSP:A1AT_HUMAN / K.LSITGTYDLK.S
0910 / 1 / 2 / 13/16 / 0.3595 / 2.5707 / UPSP:A1AT_HUMAN / K.LSSWVLLM*K.Y
0761 / 1 / 2 / 12/18 / 0.1576 / 2.2015 / UPSP:A1AT_HUMAN / K.LVDKFLEDVK.K
0656 / 1 / 2 / 18/20 / 0.3820 / 3.6231 / UPSP:A1AT_HUMAN / K.LVDKFLEDVKK.L
0830 / 1 / 2 / 27/34 / 0.6310 / 5.7318 / UPSP:A1AT_HUMAN / K.LYHSEAFTVNFGDTEEAK.K
0797 / 1 / 3 / 23/72 / 0.3352 / 3.5574 / UPSP:A1AT_HUMAN / K.LYHSEAFTVNFGDTEEAKK.Q
0484 / 1 / 3 / 17/40 / 0.2084 / 3.0701 / UPSP:A1AT_HUMAN / K.RLGM*FNIQHCK.K
0833 / 1 / 2 / 16/18 / 0.3029 / 3.3959 / UPSP:A1AT_HUMAN / K.SVLGQLGITK.V
0270 / 1 / 3 / 21/56 / 0.2072 / 3.2949 / UPSP:A1AT_HUMAN / K.TDTSHHDQDHPTFNK.I
0842 / 1 / 2 / 25/34 / 0.5636 / 6.1613 / UPSP:A1AT_HUMAN / K.VFSNGADLSGVTEEAPLK.L
0506 / 1 / 2 / 12/18 / 0.3081 / 2.9555 / UPSP:A1AT_HUMAN / R.LGM*FNIQHCK.K
0455 / 1 / 2 / 13/14 / 0.3035 / 2.5236 / UPSP:A1AT_HUMAN / R.SASLHLPK.L
1112 / 1 / 3 / 39/92 / 0.5526 / 6.0049 / UPSP:A1AT_HUMAN / R.TLNQPDSQLQLTTGNGLFLSEGLK.L
UPSP:A1AT_HUMAN (P01009; homo sapiens): Alpha-1-antitrypsin precursor [MASS=46737]
MPSSVSWGIL LLAGLCCLVP VSLAEDPQGD AAQKTDTSHH DQDHPTFNKI TPNLAEFAFS LYRQLAHQSN STNIFFSPVS IATAFAMLSL GTKADTHDEI LEGLNFNLTE IPEAQIHEGF QELLRTLNQP DSQLQLTTGN GLFLSEGLKL VDKFLEDVKK LYHSEAFTVN FGDTEEAKKQ INDYVEKGTQ GKIVDLVKEL DRDTVFALVN YIFFKGKWER PFEVKDTEEE DFHVDQVTTV KVPMMKRLGM FNIQHCKKLS SWVLLMKYLG NATAIFFLPD EGKLQHLENE LTHDIITKFL ENEDRRSASL HLPKLSITGT YDLKSVLGQL GITKVFSNGA DLSGVTEEAP LKLSKAVHKA VLTIDEKGTE AAGAMFLEAI PMSIPPEVKF NKPFVFLMIE QNTKSPLFMG KVVNPTQK
Position / MH+ / Sequence
307-314 / 853.0091 / SASLHLPK
360-367 / 889.0367 / AVLTIDEK
325-334 / 1016.2259 / SVLGQLGITK
259-267 / 1077.3703 / LSSWVLLMK
315-324 / 1111.2804 / LSITGTYDLK
179-187 / 1137.2780 / KQINDYVEK
248-257 / 1191.4516 / LGMFNIQHCK
258-267 / 1205.5444 / KLSSWVLLMK
150-159 / 1206.4247 / LVDKFLEDVK
150-160 / 1334.5988 / LVDKFLEDVKK
247-257 / 1347.6391 / RLGMFNIQHCK
50-63 / 1642.8950 / ITPNLAEFAFSLYR
35-49 / 1780.8077 / TDTSHHDQDHPTFNK
284-298 / 1805.0408 / LQHLENELTHDIITK
335-352 / 1835.0209 / VFSNGADLSGVTEEAPLK
226-241 / 1892.9711 / DTEEEDFHVDQVTTVK
161-178 / 2059.1964 / LYHSEAFTVNFGDTEEAK
161-179 / 2187.3705 / LYHSEAFTVNFGDTEEAKK
126-149 / 2575.8782 / TLNQPDSQLQLTTGNGLFLSEGLK
284-305 / 2708.9877 / LQHLENELTHDIITKFLENEDR

Protein coverage by amino acid count: 204/418 = 48.8%

Protein coverage by mass: 23124/46737 = 49.5%

(5) Spot 5: PDIA1

Scanf / Rank / Charge / Ions / dCn / XCorr / Reference / Peptide
0387 / 1 / 2 / 11/14 / 0.2466 / 2.1243 / UPSP:PDIA1_HUMAN / K.ALAPEYAK.A
0422 / 1 / 2 / 12/16 / 0.3143 / 2.5723 / UPSP:PDIA1_HUMAN / K.DHENIVIAK.M
1125 / 1 / 2 / 12/14 / 0.1774 / 2.4845 / UPSP:PDIA1_HUMAN / K.ENLLDFIK.H
0519 / 1 / 1 / 9/14 / 0.1492 / 2.0633 / UPSP:PDIA1_HUMAN / K.FFPASADR.T
1278 / 1 / 3 / 36/132 / 0.5391 / 5.0845 / UPSP:PDIA1_HUMAN / K.FLESGGQDGAGDDDDLEDLEEAEEPDM*EEDDDQK.A
1070 / 1 / 3 / 34/64 / 0.4371 / 5.1426 / UPSP:PDIA1_HUMAN / K.HNQLPLVIEFTEQTAPK.I
1308 / 1 / 2 / 17/30 / 0.3292 / 3.8740 / UPSP:PDIA1_HUMAN / K.IFGGEIKTHILLFLPK.S
0701 / 1 / 3 / 31/60 / 0.3409 / 4.5478 / UPSP:PDIA1_HUMAN / K.IKPHLM*SQELPEDWDK.Q
0580 / 1 / 3 / 32/76 / 0.3046 / 3.8942 / UPSP:PDIA1_HUMAN / K.IKPHLM*SQELPEDWDKQPVK.V
0788 / 1 / 3 / 33/76 / 0.3317 / 4.3269 / UPSP:PDIA1_HUMAN / K.IKPHLMSQELPEDWDKQPVK.V
1102 / 1 / 2 / 23/28 / 0.5234 / 5.4990 / UPSP:PDIA1_HUMAN / K.ILFIFIDSDHTDNQR.I
0831 / 1 / 2 / 20/28 / 0.3796 / 3.8501 / UPSP:PDIA1_HUMAN / K.LGETYKDHENIVIAK.M
0292 / 1 / 2 / 12/16 / 0.2718 / 2.8008 / UPSP:PDIA1_HUMAN / K.LKAEGSEIR.L
0314 / 1 / 2 / 20/22 / 0.2540 / 2.6646 / UPSP:PDIA1_HUMAN / K.M*DSTANEVEAVK.V
0459 / 1 / 2 / 20/22 / 0.4244 / 4.1063 / UPSP:PDIA1_HUMAN / K.MDSTANEVEAVK.V
0735 / 1 / 2 / 14/18 / 0.2702 / 2.9467 / UPSP:PDIA1_HUMAN / K.NFEDVAFDEK.K
0635 / 1 / 2 / 15/20 / 0.2908 / 3.9389 / UPSP:PDIA1_HUMAN / K.NFEDVAFDEKK.N
0436 / 1 / 1 / 13/18 / 0.3382 / 2.4033 / UPSP:PDIA1_HUMAN / K.PESEELTAER.I
1652 / 1 / 3 / 27/96 / 0.4325 / 4.0708 / UPSP:PDIA1_HUMAN / K.QFLQAAEAIDDIPFGITSNSDVFSK.Y
1056 / 1 / 3 / 26/88 / 0.4687 / 5.4907 / UPSP:PDIA1_HUMAN / K.QLAPIWDKLGETYKDHENIVIAK.M
0614 / 1 / 2 / 17/20 / 0.4877 / 3.7806 / UPSP:PDIA1_HUMAN / K.SNFAEALAAHK.Y
1101 / 1 / 3 / 21/92 / 0.2738 / 3.1783 / UPSP:PDIA1_HUMAN / K.TAAESFKGKILFIFIDSDHTDNQR.I
0970 / 1 / 1 / 11/16 / 0.3180 / 2.4564 / UPSP:PDIA1_HUMAN / K.THILLFLPK.S
0741 / 1 / 2 / 21/30 / 0.5983 / 5.1359 / UPSP:PDIA1_HUMAN / K.VDATEESDLAQQYGVR.G
0428 / 1 / 3 / 26/44 / 0.1344 / 3.4135 / UPSP:PDIA1_HUMAN / K.YKPESEELTAER.I
0896 / 1 / 2 / 17/22 / 0.3367 / 3.4982 / UPSP:PDIA1_HUMAN / K.YQLDKDGVVLFK.K
0728 / 1 / 3 / 23/52 / 0.3220 / 3.1191 / UPSP:PDIA1_HUMAN / R.ADAPEEEDHVLVLR.K
1055 / 1 / 1 / 13/18 / 0.3149 / 3.0428 / UPSP:PDIA1_HUMAN / R.EADDIVNWLK.K
0935 / 1 / 2 / 15/20 / 0.2557 / 3.0910 / UPSP:PDIA1_HUMAN / R.EADDIVNWLKK.R
1136 / 1 / 2 / 13/14 / 0.2071 / 2.7935 / UPSP:PDIA1_HUMAN / R.ILEFFGLK.K
0956 / 1 / 2 / 13/16 / 0.1467 / 3.0588 / UPSP:PDIA1_HUMAN / R.ILEFFGLKK.E
0327 / 1 / 2 / 10/12 / 0.2741 / 2.3164 / UPSP:PDIA1_HUMAN / R.ITEFCHR.F
0495 / 1 / 2 / 20/22 / 0.5389 / 4.0355 / UPSP:PDIA1_HUMAN / R.KSNFAEALAAHK.Y
0603 / 1 / 1 / 11/18 / 0.3812 / 2.5298 / UPSP:PDIA1_HUMAN / R.LITLEEEM*TK.Y
0813 / 1 / 2 / 16/18 / 0.4017 / 3.6872 / UPSP:PDIA1_HUMAN / R.LITLEEEMTK.Y
0390 / 1 / 2 / 13/16 / 0.2043 / 2.3967 / UPSP:PDIA1_HUMAN / R.NNFEGEVTK.E
1149 / 1 / 2 / 21/32 / 0.5488 / 5.3931 / UPSP:PDIA1_HUMAN / R.NNFEGEVTKENLLDFIK.H
1518 / 1 / 3 / 32/116 / 0.3691 / 4.8233 / UPSP:PDIA1_HUMAN / R.TGPAATTLPDGAAAESLVESSEVAVIGFFK.D
0326 / 1 / 2 / 11/12 / 0.2435 / 2.2942 / UPSP:PDIA1_HUMAN / R.TLDGFKK.F
0452 / 1 / 1 / 8/16 / 0.3820 / 2.1520 / UPSP:PDIA1_HUMAN / R.TVIDYNGER.T
UPSP:PDIA1_HUMAN (P07237; homo sapiens): Protein disulfide-isomerase precursor [MASS=57116]
MLRRALLCLA VAALVRADAP EEEDHVLVLR KSNFAEALAA HKYLLVEFYA PWCGHCKALA PEYAKAAGKL KAEGSEIRLA KVDATEESDL AQQYGVRGYP TIKFFRNGDT ASPKEYTAGR EADDIVNWLK KRTGPAATTL PDGAAAESLV ESSEVAVIGF FKDVESDSAK QFLQAAEAID DIPFGITSNS DVFSKYQLDK DGVVLFKKFD EGRNNFEGEV TKENLLDFIK HNQLPLVIEF TEQTAPKIFG GEIKTHILLF LPKSVSDYDG KLSNFKTAAE SFKGKILFIF IDSDHTDNQR ILEFFGLKKE ECPAVRLITL EEEMTKYKPE SEELTAERIT EFCHRFLEGK IKPHLMSQEL PEDWDKQPVK VLVGKNFEDV AFDEKKNVFV EFYAPWCGHC KQLAPIWDKL GETYKDHENI VIAKMDSTAN EVEAVKVHSF PTLKFFPASA DRTVIDYNGE RTLDGFKKFL ESGGQDGAGD DDDLEDLEEA EEPDMEEDDD QKAVKDEL
Position / MH+ / Sequence
462-468 / 808.9530 / TLDGFKK
58-65 / 863.0013 / ALAPEYAK
339-345 / 906.0472 / ITEFCHR
445-452 / 911.0050 / FFPASADR
301-308 / 967.1961 / ILEFFGLK
223-230 / 992.1600 / ENLLDFIK
70-78 / 1003.1435 / LKAEGSEIR
214-222 / 1038.1021 / NNFEGEVTK
416-424 / 1039.1765 / DHENIVIAK
453-461 / 1067.1436 / TVIDYNGER
255-263 / 1082.3744 / THILLFLPK
301-309 / 1095.3702 / ILEFFGLKK
32-42 / 1159.2871 / SNFAEALAAHK
329-338 / 1161.2109 / PESEELTAER
121-130 / 1203.3372 / EADDIVNWLK
317-326 / 1207.4248 / LITLEEEMTK
376-385 / 1214.2739 / NFEDVAFDEK
31-42 / 1287.4612 / KSNFAEALAAHK
425-436 / 1294.4194 / MDSTANEVEAVK
121-131 / 1331.5113 / EADDIVNWLKK
376-386 / 1342.4480 / NFEDVAFDEKK
196-207 / 1425.6678 / YQLDKDGVVLFK
327-338 / 1452.5610 / YKPESEELTAER
17-30 / 1593.7338 / ADAPEEEDHVLVLR
410-424 / 1730.9585 / LGETYKDHENIVIAK
82-97 / 1781.8737 / VDATEESDLAQQYGVR
248-263 / 1827.2632 / IFGGEIKTHILLFLPK
286-300 / 1835.0262 / ILFIFIDSDHTDNQR
231-247 / 1966.2444 / HNQLPLVIEFTEQTAPK
351-366 / 1967.2470 / IKPHLMSQELPEDWDK
214-230 / 2011.2389 / NNFEGEVTKENLLDFIK
351-370 / 2419.8011 / IKPHLMSQELPEDWDKQPVK
402-424 / 2683.0794 / QLAPIWDKLGETYKDHENIVIAK
171-195 / 2714.9885 / QFLQAAEAIDDIPFGITSNSDVFSK
277-300 / 2755.0593 / TAAESFKGKILFIFIDSDHTDNQR
133-162 / 2937.2734 / TGPAATTLPDGAAAESLVESSEVAVIGFFK
469-502 / 3759.6762 / FLESGGQDGAGDDDDLEDLEEAEEPDMEEDDDQK

Protein coverage by amino acid count: 373/508 = 73.4%

Protein coverage by mass: 41989/57116 = 73.5%

(6) Spot 8: PRDX2

Scanf / Rank / Charge / Ions / dCn / XCorr / Reference / Peptide
0852 / 1 / 2 / 17/18 / 0.2214 / 3.1290 / UPSP:PRDX2_HUMAN / K.ATAVVDGAFK.E
1892 / 1 / 2 / 20/32 / 0.3963 / 4.5807 / UPSP:PRDX2_HUMAN / K.EGGLGPLNIPLLADVTR.R
1719 / 1 / 3 / 32/92 / 0.2721 / 4.2033 / UPSP:PRDX2_HUMAN / K.LGCEVLGVSVDSQFTHLAWINTPR.K
0046 / 1 / 2 / 13/14 / 0.1396 / 2.2744 / UPSP:PRDX2_HUMAN / K.TDEGIAYR.G
1364 / 1 / 2 / 10/14 / 0.0977 / 2.2999 / UPSP:PRDX2_HUMAN / R.GLFIIDGK.G
0620 / 1 / 2 / 12/16 / 0.0123 / 1.8920 / UPSP:PRDX2_HUMAN / R.IGKPAPDFK.A
1757 / 1 / 2 / 25/34 / 0.5356 / 5.0717 / UPSP:PRDX2_HUMAN / R.KEGGLGPLNIPLLADVTR.R
0992 / 1 / 2 / 15/16 / 0.1959 / 3.0792 / UPSP:PRDX2_HUMAN / R.LSEDYGVLK.T
0904 / 1 / 2 / 16/18 / 0.2698 / 3.3999 / UPSP:PRDX2_HUMAN / R.RLSEDYGVLK.T
0843 / 1 / 2 / 20/24 / 0.3102 / 3.7340 / UPSP:SAMP_HUMAN / K.IVLGQEQDSYGGK.F
UPSP:PRDX2_HUMAN (P32119; homo sapiens): Peroxiredoxin-2 [MASS=21761]
ASGNARIGKP APDFKATAVV DGAFKEVKLS DYKGKYVVLF FYPLDFTFVC PTEIIAFSNR AEDFRKLGCE VLGVSVDSQF THLAWINTPR KEGGLGPLNI PLLADVTRRL SEDYGVLKTD EGIAYRGLFI IDGKGVLRQI TVNDLPVGRS VDEALRLVQA FQYTDEHGEV CPAGWKPGSD TIKPNVDDSK EYFSKHN
Position / MH+ / Sequence
127-134 / 863.0445 / GLFIIDGK
119-126 / 924.9860 / TDEGIAYR
7-15 / 973.1601 / IGKPAPDFK
16-25 / 979.1211 / ATAVVDGAFK
110-118 / 1024.1589 / LSEDYGVLK
109-118 / 1180.3464 / RLSEDYGVLK
92-108 / 1736.0212 / EGGLGPLNIPLLADVTR
91-108 / 1864.1953 / KEGGLGPLNIPLLADVTR
67-90 / 2644.0203 / LGCEVLGVSVDSQFTHLAWINTPR

Protein coverage by amino acid count: 87/197 = 44.2%

Protein coverage by mass: 9314/21761 = 42.8%

(7) Spot 9: ANXA3

Scanf / Rank / Charge / Ions / dCn / XCorr / Reference / Peptide
1451 / 1 / 3 / 25/76 / 0.1913 / 3.9284 / UPSP:ANXA3_HUMAN / K.AGENRWGTDEDKFTEILCLR.S
1130 / 1 / 2 / 14/16 / 0.2485 / 2.6481 / UPSP:ANXA3_HUMAN / K.ALLTLADGR.R
1144 / 1 / 2 / 15/20 / 0.4155 / 3.9653 / UPSP:ANXA3_HUMAN / K.DISQAYYTVYK.K
1019 / 1 / 2 / 18/22 / 0.3697 / 4.2205 / UPSP:ANXA3_HUMAN / K.DISQAYYTVYKK.S
0911 / 1 / 1 / 10/12 / 0.1083 / 2.0647 / UPSP:ANXA3_HUMAN / K.DIVDSIK.G
1775 / 1 / 3 / 33/120 / 0.3739 / 4.8653 / UPSP:ANXA3_HUMAN / K.ELKDDLKGDLSGHFEHLM*VALVTPPAVFDAK.Q
1371 / 1 / 2 / 13/14 / 0.2495 / 3.2607 / UPSP:ANXA3_HUMAN / K.FTEILCLR.S
1700 / 1 / 2 / 20/30 / 0.3729 / 5.1687 / UPSP:ANXA3_HUMAN / K.GAGTNEDALIEILTTR.T
1599 / 1 / 3 / 37/92 / 0.2958 / 4.9700 / UPSP:ANXA3_HUMAN / K.GDLSGHFEHLM*VALVTPPAVFDAK.Q
1137 / 1 / 2 / 17/20 / 0.4645 / 3.7328 / UPSP:ANXA3_HUMAN / K.GIGTDEFTLNR.I
1120 / 1 / 2 / 17/20 / 0.3742 / 3.6184 / UPSP:ANXA3_HUMAN / K.HYGYSLYSAIK.S
1107 / 1 / 3 / 21/44 / 0.2005 / 3.5392 / UPSP:ANXA3_HUMAN / K.KHYGYSLYSAIK.S
0933 / 1 / 3 / 30/64 / 0.3715 / 4.8722 / UPSP:ANXA3_HUMAN / K.KSLGDDISSETSGDFRK.A
1011 / 1 / 2 / 10/12 / 0.2000 / 2.1105 / UPSP:ANXA3_HUMAN / K.LTFDEYR.N
1372 / 1 / 2 / 15/16 / 0.2177 / 3.2372 / UPSP:ANXA3_HUMAN / K.M*LISILTER.S
0736 / 1 / 2 / 12/14 / 0.0528 / 2.0068 / UPSP:ANXA3_HUMAN / K.QDAQILYK.A
1298 / 1 / 2 / 18/24 / 0.3486 / 3.7411 / UPSP:ANXA3_HUMAN / K.SDTSGDYEITLLK.I
1112 / 1 / 2 / 23/28 / 0.4106 / 5.1294 / UPSP:ANXA3_HUMAN / K.SLGDDISSETSGDFR.K
0995 / 1 / 2 / 22/30 / 0.3842 / 4.4862 / UPSP:ANXA3_HUMAN / K.SLGDDISSETSGDFRK.A
1596 / 1 / 3 / 28/72 / 0.0430 / 3.9366 / UPSP:ANXA3_HUMAN / K.SM*KGAGTNEDALIEILTTR.T
0728 / 1 / 3 / 24/44 / 0.2931 / 3.2445 / UPSP:ANXA3_HUMAN / R.DESLKVDEHLAK.Q
1218 / 1 / 2 / 23/30 / 0.4473 / 4.6758 / UPSP:ANXA3_HUMAN / R.DYPDFSPSVDAEAIQK.A
1617 / 1 / 3 / 32/60 / 0.3007 / 4.6545 / UPSP:ANXA3_HUMAN / R.GIGTDEKM*LISILTER.S
1178 / 1 / 3 / 37/76 / 0.3746 / 5.9092 / UPSP:ANXA3_HUMAN / R.GTVRDYPDFSPSVDAEAIQK.A
0993 / 1 / 2 / 15/18 / 0.1447 / 3.5519 / UPSP:ANXA3_HUMAN / R.KALLTLADGR.R
1041 / 1 / 2 / 15/22 / 0.2423 / 3.1139 / UPSP:ANXA3_HUMAN / R.NISQKDIVDSIK.G
1018 / 1 / 2 / 13/16 / 0.3254 / 2.3168 / UPSP:ANXA3_HUMAN / R.NTPAFLAER.L
0983 / 1 / 3 / 25/52 / 0.2208 / 3.2542 / UPSP:ANXA3_HUMAN / R.QM*KDISQAYYTVYK.K
1043 / 1 / 3 / 22/56 / 0.0449 / 3.2654 / UPSP:ANXA3_HUMAN / R.QMKDISQAYYTVYKK.S
0677 / 1 / 2 / 21/24 / 0.4447 / 4.0240 / UPSP:ANXA3_HUMAN / R.RDESLKVDEHLAK.Q
1460 / 1 / 2 / 15/16 / 0.1207 / 3.4181 / UPSP:ANXA3_HUMAN / R.SEIDLLDIR.T
1406 / 1 / 3 / 19/52 / 0.3123 / 3.1130 / UPSP:ANXA3_HUMAN / R.SEIDLLDIRTEFKK.H
1580 / 1 / 2 / 21/28 / 0.3753 / 5.7974 / UPSP:ANXA3_HUMAN / R.WGTDEDKFTEILCLR.S
UPSP:ANXA3_HUMAN (P12429; homo sapiens): Annexin a3 [MASS=36244]
ASIWVGHRGT VRDYPDFSPS VDAEAIQKAI RGIGTDEKML ISILTERSNA QRQLIVKEYQ AAYGKELKDD LKGDLSGHFE HLMVALVTPP AVFDAKQLKK SMKGAGTNED ALIEILTTRT SRQMKDISQA YYTVYKKSLG DDISSETSGD FRKALLTLAD GRRDESLKVD EHLAKQDAQI LYKAGENRWG TDEDKFTEIL CLRSFPQLKL TFDEYRNISQ KDIVDSIKGE LSGHFEDLLL AIVNCVRNTP AFLAERLHRA LKGIGTDEFT LNRIMVSRSE IDLLDIRTEF KKHYGYSLYS AIKSDTSGDY EITLLKICGG DD
Position / MH+ / Sequence
222-228 / 789.9041 / DIVDSIK
154-162 / 930.0921 / ALLTLADGR
210-216 / 944.0319 / LTFDEYR
176-183 / 979.1209 / QDAQILYK
196-203 / 995.2249 / FTEILCLR
248-256 / 1019.1454 / NTPAFLAER
153-162 / 1058.2662 / KALLTLADGR
279-287 / 1074.2192 / SEIDLLDIR
39-47 / 1076.3397 / MLISILTER
263-273 / 1223.3280 / GIGTDEFTLNR
293-303 / 1302.4723 / HYGYSLYSAIK
126-136 / 1351.4987 / DISQAYYTVYK
217-228 / 1360.5503 / NISQKDIVDSIK
164-175 / 1384.5291 / DESLKVDEHLAK
292-303 / 1430.6464 / KHYGYSLYSAIK
304-316 / 1442.5627 / SDTSGDYEITLLK
126-137 / 1479.6728 / DISQAYYTVYKK
163-175 / 1540.7166 / RDESLKVDEHLAK
138-152 / 1586.6091 / SLGDDISSETSGDFR
104-119 / 1674.8484 / GAGTNEDALIEILTTR
279-292 / 1707.9646 / SEIDLLDIRTEFKK
138-153 / 1714.7832 / SLGDDISSETSGDFRK
123-136 / 1738.9961 / QMKDISQAYYTVYK
32-47 / 1777.0862 / GIGTDEKMLISILTER
13-28 / 1782.9013 / DYPDFSPSVDAEAIQK
189-203 / 1827.0619 / WGTDEDKFTEILCLR
137-153 / 1842.9573 / KSLGDDISSETSGDFRK
123-137 / 1867.1702 / QMKDISQAYYTVYKK
101-119 / 2021.2933 / SMKGAGTNEDALIEILTTR
9-28 / 2196.3784 / GTVRDYPDFSPSVDAEAIQK
184-203 / 2354.5994 / AGENRWGTDEDKFTEILCLR
73-96 / 2552.9509 / GDLSGHFEHLMVALVTPPAVFDAK
66-96 / 3394.9106 / ELKDDLKGDLSGHFEHLMVALVTPPAVFDAK

Protein coverage by amino acid count: 244/322 = 75.8%

Protein coverage by mass: 27471/36244 = 75.8%

(8) Spot 13: RAGE

Scanf / Rank / Charge / Ions / dCn / XCorr / Reference / Peptide
1048 / 1 / 2 / 23/28 / 0.4013 / 4.0137 / UPSP:RAGE_HUMAN / K.VLSPQGGGPWDSVAR.V
1197 / 1 / 3 / 28/68 / 0.2373 / 4.0928 / UPSP:RAGE_HUMAN / R.GGDPRPTFSCSFSPGLPR.H
1469 / 1 / 2 / 25/36 / 0.5331 / 5.3971 / UPSP:RAGE_HUMAN / R.HPETGLFTLQSELM*VTPAR.G
1095 / 1 / 2 / 11/14 / 0.1033 / 2.3324 / UPSP:RAGE_HUMAN / R.IGEPLVLK.C
1476 / 1 / 3 / 36/76 / 0.4363 / 5.6499 / UPSP:RAGE_HUMAN / R.RHPETGLFTLQSELM*VTPAR.G
1240 / 1 / 3 / 39/92 / 0.4346 / 5.9261 / UPSP:RAGE_HUMAN / R.VYQIPGKPEIVDSASELTAGVPNK.V
UPSP:RAGE_HUMAN (Q15109; homo sapiens): Advanced glycosylation end product-specific receptor precursor (receptor for advanced glycosylation end products) [MASS=42803]
MAAGTAVGAW VLVLSLWGAV VGAQNITARI GEPLVLKCKG APKKPPQRLE WKLNTGRTEA WKVLSPQGGG PWDSVARVLP NGSLFLPAVG IQDEGIFRCQ AMNRNGKETK SNYRVRVYQI PGKPEIVDSA SELTAGVPNK VGTCVSEGSY PAGTLSWHLD GKPLVPNEKG VSVKEQTRRH PETGLFTLQS ELMVTPARGG DPRPTFSCSF SPGLPRHRAL RTAPIQPRVW EPVPLEEVQL VVEPEGGAVA PGGTVTLTCE VPAQPSPQIH WMKDGVPLPL PPSPVLILPE IGPQDQGTYS CVATHSSHGP QESRAVSISI IEPGEEGPTA GSVGGSGLGT LALALGILGG LGTAALLIGV ILWQRRQRRG EERKAPENQE EEEERAELNQ SEEPEAGESS TGGP
Position / MH+ / Sequence
30-37 / 869.0922 / IGEPLVLK
63-77 / 1526.6921 / VLSPQGGGPWDSVAR
199-216 / 1879.1004 / GGDPRPTFSCSFSPGLPR
180-198 / 2128.4511 / HPETGLFTLQSELMVTPAR
179-198 / 2284.6386 / RHPETGLFTLQSELMVTPAR
117-140 / 2513.8505 / VYQIPGKPEIVDSASELTAGVPNK

Protein coverage by amino acid count: 85/404 = 21.0%

Protein coverage by mass: 8997/42803 = 21.0%

(9) Spot 16: SODM

Scanf / Rank / Charge / Ions / dCn / XCorr / Reference / Peptide
1707 / 1 / 2 / 20/26 / 0.3625 / 4.8313 / UPSP:SODM_HUMAN / K.AIWNVINWENVTER.Y
1380 / 1 / 3 / 27/96 / 0.2090 / 3.9649 / UPSP:SODM_HUMAN / K.FNGGGHINHSIFWTNLSPNGGGEPK.G
1215 / 1 / 3 / 29/52 / 0.2451 / 5.5320 / UPSP:SODM_HUMAN / K.GDVTAQIALQPALK.F
1132 / 1 / 1 / 10/14 / 0.0098 / 2.0421 / UPSP:SODM_HUMAN / K.GELLEAIK.R
0978 / 1 / 2 / 14/16 / 0.0879 / 2.9088 / UPSP:SODM_HUMAN / K.GELLEAIKR.D
0856 / 1 / 2 / 24/28 / 0.4432 / 4.3420 / UPSP:SODM_HUMAN / K.HHAAYVNNLNVTEEK.Y
1683 / 1 / 2 / 25/38 / 0.4976 / 5.6241 / UPSP:SODM_HUMAN / K.LTAASVGVQGSGWGWLGFNK.E
0634 / 1 / 2 / 11/14 / 0.1566 / 2.6326 / UPSP:SODM_HUMAN / K.NVRPDYLK.A
0677 / 1 / 2 / 12/14 / 0.2846 / 2.7424 / UPSP:SODM_HUMAN / K.RDFGSFDK.F
0868 / 1 / 1 / 10/12 / 0.1239 / 2.5744 / UPSP:SODM_HUMAN / R.DFGSFDK.F
1154 / 1 / 2 / 12/16 / 0.2157 / 2.6910 / UPSP:SODM_HUMAN / R.DFGSFDKFK.E
UPSP:SODM_HUMAN (P04179; homo sapiens): Superoxide dismutase [MASS=24722]
MLSRAVCGTS RQLAPALGYL GSRQKHSLPD LPYDYGALEP HINAQIMQLH HSKHHAAYVN NLNVTEEKYQ EALAKGDVTA QIALQPALKF NGGGHINHSI FWTNLSPNGG GEPKGELLEA IKRDFGSFDK FKEKLTAASV GVQGSGWGWL GFNKERGHLQ IAACPNQDPL QGTTGLIPLL GIDVWEHAYY LQYKNVRPDY LKAIWNVINW ENVTERYMAC KK
Position / MH+ / Sequence
124-130 / 815.8578 / DFGSFDK
115-122 / 873.0372 / GELLEAIK
123-130 / 972.0453 / RDFGSFDK
195-202 / 1005.1619 / NVRPDYLK
115-123 / 1029.2247 / GELLEAIKR
124-132 / 1091.2085 / DFGSFDKFK
76-89 / 1425.6682 / GDVTAQIALQPALK
54-68 / 1739.8852 / HHAAYVNNLNVTEEK
203-216 / 1744.9474 / AIWNVINWENVTER
135-154 / 2036.2974 / LTAASVGVQGSGWGWLGFNK
90-114 / 2638.8611 / FNGGGHINHSIFWTNLSPNGGGEPK

Protein coverage by amino acid count: 114/222 = 51.4%

Protein coverage by mass: 12578/24722 = 50.9%

(10) Spot 18: CAH2

Scanf / Rank / Charge / Ions / dCn / XCorr / Reference / Peptide
1612 / 1 / 3 / 29/60 / 0.3248 / 3.9548 / UPSP:CAH2_HUMAN / K.AVQQPDGLAVLGIFLK.V
0463 / 1 / 1 / 8/10 / 0.2909 / 2.0559 / UPSP:CAH2_HUMAN / K.DFPIAK.G
0696 / 1 / 2 / 15/22 / 0.5115 / 2.3966 / UPSP:CAH2_HUMAN / K.EPISVSSEQVLK.F
0393 / 1 / 1 / 9/16 / 0.2776 / 2.0061 / UPSP:CAH2_HUMAN / K.GGPLDGTYR.L
0748 / 1 / 3 / 25/52 / 0.3633 / 3.2928 / UPSP:CAH2_HUMAN / K.KYAAELHLVHWNTK.Y
1077 / 1 / 3 / 33/92 / 0.3655 / 4.7297 / UPSP:CAH2_HUMAN / K.LNFNGEGEPEELM*VDNWRPAQPLK.N
0667 / 1 / 2 / 15/18 / 0.4308 / 2.9452 / UPSP:CAH2_HUMAN / K.SADFTNFDPR.G
0873 / 1 / 2 / 15/16 / 0.3244 / 2.9524 / UPSP:CAH2_HUMAN / K.VVDVLDSIK.T
0807 / 1 / 3 / 30/48 / 0.3565 / 3.6519 / UPSP:CAH2_HUMAN / K.YAAELHLVHWNTK.Y
0965 / 1 / 3 / 35/72 / 0.4818 / 4.8257 / UPSP:CAH2_HUMAN / K.YDPSLKPLSVSYDQATSLR.I
0814 / 1 / 2 / 23/34 / 0.4801 / 6.4355 / UPSP:CAH2_HUMAN / R.ILNNGHAFNVEFDDSQDK.A
0957 / 1 / 3 / 25/84 / 0.3152 / 4.0801 / UPSP:CAH2_HUMAN / R.ILNNGHAFNVEFDDSQDKAVLK.G
1011 / 1 / 3 / 36/96 / 0.3959 / 4.6688 / UPSP:CAH2_HUMAN / R.KLNFNGEGEPEELM*VDNWRPAQPLK.N
1069 / 1 / 3 / 33/84 / 0.6016 / 6.1827 / UPSP:CAH2_HUMAN / R.LIQFHFHWGSLDGQGSEHTVDK.K
0339 / 1 / 2 / 19/22 / 0.5126 / 3.0401 / UPSP:CAH2_HUMAN / R.QSPVDIDTHTAK.Y
UPSP:CAH2_HUMAN (P00918; homo sapiens): Carbonic anhydrase 2 [MASS=29115]
SHHWGYGKHN GPEHWHKDFP IAKGERQSPV DIDTHTAKYD PSLKPLSVSY DQATSLRILN NGHAFNVEFD DSQDKAVLKG GPLDGTYRLI QFHFHWGSLD GQGSEHTVDK KKYAAELHLV HWNTKYGDFG KAVQQPDGLA VLGIFLKVGS AKPGLQKVVD VLDSIKTKGK SADFTNFDPR GLLPESLDYW TYPGSLTTPP LLECVTWIVL KEPISVSSEQ VLKFRKLNFN GEGEPEELMV DNWRPAQPLK NRQIKASFK
Position / MH+ / Sequence
18-23 / 690.8174 / DFPIAK
80-88 / 936.0122 / GGPLDGTYR
158-166 / 988.1693 / VVDVLDSIK
171-180 / 1170.2237 / SADFTNFDPR
27-38 / 1312.4222 / QSPVDIDTHTAK
212-223 / 1316.4943 / EPISVSSEQVLK
113-125 / 1582.8021 / YAAELHLVHWNTK
132-147 / 1670.0048 / AVQQPDGLAVLGIFLK
112-125 / 1710.9762 / KYAAELHLVHWNTK
58-75 / 2064.1753 / ILNNGHAFNVEFDDSQDK
39-57 / 2141.3856 / YDPSLKPLSVSYDQATSLR
58-79 / 2475.7202 / ILNNGHAFNVEFDDSQDKAVLK
89-110 / 2539.7691 / LIQFHFHWGSLDGQGSEHTVDK
227-250 / 2785.1034 / LNFNGEGEPEELMVDNWRPAQPLK
226-250 / 2913.2775 / KLNFNGEGEPEELMVDNWRPAQPLK

Protein coverage by amino acid count: 176/259 = 68.0%

Protein coverage by mass: 19656/29115 = 67.5%

(11) Spot 21: ENOA

Scanf / Rank / Charge / Ions / dCn / XCorr / Reference / Peptide
1449 / 1 / 2 / 26/38 / 0.4718 / 4.9423 / UPSP:ENOA_HUMAN / K.AGYTDKVVIGM*DVAASEFFR.S
1564 / 1 / 3 / 37/100 / 0.0621 / 4.6667 / UPSP:ENOA_HUMAN / K.DATNVGDEGGFAPNILENKEGLELLK.T
1758 / 1 / 2 / 25/40 / 0.3364 / 4.8714 / UPSP:ENOA_HUMAN / K.DYPVVSIEDPFDQDDWGAWQK.F
1550 / 1 / 2 / 22/28 / 0.2989 / 4.8132 / UPSP:ENOA_HUMAN / K.FGANAILGVSLAVCK.A
1371 / 1 / 2 / 25/38 / 0.4342 / 5.9060 / UPSP:ENOA_HUMAN / K.FTASAGIQVVGDDLTVTNPK.R
1305 / 1 / 3 / 33/80 / 0.4001 / 4.2255 / UPSP:ENOA_HUMAN / K.FTASAGIQVVGDDLTVTNPKR.I
0759 / 1 / 2 / 19/26 / 0.3345 / 4.4403 / UPSP:ENOA_HUMAN / K.IDKLM*IEM*DGTENK.S
0578 / 1 / 3 / 23/44 / 0.2886 / 4.1855 / UPSP:ENOA_HUMAN / K.KLNVTEQEKIDK.L
0989 / 1 / 3 / 34/88 / 0.2582 / 4.3349 / UPSP:ENOA_HUMAN / K.KLNVTEQEKIDKLM*IEM*DGTENK.S
1556 / 1 / 2 / 26/32 / 0.4777 / 5.3494 / UPSP:ENOA_HUMAN / K.LAM*QEFM*ILPVGAANFR.E
0938 / 1 / 2 / 21/26 / 0.1426 / 3.3805 / UPSP:ENOA_HUMAN / K.LAQANGWGVM*VSHR.S
0563 / 1 / 2 / 16/20 / 0.1564 / 3.1589 / UPSP:ENOA_HUMAN / K.LM*IEM*DGTENK.S
1023 / 1 / 3 / 29/84 / 0.3361 / 4.6065 / UPSP:ENOA_HUMAN / K.LNVTEQEKIDKLM*IEM*DGTENK.S
0963 / 1 / 2 / 13/14 / 0.1195 / 2.8176 / UPSP:ENOA_HUMAN / K.SCNCLLLK.V
1738 / 1 / 3 / 38/96 / 0.3331 / 5.5343 / UPSP:ENOA_HUMAN / K.SFIKDYPVVSIEDPFDQDDWGAWQK.F
0879 / 1 / 1 / 10/16 / 0.2614 / 1.8792 / UPSP:ENOA_HUMAN / K.TIAPALVSK.K
1454 / 1 / 2 / 22/26 / 0.3998 / 4.9205 / UPSP:ENOA_HUMAN / K.VVIGM*DVAASEFFR.S
1035 / 1 / 2 / 18/24 / 0.3276 / 3.3052 / UPSP:ENOA_HUMAN / K.YDLDFKSPDDPSR.Y
1478 / 1 / 2 / 25/34 / 0.3190 / 5.3083 / UPSP:ENOA_HUMAN / R.AAVPSGASTGIYEALELR.D
1311 / 1 / 2 / 17/24 / 0.0986 / 4.0943 / UPSP:ENOA_HUMAN / R.GNPTVEVDLFTSK.G
1575 / 1 / 3 / 41/116 / 0.3791 / 6.1094 / UPSP:ENOA_HUMAN / R.HIADLAGNSEVILPVPAFNVINGGSHAGNK.L
1941 / 1 / 2 / 24/42 / 0.4819 / 5.9326 / UPSP:ENOA_HUMAN / R.SGETEDTFIADLVVGLCTGQIK.T
0920 / 1 / 2 / 13/16 / 0.1221 / 2.7921 / UPSP:ENOA_HUMAN / R.SGKYDLDFK.S
0932 / 1 / 3 / 31/60 / 0.2913 / 4.0310 / UPSP:ENOA_HUMAN / R.SGKYDLDFKSPDDPSR.Y
UPSP:ENOA_HUMAN (P06733; homo sapiens): Alpha-enolase [MASS=47038]
SILKIHAREI FDSRGNPTVE VDLFTSKGLF RAAVPSGAST GIYEALELRD NDKTRYMGKG VSKAVEHINK TIAPALVSKK LNVTEQEKID KLMIEMDGTE NKSKFGANAI LGVSLAVCKA GAVEKGVPLY RHIADLAGNS EVILPVPAFN VINGGSHAGN KLAMQEFMIL PVGAANFREA MRIGAEVYHN LKNVIKEKYG KDATNVGDEG GFAPNILENK EGLELLKTAI GKAGYTDKVV IGMDVAASEF FRSGKYDLDF KSPDDPSRYI SPDQLADLYK SFIKDYPVVS IEDPFDQDDW GAWQKFTASA GIQVVGDDLT VTNPKRIAKA VNEKSCNCLL LKVNQIGSVT ESLQACKLAQ ANGWGVMVSH RSGETEDTFI ADLVVGLCTG QIKTGAPCRS ERLAKYNQLL RIEEELGSKA KFAGRNFRNP LAK
Position / MH+ / Sequence
335-342 / 894.1351 / SCNCLLLK
71-79 / 900.1063 / TIAPALVSK
253-261 / 1073.1907 / SGKYDLDFK
92-102 / 1281.4817 / LMIEMDGTENK
15-27 / 1407.5634 / GNPTVEVDLFTSK
80-91 / 1445.6561 / KLNVTEQEKIDK
105-119 / 1463.7783 / FGANAILGVSLAVCK
358-371 / 1526.7563 / LAQANGWGVMVSHR
239-252 / 1541.8055 / VVIGMDVAASEFFR
256-268 / 1555.6410 / YDLDFKSPDDPSR
89-102 / 1637.9038 / IDKLMIEMDGTENK
32-49 / 1806.0257 / AAVPSGASTGIYEALELR
253-268 / 1827.9452 / SGKYDLDFKSPDDPSR
162-178 / 1909.3149 / LAMQEFMILPVGAANFR
306-325 / 2034.2738 / FTASAGIQVVGDDLTVTNPK
233-252 / 2177.4800 / AGYTDKVVIGMDVAASEFFR
306-326 / 2190.4613 / FTASAGIQVVGDDLTVTNPKR
372-393 / 2297.5824 / SGETEDTFIADLVVGLCTGQIK
285-305 / 2511.6631 / DYPVVSIEDPFDQDDWGAWQK
81-102 / 2579.9405 / LNVTEQEKIDKLMIEMDGTENK
80-102 / 2708.1146 / KLNVTEQEKIDKLMIEMDGTENK
202-227 / 2745.0152 / DATNVGDEGGFAPNILENKEGLELLK
281-305 / 2987.2514 / SFIKDYPVVSIEDPFDQDDWGAWQK
132-161 / 3013.3828 / HIADLAGNSEVILPVPAFNVINGGSHAGNK

Protein coverage by amino acid count: 277/433 = 64.0%

Protein coverage by mass: 29588/47038 = 62.9%

(12) Spot 22: RAGE

Scanf / Rank / Charge / Ions / dCn / XCorr / Reference / Peptide
1188 / 1 / 2 / 20/28 / 0.4439 / 4.0850 / UPSP:RAGE_HUMAN / K.VLSPQGGGPWDSVAR.V
1211 / 1 / 3 / 30/68 / 0.2469 / 4.2298 / UPSP:RAGE_HUMAN / R.GGDPRPTFSCSFSPGLPR.H
1482 / 1 / 2 / 25/36 / 0.4092 / 4.6373 / UPSP:RAGE_HUMAN / R.HPETGLFTLQSELM*VTPAR.G
1136 / 1 / 2 / 13/14 / 0.2154 / 2.6282 / UPSP:RAGE_HUMAN / R.IGEPLVLK.C
1504 / 1 / 3 / 35/76 / 0.3503 / 5.4356 / UPSP:RAGE_HUMAN / R.RHPETGLFTLQSELM*VTPAR.G
1342 / 1 / 2 / 28/46 / 0.5097 / 5.8378 / UPSP:RAGE_HUMAN / R.VYQIPGKPEIVDSASELTAGVPNK.V
UPSP:RAGE_HUMAN (Q15109; homo sapiens): Advanced glycosylation end product-specific receptor precursor (receptor for advanced glycosylation end products) [MASS=42803]
MAAGTAVGAW VLVLSLWGAV VGAQNITARI GEPLVLKCKG APKKPPQRLE WKLNTGRTEA WKVLSPQGGG PWDSVARVLP NGSLFLPAVG IQDEGIFRCQ AMNRNGKETK SNYRVRVYQI PGKPEIVDSA SELTAGVPNK VGTCVSEGSY PAGTLSWHLD GKPLVPNEKG VSVKEQTRRH PETGLFTLQS ELMVTPARGG DPRPTFSCSF SPGLPRHRAL RTAPIQPRVW EPVPLEEVQL VVEPEGGAVA PGGTVTLTCE VPAQPSPQIH WMKDGVPLPL PPSPVLILPE IGPQDQGTYS CVATHSSHGP QESRAVSISI IEPGEEGPTA GSVGGSGLGT LALALGILGG LGTAALLIGV ILWQRRQRRG EERKAPENQE EEEERAELNQ SEEPEAGESS TGGP
Position / MH+ / Sequence
30-37 / 869.0922 / IGEPLVLK
63-77 / 1526.6921 / VLSPQGGGPWDSVAR
199-216 / 1879.1004 / GGDPRPTFSCSFSPGLPR
180-198 / 2128.4511 / HPETGLFTLQSELMVTPAR
179-198 / 2284.6386 / RHPETGLFTLQSELMVTPAR
117-140 / 2513.8505 / VYQIPGKPEIVDSASELTAGVPNK

Protein coverage by amino acid count: 85/404 = 21.0%

Protein coverage by mass: 8997/42803 = 21.0%

(13) Spot 23: HBB

Scanf / Rank / Charge / Ions / dCn / XCorr / Reference / Peptide
0286 / 1 / 2 / 11/14 / 0.1083 / 2.5611 / UPSP:HBB_HUMAN / -.VHLTPEEK.S
0714 / 1 / 2 / 18/24 / 0.4897 / 4.2377 / UPSP:HBB_HUMAN / K.GTFATLSELHCDK.L
0962 / 1 / 3 / 31/84 / 0.4687 / 4.5570 / UPSP:HBB_HUMAN / K.GTFATLSELHCDKLHVDPENFR.L
0980 / 1 / 2 / 12/16 / 0.2348 / 2.7210 / UPSP:HBB_HUMAN / K.LHVDPENFR.L
0777 / 1 / 2 / 15/16 / 0.5185 / 3.2052 / UPSP:HBB_HUMAN / K.SAVTALWGK.V
0880 / 1 / 3 / 30/84 / 0.1565 / 4.1698 / UPSP:HBB_HUMAN / K.SAVTALWGKVNVDEVGGEALGR.L
1545 / 1 / 2 / 22/30 / 0.1121 / 5.2076 / UPSP:HBB_HUMAN / K.VLGAFSDGLAHLDNLK.G
0674 / 1 / 2 / 19/24 / 0.3802 / 4.8223 / UPSP:HBB_HUMAN / K.VNVDEVGGEALGR.L
0447 / 1 / 2 / 18/22 / 0.3125 / 2.5067 / UPSP:HBB_HUMAN / K.VVAGVANALAHK.Y
0614 / 1 / 2 / 21/26 / 0.4928 / 4.4180 / UPSP:HBB_HUMAN / K.VVAGVANALAHKYH.-
1520 / 1 / 2 / 22/36 / 0.4937 / 4.3636 / UPSP:HBB_HUMAN / R.FFESFGDLSTPDAVM*GNPK.V
1162 / 1 / 2 / 21/36 / 0.4492 / 5.0342 / UPSP:HBB_HUMAN / R.FFESFGDLSTPDAVMGNPK.V
1329 / 1 / 2 / 19/30 / 0.1250 / 4.1066 / UPSP:HBB_HUMAN / R.LLGNVLVCVLAHHFGK.E
1167 / 1 / 2 / 14/18 / 0.2403 / 2.7322 / UPSP:HBB_HUMAN / R.LLVVYPWTQR.F
UPSP:HBB_HUMAN (P68871; homo sapiens): Hemoglobin subunit beta [MASS=15867]
VHLTPEEKSA VTALWGKVNV DEVGGEALGR LLVVYPWTQR FFESFGDLST PDAVMGNPKV KAHGKKVLGA FSDGLAHLDN LKGTFATLSE LHCDKLHVDP ENFRLLGNVL VCVLAHHFGK EFTPPVQAAY QKVVAGVANA LAHKYH
Position / MH+ / Sequence
9-17 / 933.0953 / SAVTALWGK
1-8 / 953.0832 / VHLTPEEK
96-104 / 1127.2450 / LHVDPENFR
133-144 / 1150.3665 / VVAGVANALAHK
31-40 / 1275.5364 / LLVVYPWTQR
18-30 / 1315.4258 / VNVDEVGGEALGR
83-95 / 1422.5958 / GTFATLSELHCDK
133-146 / 1450.6836 / VVAGVANALAHKYH
67-82 / 1670.9058 / VLGAFSDGLAHLDNLK
105-120 / 1721.1167 / LLGNVLVCVLAHHFGK
41-59 / 2060.2857 / FFESFGDLSTPDAVMGNPK
9-30 / 2229.4979 / SAVTALWGKVNVDEVGGEALGR
83-104 / 2530.8176 / GTFATLSELHCDKLHVDPENFR

Protein coverage by amino acid count: 127/146 = 87.0%

Protein coverage by mass: 13758/15867 = 86.7%

(14) Spot 24: PSB1

Scanf / Rank / Charge / Ions / dCn / XCorr / Reference / Peptide
0962 / 1 / 2 / 23/38 / 0.5902 / 6.0131 / UPSP:PSB1_HUMAN / K.AGGSASAM*LQPLLDNQVGFK.N
1203 / 1 / 2 / 22/34 / 0.6088 / 5.4207 / UPSP:PSB1_HUMAN / K.AM*TTGAIAAM*LSTILYSR.R
0605 / 1 / 2 / 14/16 / 0.3550 / 3.1922 / UPSP:PSB1_HUMAN / K.DVFISAAER.D
0501 / 1 / 2 / 13/20 / 0.2203 / 2.9422 / UPSP:PSB1_HUMAN / K.EGIREETVSLR.K
0819 / 1 / 2 / 21/26 / 0.6027 / 5.1228 / UPSP:PSB1_HUMAN / K.GAVYSFDPVGSYQR.D
0638 / 1 / 2 / 20/26 / 0.4320 / 4.0600 / UPSP:PSB1_HUMAN / K.NM*QNVEHVPLSLDR.A
0779 / 1 / 2 / 20/32 / 0.5190 / 4.1751 / UPSP:PSB1_HUMAN / K.TVIGCSGFHGDCLTLTK.I
0482 / 1 / 2 / 15/16 / 0.3063 / 2.4240 / UPSP:PSB1_HUMAN / R.DVYTGDALR.I
1420 / 1 / 3 / 28/68 / 0.3436 / 5.0777 / UPSP:PSB1_HUMAN / R.FFPYYVYNIIGGLDEEGK.G
0531 / 1 / 2 / 17/18 / 0.4001 / 3.6006 / UPSP:PSB1_HUMAN / R.LSEGFSIHTR.D
1335 / 1 / 3 / 26/72 / 0.2049 / 3.9662 / UPSP:PSB1_HUMAN / R.RFFPYYVYNIIGGLDEEGK.G
UPSP:PSB1_HUMAN (P20618; homo sapiens): Proteasome subunit beta type 1 [MASS=26489]
MLSSTAMYSA PGRDLGMEPH RAAGPLQLRF SPYVFNGGTI LAIAGEDFAI VASDTRLSEG FSIHTRDSPK CYKLTDKTVI GCSGFHGDCL TLTKIIEARL KMYKHSNNKA MTTGAIAAML STILYSRRFF PYYVYNIIGG LDEEGKGAVY SFDPVGSYQR DSFKAGGSAS AMLQPLLDNQ VGFKNMQNVE HVPLSLDRAM RLVKDVFISA AERDVYTGDA LRICIVTKEG IREETVSLRK D
Position / MH+ / Sequence
205-213 / 1008.1192 / DVFISAAER
214-222 / 1010.0917 / DVYTGDALR
57-66 / 1147.2761 / LSEGFSIHTR
229-239 / 1289.4313 / EGIREETVSLR
147-160 / 1546.6795 / GAVYSFDPVGSYQR
185-198 / 1652.8657 / NMQNVEHVPLSLDR
78-94 / 1753.0412 / TVIGCSGFHGDCLTLTK
110-127 / 1872.2483 / AMTTGAIAAMLSTILYSR
165-184 / 2005.2963 / AGGSASAMLQPLLDNQVGFK
129-146 / 2125.3851 / FFPYYVYNIIGGLDEEGK
128-146 / 2281.5726 / RFFPYYVYNIIGGLDEEGK

Protein coverage by amino acid count: 141/241 = 58.5%

Protein coverage by mass: 15395/26489 = 58.1%

(15) Spot 27: ANXA1

Scanf / Rank / Charge / Ions / dCn / XCorr / Reference / Peptide
0736 / 1 / 2 / 14/30 / 0.2609 / 2.9391 / UPSP:ANXA1_HUMAN / K.AAYLQETGKPLDETLK.K
0628 / 1 / 2 / 22/32 / 0.5396 / 4.8196 / UPSP:ANXA1_HUMAN / K.AAYLQETGKPLDETLKK.A
1635 / 1 / 2 / 22/28 / 0.5165 / 5.1569 / UPSP:ANXA1_HUMAN / K.ALTGHLEEVVLALLK.T
0524 / 1 / 2 / 18/22 / 0.4113 / 3.3861 / UPSP:ANXA1_HUMAN / K.CATSKPAFFAEK.L
0526 / 1 / 1 / 10/12 / 0.1488 / 2.1709 / UPSP:ANXA1_HUMAN / K.CLTAIVK.C
0472 / 1 / 2 / 18/20 / 0.6015 / 4.0863 / UPSP:ANXA1_HUMAN / K.DITSDTSGDFR.N
1302 / 1 / 2 / 25/36 / 0.4401 / 4.8336 / UPSP:ANXA1_HUMAN / K.DITSDTSGDFRNALLSLAK.G
0644 / 1 / 2 / 28/36 / 0.3589 / 4.5907 / UPSP:ANXA1_HUMAN / K.GDRSEDFGVNEDLADSDAR.A
0950 / 1 / 3 / 30/92 / 0.5537 / 5.1532 / UPSP:ANXA1_HUMAN / K.GGPGSAVSPYPTFNPSSDVAALHK.A
1448 / 1 / 2 / 23/30 / 0.2653 / 5.2651 / UPSP:ANXA1_HUMAN / K.GLGTDEDTLIEILASR.T
1228 / 1 / 3 / 27/52 / 0.4298 / 3.8551 / UPSP:ANXA1_HUMAN / K.GTDVNVFNTILTTR.S
1400 / 1 / 3 / 27/48 / 0.3720 / 4.6172 / UPSP:ANXA1_HUMAN / K.GVDEATIIDILTK.R
1310 / 1 / 2 / 22/26 / 0.4871 / 4.5250 / UPSP:ANXA1_HUMAN / K.GVDEATIIDILTKR.N
0804 / 1 / 1 / 10/16 / 0.4116 / 2.1379 / UPSP:ANXA1_HUMAN / K.ILVALCGGN.-
1521 / 1 / 3 / 28/60 / 0.4293 / 4.2198 / UPSP:ANXA1_HUMAN / K.KALTGHLEEVVLALLK.T
1208 / 1 / 2 / 24/28 / 0.6289 / 5.0316 / UPSP:ANXA1_HUMAN / K.M*YGISLCQAILDETK.G
1179 / 1 / 3 / 35/76 / 0.6306 / 4.8396 / UPSP:ANXA1_HUMAN / K.M*YGISLCQAILDETKGDYEK.I
0646 / 1 / 1 / 13/20 / 0.3261 / 2.4433 / UPSP:ANXA1_HUMAN / K.TPAQFDADELR.A
0645 / 1 / 2 / 18/28 / 0.5691 / 3.9243 / UPSP:ANXA1_HUMAN / K.TPAQFDADELRAAM*K.G
0830 / 1 / 2 / 18/22 / 0.3568 / 3.8267 / UPSP:ANXA1_HUMAN / K.VLDLELKGDIEK.C
0254 / 1 / 2 / 13/14 / 0.3886 / 2.6457 / UPSP:ANXA1_HUMAN / R.ALYEAGER.R
0174 / 1 / 2 / 12/16 / 0.1043 / 2.3109 / UPSP:ANXA1_HUMAN / R.ALYEAGERR.K
0815 / 1 / 2 / 24/28 / 0.5523 / 4.4040 / UPSP:ANXA1_HUMAN / R.DLAKDITSDTSGDFR.N
1119 / 1 / 2 / 23/28 / 0.5398 / 5.6844 / UPSP:ANXA1_HUMAN / R.KGTDVNVFNTILTTR.S
1040 / 1 / 3 / 26/60 / 0.4148 / 3.8749 / UPSP:ANXA1_HUMAN / R.RKGTDVNVFNTILTTR.S
0718 / 1 / 2 / 24/30 / 0.5593 / 5.6428 / UPSP:ANXA1_HUMAN / R.SEDFGVNEDLADSDAR.A
0300 / 1 / 2 / 14/16 / 0.3259 / 2.6341 / UPSP:ANXA1_HUMAN / R.SEIDM*NDIK.A
UPSP:ANXA1_HUMAN (P04083; homo sapiens): Annexin a1 [MASS=38583]
AMVSEFLKQA WFIENEEQEY VQTVKSSKGG PGSAVSPYPT FNPSSDVAAL HKAIMVKGVD EATIIDILTK RNNAQRQQIK AAYLQETGKP LDETLKKALT GHLEEVVLAL LKTPAQFDAD ELRAAMKGLG TDEDTLIEIL ASRTNKEIRD INRVYREELK RDLAKDITSD TSGDFRNALL SLAKGDRSED FGVNEDLADS DARALYEAGE RRKGTDVNVF NTILTTRSYP QLRRVFQKYT KYSKHDMNKV LDLELKGDIE KCLTAIVKCA TSKPAFFAEK LHQAMKGVGT RHKALIRIMV SRSEIDMNDI KAFYQKMYGI SLCQAILDET KGDYEKILVA LCGGN
Position / MH+ / Sequence
262-268 / 747.9714 / CLTAIVK
337-345 / 860.0592 / ILVALCGGN
204-211 / 908.9866 / ALYEAGER
204-212 / 1065.1741 / ALYEAGERR
303-311 / 1065.1834 / SEIDMNDIK
166-176 / 1214.2310 / DITSDTSGDFR
113-123 / 1263.3495 / TPAQFDADELR
269-280 / 1300.5153 / CATSKPAFFAEK
250-261 / 1372.6017 / VLDLELKGDIEK
58-70 / 1388.6011 / GVDEATIIDILTK
58-71 / 1544.7886 / GVDEATIIDILTKR
214-227 / 1551.7398 / GTDVNVFNTILTTR
98-112 / 1606.9462 / ALTGHLEEVVLALLK
162-176 / 1641.7319 / DLAKDITSDTSGDFR
113-127 / 1664.8738 / TPAQFDADELRAAMK
213-227 / 1679.9139 / KGTDVNVFNTILTTR
317-331 / 1685.9911 / MYGISLCQAILDETK
128-143 / 1703.8869 / GLGTDEDTLIEILASR
97-112 / 1735.1203 / KALTGHLEEVVLALLK
188-203 / 1740.7344 / SEDFGVNEDLADSDAR
81-96 / 1778.0123 / AAYLQETGKPLDETLK
212-227 / 1836.1014 / RKGTDVNVFNTILTTR
81-97 / 1906.1864 / AAYLQETGKPLDETLKK
166-184 / 2025.2229 / DITSDTSGDFRNALLSLAK
185-203 / 2069.0624 / GDRSEDFGVNEDLADSDAR
317-336 / 2278.5972 / MYGISLCQAILDETKGDYEK
29-52 / 2357.5848 / GGPGSAVSPYPTFNPSSDVAALHK

Protein coverage by amino acid count: 236/345 = 68.4%

Protein coverage by mass: 25390/38583 = 65.8%

(16) Spot 30: ALDOA

Scanf / Rank / Charge / Ions / dCn / XCorr / Reference / Peptide
0621 / 1 / 2 / 17/22 / 0.3685 / 4.1120 / UPSP:ALDOA_HUMAN / -.PYQYPALTPEQK.K
0561 / 1 / 2 / 18/24 / 0.4589 / 4.0955 / UPSP:ALDOA_HUMAN / -.PYQYPALTPEQKK.E
0100 / 1 / 2 / 12/14 / 0.2175 / 2.1927 / UPSP:ALDOA_HUMAN / K.AAQEEYVK.R
0158 / 1 / 2 / 13/16 / 0.3160 / 3.1127 / UPSP:ALDOA_HUMAN / K.AAQEEYVKR.A
0780 / 1 / 3 / 29/108 / 0.3282 / 4.3351 / UPSP:ALDOA_HUMAN / K.ALSDHHIYLEGTLLKPNM*VTPGHACTQK.F
0310 / 1 / 2 / 11/14 / 0.2329 / 2.3215 / UPSP:ALDOA_HUMAN / K.ELSDIAHR.I
0776 / 1 / 2 / 22/28 / 0.5536 / 5.3204 / UPSP:ALDOA_HUMAN / K.FSHEEIAM*ATVTALR.R
0734 / 1 / 2 / 17/30 / 0.4244 / 2.1915 / UPSP:ALDOA_HUMAN / K.FSHEEIAM*ATVTALRR.T
0428 / 1 / 2 / 13/18 / 0.1670 / 2.6807 / UPSP:ALDOA_HUMAN / K.GGVVGIKVDK.G
0596 / 1 / 2 / 20/26 / 0.6024 / 4.5486 / UPSP:ALDOA_HUMAN / K.GILAADESTGSIAK.R
0824 / 1 / 2 / 26/38 / 0.5953 / 5.3302 / UPSP:ALDOA_HUMAN / K.IGEHTPSALAIM*ENANVLAR.Y
0274 / 1 / 2 / 14/16 / 0.3017 / 3.1593 / UPSP:ALDOA_HUMAN / K.KELSDIAHR.I
0296 / 1 / 2 / 17/28 / 0.2437 / 3.2686 / UPSP:ALDOA_HUMAN / K.RLQSIGTENTEENRR.F
0194 / 1 / 2 / 12/16 / 0.1181 / 2.1030 / UPSP:ALDOA_HUMAN / K.SKGGVVGIK.V
0352 / 1 / 2 / 17/22 / 0.5315 / 3.8139 / UPSP:ALDOA_HUMAN / K.SKGGVVGIKVDK.G
0440 / 1 / 1 / 9/12 / 0.2880 / 2.0255 / UPSP:ALDOA_HUMAN / K.VLAAVYK.A
0903 / 1 / 2 / 22/42 / 0.5556 / 5.1239 / UPSP:ALDOA_HUMAN / K.YTPSGQAGAAASESLFVSNHAY.-
0400 / 1 / 2 / 15/20 / 0.4409 / 3.4387 / UPSP:ALDOA_HUMAN / R.ALANSLACQGK.Y
0866 / 1 / 3 / 27/128 / 0.4275 / 4.7769 / UPSP:ALDOA_HUMAN / R.ALANSLACQGKYTPSGQAGAAASESLFVSNHAY.-
0371 / 1 / 2 / 21/24 / 0.5411 / 4.1337 / UPSP:ALDOA_HUMAN / R.LQSIGTENTEENR.R
0333 / 1 / 2 / 12/26 / 0.2153 / 3.1253 / UPSP:ALDOA_HUMAN / R.LQSIGTENTEENRR.F
0604 / 1 / 2 / 14/16 / 0.3547 / 3.0376 / UPSP:ALDOA_HUMAN / R.QLLLTADDR.V
1185 / 1 / 3 / 39/116 / 0.4227 / 5.2155 / UPSP:ALDOA_HUMAN / R.TVPPAVTGITFLSGGQSEEEASINLNAINK.C
1020 / 1 / 3 / 32/108 / 0.5354 / 6.2261 / UPSP:ALDOA_HUMAN / R.YASICQQNGIVPIVEPEILPDGDHDLKR.C
UPSP:ALDOA_HUMAN (P04075; homo sapiens): Fructose-bisphosphate aldolase a [MASS=39289]
PYQYPALTPE QKKELSDIAH RIVAPGKGIL AADESTGSIA KRLQSIGTEN TEENRRFYRQ LLLTADDRVN PCIGGVILFH ETLYQKADDG RPFPQVIKSK GGVVGIKVDK GVVPLAGTNG ETTTQGLDGL SERCAQYKKD GADFAKWRCV LKIGEHTPSA LAIMENANVL ARYASICQQN GIVPIVEPEI LPDGDHDLKR CQYVTEKVLA AVYKALSDHH IYLEGTLLKP NMVTPGHACT QKFSHEEIAM ATVTALRRTV PPAVTGITFL SGGQSEEEAS INLNAINKCP LLKPWALTFS YGRALQASAL KAWGGKKENL KAAQEEYVKR ALANSLACQG KYTPSGQAGA AASESLFVSN HAY
Position / MH+ / Sequence
208-214 / 763.9555 / VLAAVYK
99-107 / 845.0299 / SKGGVVGIK
322-329 / 938.0252 / AAQEEYVK
14-21 / 941.0317 / ELSDIAHR
101-110 / 972.1729 / GGVVGIKVDK
60-68 / 1045.1807 / QLLLTADDR
13-21 / 1069.2058 / KELSDIAHR
331-341 / 1076.2559 / ALANSLACQGK
322-330 / 1094.2127 / AAQEEYVKR
99-110 / 1187.4252 / SKGGVVGIKVDK
28-41 / 1333.4813 / GILAADESTGSIAK
1-12 / 1435.6196 / PYQYPALTPEQK
43-55 / 1491.5546 / LQSIGTENTEENR
1-13 / 1563.7937 / PYQYPALTPEQKK
43-56 / 1647.7421 / LQSIGTENTEENRR
243-257 / 1676.9282 / FSHEEIAMATVTALR
42-56 / 1803.9296 / RLQSIGTENTEENRR
243-258 / 1833.1157 / FSHEEIAMATVTALRR
153-172 / 2108.4203 / IGEHTPSALAIMENANVLAR
342-363 / 2229.3673 / YTPSGQAGAAASESLFVSNHAY
259-288 / 3059.4001 / TVPPAVTGITFLSGGQSEEEASINLNAINK
215-242 / 3076.5586 / ALSDHHIYLEGTLLKPNMVTPGHACTQK
173-200 / 3121.5352 / YASICQQNGIVPIVEPEILPDGDHDLKR
331-363 / 3286.6000 / ALANSLACQGKYTPSGQAGAAASESLFVSNHAY

Protein coverage by amino acid count: 242/363 = 66.7%

Protein coverage by mass: 25971/39289 = 66.1%